Caspase 3 (CASP3) (NM_032991) Human Untagged Clone
CAT#: SC101958
CASP3 (untagged)-Human caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant beta
CNY 3,600.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | CPP32; CPP32B; SCA-1 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC101958 sequence for NM_032991 edited (data generated by NextGen Sequencing)
ATGGAGAACACTGAAAACTCAGTGGATTCAAAATCCATTAAAAATTTGGAACCAAAGATC ATACATGGAAGCGAATCAATGGACTCTGGAATATCCCTGGACAACAGTTATAAAATGGAT TATCCTGAGATGGGTTTATGTATAATAATTAATAATAAGAATTTTCATAAAAGCACTGGA ATGACATCTCGGTCTGGTACAGATGTCGATGCAGCAAACCTCAGGGAAACATTCAGAAAC TTGAAATATGAAGTCAGGAATAAAAATGATCTTACACGTGAAGAAATTGTGGAATTGATG CGTGATGTTTCTAAAGAAGATCACAGCAAAAGGAGCAGTTTTGTTTGTGTGCTTCTGAGC CATGGTGAAGAAGGAATAATTTTTGGAACAAATGGACCTGTTGACCTGAAAAAAATAACA AACTTTTTCAGAGGGGATCGTTGTAGAAGTCTAACTGGAAAACCCAAACTTTTCATTATT CAGGCCTGCCGTGGTACAGAACTGGACTGTGGCATTGAGACAGACAGTGGTGTTGATGAT GACATGGCGTGTCATAAAATACCAGTGGAGGCCGACTTCTTGTATGCATACTCCACAGCA CCTGGTTATTATTCTTGGCGAAATTCAAAGGATGGCTCCTGGTTCATCCAGTCGCTTTGT GCCATGCTGAAACAGTATGCCGACAAGCTTGAATTTATGCACATTCTTACCCGGGTTAAC CGAAAGGTGGCAACAGAATTTGAGTCCTTTTCCTTTGACGCTACTTTTCATGCAAAGAAA CAGATTCCATGTATTGTTTCCATGCTCACAAAAGAACTCTATTTTTATCACTAA Clone variation with respect to NM_032991.2 >OriGene 5' read for NM_032991 unedited
TTCAGCATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGGCGGTT GTGGAAGAGTTTCGTGAGTGCTCGCAGCTCATACCTGTGGCTGTGTATCCGTGGCCACAG CTGGTTGGCGTCGCCTTGAAATCCCAGGCCGTGAGGAGTTAGCGAGCCCTGCTCACACTC GGCGCTCTGGTTTTCGGTGGGTGTGCCCTGCACCTGCCTCTTCCCCCATTCTCATTAATA AAGGTATCCATGGAGAACACTGAAAACTCAGTGGATTCAAAATCCATTAAAAATTTGGAA CCAAAGATCATACATGGAAGCGAATCAATGGACTCTGGAATATCCCTGGACAACAGTTAT AAAATGGATTATCCTGAGATGGGTTTATGTATAATAATTAATAATAAGAATTTTCATAAA AGCACTGGAATGACATCTCGGTCTGGTACAGATGTCGATGCAGCAAACCTCAGGGAAACA TTCAGAAACTTGAAATATGAAGTCAGGAATAAAAATGATCTTACACGTGAAGAAATTGTG GAATTGATGCGTGATGTTTCTAAAGAAGATCACAGCAAAAGGAGCAGTTTTGTTTGTGTG CTTCTGAGCCATGGTGAAGAAGGAATAATTTTTGGAACAAATGGACCTGTTGACCTGAAA AAAATAACAAACTTTTTCAGAGGGGATCGTTGTAGAAGTCTAACTGGAAAACCCAAACTT TTCATTATTCAGGCCTGCCGTGGTACAGAACTGGACTGTGGCATTGAGACAGACAGTGGT GTTGATGATGACATGGCGTGTCATAAATACCAGTGGNAGCCGACTTCTGTATGCATACTC ACAGCACTGGTTATTATCTNGGCGAATTCAAGGAGGCTCCTGGNTCTCCAGTCGCTTGTG CCTGCTGAAACATATGCCGACAGCTTGAATTT >OriGene 3' read for NM_032991 unedited
GGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAGTTTGAAT GTATATTTTGAAATAAAAATGGGAAAATAACAAATTTGTACAAAATTGTCACATTAGAAA CACACTTTAAGATACCATTACATGCTATGTGTATTTACAAAAGTTACAGGTCTAGTAAGA AAAAAGAAACTGTCATTGACAAATGCGAGCTCATTTTTTGAGCAACAAGAAATCTCCCGT GAAATGTCATACTGACAGCCAGTGAGACTTGGTGCAGTGACGGCTCCGCACCTGCTGAGG CCTGGAGCCTGCCTCCCGGGCTGAGTGCTCAGCTCTGGCCTCCGGCTGGATGCCGTCTAA AGTCCTATGTGCTCATAGGTCTTTTTCTTTCAGTAAACAGTATAAAACATTACTATTTTC CTTTGATTGGAAACTTCAACAGAAAATAACATACTTAAGAATTTTGCTACAAAGAGTACT ATGATTTTTATTGCCTCACCACCTTTAGAACATTTCCACTTCACAAGTTTCTGATTTGCT TCACTTTCTTACTTGGCGATGGCGCCCTGGCAGCATCATCCACACATACCAGTGCGTATG GAGAAATGGGCTGTAGGCCCTTCCTGCGTGGTCCATGGCTCTGCCTTCATGGAACAGGCT GAGTCATGTCTCTGCTCAAGCTCAAACCATCTACTCTCAAGTTACAGGCAGCTCAGCCCA TTCATTTATTGCTTTCCTTATATTCCACTGTTTATTATTTAAAGTGTATTTTCATATGTN CCTATAGAAATTATTCACATTACCTGGCTAATAATCACAGTTTGGGGTTTTTATATAATA GCTCTTCAGAATATTGCTGGGTTATGAAGCTCACTGATATGCTAGGGACAATANAANATT TATGCTGTAGATCAAGGCATGTACACCTCAGTAAACTCACCTTATGATGTAGTTACCTTA AGCAGTATCTGCCACTTTT |
Restriction Sites | NotI-NotI |
ACCN | NM_032991 |
Insert Size | 2680 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_032991.2, NP_116786.1 |
RefSeq Size | 2522 bp |
RefSeq ORF | 834 bp |
Locus ID | 836 |
UniProt ID | P42574 |
Domains | CASc, ICE_p10, ICE_p20 |
Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Protease |
Protein Pathways | Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Colorectal cancer, Epithelial cell signaling in Helicobacter pylori infection, Huntington's disease, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, p53 signaling pathway, Parkinson's disease, Pathways in cancer, Viral myocarditis |
Gene Summary | The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. [provided by RefSeq, Aug 2017] Transcript Variant: This variant (2, also known as beta) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 3 all encode the same isoform (a). |
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Other Versions
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RC204444L1 | Lenti ORF clone of Human caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant beta, Myc-DDK-tagged |
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RC204444L2 | Lenti ORF clone of Human caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant beta, mGFP tagged |
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RC204444L3 | Lenti ORF clone of Human caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant beta, Myc-DDK-tagged |
CNY 5,890.00 |
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RC204444L4 | Lenti ORF clone of Human caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant beta, mGFP tagged |
CNY 6,000.00 |
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RG204444 | CASP3 (tGFP-tagged) - Human caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant beta |
CNY 5,200.00 |
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SC322483 | CASP3 (untagged)-Human caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant beta |
CNY 3,600.00 |
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