FNBP3 (PRPF40A) (BC029414) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	2810012K09Rik; FBP11; Fnbp3; Fnbp11; formin binding protein 3; formin binding protein 11; NY-REN-6; OTTMUSP00000013317; pre-mRNA processing factor 40 homolog A; PRP40 pre-mRNA processing factor 40 homolog A (yeast)
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC029414, the custom clone sequence may differ by one or more nucleotides ACTCAGATCGCTTCTTGTTGGTATGTGTAGCGGCAGTGGCCGCCGGCGGAGCAGTCTGAGCCCGACGATG AGGCCGGGGACGGGAGCTGAGCGTGGAGGCCTCATGATGGGGCACCCTGGCATGCATTATGCCCCAATGG GAATGCACCCTATGGGTCAGAGAGCGAATATGCCTCCTGTACCTCATGGAATGATGCCGCAGATGATGCC CCCTATGGGAGGGCCACCAATGGGACAAATGCCTGGAATGATGTCGTCAGTAATGCCTGGAATGATGATG TCTCATATGTCTCAGGCTTCCATGCAGCCTGCCTTACCGCCAGGAGTAAATAGTATGGATGTAGCAGCAG GTACAGCATCTGGTGCAAAATCAATGTGGACTGAACATAAATCACCTGATGGAAGGACTTACTACTACAA CACTGAAACCAAACAGTCTACCTGGGAGAAACCAGATGATCTTAAAACACCTGCTGAGCAACTCTTATCT AAATGCCCCTGGAAGGAATACAAATCAGATTCTGGAAAGCCTTACTATTATAATTCTCAAACAAAAGAAT CTCGCTGGGCCAAACCTAAAGAACTTGAGGATCTTGAAGGATACCAGAATACCATTGTTGCTGGAAGTCT TATTACAAAATCAAACCTGCATGCAATGATCAAAGCTGAAGAAAGCAGTAAGCAAGAAGAGTGCACCACA ACATCAACAGCCCCAGTCCCTACAACAGAAATTCCGACCACAATGAGCACCATGGCTGCTGCCGAAGCAG CAGCTGCTGTTGTTGCAGCAGCAGCAGCGGCAGCAGCAGCAGCAGCTGCAGCCAATGCTAATGCTTCCAC TTCTGCTTCTAATACTGTCAGTGGAACTGTTCCAGTTGTTCCTGAGCCTGAAGTTACTTCCATTGTTGCT ACTGTTGTAGATAATGAGAATACAGTAACTATTTCAACTGAGGAACAAGCACAACTTACTAGTACCCCTG CTATTCAGGATCAAAGTGTGGAAGTATCCAGTAATACTGGAGAAGAAACATCTAAGCAAGAAACTGTAGC TGATTTTACTCCCAAAAAAGAAGAGGAGGAGAGCCAACCAGCAAAGAAAACATACACTTGGAATACAAAG GAAGAGGCAAAGCAAGCTTTTAAAGAATTATTGAAAGAAAAGCGGGTACCATCGAATGCTTCATGGGAGC AGGCTATGAAAATGATTATTAATGATCCACGATACAGTGCTTTGGCAAAGTTAAGTGAAAAAAAGCAAGC CTTTAATGCCTATAAAGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC029414 unedited CATAATTTGTAATACGACTCACTATAGGGCGGCCGCGAAATTCGCACCAGCTACCCCTGG CGGCCTCAGGTTTGTGGGGCGTACGTAACTCGGGCGGGGAGGGTGAAGGTGGCAGACCCC CACTGCCCCACCCCCTACGGGGTGAGGCGGTCTAAGCTCGGGAAGCTGGGACACTGTTGT CTTCCCTTCCCCTGGGGAAGTTGGTAGAGTTTTCCCGACGGAGGACTCGGCGCAGAAACT TCCTCCGTCCTCCTCCTCCCCCCCCCCCATGCAGGCGACGCCGAGTGAGGCTGAGGCTGG GGGCGAATCGCCGCAGAGCTGCGTGTCGGCGGCGCATTCTGATTGGACAGCGGGGAAGCC CGTCAGCCTGTTGGCCCCGCTGATCCCGCCCCGTAGCGCAGGGCAGCCTTTAACCTTTAG