ARIH1 (AK000336) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ARI; HARI; HHARI; UBCH7BP
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK000336, the custom clone sequence may differ by one or more nucleotides CGACGAAGGGTTTTGTTACAGCATGTGCATGAAGGCTACGAAAAAGATCTGTGGGAGTACATTGAGGACT GAGAATGGCCCTGCATAAAATGAACTCTGAAAACTTTACCATCTAGAGTGCTCATGCAATTAAAACAAAG CAAACACAAACAAGGAGGCACTAAGCCTATTCTGACACCACTGGTCTGTAGTACCAGAATTGTTTTGTTA ATGGAAAGTTTAAGTAAATTATATTGTAATAAAAAGGTAGATAAACCATTGTACAACAGTATTCTAGGCC GCCAACAAAAGTGTGACAGACACACTAAAAGCCCTCCAACTTTAACTTGTAACGTAGCTTCATTCTCAAA GCTGACTCCTTTTTTTTCTTTTTCCTTTTCCTGAGTGTAGTACAGTTAAAATTTCAAACAGCTCCTTGAC ACTGCTTTTCATGTTCAAACCAGCCATTTTGTTGTACTTTGGTAAAGGACCTCTTCCCCTTCCTCCCCTA CACATACAGATACACCCACACACAGACTGACTCTCTTTCTCTCATACCCCAAGGTCATGAGTGAATGATG CTTAGTTCCTTGTAAAGAAAATCTTGGGATGGGGAAAGGGGTAGGCAGCAAGAGGATTCAACAAACGAAA AACATAAAAACTTTGTATATGACTTTTAAAACAAGAGGACAACACAGTATTTTTCAAAATTGTATATAGC GCATATGCATGGACAAAGCAAGCGTGGCACGTGTTTGCATAATGTTTAATTACAAAAAAATATTTATTCT TTAAAAATCTTCAAGATTATGTCTATTTGCTGTGCATTTTCTTTCAGTTTGCTTATCTTTCCCGGGTTGG GGTTGGGATAAAGGTGTGTCGGTTTAGCACCTCTGGAAGACCTATCTAGAGCTCTTTCACTTTCCTGAGG TTATTTTGCCCTTTCTGGTGTTGGTATGTCTGTTGCCGGCCATGGGCCTCATGCCTTGAATTCCTGCTCT TGATCAGGGACAAGGGAGGTCAAGCTCTGACTAATGCCATGACCTGATTAAGGGGTACAGCAGGGAGTTT TGTTGCTACAGCTCATGAATTAACCTGTCCCAACCTAATCCCCCTCCATGGCATCATGCCTCTACCCAAG CCTTTGTGTGCCCATGTTATGCACACAGCTGTAGGCATTCTTAAGTCCCCTGTCGCATCCAGTGGAAGCA TTTTAAAATTTCTTTTACTTTTTGGTTTTCCCTTAATTGCTGCTTTTCAGATTTTAGTTATGGCTCGTCT GCTCACCCCTTCTCTACATTAGGGTGTCAAAGAGAATGTTTTGCTTTAAATATAAATAGCCATTCATTTA GTCTCAGATTGTGAATTTAAAATGGTGGATACCGAAATTGCTTGTGTGTGTTGCTGTGGGTTTGGTTTGA AGGCAAACACCCCTAGAACATGATATTCCCATCTAGTGCATTTAAATAGAAATCACTGAGTTTGCTGCTT TTTTATTGTCAGCAGATAGGAGAATTAATAATGCATTTTAGCTGTGATGTCCATTTTTATGAAATTCCTA CTAAGAGCTATGTTAAAAGTAAAGGATGGTGGTGGTTGTATTAACTATATACCTGTTTAGGCCATTCTGG CTGTGGTATTTTTCAATAGGTCAGCATCTGTAAATCTGTCAGTTTTATACAGGAGTGCAGAGTGAACTAG GCAACTAGATTAAGAGGTCTAAATATGAAATACCAGTTGAGGCTGAGGACCTCTTCGTCTTCCTTTAAAT GTCTTTTGCCTAGGGAGTGTTTACCATTTGTGAGGCAGCTTTGTCTGCTCTTACACTGTACATCCTATTA CTCCATTGGGAAGTAGGTTCACTTTCCTCTGGCCTTTTGCCTAAGTTAGGCTTTGCTGAATCAACCCTAC TTTTCCTTTTAGAAAAGGTTGTTACAGGAGATTTACTGGCAACTGTTCTTTTCCCATCAAAAATCAGTGA ATGTTTGCTGAGTATAAATGCTGCTTCCTTAAACCACTTGTCGCTTTAGGATCAACTTTACCTGTACCTT