NDUFV3 (BC033766) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CI-9KD; CI-10k
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC102290 sequence for BC033766 edited (data generated by NextGen Sequencing) ATGGCTGCCCCGTGTTTGCTGCGGCAAGGACGAGCCGGGGCGCTGAAGACTATGCTCCAG GAAGCCCAGGTGTTTCGAGGACTTGCTTCTACGGTTTCTTTGTCTGCGGAATCAGGGAAG AGTGAAAAGGGTCAGCCACAGAATTCCAAGAAGCAAAGTCCACCAAAAAAGCCAGCCCCA GTGCCTGCTGAGCCGTTTGACAACACTACCTACAAGAACCTGCAGCATCATGACTACAGC ACGTACACCTTCTTAGACCTCAACCTCGAACTCTCAAAATTCAGGATGCCTCAGCCCTCC TCAGGCCGGGAGTCACCTCGACACTGA Clone variation with respect to BC033766.1 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC033766 unedited GTTCACATTAGGNAATACGACTTACTATTAGGGCGGCCGCGAATTCGCACGAGGGTCACC GCCATGGCTGCCCCGTGTTTGCTGCGGCAAGGACGAGCCGGGGCGCTGAAGACTATGCTC CAGGAAGCCCAGGTGTTTCGAGGACTTGCTTCTACGGTTTCTTTGTCTGCGGAATCAGGG AAGAGTGAAAAGGGTCAGCCACAGAATTCCAAGAAGCAAAGTCCACCAAAAAAGCCAGCC CCAGTGCCTGCTGAGCCGTTTGACAACACTACCTACAAGAACCTGCAGCATCATGACTAC AGCACGTACACCTTCTTAGACCTCAACCTCGAACTCTCAAAATTCAGGATGCCTCAGCCC TCCTCAGGCCGGGAGTCACCTCGACACTGAGGGCCCTCGGTGTGAAGATGAACCTTCCAC CGTCTTCACTGCATCCTGGAGTGCAAAAATAAAATCCACTCAAGAGTCAAAAAAAAAAAA AAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGT GGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGC CCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCT ATAATATTATGGGGTGGAGGGGGGTGGTATGGAGCAGGNGCAAGTTGGGAAGACACCTGT ANGGCTGCGGCTATGACCACTGTGNGCCTCCTCNTGNGNNGTTCTTTTTGNGAACAACCA NAGTTGTGGAGGTGCAGGGGAGCACAAATTTGTGTCTACACGTGCAAATCTCCCCGCCTC CCTGGTGTTACAAGACGATTTCTTCCTCGCCTACACCCCTCCCCGAGTTTGTGTGGGGAT CTCCAGGNCTATTGCATTGACCCANGGCTACAGCTTTAATACTGTTGTTTTTTTTGGNNT AGAGCACGGGGNGTTTTAACAATTATTGGGCCGANGGCTGGGNTCTTCCAACTNCCTAAA TNCTCGAGGTGGGATCTAACCCCAACCTTTGCNCTTCCCAAAATATGCTTGGGAAT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC033766 unedited AATTCAGTGTGNACGNCCGCCGTATGCTAGNGATCGGTTTTTTTTTTTTTTTTTTGACTC TTGAGTGGATTTTATTTTTGCACTCCAGGATGCAGTGAAGACGGTGGAAGGTTCATCTTC ACACCGAGGGCCCTCAGTGTCGAGGTGACTCCCGGCCTGAGGAGGGCTGAGGCATCCTGA ATTTTGAGAGTTCGAGGTTGAGGTCTAAGAAGGTGTACGTGCTGTAGTCATGATGCTGCA GGTTCTTGTAGGTAGTGTTGTCAAACGGCTCAGCAGGCACTGGGGCTGGCTTTTTTGGTG GACTTTGCTTCTTGGAATTCTGTGGCTGACCCTTTTCACTCTTCCCTGATTCCGCAGACA AAGAAACCGTAGAAGCAAGTCCTCGAAACACCTGGGCTTCCTGGAGCATAGTCTTCAGCG CCCCGGCTCGTCCTTGCCGCAGCAAACACGGGGCAGCCATGGCGGTGACCCTCGTGCCGA ATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCT CTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATTTGCGTCAACGGGGCGGGG TTATTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCCAAAACAAACTCCCATTGACG TCAATGGGGTGGAGACTTGGAAATCCCCGTGAGTCAAACCGCTATCCACGCCCATTGGTG TACTGCCAAAACGCATCACCATGGTAATAGCGATGACTAATACGTAGATGTACTGCCCAG TAGGAAAGTCCCGAAGGTCATGTACTGNGCATAATGGCAGGCGGGCCATTTACCGTCATT GACGTCAATAGGGGGCCGGACTGGCATATGATACACTTGATGTACTGCCCAATGGGCCGA
Restriction Sites	NotI-NotI
ACCN	BC033766
Insert Size	430 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC033766.1, AAH33766.1
RefSeq Size	1050 bp
RefSeq ORF	1050 bp
Locus ID	4731
Protein Pathways	Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
Gene Summary	The protein encoded by this gene is one of at least forty-one subunits that make up the NADH-ubiquinone oxidoreductase complex. This complex is part of the mitochondrial respiratory chain and serves to catalyze the rotenone-sensitive oxidation of NADH and the reduction of ubiquinone. The encoded protein is one of three proteins found in the flavoprotein fraction of the complex. The specific function of the encoded protein is unknown. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

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