H6PD (AK026460) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CORTRD1; G6PDH; GDH
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK026460, the custom clone sequence may differ by one or more nucleotides ATTCCAGGCTCCTCCCTGTGTCTTTGCTGTTCTTCAGACTCCATTTATAGAGAATGAGGGCTGATAACAG GAATACAGTGGCAAAGACTAGACTGTGGAAAGGGTTCCAGAAATCTTTTTTTCTTTTTTAATTAAAAAAA ATATTTGCAGAGATGAGCTCTTGCTATGTTGCCCAGGCTGGTCTCAAACTCCTGGGCTCAAGCGATCCTC CCATCTCAGCCTCCCAGAGTGCTGGGATTACAGGTGTGAGCTACTGCGCCCAGCCCCAGAAATCTCAGTG CTGTTTGGAGCTCCATTTCTCATTTGATGACTTGCTCTGCGTGGGGAGGTGGGGTCTCATTCCCCCAACT TCCTCAGGGAGGACCCCTGCCCTCCGCTGCTCCTCTGTCCTGCTAGCCTTCCTCCAGGAAGCACACTGGG TGCAGATAATCAGGACATTCCAGAGATCCCCAATTTAAGAGGGTCATTTCCATCTCAGGGGACTCCCGGA TGGGTGTTTCCGCTCTCAATAGCCCCTCTTGTTTTACCAGGAAAGATCCAGTTAAATCACCCACTGAGGT GACAGCTCATTAGCGGGGAGAGAGATGGAGCTTCGAGTGACACTGGGCCATCCAGGCGGCTCTGCTCCCA CCAGACAGGAGCTAGGCCTCACTGGCAGGGGGGCTGCCCACAGCCTTTTCAGGGGCTCGCTTGGCGGGTG ACGGGGCCGCAGCCAGGCCTTCTCTCCCTGCCCCTTGGTGACCCCGTGGCTTCCTGTCTGCTGGCCTCTC CTGCTACTTATCACTTCACCACGAACTCTCTGCCTGAGACTGGGGAAGTAAGCGGGTATCTTCTCAGTGA GCATAGGTTGGGGACTGTGATCTTGAGAAGCCATGGGCCAGCAATACCTGCTTTTCTGAAGCCCCCAAGG AGGGCTCTGACATTCTTTTTAAAAACACCACAAAGCAAAATTCCCAGGACATGTGTAGTTTTGTTTGTTC AGTATCCCACAACTTAGGGCTGGGAGATGGAACTCTTGGTTAAGGTCGATTTTTCTGTCTGGCTTCTCCG CACCTTCCACTTGCTCTCTGGATCAGGCAGATATAAACTTTCTAGCGCATTTTGAGAGAGGGCTTTCTTG GGTGAGGGAGCATGGCAAAGTCGGTTTCTCTCTGGACTGTTTACACTTCAAGGCGGTGGATTTAGAGGAA TCCTGGCTTTCATTTTCAATGCCAGTCTGAGACATGTTCCCAAGCCGGGGCTCTTGTTCACACCACTTAC TCTGGCCACCAACAACAACCCAGGCCAGACAGAGCATCTCTTTTTTTTTTTTGAGACAGAGTCTCTGTCG CCCAGGCTGGAGCCAGTGGCGAGATCTTGGCTCACTACAACCTCCACCTCCCGGGTTCAGGCAATTCTCG TGCCTGAGCCTCCCGAGTAGCTGCGACTACAGGCGCCGGCCAGCATGCCTGTCTAATTTTTGTATTTTAG TAGAGACAGGGTTTCACCATGTTGCCCAGGCTGGTCTCGAACTCCTGAGCTCAGGCAGTCTACCCACCTC AGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCGCGCCCAGTCAGAACATCTGTTTTTACACCCA GAGAGCGCCCCTCGTTAGGACAGAACCACGGTGCCCAGAGCCAGGAAGCCGCCCTCCTGGCGCCCAGCAT CTGAGCTTCTACACGTGATGGGCGGGCTCAGGAGAGGACAGGGAGTCGTGGTGGAAGTTCCACAGCTGGC CGCTTGGGGGGGCCCTTGCACCGCACTGCCGCCTCCTGACTGCCCCGATCCCCGCAGCCCCTGTGCCGGA TTTCATTTCCCTCCTCTCTCCCAGGGTACCTGGCCCCAGCACTCTCCCATCTGTTCTTCAGGAACCGACT CCTCTCCAGTTGCAACACCAGGGAGAAAGGGGCCTCCACATGCCCAAGTACCCCTGCAGGATGAAGGGCA GGCCGGCCCTTGATGTGCCATTTCTGAATAATAGTCACTGCCGCCGAGTCTAGGATGTCCTGTTCTAACT CAGCCCTGCCTCGGATGCACCACCGATCTGTGCAGAGTGGGTGTGGGAGTGTGGGTGAGGGTCGAAATGC CAAAGGTCTACTTTCCAGAATCAAGTGCCTTCTGCAAATCATGTTGGAAAAGTCCAAACCTGGAGATGTC CCTGTGCCTCCGCCCCTACCCACCCCTTTTCCTTCAGCTGTGTTAGGAAGGAGAAGTTTTCAGAACCCTA TAGGCTGGTGGCTTTCAAACTTCAGACCATGATCTGCAGCAAGAAACGTGCCTTCCATCATAAATCAGTC CATTTGTTTACAACTGTGTTCCAAGCAGGTTTCATAAAGAAATTCTTACCCTTAGAACCTCGGATATCCT CTATGTTTTAGTTTTCATTTTTTTAAAATGCTTCTTAAAATTCACTAAATTGGGCTAGGTGTGGCTCATG CCTGTAGTCCCAGCACTATGGGAGGCTGGGGTGAGAGGATCACTTGAGCCCGGGAGGCTGGTCTCGAACT CCTGACCTCGGGTGGTCCATCCGCCTCGGCCTCCCAAAGTGCTGGGATTGCAGGCGGGATCCACCATGCC