STAP2 (AK093701) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK093701, the custom clone sequence may differ by one or more nucleotides GTTTGATTAATTTGCTGGGGGGTGCTCACAGAACTCAGGGAAACACATGTTTACATTTACCAGTTTGCTC CAAGGATATTGCAAAGGACACAGATGAAGGGCTGCGTGGGGCAGGGCATGGGGAAGATGTGGAGTCCTGT GCCCTCCCTGGGCACCCCCCTCCAAGAGCCTCTGCATGTTCCGCCATCCAGAAGCTCCCTGAACCCTGTC CTCTTGGATTTTTATGGGCGCTTCTTGATGTCAGCTTTCTTCCCCGCTGGTGGGGATGGGGCCTTCTCTG AGGAGGATCGTATGAGCAACAGGAAAGGCTGGAGGAGAGAGATGTTTTGCCTGGCAGCTGAAAGGCGGAC AGAAAGGACAGGGGAAGGTTGGGGAAGTTCTGTTTCCTGAGGCCTGACGCACCCGACCTTCTAACAGAAG ACTGTAACAGGGCTGTGGGGTACACAAGCCAGGTGCCGTGGAGGAAAGCCTGTAGATAGATCCACATCAC GGCACCACCACTTCTCCCCGTGGTTCACAGGCCCTCCCCAAGCATCCCAGGGCCCTCTGCTGTGAAGGCG GCTCCCCCTGCCTCGCTACCTGGGCCCCCGCTCCCCTGTGCAGTGTGCTTATTTCAGTGCTGGTCTGACT TGCTCTTCGTGGTGGTAAGCTCCATGAAGGCAGGACCATGCTCAGGGAGTTTCCTGTCCTTCACTGCCCC AGGGATGTGGCAAATACTTAATCAGTGTTTGCCTATGGAGTGAACAGGTCCATGAATGGTCTTGGAAAAT GATGTATCCCAGCACCATGGTGGTGGTCATTCATACGTGTTCTCATGACTTCTTTGCTTTTCCTAATGGT GAGTTCGGTAGGCCAATTCATTTCTTGCTGAAAAGTGTGGTAAAGGCAAGTCTCCTAACTGGACCAAGAC TACAGCCTGCAGAACGCGCAGGGCCTGGGCATGGGGAAGCACGGATGAGGAGCCTGCTGCTGATGCTGAG CAGGAGCCGTGGAGGGGTCTACACCACTCTGCTGACTCTGCGTGATGAGGATTCAAAATGCCATTCACGC AAAACAAATTTAGCAACTAAGAAGTAGAGCGGCGCGCTGCTTTAGAAGCACTATGTGTTAGCAGGTGTGG TACACACCTGACTTTGCTTCTGTGGCTGAGGAAGGGGAATGAGTCTTCTGTGCGTAAATCTTTCCACAAG CACCATCCTGGGTGTCCTGCCTGACCAGACTTGTGAGCCAGGGCCAGGTTGAAGCATGTGCCCAGGTGAA AATTATGCATTATTTATTCATGTGAGCAACTCTGCGCCTATTTTGAAACATTCACTGCTTTGTTTTGTTT GTTTTGTCTAATTGCATGAAAGATGTGGTAGGCAGAATAATGCCCCCCAACAAAAAGTGTCCACTTCTTA ATCCCTGGAACCTGTGAATATGTTACCTTACTAGCAAAAGGGACTTTACAGATATGACTAAGGCTAAGGA CCTTGAGTTGCAGAAATTATCCTGGACCATCCAGGCAGGCCTGGAGACATGAGTCCTCTAAAAACAGAGA CTCTTTCCCAGCTGTGCTCAGAACCAGAGGTGACCACCAAAGAGAGATCAGAGCCATGCCATGTGAAAAG GACTTGACTTGCCATTGCTGGCTTTCCAGATGATGAAGGGGCCTCAAGCCAAGGAATGTGGGCAGCTTCT AGAAGATGGAAAAGGCAAACAAATATTCTCCCCTAGAGCTCTGGGAAGGAACACAGCTCTGCCACCTCCT TGATTTTCACCCAGTGAAATTAGTTTTGGATTCCCAACCTATAGAACTGTAAAGTAACAAATTTGTGTTT TAAGGCCAGGTGTGGTGGCTCACGCCTGTAATCTCAGCACTTTGGTTTTGGAAGGCCGAGGTGGGAGAAT CATTTGAGGCCAGGAGTTCGAAACCAGCTTGGGCAACATAGTGAGACTCCATCTCTACACC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK093701 unedited GGGTTCACCATTATGTTATACGACTCATATAGGCGGCCGCGAATTCGGCACGAGGGGAAA GGCTGGAGGAGAGAGATGTTTTGCCTGGCAGCTGAAAGCGGACAGAAAGGACAGGAGAAG GTTGGGGAAGTTCTGTTTCCTGAGGCCTGACGCACCCGACCTTCTAACAGAAGACTGTAA