ENSA (AF067170) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ARPP-19e
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC102099 sequence for AF067170 edited (data generated by NextGen Sequencing) ATGTCCCAGAAACAAGAAGAAGAGAACCCTGCGGAGGAGACCGGCGAGGAGAAGCAGGAC ACGCAGGAGAAAGAAGGTATTCTGCCTGAGAGAGCTGAAGAGGCAAAGCTAAAGGCCAAA TACCCAAGCCTAGGACAAAAGCCTGGAGGCTCCGACTTCCTCATGAAGAGACTCCAGAAA GGGCAAAAGTACTTTGACTCAGGAGACTACAACATGGCCAAAGCCAAGATGAAGAATAAG CAGCTGCCAAGTGCAGGACCAGACAAGAACCTGGTGACTGGTGATCACATCCCCACCCCA CAGGATCTGCCCCAGAGAAAGTCCTCGCTCGTCACCAGCAAGCTTGCGGGGTAA Clone variation with respect to AF067170.1 279 c=>t 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AF067170 unedited AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCAGCAGCGCAGGTTGTCCC CGTTTCCCCTCCCCCTTCCCTTCTCCGGTTGCCTTCCCGGGCCCCTTACACTCCACAGTC CCGGTCCCGCCATGTCCCAGAAACAAGAAGAAGAGAACCCTGCGCTGGAGACCGGCGAGG AGAAGCAGGACACGCAGGAGAAAGAAGGTATTCTGCCTGAGAGAGCTGAAGAGGCAAAGC TAAAGGCCAAATACCCAAGCCTAGGACAAAAGCCTGGAGGCTCCGACTTCCTCATGAAGA GACTCCAGAAAGGGCAAAAGTACTTTGACTCAGGAGACTACAACATGGCCAAAGCCAAGA TGAAGAATAAGCAGCTGCCAAGTGCAGGACCAGACAAGAACCTGGTGACTGGTGATCACA TCCCCACCCCACAGGATCTGCCCCAGAGAAAGTCCTCGCTCGTCACCAGCAAGCTTGCGG GGTAACCTGAGCCCCCCTCTCCTCCCCTTCCTCAACCACTGGACGTTTATATATTATAGG CAGGGATGAAATGGGCACCTAGTCAGATCTTCTCAGCTTGCTAGCCAGAAATGACTGTGA TTCTGCTGGGGGCTGCTGAGAAGGTAATGTANGTTGAAAAGGGGCTCTAAGTTTATTTAT TTCGTTAGATTGACACTTCCACACACTCCCTGTAGTCCAGGTAGGGCCCANAAATAGGAA AGGCTANGATTGGGATATGCTGCANATGCTTTTTTTGTGTGAGAAACTGGAGAGATGTGA TTTCTCCTTTTGGGAGAGATGTCCCANNATTGATTANGCTGAGCCTTGGGAATAGTTTGG CAGGTTAACATNCCAGGCTACCTAACGTANNTGGAAAGTANNATGATGAGACAATGTTCT TGCTCTTAGAGGTCTGTCCTTAGCTGTATTGCTCAT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AF067170 unedited NGGTACGACTATGNNACCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTTT TTTTTATTTTTTTTTTTTTTTTTGAAAAAAACGTACAAAATTATATATATATTTATATAT ATAATAACATGACATATCTATGTACAACATGGCTGGGACAGTTGAAGAAACTATACAATG GTGTTCAGCATTTTCCCCTTCCCAGATGGACTTTAAGGATGACAGCATGAGGAAATGGAG CAAGAAACACAAAAATTATATACAATTACAAGTGACAGTCAAGGAGTTTGGGGACCAGGG AGTCCAGGGATCCTGCTCTCTCCATTCCTTCCTCACCAACTTTCTCCTATCCAATTTGAA TGACAGCCTGAACACTGAATGGCCAGTCAGGAGAAAGGCATATACACACCTCATCCCCCC ACATGCACATCAGCAAGTCTATCAGTCATTCTCATTGGGCCAAATGTTTGGCATATCAAA ATTTGTGATGTGAGAGGGCAAGAGGATTACAGAATCTTGCTCTCTTGAGCATTAGTTCTC ACCCTGGTCCTCTCTGTCATAACTAGCAAGTTGCGTTCAATAAAATTCTAGCCAAGCTGG TGGTAACTAGATTGAAGATGCTGGTCACCTGCAACCCCTTTCCTCCACCTGTTCATGAGT CAGCATGGCTACCTCTCCTCATGATAAAAGAGCTGGGTTGCTGAATTCTGGGGATGCAGA GATTTTTTAGTCTTTCTTCTCTTCCTCCCTAACCACTTTCCCCAGCTTCCTCCAGGTGAA TAATCTCCTAAGACCTGTTTCTCCACCCTGCAGAATACTTACCCCCAGGGGCTAGCTCAG TTAACCCCTCTCCTCTTTCCTAACAATATACCTGGCATCAGACTGCATTTGGAACAAGGG TCCAAACATGTGCCTGACTG
Restriction Sites	NotI-NotI
ACCN	AF067170
Insert Size	2750 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AF067170.1, AAD32454.1
RefSeq Size	2520 bp
RefSeq ORF	2520 bp
Locus ID	2029
Domains	endosulfine
Protein Families	Druggable Genome
Gene Summary	The protein encoded by this gene belongs to a highly conserved cAMP-regulated phosphoprotein (ARPP) family. This protein was identified as an endogenous ligand for the sulfonylurea receptor, ABCC8/SUR1. ABCC8 is the regulatory subunit of the ATP-sensitive potassium (KATP) channel, which is located on the plasma membrane of pancreatic beta cells and plays a key role in the control of insulin release from pancreatic beta cells. This protein is thought to be an endogenous regulator of KATP channels. In vitro studies have demonstrated that this protein modulates insulin secretion through the interaction with KATP channel, and this gene has been proposed as a candidate gene for type 2 diabetes. At least eight alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

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