CCCCAGTGGGCGCCAGCCACTCAGATCGCTTCTTGTTGGTATGTGTAGCGGCAGTGGCCG CCGGCGGAGCAGTCTGAGCCCGACGATGAGGCCGGGGACGGGAGCTGAGCGTGGAGGCCT CATGATGGGGCACCCTGGCATGCATTATGCCCCAATGGGAATGCACCCTATGGGTCAGAG AGCGAATATGCCTCCTGTACCTCATGGAATGATGCCGCAGATGATGCCCCCTATGGGANG GCCACCAATGGGACAAATGCCTGNAATGATGTCGTCAGTAATGCCTGGAATGATGATGTC TCATATGTCTCAAGCTTNCATGCAGCCTGCCTTACCGCCAGGAGTAAATAGTATGGATGT AGCAGCAGGTACAGCATCTGGTGCAAAATAATGTGGACTGAAACATAATCACCTGATGGA AGGACTTACTACTANCACACTNGAAACCAACAGTCTACCTGGGGAAAACCAGATGATCTT AAACACCTGCTGAGCACTCTTTCTTAATCCCCTGNAGGATACAATCAGATCTGGAAAGCC TACTATATATNCTCAACAAAGAATTNGCTGG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC029414 unedited GGTTCAACTATGNNACCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTCTG TCTGGACTTTATAGGCATTAAAGGCTTGCTTTTTTTCACTTAACTTTGCCAAAGCACTGT ATCGTGGATCATTAATAATCATTTTCATAGCCTGCTCCCATGAAGCATTCGATGGTACCC GCTTTTCTTTCAATAATTCTTTAAAAGCTTGCTTTGCCTCTTCCTTTGTATTCCAAGTGT ATGTTTTCTTTGCTGGTTGGCTCTCCTCCTCTTCTTTTTTGGGAGTAAAATCAGCTACAG TTTCTTGCTTAGATGTTTCTTCTCCAGTATTACTGGATACTTCCACACTTTGATCCTGAA TAGCAGGGGTACTAGTAAGTTGTGCTTGTTCCTCAGTTGAAATAGTTACTGTATTCTCAT TATCTACAACAGTAGCAACAATGGAAGTAACTTCAGGCTCAGGAACAACTGGAACAGTTC CACTGACAGTATTAGAAGCAGAAGTGGAAGCATTAGCATTGGCTGCAGCTGCTGCTGCTG CTGCCGCTGCTGCTGCTGCAACAACAGCAGCTGCTGCTTCGGCAGCAGCCATGGTGCTCA TTGTGGTCGGAATTTCTGTTGTAGGGACTGGGGCTGTTGATGTTGTGGTGCACTCTTCTT GCTTACTGCTTTCTTCAGCTTTGATCATTGCTTCAAGATCCTCAAGTTCTTTAGGTTTGG CCCAGCGAGATTCTTTTGTTTGAGAATTATAATAGTAAGGCTTTCCAGAATCTGATTTGT ATTCCTTCCAGGGGCATTTAGATAAGAGTTGCTCAGCAGGTGTTTTAAGATCATCTGGTT TCTCCCAGGTAGACTGTTTGGTTTCAGTGGTGTAGTAGTAAGTCCTTCCATCAGTGATTT ATGTNCAGTCCACATTGAC
Restriction Sites	NotI-NotI
ACCN	BC029414
Insert Size	1750 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC029414.1, AAH29414.1
RefSeq Size	1310 bp
RefSeq ORF	1310 bp
Locus ID	55660
Domains	WW, FF
Protein Pathways	Spliceosome
Gene Summary	Binds to WASL/N-WASP and suppresses its translocation from the nucleus to the cytoplasm, thereby inhibiting its cytoplasmic function (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape and migration. May play a role in cytokinesis. May be involved in pre-mRNA splicing.[UniProtKB/Swiss-Prot Function]

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