CAGGGCTGTGGGGTACACAAGCCAGGTGCCGTGGAGGAAAGCCTGTAGATAGATCCACAT CACGGCACCACCACTTCTCCCCGTGGTTCACAGGCCCTCCCCAAGCATCCCAGGGCCCTC TGCTGTGAAGGCGGCTCCCCCTGCCTCGCTACCTGGGCCCCCGCTCCCCTGTGCAGTGTG CTTATTTCAGTGCTGGTCTGACTTGCTCTTCGTGGTGGTAAGCTCCATGAAGGCAGGACC ATGCTCAGGGAGTTTCCTGTCCTTCACTGCCCCAGGGTTGTGGCAAATACTTAATCAGTG TTTGCCTATGGAGTGAACAGGTCCATGAATGGTCTTGGAAAATGATGTATCCCAGCACCA TGGTGGTGGTCATTCATACGTGTTCTCATGACTTCTTTGCTTTTCCTAATGGTGAGTTCG GTAGGCCAATTCATTTCTTGCTGAAAAGTGTGGTAAAGGCAAGTCTCCTAACTGGACCAA GACTACAGCCTGCAGAACGCGCANGGCCTGTGCATGGGGAAGCACGGATGAGGAGCCTGC TGCTGATGCTGAGCANGAGCCGTGGATGGGTCTACACCACTCTGCTGACTCTGCGTGATG ANGATTCAAAATGCCATCCACGCAAAACAAATTTAGCCACTAAGAAGTAGAGCGGCGCGC TGCTTTAGAAGCCTATGTGTATCACGGTGGTGACACACACCTCTATTTTCTTTTTTGGCT TCAGAAAGAAGGAAAAATATTTTTTTGGGCAATTATTTTTTACACAACCACCCTTCT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK093701 unedited AATAACTATGNACCGCGGCCCGCTTTCTAGNATCGAGTTTTTTTTTTTTTTTTTTGGTGT AGAGATGGAGTCTCACTATGTTGCCCAAGCTGGTTTCGAACTCCTGGCCTCAAATGACTC TCCCACCTCGGCCTTCCAAAACCAAAGTGCTGAGATCACAGGCGTGAGCCACCACACCTG GCCTTAAAACACAAATTTGTTACTTTACAGTTCTACAGGTTGGGAATCCAAAACTAATTT CACTGGGTGAAAATCAAGGAGGTGGCAGAGCTGTGTTCCTTCCCAGAGCTCTAGGGGAGA ATATTTGTTTGCCTTTTCCATCTTCTAGAAGCTGCCCACATTCCTTGGCTTCAGGCCCCT TCATCATCTGGAAAGCCAGCAATGGCAAGTCAAGTCCTTTTCACATGGCATGGCTCTGAT CTCTCTTTGGTGGTCACCTCTGGTTCTGAGCACAGCTGGGAAAGAGTCTCTGTTTTTAGA GGACTCATGTCTCCAGGCCTGCCTGGATGGTCCAGGATAATTTCTGCAACTCAAGGTCCT TAGCCTTAGTCATATCTGTAAAGTCCCTTTTGCTAGTAAGGTAACATATTTACAGGTTCC AGGGATTAAGAAGTGGACACCTTTTGTTGGGGGGCATTATTCTGCCTACCACATCTTTCA TGCAATTAGACAAAACAAACAAAACAAAGCAGTGAATGTTTCAAAATAGGCGCAGAGTTG CTCACATGAATAAATAATGCATAATTTTCACCTGGGCACATGCTTCAACCTGGCCCTGGC TCACAAGTCTGGTCAGGCAGGACACCCAGGATGGTGCTTGTGGAAAGATTTACGCACAGA AGACTCATTCCCCTTCCTCAGCCCAGAAGCAAGTCGGTGTGTACACACCTGCTACACATA GTGCTTCTAAGCAGCGCGCGCTCTACTTA
Restriction Sites	NotI-NotI
ACCN	AK093701
Insert Size	1500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK093701.1
RefSeq Size	1951 bp
RefSeq ORF	1951 bp
Locus ID	55620
Gene Summary	This gene encodes the substrate of breast tumor kinase, an Src-type non-receptor tyrosine kinase. The encoded protein possesses domains and several tyrosine phosphorylation sites characteristic of adaptor proteins that mediate the interactions linking proteins involved in signal transduction pathways. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]

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