SIRT5 (BC035196) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	SIR2L5
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC035196, the custom clone sequence may differ by one or more nucleotides AGCGGGATGTGACCCACAGCCCTATTGGCTCAGCATGCGAGAGAGCAGGGATGGTGCTCCCCAAGGAAGG ACTGGTGGGCAGGACGGAGCAGCGCATGTCCACTAGGGTTGAAAGTGCTCTGCACGGCACCAGTTGCCTG GTACCCATTTCATAAACTGTGGCCACCATGATTGCTTCAGCTCAATAAGTGGTGGTTAGTATTAACAATT ACTTTTTTCACTTATTAGTTGATTACTTATTTATTGAGGTGCTTTTGTATAAAAGATTCAAGCTTTCTGA AATCGAAGTTAAAATTTTGTAAACATATCCCACGCCATTGAACCTTTGTCAGTCATGTCAATAAATCACG CCCTTAGATTTAGGTTGAGCTCCCTCTACCCTATTGGCAACTTCTGCCCACTTGAACACCACTCCAAGGT CATCTGATGGCACATTAGTCATGGAGTTATTTATTTTTCCTCCAGATTATAAAGTCCTTGAGGGCATATG CTCTTGACTGGCTTAAGAGTACTGCATGCCTCTGTTTCCCATGAAAGCTAAAGCTAGGGTAGGCCCATTT GCTAAAATGAAGTATGGCTCAGTGTGGCGGTGCACACCTATAGTCCCAGCTGCTCAGGAGGCAAAGGTGG GAGGATCGCTTGAGCCCATGAGTTGGAAGCTGCAGTGAGCTATGATCGTACTGCTGCACTCCAGCCTGGG TAACTGGGTGAGACCAAAACATGAAATGAAGTGTGAAAAATAAAGTATCTTATACCCTTTTAGACATGGA GAAGGTTGCTCTTGATTATACTAAATTCTGGATTTGCTTCATATGTATTTTTCAGGTAGCTTATTTAAAA CTCGATGTACCTCTTGTGGAGTTGTGGCTGAGAATTACAAGAGTCCAATTTGTCCAGCTTTATCAGGAAA AGGGTAATTATACCACACTACAGAATAAGTATAGGTTGTTTTCTCCTTTAGGTTGAACATAAATGTGAGG AAATTGAACATTCATCAAAGATTCCCATGGATATTTATGAGAAATAAGACTAAATACAAGGCTGGGTGCA GTGGCTCATGCCTGTAATCCCAGCACTTTGGGGTTACAAGGTGGGGAGACTGCTTGAGTTCAGGAGTTCA AGACCAGCCTGGGCAACACAGTGAGAAACCTGTCCTACAAAAAAACAAAACAAAACAAAAAACCACAAAA ATTAGCTGGGTGTGGTGGCACATGCCTGTAGTCCCAGCTACTAGGGAGGCTGAGGTGGGAGGGTTATTTG AGCCTGGGAAGTCAAGGCTGCAGTGAGCTGAGATCATGCCACTGCACTACAGCCTGGGTGACAGAGGGAG ACCATGTCTCAAAAAACAAACAAACAAACAAAAAAACTAAATATAGCCATGTGATAAAAGAAATATTTTG CTCACAACTTACAGTGTTAAGTGAAAACCACAGGGTCCTAAATTTGTACACACAATGAGTTCGTGTATCT GTATATAGTTAAAAGACCAGAAGAAAATACAACAAAATGTTAATAAGGAATATCAGGAGTGGAGGGATTA TGGGTAATTTTTAATTTCTTCTTTATATCTTTCTGAATTTTCCTATATTTCTATTATGAACATGTACTAT TATTACAATGAGGAAAAAATTTGAGGGTAGACACAATATTGAGAAAAATTCCACTCAGCCTTTTAAAAAC AGTATAAATTGTATAAGGACTATATAATGTCATTGCAGAGGATACAAATGATGATGATGATGATGTATAA AGGTAAACATGAAGTTCCCCCTTGGCACACCCAGCCACTCCAGCCCTCCCCACACATGCATTCCCTCACT GGCAGTACAACCACTGGTGATTTTTTTTTTTCTGAGACAGGTTCTTGCTCTGTTTCCCAGGCTGGAGTGC AGTGGCACAATCACACCTTACTGCAGCCTCCAACTCCTGGGCTCGAGCAATCCTCCTGCCTCAGCCTCCC AAGTAGCTAGGACTATAGGCACACATCACCACACTCAGCTAATTTTTGTATTTCTGGTAGAAATGCAGTC TTACTGTATTGCCGAGGGTTGTCTTGAACTTCTGGCCTCAAGCAGTCCTCCCACTTTGGCCTCACCAGAT GCTGGGATTACAGGCATGAGCCACTGTGCCCAGCACCACTGGTAATTAATGGCTTGGGGTCCTTCCTTCT AATGCCCTTCCAAGTGAGCATGTATTAATTAGGAGTTTTATACCACATACTGCCAGGCAAATATACAAAC TGAGTTATGTTGTTTCTTTATTTTTTATTATGCCAATAACAATCTTTCTTTTCTAATATTATTTACTTCA