TGGCCAGAACAAATGCCTTTTTAAACCTTTTAAGAACATTTTTTAAATGTCTTTTTCTATGTCAAATGTA ACGTTTATTTTTTTAAACAATAAAATTGATTTGCCAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK026460 unedited GGTGTTCACATTTGTATACGACTCATATAGGCGGCCGCGAATTCGGCACGAGGCTACTTA TCACTTCACCACGAACTCTCTGCCTGAGACTGGGGAAGTAAGCGGGTATCTTCTCAGTGA GCATAGGTTGGGGACTGTGATCTTGAGAAGCCATGGGCCAGCAATACCTGCTTTTCTGAA GCCCCCAAGGAGGGCTCTGACATTCTTTTTAAAAACACCACAAAGCAAAATTCCCAGGAC ATGTGTAGTTTTGTTTGTTCAGTATCCCACAACTTAAGGCTGGGAGATGGAACTCTTGGT TAAGGTCGATTTTTCTGTCTGGCTTCTCCGCACCTTCCACTTGCTCTCTGGATCAGGCAG ATATAAACTTTCTAGCGCATTTTGAGAGAGGGCTTTCTTGGGTGAGGGAGCATGGCAAAG TCGGTTTCTCTCTGGACTGTTTACACTTCAAGGCGGTGGATTTAGAGGAATCCTGGCTTT CATTTTCAATGCCAGTCTGAGACATGTTCCCAAGCCGGGGCTCTTGTTCACACCACTTAC TCTGGCCACCAACAACAACCCAGGCCAGACAGAGCATCTCTTTTTTTTTTTGAGACAGAG TCTCTGTCGCCCAGGCTGGAGCCCAGTGGCGAGATCTTGGCTCACTACAACCTCCACCTN CCGGGTTCANGCAATTCTCGTGCCTAAGCCTNCCGAGTAGCTGCGACTACAGGCGCCGGC CAGCATGCCTGTCTAAATTTTGTATTTTAGTAGAGACAGGGTTTCACCATGTTGCCCAGG CTGGTCTCGAACTCCTGAGCTCAGGCAGTCCACCCACCTCAGNCTCCCAAAGTGCNTGGA TTACAGGCGTGAGCCACCGCGCCCAGCCAGAACATCTGTTTTACACCCAGAGAGCCCCCT CGTTAGGACAGACCCACGTGCCAGAGCCAG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK026460 unedited TACATTATGNACCGCGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTCTAAGTTA AGAATTTCTTTATGAAACCTGCTTGGAACACAGTTGTAAACAAATGGACTGATTTATGAT GGAAGGCACGTTTCTTGCTGCAGATCATGGTCTGAAGTTTGAAAGCCACCAGCCTAGAGG GTTCTGAAAACTTCTCCTTCCTAACACAGCTGAAGGAAAAGGGGTGGGTAGGGGCGGAGG CACAGGGACATCTCCAGGTTTGGACTTTTCCAACATGATTTGCAGAAGGCACTTGATTCT GGAAAGTAGACCTTTGGCATTTCGACCCTCACCCACACTCCCACACCCACTCTGCACAGA TCGGTGGTGCATCCGAGGCAGGGCTGAGTTAGAACAGGACATCCTAGACTCGGCGGCAGT GACTATTATTCAGAAATGGCACATCAAGGGCCGGCCTGCCCTTCATCCTGCAGGGGTACT TGGGCATGTGGAGGCCCCTTTCTCCCTGGTGTTGCAACTGGAGAGGAGTCGGTTCCTGAA GAACAGATGGGAGAGTGCTGGGGCCAGGTACCCTGGGAGAGAGGAGGGAAATGAAATCCG GCACAGGGGCTGCGGNGATCGGNGCAGTCAGGAGGCGGCAGTGCGGTGCAAGGGCCCCCC CACGCGCCAGCTGTGGGACTTCACCACGACTCCCTGGTCTCTCCTGAGCCCGCCCATCAC GTGTAGAAGCTCAGATGCTGGGCGCCAGGAGGGCGGCTTTCCTGGCTCTGGGCACCCGTG GTTCTGTTCCTACGAGGGGCGCTCTCTGGGTTGTAAAAACAGATGTTCTGGGCTGGGCGC CGGTGGCTCACCCCTGTAATCCCACACTTTTGGAAGGCTGAGGTGGGTGGACTTGCCTTG ACTCAAGAATTTCGAGACCAGCCGGGCAACATGG
Restriction Sites	NotI-NotI
ACCN	AK026460
Insert Size	1850 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK026460.1
RefSeq Size	2712 bp
RefSeq ORF	2712 bp
Locus ID	9563
Protein Pathways	Metabolic pathways, Pentose phosphate pathway
Gene Summary	There are 2 forms of glucose-6-phosphate dehydrogenase. G form is X-linked and H form, encoded by this gene, is autosomally linked. This H form shows activity with other hexose-6-phosphates, especially galactose-6-phosphate, whereas the G form is specific for glucose-6-phosphate. Both forms are present in most tissues, but H form is not found in red cells. [provided by RefSeq, Jul 2008]

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