SRSF1 (AK054610) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ASF; SF2; SF2p33; SFRS1; SRp30a
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK054610, the custom clone sequence may differ by one or more nucleotides CTATTAAATTTGTTAATTGTCAGTTCTGGCTTTTTTGCATAAAGAGTTGGTCCATTAACTTGCCAATTTG AAGCTTCTAACTAGATATTCCCTACTGAAAGTTTTGGATTTGTTTTTAGTTTGTGGAGCAGTCTTAGCTG GGGACAGGTAATTGACAACGGCAGAGATACTTTCTTTTCCTAGGATTCTAAGTCTGTAATCCACATCCTC AATGTATTCACAGGACTTTAAAATTCTCTCCAAATGAGGAAGGAAATATCCTGTTGCTTTCTAATGTTTA CTAAAAGTTGTGTTTAGAACAACAGATTTTAATAGGCATCTTCCTTTGTTATGTGTCATTAGCCCTTTGC CCGTTTACCTTAGGGCTCTTTGAAGGAGAAATGGATGGGAGAAAACCTGTCACTTGGCGAAAGTAAAAGG GATAATTAACTGGCTCAGAGCTTATGTGCAGAGTTCCAAGCCCCAAAGTTAATCTAGAACCACTCGATAA CACCAATAAAAATATTTATTTCACATCTGTTATATATCTGGAAAATGTTCTAAGCATCTTACACATATTT CTCATTAAATCCACAGGTGACCATTGTGAGGTAGATATTTTGTTCTAATTTTCCAGATGAGGAAGCTGAG ACCCTAAAAGGTTAGGTGACAGGTTATACAACTTGGAGTGTGGGAGGAGGAGAGAGGAACCTGAACAGGG CAAGTTGGGGATCTGACTTTTGTTTGGGTAGATGTAGGCACATTGTATTTTTGGCTTAGATGCTTTATTC ATCATGGCTGAAGGTAATACCATTTACTCACTCACCGAAAATTGTTTACAATAATCTAGATGAATTTGCT GTCTTTGGACATCTGTCTTTTGACTGGACCCCAGTATATAGTCTGTGGAAGCTCACTTAAGGAGAGAGCT CCTTTTTGTTTGGTTAGAGAAATTTTCTGTCCTAAAAGTAGAAATAGCCCCTTCTAGGTAAGGATGGAGC ATTTGATCATACTGGTTTCATTATATTCCTCTAACAGTTGGAACCGATTGTTTTTGAGTACTTGTTTCAA ACTTCTGAGTATTTTCCTTCTGGAAAATAGCTCAGTGTTTAAAATTTACATGAACTTAAAAGGTTAATTT TTTTTTAAAGAATGTTTGAGGAGCAATCAATTCTGATTTTGTCTTATGGATAGAAATAGAATTAGCCATT TTCCTCAAGAAAAGTGATTCTTCTGAATCTTAGCTTTTCAGTAATGAGAACTGGACTGTTTCCACTTGAC CTCTATTTCCTGTTAGTGGGAAACCGCTTCCCAGAGGTGCTGTGTGAAAGATTGCTGGGGAAAGGGTTTC TAGATCCCAGCATAACAAGTTCAAGAGTCCAGCGATATTGTCATTAGAATAATGGTCTTCATTAAGTACT AGTTTTCATAATCCTGAAGCTTCATTTTCACCAATTTGAAAGGCAGCTAGGGAGGAGAAGACTATGCAGG GAAGGAGGAAAACTCTGGAGTGCATCACAGAGACCTTGAGTGAGTTAAGGCAGGGAAGAAGCAGATTTGC TATCCACTGAATGAAAATAAGAAGAATTCCATTGTCCAAGGTCAGAGCAAGAACACAAATGTTTTAGTTT ACTTAGTTAATTCCTTAGGTTTGGACATTACAGGAGACATGGGGGTGACCATAGCAATTTAGCTGATAAA TGTATCAGAAACTCTTTCATCATTTTCTGTCCCCTCCTATCTTAGGCTGACCGGTTCCCTGATGTGTTAC CTGCTTCTGCTACTGATCCAAACTGCAGAACTTCTCATTCATCCCCAAGGCCTCCAGGCAGTATCCAATG GGGAATCAGCTCTAAAAGGAACCAGACCAACGTTTTCCAGCCCCTTCATTCTGGTGACTGAGGGGAGGAA AGAATGGGAGGGGGTATTCTTGTCTAGTGGATGGAAAGGAAACACACTGTCAAATTACTATATCTCCTTG GTTTTCTATTACAGTAGAATTCTCCAGCCATATTTTTATTGTCTATGGGGGAAGTTGGAGATGGTGACCT TGATTAGAAGTGTCTGGAGGGGGATAAATGGAGGGGATAAGATTCAGTTGGTTTTGGAAAATGTTAAAGT CTTAAAATAATGCGTCCATCTGAAGGATTTTTTCTAAAACCAGAGTTTATAAAAATATCACTGATACAGC CTGCCCCCTCATTTCCCTGCCACAGGAGATGTCTTGGACTAGAGACACTTGTTTAATAATAGCTTGTCTC TGATATTCCCAGTAGCTTCCCTCTGTGTGAGGAAAGGATAGAAATGTTCAGGACATCATCATACAGGCTC CTCATCTACAAAGTTCCAGTAGCAGTGACGCCTACACGGAAGACTTGGAACTGCAAACAGGCTGGGGTCA CCTCAGTGACATCTGACGCTGTCCAACCAGAAGTTCGATTTTTGTTCTGGGGGTGAAGGAGGAAACAGAC