GTGCTCCAGAACCTGGAACTCAAGATGCCAGCATCCCAGTTGAGAAACTTCCCCGGTAGGTAGAAAACTC TGATTTGTACTGGTGTTCCAGGTTACCAAAACAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC035196 unedited NAGGGTTCACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCTTCTT TCAGCTTTTCTCACTCTTTGAGGTGGCCTCCCTTCTCTCTCTGCAATTGATCTGTCTCCA CGCGCCAAGGGAGAGTGCCTCCGACGGCTCCAGCTCAGCCACCTGCTCAGAAAATAAACC TCTGGGTAGGCTTGCTTGGTCCACACGCCCACCCTTCTGACCAAACACGACTGCAGAGGG ATGGACACCACATGGGGGCCGTGCGCAGCATGTGGCTGAGCTTGTATTTGGGATGTGACC CACAGCCCTATTGGCTCAGCATGCGAGAGAGCAGGGATGGTGCTCCCCAAGGAAGGACTG GTGGGCAGGACGGAGCAGCGCATGTCCACTAGGGTTGAAAGTGCTCTGCACGGCACCAGT TGCCTGGTACCCATTTCATAAACTGTGGCCACCATGATTGCTTCAGCTCAATAAGTGGTG GTTAGTATTAACAATTACTTTTTTCACTTATTAGTTGATTACTTATTTATTGAGGTGCTT TTGTATAAAAGATTCAAGCTTTCTGAAATCGAAGTTAAAATTTTGTAAACATATCCCACG CCATTGAACCTTTGTCAGTCATGTCAATAAATCACGCCCTTAGATTTAGGTTGAGCTCCC TCTACCCTATTGGCAACTTCTGCCCACTTGAACACCACTCCAAGGTCATCTGATGGCACA TTAGTCATGGAGTTATTTATTTTTCCTCCAGATTATAAAGTCCTTGNAGGCATATGCTCT TGACTGGCTTAAGATACTGCATGCCTCTNGTTCCCCATGAAGCTAAAGCTAGGGTAGGCC CATTTGCTAAAATGAAGTATGGCTCAGTGTGGCAGTGCACACCTATAGTCCCAGCTGCTC AGGAGGGGCAAGTGNNGAGATC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC035196 unedited ATTTACTGTGNACCGCGCGCCGTTATCTANGATCGAGTTTTTTTTTTTTTTTTTTTTTAA TCTATGAACATTCTGTTTGGTCCTGTAACCTCTCCTATTCAGGTCCTTTCATTGCCTCCC TAACCCTGGATTAATAATGCCAGTGATGTTATTTGACCAGTTACAGGTCAGCCCCTATGC TTTCTGTACACTGGGTAACCACCTGTCGCACGACAGCTTCAGAAACTTTCCCGACCTCAA ATGATCCTACCACAACACAAAGCAAAAAGAGGTTGGAAAAAAATCATTCTTTTCATCCTA ATTTGGTTCATCCTTTGTCCTTTCACTTTTGGTCTCAGAGATCAAGCCTGGTTTTTACAT TTGTGCATTTGATTTAAGAGGCGTCTTGCCAAATACATGTTTAGGTTTTGATGTCTGTTT TTTTTTTTGTTTTGGTAACCTGGAACACCAGTACAAATCAGAGTTTTCTACCTACCGGGG AAGTTTCTCAACTGGGATGCTGGCATCTTGAGTTCCAGGTTCTGGAGCACTGAAGTAAAT AATATTAAAAAAGAAAGATTGTTATTGGCATAATTAAAAATAAAGAAACAACCTAACTTC ATTTGTATATTTTCCTGGGAGTATGTGGGATAAAACTCCTAATTAAACATGCTCACTTGG AAGGGCATTATAAGGGAAGGACCCCACGCCACTAATTACCCGTGGGTGCTGGGCACAAAT GGGCTCATGCCTGGTAATTCCCCCCTTTTGGTGAGGCCACAATGGGGAGGACTTGCCTGG AGCCACAAATTTCAAAAAAACCCCTCGCCAAATCAGTAAAATTGGCTTTTTCACCCGAAA AACAAAAATTAGCTGAATGCGGCGAAGGTGGCCCCAAAGGCCAAATACCCTGGTAGACCG ACAGCAGAAGATATCTTCCCGCCCCCAATTGTGT
Restriction Sites	NotI-NotI
ACCN	BC035196
Insert Size	3200 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC035196.1
RefSeq Size	2428 bp
RefSeq ORF	2428 bp
Locus ID	23408
Protein Families	Druggable Genome, Transcription Factors
Gene Summary	This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2010]

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