ESD (AK093643) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FGH
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK093643, the custom clone sequence may differ by one or more nucleotides GTCTATTTTTGGCCACTCCATGGGAGGTCATGGAGCTCTGATCTGTGCTTTGAAAAATCCTGGAAAATAC AAATCTGTGTCAGCATTTGCTCCAATTTGCAACCCTGTACTCTGTCCCTGGGGCAAAAAAGCCTTTAGTG GATATTTGGGAACAGATCAAAGTAAATGGAAGGCTTATGATGCTACCCACCTTGTGAAATCCTATCCAGG ATCTCAGCTGGACATACTAATTGATCAAGGGAAAGATGACCAGTTTCTTTTAGATGGACAGTTACTCCCT GATAACTTCATAGCTGCCTGTACAGAAAAGAAAATCCCCGTTGTTTTTCGATTGCAAGAGGCAAGTATTA GGAAACTTTAGCTTGATCTAGATAATGTAACTATGAAAAATTAGGATTCTGTGATATTTTCTTAACTTTA TTTTAGAATATAAAATGCTACTTCTAGTAAGTTTTTAATTATATTCAGACACTTGAAGTGAAAACCTGCC TATAAAATTAATTATATCTAACACTAAATTTGATAGAAATAGGAAAGCTTTTGGCAGTGAAAAATATTGT TTTCCATCAAACCAGTAAATAACAAGATTTTTAATGCTTGAATGATGATTAATCTTCAGCATTAGCAGAT ACTTCTTTCTTTACACATGGACCTTTGCTGCAGTAGAATAAAAAGGACAAACTTCTACCTAAATTACTCC TTATTTTATGTATATATTTTAGCCTCAGAAGTTAAGTATTATTTATCACACATGTGCTTTATATTATTTG ACTTTCATATTAATCTATTCATTGTGCTACTTGATATTTCTAGTTGTTTAAGAATAATTACTGAATCCCA ATGGGCTTGCAAGTTTTTTTTAACTAAAATATTTTTGACCGTCCACTACTCACCTAACTCTATATATGCT ACCATAGTCCTAATTAAGGAAGTTCTGCTTTTATTTTTAAGAAAAATTCATAAGCTTCAGAATATAATTT AAAATATTTTAGAGTGAAAAATGAGCACTGAAAATTATACATGGTCATGATTTTTAAATAAATTTTTATC CATGTTTAAGGATAATGTGTCAGGCACATTGATCTAAAATGTAATTACAATGGTAAATGTTCAAATCTAG ATCAGTGTGTAATGTGGTACATTAGTTTGTAAAATGCTTTCACAGCATTAACTCATTGTTAATTCTTATA CCGCCAAATGAGATATCATTTCTTTCCTATCCATCTGTTTTACAAATGAGGAGACAGGCTCAGAGAAGTA ACCTACTTATCTAAGGCCACATAGCTAGTAAGTGGTGGAACCAGAACTGGAACTAAGGCCATTTAACTGC AAATACTGACCTCCTGTCATTTATCAAGCCATTTTCAGACGGCATGATGTTCTCCTGAGGAGCAAAGGAA TGGAATTAGAGCTAGCACAAGGAGAAATTGTGCATAGCTTGCTGCCTACGTAACTTATGCAGAAGATCTG AGCAGTCTTCCTGTTTTTAGGGATATTTTGCACATGTGAACTATATAGTGAGAAAATGTCAAAAACTAAT ACATGATATACTGTTTGTCTCTTAGTGTCCATCCTTGAGTAATAGAATTTTGTTTTAGATTTATTTGAAC ATTTACCATCACAATTAAATTTTAGGTCAACAACCAGCCTTGAGAAATCCAAATACCAATTTGACTATAA ATATAGTAATACGTTTAACTGCAATAACTATAGCTATAAGTGCGTACCAAACCATGTTTTTTCCAAATGA CCTTAATTTCTTGGCACTACCCATGTTGGATACCTCATTTGCTTCCCCAAAGACTGTGCCCAAAATTTCT GTTGTTCCTACAATGATTCTGCCTGCCAACAAAGTCTGAAAATCTCTCAAGTTTGTTATAAAAACAGCAC AGTTAGAACATGATGACATTCCTGGAGGGAAAAATAAGTCCACTTATTTGTTTCTAAAATGCCTGTATCC CAAGCAACTAGAACACATAGTTGGCTATACTTAATGAGGAGTCAAGACAAACCATTCAATTCTTCTAAAA AAGTAAGAAAGTATTCAAAGCACCAGAGTCACAATGTTGAGCTCAGGTGCAATTTTCTATTCACTTTACT TCCAGCATGGTCGGGTTAAGAGTGGACTACATAGCTTGAAGCGGATTTCGTTGAGATTCGTTGTAATTGA ATCAACAACTGAATGAGCATGCCTAAGTAGTGGCTCCAGAGAGTCTGCTTTCAGATTTTCTTGCCAGCTT TGACCCAAGATTACCCTGGGTCTGCGAGTTTTATCACCTGCTTTATCATTTGATTACAAGTATAGTATAA CTGTATTTCCTTGTGGGATAGAAGCTGTAAAATAAGCTGAAAATAATAGGGGATATATATAAATAAACGA TCTGAGAAAGAATAAAGAAAATAATTAC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK093643 unedited CGGCCGCGAATTCGGCACGAGCTATTTTTGGCCACTCCATGGGAGGTCATGGAGCTCTGA TCTGTGCTTTGAAAAATCCTGGAAAATACAAATCTGTGTCAGCATTTGCTCCAATTTGCA ACCCTGTACTCTGTCCCTGGGGCAAAAAAGCCTTTAGTGGATATTTGGGAACAGATCAAA GTAAATGGAAGGCTTATGATGCTACCCACCTTGTGAAATCCTATCCAGGATCTCAGCTGG ACATACTAATTGATCAAGGGAAAGATGACCAGTTTCTTTTAGATGGACAGTTACTCCCTG ATAACTTCATAGCTGCCTGTACAGAAAAGAAAATCCCCGTTGTTTTTCGATTGCAAGAGG CAAGTATTAGGAAACTTTAGCTTGATCTAGATAATGTAACTATGAAAAATTAGGATTCTG TGATATTTTCTTAACTTTATTTTAGAATATAAAATGCTACTTCTAGTAAGTTTTTAATTA TATTCAGACACTTGAAGTGAAAACCTGCCTATAAAATTAATTATATCTAACACTAAATTT GATAGAAATAGGAAAGCTTTTGGCAGTGAAAAATATTGTTTTCCATCAAACCAGTAAATA ACAAGATTTTTAATGCTTGAATGATGATTAATCTTCAGCATTAGCAGATACTTCTTTCTT TACACATGGACCTTTGCTGCAGTAGAATAAAAGGACAAACTTCTACCTAAATTACTCCTT ATTNTATGTATATATTNTAGN
Restriction Sites	NotI-NotI
ACCN	AK093643
Insert Size	6000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK093643.1
RefSeq Size	2408 bp
RefSeq ORF	2408 bp
Locus ID	2098
Gene Summary	This gene encodes a serine hydrolase that belongs to the esterase D family. The encoded enzyme is active toward numerous substrates including O-acetylated sialic acids, and it may be involved in the recycling of sialic acids. This gene is used as a genetic marker for retinoblastoma and Wilson's disease. [provided by RefSeq, Feb 2009]

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