SLC39A4 (AK056900) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AEZ; AWMS2; ZIP4
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK056900, the custom clone sequence may differ by one or more nucleotides ACTCAAGGCTCCTCCCAGGTGGCGGAGGAGAGCCCGGAGCTGCTGAACCCTGAGCCCAGGAGACTGAGCC CAGAGTTGAGGCTACTGCCCTATATGATCACTCTGGGCGACGCCGTGCACAACTTCGCCGACGGGCTGGC CGTGGGCGCCGCCTTCGCGTCCTCCTGGAAGACCGGGCTGGCCACCTCGCTGGCCGTGTTCTGCCACGAG TTGCCACACGAGCTGGGGGACTTCGCCGCCTTGCTGCACGCGGGGCTGTCCGTGCGCCAAGCACTGCTGC TGAACCTGGCCTCCGCGCTCACGGCCTTCGCTGGTCTCTACGTGGCACTCGCGGTTGGAGTCAGCGAGGA GAGCGAGGCCTGGATCCTGGCAGTGGCCACCGGCCTGTTCCTCTACGTAGCACTCTGCGACATGGTCAGG ATGGCGAGGGGAGGGGCTGCTCTGGGCCGGGAGCTGAGCAGAGGAGCTGAGCAGGGGCGCTGACCCGGTG CCCACTTGCTCCTCAGCTCCCGGCGATGTTGAAAGTACGGGACCCGCGGCCCTGGCTCCTCTTCCTGCTG CACAACGTGGGCCTGCTGGGCGGCTGGACCGTCCTGCTGCTGCTGTCCCTGTACGAGGATGACATCACCT TCTGATACCCTGCCCTAGTCCCCCACCTTTGACTTAAGATCCCACACCTCACAAACCTACAGCCCAGAAA CCAGAAGCCCCTATAGAGGCCCCAGTCCCAACTCCAGTAAAGACACTCTTGTCCTTGGAGCATGGCTGTG CTCTCCTTGCTGGGTGGGAAGGGCCCTCAGCTGAGAAAGGACAATTGCGGCACTGCCTTCTCCCCATCGG GGTGCCTCGAAGCCAGAGAGCTGCACTCAGGACCCCCAGGCAGCCAGCCTCAGGTGCCCCCTCTTTGATA TGGAGACCCTGCCTGCCTGAGGAGTGGGGTCAGGGAAATTCACCCGGGTGTGTGGGCAGCAGGTGGGACC TGTCCCCAAACAGGAATCAAACTTGCTTACCTATAACTGCTTACACAGCCGAGGGCTTATTTCTCTGTCA TGGAAAGCAAACCTGGAGATCGTCAGACCAGAGTGTGTGGCAGCCCACGGTTCCTGGGACGCCAGCCTCT TCTGACTCTGAGGCGTTAATCCTGAGTGCCTGTGTGGTGGCCTCCTAGGGCTACCTTAACAAAGCCTACC ATAAACTGAGTGCCTTAAAACCAGAAACTTATTCTCACAGTTCTGGAGGCTGAAAGTTAGGAATCAAAGT GTCAGGAGAGCAATGCTCTCTCTGAAGTCTCTAGGAAACAGTCTGTTCCATGAGCCTTTCCTGGCTACTG GTGGCCCCAGGTGTTCCTTGGTTTGTGGGCAGTATGACTCCAATCCCTGCCTTTGTCTTCACATGACCTT CCCTCCCCTCCCCTCCCCTTTTCTTTTCTTTCTTCTTTTGAGACAGAGCTTCACTCTGTCGCCCCGGCTG GAGTACAGTGGCACCATCTCGGCTCACTGCAACCTCTGCCTCCCAGGTTCAAGCAATTCTGCATCAGCCT CCCGAGTAGCTGGGATTACAGGCACCCGCCACCACGCCCAGTTAATTTTTATATTTTTAGTAGGACGGGG TTTCACCATGTTGGCCAGGCTGGTCTCGATCTCCTGACCTCAGGTAATCCACCCACCTCGATCTCCCAAA GTGCTAGGATTACAGGTGTGAGCCATTGCGCCTGGCCCACATGACCTTCTTTCTTGTGTTTCTGTCTCTT CTGATTATTTTTATTGTATGTGACTCTTGTCACTCAGGCTGGAGTGCAATGACGCCATCTTGGCTCACTG CAACCTCTGCCTCCTGGGTTCAAGTGATTCTCCTGCCTCAGTCTCCTGAGTAGCTGGGATTACAGGCATG CGCCACCACCCTGGCTAATTTTTGAAGGGAGCCAGCCCCTCCACACCTGTGAGTATTTCTCATCAGGTGG GACGAGAGACTGAGAAAAGAAGACACACAGACGAAGTATAGGGAAAGAACAGTGGGCCCAGAGGACCCAG CATACGGAGGACCTGCATCAGCACCGGCCTCTGAGTTCCCACAGTATTGATCATTATTTTTACCATCTTA GCGAGGGGAGTGTAGCAGGGCAACAAGTGGGGAGAAGGTCAGCGCGGAAACATGTGAGCAAAGGAATCTG TATCATGAATAAGTTCAAGGAAAGGTACTGTGCCCGGATGTGCACGTAGGCTAAATTTATGTTTCTCTTT ACCCAAACATGTCATTGTAGCAAAGAGTAACAGAGCAGCAGCGCTAGATTTATGTTTCTCTTTACCCAAA CATCTCAGTGTAGGAAAGAGTAACAGAGCAGTATTGCTGCCAGCATTCTTGCTTCCAGGCAGTTTTCTCC TATCTCAGAATAGAACAAAAGGGAATGGTTGGCTTTACAATGAGACATTCCATTCCCAAAGACGAGCAGG