TTCTCCTTTCCTCCCTTGCCACCTCAGGTGCAAATCTGAACTCAGTGTCTGCTTCTTCCATTTTCTCGTC TCTCTCCCCTCTTCCCCCATTATCCATATGACATTATTTTACTTCAAATGACAGCATCAATCTTAAAAAG ATATACATTAAAACTAAGGAGTTTTTTTAAAGAAAGCCTGAATAAGTTCCTTTCCCTGGTAACTTTGAAA AGCAGTCAGAGTTGCTATATAGATATATGTGGCTCCTTTAAAATGCTTTGTGTATGTGTGGTGTTTAAAA AAAAAAAATCTTACCAGTTAACTTTGCAGTGTCTAGGTTTGGGTGTCATAAATCCACGTGTTCCTGTTGC AACAAATACCCAAAAATTGTGTGTGCACTTCCTAATACCAGTCTTCACCCATGGAGGAACAGTGCTTTTT AGAGATGCTTTCTATTTCAATGTTGGCATACTGCCTGAGGGTATTGCAGTTGTGGGTGCATTCCCTAATT TGTATGATCAAGATGAACTGGCCCTTTTCTACTTCCAAGCTTTTAACAGATACCACCATATTTGACAGAA TTCCCAGAGTGAATTGCTTGTGTTATTAGTAGATTCAGTGCCCCCAGCTGGGATAGGCAAGCCATGACAG CTTCCCTGTTTCACCTACAGAAGTCTTATCTGAGGGATCTATTCACAGTAAGCACCAAGGTCTCCATGTC TTGAGGTCAGTTTCATTGTCTTTTGAAAAGTGCATGCTTCATTTGAACAATTCATTCAGCAGCAGATGGA CTTTCAGTGATTTAAAATAAAATTTTGATCCAAAGCTCAGGACACAAACCACAGTGGTAAAATTGAGTAG CATATAATATCAGACTAAATTATCTGTAATTTTCCACAACCCAGATTGTATGTGTTTTATGTGTGTTTAA ATAAATATGTTAGATACACGTGTATACATACACCCATAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK000336 unedited TTCAAATTTTGTAATACGACTCACTATAGGGCGGCCGCGNAATTCGCACGAGGGCAACTA GATTAAGAGGTCTAAATATGAAATACCAGTTGAGGCTGAGGACCTCTTCGTCTTCCTTTA AATGTCTTTTGCCTAGGGAGTGTTTACCATTTGTGAGGCAGCTTTGTCTGCTCTTACACT GTACATCCTATTACTCCATTGGGAAGTAGGTTCACTTTCCTCTGGCCTTTTGCCTAAGTT AGGCTTTGCTGAATCAACCCTACTTTTCCTTTTAGAAAAGGTTGTTACAGGAGATTTACT GGCAACTGTTCTTTTCCCATCAAAAATCAGTGAATGTTTGCTGAGTATAAATGCTGCTTC CTTAAACCACTTGTCGCTTTAGGATCAACTTTACCTGTACCTTTTCTCCTTTCCTCCCTT GCCACCTCAGGTGCAAATCTGAACTCAGTGTCTGCTTCTTCCATTTTCTCGTCTCTCTCC CCTCTTCCCCCATTATCCATATGACATTATTTTACTTCAAATGACAGCATCAATCTTAAA AAGATATACATTAAAACTAAGGAGTTTTTTTAAAGAAAGCCTGAATAAGTTCCTTTCCCT GGTAACTTTGAAAAGCAGTCAGAGTTGCTATATAGATATATGTGGCTCCTTTAAAATGCT TTGTGTATGTGTGGTGTTTAAAAAAAAAAAATCTTACCAGTTAACTNTGCAGTGTCTAGG TTTGAGTGTCATAATCCACGTGTTCCTGTTGCAACNATACCCNAAATNGTGTGTGCACTT NCTAATACAGTCTTCACCCATGAGGAACAGTGCTTTTAGAGATGCTNTCTATTTCATGNT NGCATACTGCCCTGAGGTATTGCAGTTGTGGGNTGCATTCCCTAATTGNATGATCAAGAT GAACTGGCCTTNTCTACTTNNCAGCTTTAACAGAACACCATATTGACGATNCCAGATGAA TGGCTGGGTTATAGTAGATTCATGCCCCACTGGGATGCAA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK000336 unedited NGGGTNCAACTATGGAACCGCGGCCGCAANCTANGATCGAGTTTTTTTTTTTTTTTTTTA ACAAAATAAAAGTTTATTTTATTAGAATTAGGAATGTTCTTTCCCTCTGGGCAACCTGAA