PDE4 (PDE4B) (AK000989) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DPDE4; PDEIVB
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK000989, the custom clone sequence may differ by one or more nucleotides TAATGCAACTAAAATGTTCTCGGGTTTAGTACCAAGCGTACTAGTATGAGAGGCATGAGATGTTCCGGCA GTATTTACACCTAATTTTATGAGATTTCCAAAACTTGATGATTTCAGGTCCTTCGCAACATGGTACACTG TGCAGACCTGAGCAACCCCACCAAGTCCTTGGAATTGTATCGGCAATGGACAGACCGCATCATGGAGGAA TTTTTCCAGCAGGGAGACAAAGAGCGGGAGAGGGGAATGGAAATTAGCCCAATGTGTGATAAACACACAG CTTCTGTGGAAAAATCCCAGGTATCTAATATGAGATTTTCAAAGTTTTTGTGAGCTCTGCTGATTATAGA GCTGGAGGGTTCTATTTAATTCCGTTTTTCCAATAGAATATGTATATATGAAACAATAAGGAGACAACTA CGCATTTTGTTCCATTATAGTCAGGACTCATGCGACTTCTCCATTACACTCAGTTTCACAGGGTGGGGAA AAGTAGTCATCAGAAACCCATCAAAATTTGGACTATGTGTCCCTAAATCATTTAGATAGTTGTCTCTGAT GTTAGCAAATCCAGCTGAATAATATGAACATCAGTGTCTCCTCCATCCCAACCCTCTGGATTTCTCCTAG CTCTGTCTTCATTCAAGAACTCTACTCCTCATCCTTGCTTCCAGCCCTCTGTTTTTATCTTCTGTCCTTA ATCAAGCCCATGGTGACCATCACCAGAGGCAGTCTCTTTTACTGCATAGTCAAAGCCCTGTCTTGTCTTC CCTCCCAAGGATGCTTCTATGCTTTTGAGAACATGCTGACATTTTCTGGTTGTGTCCAAAGCAACACATT CTGTGTGTGCTCAGGACAGGGGACAATAGTCTGGAGGGGTGAATACAGAAATGTCCAGATGCCAATAGCT GCAGAATATGCAGCAATGGAAAGTATCAGTATCCATACAAAGATTTTTCAAGAAAACGAATGGGCTGAGC CAAGTAGCTGAGATTGAGAATGAGTTCATAGACTGTCCAAGATCTTTATCCCAAAAACATTTGGATTGTT TATTTTCAGTATTTATCCTCTTTCCCTACTACCTGGATACTCCAAATTTGCATATACTTGTAATATTAAT ATACAGTTCCTTTGCAATATTTATTTTGTTGCTTCTTAGAGAAACCCAACTAAATGAGTTTAAAAAATCT GTGAAAGGGCTGGGCACAGTGGCTCATGCCTGTAATCCAGCACTTTGGGAGGCTGAGGTGGACGGATCAC CTGAGGTCAGGAGTTCAAAACCAGCCTGGCCAACATGGTGAAACCCTGTCTCTACCAAAAACACACAAAA AATTGGTCGAGCATGGTAGTGTGTGCCTGTAGTCCCAGCTACTCGGGAAGCTGAGACAGGAAAATTGCTT GAACCCAGGAGGCGGACGTTGCAGTGAGCTGAGATTGTGCCACTGCATTCCAGCCTGGGCAACAGAGCAA GACTCTATCTC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK000989 unedited NGGTTCACATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGCAGGAAGCAGC GTCAGACACTCAGGAAGAGGTTATTGACATGGTGTTAGCAACTGATATGTCTAAACATAT GAGCCTGCTGGCAGACCTGAAGACAATGGTAGAAACGAAGAAAGTTACAAGTTCAGGCGT TCTTCTCCTAGACAACTATACCGATCGCATTCAGGTCCTTCGCAACATGGTACACTGTGC AGACCTGAGCAACCCCACCAAGTCCTTGGAATTGTATCGGCAATGGACAGACCGCATCAT GGAGGAATTTTTCCAGCAGGGAGACAAAGAGCGGGAGAGGGGAATGGAAATTAGCCCAAT GTGTGATAAACACACAGCTTCTGTGGAAAAATCCCAGGTTGGTTTCATCGACTACATTGT CCATCCATTGTGGGAGACATGGGCAGATTTGGTACAGCCTGATGCTCAGGACATTCTCGA TACCTTAGAAGATAACAGGAACTGGTATCAGAGCATGATACCTCAAAGTCCCTCACCACC ACTGGACGAGCAGAACAGGGACTGCCAGGGTCTGATGGAGAAGTTTCAGTTTGAACTGAC TCTCGATGAGGAAGATTCTGAAGGACCTGAGAAGGANGGAGAGGGACACAGCTATTTCAG CAGCACAAAGACGCTNTGTGTGATTGATCCAGAAAACAGAGATTCCCTGGGAGAGACTGA CATAGACATTGCAACAGAAGACAAGTNCCNCCGTGGATACATAATCCCNCTCTCCCTGTG GAGATGAACATTCTATCCTTGATGAGCATGCCAGCTATGTGGTAGGGCCAGCCCACCATG GNGGCCAAGACCTGCACAGGACAAGGGCCACCCTGCCTTTCAGTTACTTGAGTTTGGAGT CAGAAAGC
Restriction Sites	NotI-NotI
ACCN	AK000989
Insert Size	2900 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK000989.1
RefSeq Size	1481 bp
RefSeq ORF	1481 bp
Locus ID	5142
Protein Families	Druggable Genome
Protein Pathways	Progesterone-mediated oocyte maturation, Purine metabolism
Gene Summary	This gene is a member of the type IV, cyclic AMP (cAMP)-specific, cyclic nucleotide phosphodiesterase (PDE) family. The encoded protein regulates the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. Altered activity of this protein has been associated with schizophrenia and bipolar affective disorder. Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]

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