FLCN (AK095390) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK095390, the custom clone sequence may differ by one or more nucleotides TACAAATGCCTCTCGTCAGTGGCAAGCCAGTAACCACCACTCAGAAGAGAATGCAAAACAGGCCGGGCAC AGTGGCTCACACCTGTAATCCCAACACTTTGAGAGGCTGAAGTGGGTGGATCACTTGAGGTCAGGAGTTT GAGACCAGTCTGGCCAACACGGTGAAACCCCGACTCTACTAAAAAATACAAAAATTAGCCAGGCATGGTG GTGCACACCTGTAGTCCCAGTTACTCAGGAGGCTGAGGCAGGAGAATTGCTTGAACTCAGGAGGTGGAGG CTGCAGTGAGCTGAGTTTGCACCATTGTACTCCACCCTGGGTAACAGAGTAAGACTCTGTTTCAAAAAAA GAGAGAATGCAAAACACCTACCCTATGGAAGTGAATCAGCATTGTCATCTTTATATCCTGAGAATAAATG ATTCCATCCATCATCCATCCATCCATTCATCTACCCCTAATGCATGTATTCATTTCAACTTAATTTTCAC CTCCTGGGTGTTTACAACCAACTAGCTACTGAAGGATGAAACACCTCTTTCTTACCCACAATATCTTATT AAAGGAAGAAAGAGAATTATCCCTTTAAAGTCTTATAAACCTTCAGAGATAATGGATTTCCAGGCTCAAG ATAACCTGTAGAACCACATGGAGGGTCTTGTGTTTCCCAGCTCTTTAATTTTCAGACACTTTGATCCCTC CTCAGTTCTCATAAGTACATGATGACCAGGGGAGTTGGTTATTACAATAGCTACCATGCACAAGGGGCAT GAAGACATGGAGAGATGACAATACTTGTCTAGGGCCATCTAGATTGGTGGAATGTGAGAACCTAGAGTTG TTGCTCCCTTGACCTAAGAATGACTCAGTATATTGATAGGCATGTACACCACCCTCAAATCTTTAAACCA TTACCTGCAGGCTCCTTGAATGTATTGAGGCCTCTCCTCTCTGTACTATTTCTGCTTGTCATCATTTTCG TGGTAAGTAATGCCCTTTGCACATTACATTTACATGCAAGAAAGAAGTGTTTCTCAATGGGAGGGTTTAG GAATGAGGGCCAGGCCACAGCAGAAGTCAACCTCAATCCACCAATATCATCTGACACCTCCCTTCCACTA TCCTTTCTCAGGATGGCCTCAGTTGGGGCCGGGGGTGAGGATTTGGATCCGGAGTTTGTCTTTAGTCTCA TGCCTCTTAGCCTCATGCTATCGTGCTTCTATGAGGAACTTACAGGTCATAATTGTAGACAGGATTCTGC TCCTTTCCCACAACACCCTCATGCTGGTTCCCATGCTGTGTCCTGCTCCTGCTTTCTGGAGGTCATCGGG AGCGGGCACTGCAGGCAGGGAGCACTCTGTGGCCTCTGTGACTCCTAGAGCCTGGCTACCTGGAGCCTGA GTGGGCATGAAGGGTGAGTAAGCCGGGGAACTAGGACACGTTAGCTAATGCAGAGCCTGGATCAAACACC ACCACCACCATTTGCCCCCGCTCCCAAATGGTTATAAGCAAAACTCTCCTGTCTTCTGAGGTAGATTTCT TCATCATTACTTCTTTGCCATATGGAAATGTAATTTGACTTTTAGATTTAAATCTAGTAATGATTAATAT ATCAGAATTTACAGTGTTTGCAATGTCAAGGAGGCAACACCTGTAAACAGTTGGTTCAGGAATTGCTAGG AGTCTTGGCCTCTTTCCCTCTTGGTCGCCTGCTCAGCAGCAGCCTGTGTATTTATAGTCCATTATGGGCA TAGGATGTTACCCTAACTGGTGCCCACAGTGAACAAGGGGCTAAGCCCTGTCTCTCGTCTGGTCTTAGGA