AGACAGAAGGCTTCCTCTTATCTCAACTGCAAAGAGGCCTCCCTCTTTACGACTCCTCAGCACAGACCCT TTATGGGTGTTGGGCTGGGGGACGGTAAGGTCTTTCCTTTCCCATGAGGCCATATCTCAGGCTGTCTCAG TGGGGGGAAACCTTGGACAATTCCCAGGCTTTCTTGGGCAGAGGTCCCTGCGGCTTCCCGCAGTGCATTG TGTCCCTGGTTAATCGAGAATGAAGAATGGCGATAACTTTTACCAAGCATACTGCCTACAAACATATTAA CAAGGCACATCCTGCATAGCCCTAAATCCATTAAACTTTGATTCATTAC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK056900 unedited CCGCGAATTCGGCACGAGGCTGAACCCTGAGCCCAGGAGACTGAGCCCAGAGTTGAGGCT ACTGCCCTATATGATCACTCTGGGCGACGCCGTGCACAACTTCGCCGACGGGCTGGCCGT GGGCGCCGCCTTCGCGTCCTCCTGGAAGACCGGGCTGGCCACCTCGCTGGCCGTGTTCTG CCACGAGTTGCCACACGAGCTGGGGGACTTCGCCGCCTTGCTGCACGCGGGGCTGTCCGT GCGCCAAGCACTGCTGCTGAACCTGGCCTCCGCGCTCACGGCCTTCGCTGGTCTCTACGT GGCACTCGCGGTTGGAGTCAGCGAGGAGAGCGAGGCCTGGATCCTGGCAGTGGCCACCGG CCTGTTCCTCTACGTAGCACTCTGCGACATGCTCCCGGCGATGTTGAAAGTACGGGACCC GCGGCCCTGGCTCCTCTTCCTGCTGCACAACGTGGGCCTGCTGGGCGGCTGGACCGTCCT GCTGCTGCTGTCCCTGTACGAAGATGACATNACCTTCTGATACCCCTGCCTAGTCCCCCA CCTTTGACTTAAGAATCCACACCTTACAAACCTACGGCCCAGAAACCGAAGCCCCCATAG AAGGGCCCAGTCCCAAACTCCGTAAAGACCCTCTTGTCTTGGGAAAAAAAAAAAAAAAAC CCCGACCTCTAATTGGGGCCCGGGCCTAGCCTTTTCTGGACACAACCCCGGGGGATCCCT GGACCCCCTCCCAATGCTTTA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK056900 unedited GGGGAGGGGGGNNNNNNNNNNNNNNGNTTTNNNNNNNNTATTGACTNTGNNACGNCGNCC GCTAACTAGNGATCGATTTTTTTTTTTTTTTTTTTCCAGGGCAAGNATGTCTTTACTGGA GTTGGGACTGGGGCCTCTATAGGGGCTTCTGGTTTCTGGGCTGTAGGTTTGTGAGGTGTG GGATCTTAAGTCAAAGGTGGGGGACTAGGGCAGGGTATCAAAAGGTGATGTCATCCTCGT ACAGGGACAGCAGCAGCAGGACGGTCCAGCCGCCCAGCATGCCCACGTTGTGCATCAGGA ATAGGATCCAGGCCGCGGGTCCCGTACTTTCACATCGCCGGGAGCATGTCGCAAAATGCT ACTTAAAAGACCAAGGCCGGTGCCACTTGCAGGAATCAAGGCTTGGTTTTCATGGCTGAC TCCAAACCCCGAATGCACCTTTAAAACCAAACAAAGGCCTTTAACCCCGAAAGACAAATT TAACCCCAATGCTTTTGGCCCAGAAAACCCCCCCGTTTTAAAGGGGGGGAAAATCCCCCC CCCTTGGGGAAAATTTGGGGAAAAAACGGCCCCCGAGGGTGGCCCCCCCCGCCTTCCCCG AAGAACCCCAAGAGGGGGCCCCCAGGGCAAACCCCTTGGCGAAATTTTGGCCACGCGTCC CCCCCCAATGTTTTCTAATGGGGGGGAAACCCCCCCCTTGGGGGGCTCACTTCTCGGGGG GCAAGGGTTATCCCCCGGGGCGCAAAAAATTGGGCCCCCCCCCTTTAATGGTGTTGGTTA AAAAAAAATTTTCGGGGGGTTCAAAAAAAAAACCTCTCTTTTTTATAACCCCCCCCCCCA AAAACCCCCCCCCCCCCTTTTTTTTTTTAAGGGGGGGGT
Restriction Sites	NotI-NotI
ACCN	AK056900
Insert Size	700 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK056900.1
RefSeq Size	2779 bp
RefSeq ORF	2779 bp
Locus ID	55630
Domains	Zip
Protein Families	Transmembrane
Gene Summary	This gene encodes a member of the zinc/iron-regulated transporter-like protein (ZIP) family. The encoded protein localizes to cell membranes and is required for zinc uptake in the intestine. Mutations in this gene result in acrodermatitis enteropathica. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2013]

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