NUAK2 (AK091893) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK091893, the custom clone sequence may differ by one or more nucleotides AAGTGACACCTCCTCTAGGAAGCCTTCTTTGATCACCCCAACCTCTCCCAGACCTCTAATGTGGACCCCA CAGTCCCTGGACTTCTTTGAGACCACACTAGCCCACTCCCCGCCCCCTCCTGGAACCTTGAGCTTCTGGA GAGCTGGGGCCACACCGTCCTCCTGTGGTTTTCCGAGCCGGTGCAGGTGGTCAGCAGATAACCTTGAGTG AATGTGAATCTAAAAAAAGTAATAAAGGGATCTGGCACACTTAACTTTTAAATCTAAATATGTTTCTTTT AAATTCTATGATTGTTCTGGGTCTTGAGAGCAGACCTCCCCAACAATTCGGTCTCCCATATGGGGTCACA GCCGTTTGGGTTTCCGATCGCGGGCACGGCACCCATCTGCGGTGTCTGTGAAGCCGGCGCGTTCTGCCGG GGGTTTGGGTTTTTTTGTGTGTGTGATAGATCTTTTTAGCTTAGATACGTTTCTTAAAGCTTCTCTTTGT TTAAAAATGCGCAGGAAAACTTCGGCGTCGTTCTATTTTTGTCCTTTCTAACACCTACCCCTGATTTTTC CTTCTTTTTTTATTTCCTTGCAGACTTTGAATTTTAAACAGATAAAAATGGATGCTGTTTCCATCGTTTG GTGTCCTGCTTTTCTACGTAGAGGAAACATTTCTCAGCACAATTAAAACGTGTTCGTATGCTTATTAATG TCTTTGTAGGATTTCATCCTGTGAATCATTTGCTTGTCCAGCAATAAGGAAATGGCTTTTTGCGTCTGCG TTTTGATGATGGAATTCCCCATGGGCTTCAGAGTGCGGTGCCTGCCTTTCAGATGATTTCCTTAGGACCG AGTTCCTGGGACTTGCTGCTTCATGGCCAGCGTCTCGGGCCCCTGTCAGCTCTGGCCTGTCCTCCCTGGG GTGATGGCCCTGCCGGGAGCCGTCTGTCCTTGGCAGATGGAGTTCACTGTTAGTGAGAGCCCAGCCTCTG TTTTACAAACGAGCATGCTCCAAGCAGTGGCTGATGGGCGGTTGGAATTCACCATGGCTCTGGCCTCTGG TTCCTGTAAATTGAGGCGTAGGAAAATTAGTCCTCCCCGGGTCCTCTGGTTTACAGAATGCGGGGATGTC CAGGTTTCTCCTGTCCTCCTCATCCCTGCACTGGTTCCACGGGACTGGCTGGACACAGCGTCACTGGTTC CCAGCACAGCTGACACCCCTTCCAGGAACCTTCTCACTATCCCCAGGGCAGAGGTGGCCTTCCGGGCATG CGTGCTCCTCTCCCGTCTCCTCTGCAGCTTCCTTTTAGTCCAGTGGTGTTTCAGGTGTCTTGCTCTTACT GGAGCTCCTTAAGGACAGGGCCTGGATCTCAGTCATCCCCAATCCCCAGCTAACCCCAGCTCATGTTCCT GATTGGCCCTGGGACACTAGCAGAACGTGACTTGGTTCTGTTCACTAGTGCCATATAGAGTCAGGTTCCT GGAGCTGGTGTGAGAGTGGGAGGTTTGAATCTTCATGGGCAGGGGCTACATTGTGTCAGGCTCTGAGCTT GGCATTTTCCATGTGAAAGGAGACAAGGGCTCCAGGAGAAATGGCCTGTATTTGCTGGGGTGTAGGGGCG GGCCACATGCCACTGAGCTTTAGAAGCCTGCTGGGTTAGGAATCAGCACACAGCCTGGATCAGTGAAAAT ACAGGAACAAGAAGATAATAGGAGAGTTTGTGCTGTAAGGCAGGTGTTTACAGTTTGATTTTTCTTCAGT GAGTAGAAATGTTGGCGTAGCTTGAAAAAAAATCTGAAATAATTCACTGTCCACGGAAAGGGCTGGAAAG GAGTTATCTTAAATTCATATAGATTAAAGAAAGCATAGCTGGTCGTGGTGGCTCGCGCCTGTAATCCCAG CACTTTGGGAGGCTGAGGTGGGCGGATCGCCTGGGGTTTGGAGTTCGAGACCGGCCTGACCAACATGGAG AAACCCCGTCTCTGCTGGGAATGCAAAATTAGCCGAGCGTGGTGGCAACGCGCCTGTAGTCCCAGCTACT CAGGAGGCTGAGGCAGGAGAGTCGCTTGAGCCCGGGAGGCGGAGGTTGCGGTGAGCCGAGATCGCGCCAT TGCGCTCCAGCCTGGGCAACTAGAGCAAAACTGCGTCTC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK091893 unedited NGGGGTTCAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCTGTGA TGTCTTTTCCTTCCTCCCTCTGCCCCTCTCCCTTCTTCTTGAGAATTAAATGCCTGAAAC