GGGCTTCTGTTTAATGGGCTGAACTTAAAGGTCCTTCCCTGCAACCCCCACACAGCCACAGCCCATGGGC TGAAAGCTCAGCTGAACTCCCATTGTACCTCCCCAGCTCATCCTAGTGAATGTGCCAGGGCCCAGAGGGA GAGGTCAGGCTGTGCCAGCTCAGTGCTATACTGCAGGCACATGGATCCCCAACCCTGGGCCCTATTTTGA GGCAGTGTGGAGTGGTGGTCAAAGGGCTGGACTCCAGAGACAGACAGTCTTGGTTCAAACCTTGCTGTGT GATCTTGGGCAAATCAGTCCATTTATCACTGTCTCAGTTTCCTTATCTAAGAAATGGAGACCCTAATAGT ACCTACCTTTTAGGGTTGCTAGGAGAAAAGTGCCTGGCACATAGTAAGAACTTACTACATGTTGGTTGTT TTTATGATTATTGTTATATAATTAGAAGAGTGACCAGGCTAGCAGCAGAGAGTCCTCATTCTCAGGAGAG TGGGAAGAGGGAGAGAGGTGAAGTGTCAGAGGGCTGCAGAGCCAATACCTGGCAGAGCCCCAGGCAGCAG GTTTGCCACTTGGGGGGCCTGGGTGGGGCCCAGGTAGCTTTCCTGGTTCTCAGGTCTTCATTTCAGACAG TGCTCTGAGCCTCCCATCCACAGTCCAGAGCCCATTCTCTGCCTGCCTCCTTCTCTACTATTGTCGCCAC CTGCAGTCTTTCTGAGCAGCTGGTTAAGGTGCTACTATTCCTCCTCCCAAGGGTGAGGGCCAGCCCTGAA CAAAAGGGCTCCGGAGGGAGCCACAAAACCAGATAGCCTCAGGTTTCCCAAATCGCCTCTCCTGCTGCCC TGGCTTCTCAACACCGTCACCTGTCTAAAAGCTTTTCTTGGTGTGGGCTGGAAGAATTTGGATAAAGTCT ACCATCCCCGCTCTTCCTTAATCCTATTACCACAGCTTCTTTGCTCCCAGCTCTGTCCAGCTACCTAACC TCAAGGAAGCCAACAGGCGATGGAAAAGTTGGCATAAAAATGAGAATGTAGGCTGGACGTAATCCCACCT GTAATAACACCTGTAATCCCAGCACTTTGGGAGGCCAGGGACGGCAGATTGCTTGAGGCCAGGAGTTTGA GACCAGCCTGGGCAACGTGGTGGGCTCCTGTCTCTACAAAAAATATAAAAATTAGTTGGGCATGAAGGCA TGCACCTGTGGTTTCTGCTACTTCGGAGGCTGAGGTGGAAGATCGTTTGAGCCTGGGATGTCAGAGCTGC AGTGAGCCAAGATTGTGTCACTCCAACCTGGCAACAGAGGAGACCTTGTCTC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK095390 unedited ATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTATGGAAGTGAA TCAGCATTGTCATCTTTATATCCTGAGAATAAATGATTCCATCCATCATCCATCCATCCA TTCATCTACCCCTAATGCATGTATTCATTTCAACTTAATTTTCACCTCCTGGGTGTTTAC AACCAACTAGCTACTGAAGGATGAAACACCTCTTTCTTACCCACAATATCTTATTAAAGG AAGAAAGAGAATTATCCCTTTAAAGTCTTATAAACCTTCAGAGATAATGGATTTCCAGGC TCAAGATAACCTGTAGAACCACATGGAGGGTCTTGTGTTTCCCAGCTCTTTAATTTTCAG ACACTTTGATCCCTCCTCAGTTCTCATAAGTACATGATGACCAGGGGAGTTGGTTATTAC GATAGCTACCATGCACAAGGGGCATGAAGACATGGAGAGATGACAATACTTGTCTAGGGC CATCTAGATTGGTGGAATGTGAGAACCTAGAGTTGTTGCTCCCTTGACCTAAGAATGACT