CAGTCCAGTTACAATGAAATCTAAGAACAGTTATTTCCACAGCATAGGGTGCTCTATGCA CAAAATTACAGGGTTAGGAGCATTTAATCAATTTACCATTCTGATTGTCTCACCAACCCA CCCCATCCCATCCCCCAAGTGCTCTATTACCGGGGTTTGTAGCTAAAATAATTTGTTACA TTAACTCCTTTTCCCTCTTCTACTAAATTTCAGTTCCTAATCATGGTTGAACCACTTTAT TACACAGCAGATTCAACCATTTCAAATGAAGTCAGCCAGTCTTGGATCTGTTGTATATGG GTGTATGTATACACGTGTATCTAACATATTTATTTAAACACACATAAAACACATACAATC TGGGTTGTGGAAAATTACAGATAATTTAGTCTGATATTATATGCTACTCAATTTTACCAC TGTGGTTTGTGTCCTGAGCTTTGGATCAAAATTTTATTTTAAATCACTGAAAGTCCATCT GCTGCTGAATGAATTGTTCAAATGAAGCATGCACTTTTCAAAAGACAATGAAACTGACCT CAAGACATGGAGACCTTGGTGCTTACTGTGAATAGATCCCTCAGAAAGACTTCTGTAGGT GAAACAGGGAAGCTGTCATGGCTTGCCTATCCCAGCTGGGGGCACTGAATCTACTAATAA CACAAGCAATTCACTCTGGGGATTCTGTCAATATGGGTGGTATCTGTTAAAAGCTGGGAA GTAGAAAGGCCAGTTCATCTTGATCATACAATTANGGAATGCACCCACACTGGCATACCC TCAGGCAGTATGCCACATTGAAATANAAGA
Restriction Sites	NotI-NotI
ACCN	AK000336
Insert Size	1750 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK000336.1
RefSeq Size	2995 bp
RefSeq ORF	2995 bp
Locus ID	25820
Protein Families	Druggable Genome
Gene Summary	E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3 (PubMed:15236971, PubMed:21532592, PubMed:24076655, PubMed:27565346, PubMed:23707686). Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-RING ubiquitin ligase (CRL) complexes and initiating ubiquitination of CRL substrates: associates with CRL complexes and specifically mediates addition of the first ubiquitin on CRLs targets (PubMed:27565346). The initial ubiquitin is then elongated by CDC34/UBE2R1 and UBE2R2 (PubMed:27565346). E3 ubiquitin-protein ligase activity is activated upon binding to neddylated cullin-RING ubiquitin ligase complexes (PubMed:24076655, PubMed:27565346). Plays a role in protein translation in response to DNA damage by mediating ubiquitination of EIF4E2, the consequences of EIF4E2 ubiquitination are however unclear (PubMed:25624349). According to a report, EIF4E2 ubiquitination leads to promote EIF4E2 cap-binding and protein translation arrest (PubMed:25624349). According to another report EIF4E2 ubiquitination leads to its subsequent degradation (PubMed:14623119). Acts as the ligase involved in ISGylation of EIF4E2 (PubMed:17289916).[UniProtKB/Swiss-Prot Function]

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