TGTACTAAAGGACTAAAATAATTTGTCTATACT 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK054610 unedited TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTTGCTTTTTCTCCTG ATTGTATTGAGGCTGATATGGAAGGGTTATTTCTTTCTGGCCAATACTTTTTGGTATTTC TAAATATTGCAATCTTGATTTTTACTATTAAATTTGTTAATTGTCAGTTCTGGCTTTTTT GCATAAAGAGTTGGTCCATTAACTTGCCAATTTGAAGCTTCTAACTAGATATTCCCTACT GAAAGTTTTGGATTTGTTTTTAGTTTGTGGAGCAGTCTTAGCTGGGGACAGGTAATTGAC AACGGCAGAGATACTTTCTTTTCCTAGGATTCTAAGTCTGTAATCCACATCCTCAATGTA TTCACAGGACTTTAAAATTCTCTCCAAATGAGGAAGGAAATATCCTGTTGCTTTCTAATG TTTACTAAAAGTTGTGTTTAGAACAACAGATTTTAATAGGCATCTTCCTTTGTTATGTGT CATTAGCCCTTTGCCCGTTTACCTTAGGGCTCTTTGAAGGAGAAATGGATGGGAGAAAAC CTGTCACTTGGCGAAAGTAAAAGGGATAATTAACTGGCTCAGAGCTTATGTGCAGAGTTC CAAGCCCCAAAGTTAATCTAGAACCACTCGATAACACCAATAAAAATATTTATTTCACAT CTGTTATATATCTGGAAAATGTTCTAAGCATCTTACACATATTTCTCATTAAATCCACAG GTGACCATTGTGAGGTAGATATTNTGTTCTAATTNTCCAGATGAAGAAGCTGAGACCCTA ANAGGTTANGTGACAGGTTATACACTTNGNAGTGTGGGAAGGAGAAAGAAGAACCTGACA GNGGCAGTNGGGATCTGACACTTGNTTGGGTAGAGTAGCACATTGTATTTTGGNCTT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK054610 unedited AATAGCTGTGNACCGCGGCCCGCATTTCTANGATCGAGTTTTTTTTTTTTTTTTTTGACA AGAAATATTTTAGTCCTTTAGTACAGTCTGTTTCCTCCTTCACCCCCANAACAAAAATCG AACTTCTGGTTGGACAGCGTCAGATGTCACTGAGGTGACCCCAGCCTGTTTGCAGTTCCA AGTCTTCCGTGTAGGCGTCACTGCTACTGGAACTTTGTAGATGAGGAGCCTGTATGATGA TGTCCTGAACATTTCTATCCTTTCCTCACACAGAGGGAAGCTACTGGGAATATCAGAGAC AAGCTATTATTAAACAAGTGTCTCTAGTCCAAGACATCTCCTGTGGCAGGGAAATGAGGG GGCAGGCTGTATCAGTGATATTTTTATAAACTCTGGTTTTAGAAAAAATTCTTCAGATGG ACGCATTATTTTAAGACTTTAACATTTTCCAAAACCAACTGAATCTTATCCCCTCCATTT ATCCCCCTCCAGACACTTCTAATCAAGGTCACCATCTCCAACTTCCCCCATAGACAATAA AAATATGGCTGGAGAATTCTACTGTAATAGAAAACCAAGGAGATATAGTAATTTGACAGT GTGTTTCCTTTCCATCCACTAGACAAGAATACCCCCTCCCATTCTTTCCTCCCCTCAGTC ACCAGAATGAAGGGGCTGGAAAACGTTGGTCTGGTTCCTTTTAGAGCTGATTCCCCATTG GATACTGCCTGGAGGCCTTGGGGATGAATGAGAAGTTCTGCAGTTTGGATCAGTAGCAGA AGCAGGTAACACATCAGGGAACCGGTCAGCCTAAGATAGGAGGGGACCAGAAATGATGAA AGAGTTTCTGATACATTTATCAGCTAAATTGCTATGGTCACCCCCATGTCTCCCTGTATG TCCCAACCTNAGGAATTACTAAGTAACTAAACCTT
Restriction Sites	NotI-NotI
ACCN	AK054610
Insert Size	3000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK054610.1
RefSeq Size	2483 bp
RefSeq ORF	2483 bp
Locus ID	6426
Protein Families	Stem cell - Pluripotency
Protein Pathways	Spliceosome
Gene Summary	This gene encodes a member of the arginine/serine-rich splicing factor protein family. The encoded protein can either activate or repress splicing, depending on its phosphorylation state and its interaction partners. Multiple transcript variants have been found for this gene. There is a pseudogene of this gene on chromosome 13. [provided by RefSeq, Jun 2014]

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