CAGTATATTGATAGGCATGTACACCACCCTCAAATCTTTAAACCATTACCTGCAGGCTCC TTGAATGTATTGAGGCCTCTCCTCTCTGTACTATTTCTGCTTGTCATCATTTTCGTGGTA AGTAATGCCCTTTGCACATTACATTTACATGCAAGAAAGAAGTGTTTCTCAATGGGAGGG TTTAGGAATGAAGGCCANGCCACAGCAGAAGTCAACCTCAATCCACCAATATCATCTGAC ACCTCCCTTCCACTATCCTTTCTCANGATGGCCTCAGTTGGNGCCCGGGGGTGAGATTTN GATCCNGNAGTTGNCTTTATCTCATGCCTCTTAACCTATGCTATCGNGCTCTAT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK095390 unedited GGTACTACTATGNNACCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTGAG ACAGGTCTCCTCTGTTGCCAGGTTGGAGTGACACAATCTTGGCTCACTGCAGCTTTGACA TCCCAGGCTCAAACGATCTTCCACCTCAGCCTCCGAAGTAGCAGAAACCACAGGTGCATG CCTTCATGCCCAACTAATTTTTATATTTTTTGTAGAGACAGGAGCTTACTACGTTGCCCA GGCTGGTCTCAAACTCCTGGCCTCAAGCAATCTGCCGTCCTTGGCCTCCCAAAGTGCTGG GATTACAGGTGTTATTACAGGTGGGATTACGTCCAGCCTACATTCTCATTTTTATGCCAA CTTTTCCATCGCCTGTTGGCTTCCTTGAGGTTAGGTAGCTGGACAGAGCTGGGAGCAAAG AAGCTGTGGTAATAGGATTAAGGAAGAGCGGGGATGGTAGACTTTATCCAAATTCTTCCA GCCCACACCAAGAAAAGCTTTTAGACAGGTGACGGTGTTGAGAAGCCAGGGCAGCAGGAG AGGCGATTTGGGAAACCTGAGGCTATCTGGTTTTGTGGCTCCCTCCGGAGCCCTTTTGTT CAGGGCTGGCCCTCACCCTTGGGAGGAGGAATAGTAGCACCTTAACCAGCTGCTCAGAAA GACTGCAGGTGGGGACAATAGTAGAGAAGGAGGCAGGCAGAGAATGGGCTCTGGACTGTG GATGGGAGGCTCANAACACTGTCTGAAATGAAGACCTGAGAACCAGGAAAGCTACCCTGG GCCCACCCCAGCCCCCCAAGTGGCAAACCTGCTGCCTGGGGCTCTGCCAGTATTGGCTCT GCAGCCCTCTGACACTCACCTCTTTCCCTCTTCCACTCTCCTGAAATGAAGACTCTCTGC TGCTAGCCTGTCACTCCTCTAATATAT
Restriction Sites	NotI-NotI
ACCN	AK095390
Insert Size	3132 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK095390.1
RefSeq Size	3132 bp
RefSeq ORF	3132 bp
Locus ID	201163
Protein Families	Druggable Genome
Protein Pathways	Renal cell carcinoma
Gene Summary	This gene is located within the Smith-Magenis syndrome region on chromosome 17. Mutations in this gene are associated with Birt-Hogg-Dube syndrome, which is characterized by fibrofolliculomas, renal tumors, lung cysts, and pneumothorax. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

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