CGATTAGTTGAATACCCCTCTCATTCTCTTGCCCAAGTGATAGTTCCCACCACCTGGAGG ATGAAGTCCAGACCAGTTGGTGTGGCCTACGGGGCGTGCAGGATCTGGCTGCTGCCTGCA TCTCTGGCCACCAGTGGTGCCAGTGCTCAGAGCCAGTGACGCCCTGCCTCTGCACCTTGC TTGTTCTGCTGCCTCTCCCGGGCAAATTCTTGTTTGTCCCTTAGGATTCAGCTTAAGTGA CACCTCCTCTAGGAAGCCTTCTTTGATCACCCCAACCTCTCCCAGACCTCTAATGTGGAC CCCACAGTCCCTGGACTTCTTTGAGACCACACTAGCCCACTCCCCGCCCCCTCCTGGAAC CTTGAGCTTCTGGAGAGCTGGGGCCACACCGTCCTCCTGTGGTTTTCCGAGCCGGTGCAG GTGGTCAGCAGATAACCTTGAGTGAATGTGAATCTAAAAAAAGTAATAAAGGGATCTGGC ACACTTAACTTTTAAATCTAAATATGTTTCTTTTAAATTCTATGATTGTTCTGGGTCTTG AGAGCAGACCTCCCCAACAATTCGGTCTCCCATATGGGGTCACAGCCGTTTGGGTTTCCG ATCGCGGGCACGGCACCCATCTGCGGTGTCTGTGAAGCCGGCGCGTTCTGCCGGGGGTTT GGGTTTTTTTGTGTGTGTGATAGATCTTTNTAGCTTAGATACGTTTCTTAAAGCTTCTCT TTGTTTAAAAATGCGCANGNAAACTTCGGCGTCGNTCTATTTTTGTNCCTTNTTACACCT ACCCCTGATTTTNCTTNCTTTTT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK091893 unedited NGGGTAATCTATGTACCGCGCCGCAATCTAAGATCGATTTTTTTTTTTTTTTTTTGAGAC GCAGTTTTGCTCTAGTTGCCCAGGCTGGAGCGCAATGGCGCGATCTCGGCTCACCGCAAC CTCCGCCTCCCGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTACA GGCGCGTTGCCACCACGCTCGGCTAATTTTGCATTTTTAGTATATACGGGGTTTCTCCAT GTTGGTCAGGCTGGTCTCGAACTCCAAACCTCAGGTGATCCGCCCACCTCAGCCTCCCAA AGTGCTGGGATTACAGGCGTGAGCCACCACGACCAGCTATGCTTTCTTTAATCTATGTGA ATTTAAGATAACTCCTTTCCAGCCCTTTCCGTGGACAGTGAATTATTTCAGATTTTTTTT CAAGCTACGCCAACATTTCTACTCACTCAAGAAAAATCAAACTGTAAACACCTGCCTTAC AGCACAAACTCTCCTATTATCTTCTTGTTCCTGTATTTTCACTGATCCAGGCTGTGTGCT GATTCCTAACCCAGCAGGCTTCTAAAGCTCAGTGGCATGTGGCCCGCCCCTACACCCCAG CAAATACAGGCCATTTCTCCTGGAGCCCTTGTCTCCTTTCACATGGAAAATGCCAAGCTC AGAGCCTGACACAATGTAGCCCCTGCCCATGAAGATTCAAACCTCCCACTCTCACACCAG CTCCAGGAACCTGACTCTATATGGAGCTAGTGAACAGAACCAAGTCACGTTCTGCTAGTG TCCCAGGGCCAATCAGGAACATGAACTGGGGTTAGCTGGGGATTGGGGATGACTGAGATC CAAGCCCTGTCCTTAAGGAGCTCCAGTAAGAGCAAGACACCCTGAACACACTGGACTAAA AGGAAC
Restriction Sites	NotI-NotI
ACCN	AK091893
Insert Size	2500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK091893.1
RefSeq Size	2139 bp
RefSeq ORF	2139 bp
Locus ID	81788
Protein Families	Druggable Genome, Protein Kinase
Gene Summary	Stress-activated kinase involved in tolerance to glucose starvation. Induces cell-cell detachment by increasing F-actin conversion to G-actin. Expression is induced by CD95 or TNF-alpha, via NF-kappa-B. Protects cells from CD95-mediated apoptosis and is required for the increased motility and invasiveness of CD95-activated tumor cells. Able to phosphorylate 'Ser-464' of LATS1.[UniProtKB/Swiss-Prot Function]

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