UBE2D3 (AK095822) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	E2(17)KB3; UBC4/5; UBCH5C
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK095822, the custom clone sequence may differ by one or more nucleotides ATGGCGCTGAAACGGATTAATAAGGAACTTAGTGATTTGGCCCGTGACCCTCCAGCACAATGTTCTGCAG GTCCAGTTGGGGATGATATGTTTCATTGGCAAGCCACAATTATGGGACCTAATGACAGCCCATATCAAGG CGGTGTATTCTTTTTGACAATTCATTTTCCTACAGACTACCCCTTCAAACCACCTAAGGTTGCATTTACA ACAAGAATTTATCATCCAAATATTAACAGTAATGGCAGCATTTGTCTCGATATTCTAAGATCACAGTGGT CGCCTGCTTTAACAATTTCTAAAGTTCTTTTATCCATTTGTTCACTGCTATGTGATCCAAACCCAGATGA CCCCCTAGTGCCAGAGATTGCACGGATCTATAAAACAGACAGAGATAAGTACAATAGGTTAGCAAGAGAG TGGACAGAGAAATACGCTATGTTGTAG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK095822 unedited AATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCCACCCGGGTC TCTTCCTTTCTAGAGGCCGGGAAGTTAAACTTGTAGCCACCACCTCCGCTCTTCCCGTCA CCCTCGCCCCCACTTCGGGCCGAAAGCACGGTACAGAGGCTGTTGGTGGCTTTGCCACGC CACCCCACCCACCCCGGATCGCGGCTGTCTTAAGGGACCTGGATTCATCAGGGGCTCTTC GGGGCCTGTGCGAGTGCTGATCTGCTCCGTTTTTGCAAAAGGCGCCTGTGTCTGGCAGAG CTGGTGTGAGACGAGACAATCCTGCCCCGCCGCCGGGATAATCAAGAGTTTTGGCCGGAC CTTTGAGCATACACCGAGAGAGTGAGGAGCCAGACGACAAGCACACACTATGGCGCTGAA ACGGATTAATAAGGAACTTAGTGATTTGGCCCGTGACCCTCCAGCACAATGTTCTGCAGG TCCAGTTGGGGATGATATGTTTCATTGGCAAGCCACAATTATGGGACCTAATGACAGCCC ATATCAAGGCGGTGTATTCTTTTTGACAATTCATTNTCCTACAGACTACTCCTTCAAACC ACCTAAGGTTGCATTTACAACAAGAATTTATCATCCAAATATTTAACAGTAATGGCAGCA TTTGTCTCGATATTCTAAGATCACAGTGGNTCGCCTGCTTTAACAATTTCTAAAGTTCTT TTATCCATTTGTTCACTGCTATGTGATCCAAACCCAGATGACCCCCTAGTGCCAGAGAAT GCACGGGATCTATAAAACAGACCGAGTATAGTCCACCAGAATATCTTCGGAAATGGACTC ANAAATATGCCATGGGATGCTACCTTAAGGCCAGAATACCTGCATTATACTG
Restriction Sites	NotI-NotI
ACCN	AK095822
Insert Size	1550 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK095822.1, BAC04632.1
RefSeq Size	2365 bp
RefSeq ORF	447 bp
Locus ID	7323
Domains	UBCc
Protein Pathways	Ubiquitin mediated proteolysis
Gene Summary	The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. [provided by RefSeq, Jan 2017]

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