CEP162 (AK093579) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK093579, the custom clone sequence may differ by one or more nucleotides ATCCAGAAGCCATTTGAGATGAAGAGAACATTTACTAATACAAATGAAAACCAATCACTTAGGCGGTTTC CCCCTTTGATGCATAAGAAATCATGATGCGGTCTTTGCTTGTCTCTGTTCTCATCCGGCATTTTTATATT TGCTAGGCGCAAATAAAGTACGATTCCAATGAATAAATAGAAGTGTTAACACTTCCTTACACTTTTACCT AAACCAGCTTGGATGAGGAAAGGAAGTGTCTTTAGAAATCTGTTCTGAGAAGTACTTTCACCTGAGGAAG CTGATAATTGAGATGAGAGTTGTTTTCATAAGCAGTTGAGCGTGCATGGTGGTGAGGTATGAGGAGTGGC GGTGAGAGTGTGGGTATCGTGCGCGTCAGTGTGAGCAGCAGTGCCCGTTGGTGGGGTGTGAGTGAGCGTG AGGATGGTGACACGCTCTTGCCTGGCATTGTGCCTCTGTGTCTGGTCAAGGCCACATTTGCCAAGGGAAC TGGAGCAGACTTTTTCTACATTCTTGCCGGCAGCCCTGGGGGTTTTAAGGCCCACAGGAAAGTACTGTAT GAGAAATTAGGATCTGTGCTTTTCATTTTTTGTTCTGAGTCTTTGTTTTTATGATATCTTTCAGGTAACC ATGTGTGCAAGTCAGAATTTCCATTTTCTATCATGGGAAGTTACTTGAAGAATGTTGCTGTTCCTTATTG TATACTTTAGTGTGCACAGTCTAAAGAAAAAGTGAGCTACTACAAGAACTGGTGGAGACTGAATTACTAG GGAAGAAAATTGGGATAAAACTGTTGGGCAAAGAAGTAGAAAGGGCAACGGAAGGGGTATTGAAGTGAAG ATAAACAATTTTTTTTGATAAGTCTGGCTGCATTTGCTTTGCCAACTCCACACTGGGAGAGAAGGGCTCA CACCGAGCAGCTGCCGTCCAGTGTTGCTGAGCACCGCCCAGCAGTAGCGTGGAGAGTGATCTTGCAGGGT GTGAGAAGAGGACATGGAGGCTCGTGGAGGCCGCTCTGCAGCCCGCTCTGCCTGGCTCTGGCTCAGGTGT GCTGTGTGCTCTGTGACCCTCTGCGCCTGCAGGTGCCCTCGGAGCACGTGAGGGGCGGGCGTTGGCTCTT CAAGGCTCTCCATCTCAATTTGCTTCGAATTAGGGAAGCCATGAGCACATGTGTATAAATGTGTCTTCTG GAACAGATTGCTGCTGCATTTCTAAGAGCTTTCCAATTTTTTATAGAAATACTTTTGTTTAACTTTTTGA AGGCTATTTTAGATTCAGAGTTTGTTACGTGGCTGTATTGGGTGATGCTGGGGTTTGGGCTTCTGGGGTA CCTATTACCCAAATAGGAAACATTGTACCCAATGGGTAGCTTTTCAAAACTCACCCCCATCACCTTCCGC CTTTTGGAGTCCCCAGGGTCTGTCATTTCCATGGTCATGTCCATGTGTACCCATTGTTTAGCTCCCAAGA AAAGACTTTTAAAGATAATTTTAAAAACCAAGCTTTGTTTCCTCTGTTTCCAGCTTGGTTCATTCCATAG CACATCTCATCTTCTGAGTCTTGGTCTCGCGTTTAGCATTCGTGATGGTTTATGTCGTTGGGGTGATGCA GAAACCAGCCTTTTTTTGATATCGGTATTTAACACGTGCTTGACAGTTGACACCTTACCTGCTCTGTTGG GACTTGCTTTGCTCACTTTGTTTTTTGCCGTCGTCTTCTGTGCTTGTTTGAAAAGCTGTTTGAAGAGGTA AGGGGTGGGCCATTCCGTTGTGAACACTCTGAGCTGGTGTGCATGTGGCCCCTGCACAGCAGCCCTGGCC GAGCTTGGCTGTGCTCCTGGCACTCAGAGTCAGGTAGCTGAGCACTCGCTCCCTTTTCTTCTCCCTGAAA CTTCAAATATTTTCTTAAAGCAATTATTTTTTACTTATCTGTCATTTAATGACAACCTTAGTTTCCTCCT CTCTTTCCAGTTAGCTGTGCCAGGGGATAGGTCTGTCCTAGGGAGCAGCTGCCCCCTCCTCTCCTCCTCC CTCAGGAGCGTGCAGGATGTCTGTGCACACCAGCGCCAGCCTGCCTCCTGGTGACACCTCTCATCACAGG ACTAACAGGACCCAAATGACACGAGGGGTTTTATTAGAATT 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK093579 unedited GGGGTTCAAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGTGTGTGT GAGGCCATCGCTCAGCATCTGCACGCTGTCTGCTGCGTCTAACGACCCCTCCTCTAGGTT GGGGATCCTGTCCAGGAGCCCTGCATTTTGTCATCCAGAAGCCATTTGAGATGAAGAGAA CATTTACTAATACAAATGAAAAACAATCACTTAGGCGGTTTCCCCCTTTGATGCATAAGA AATCATGATGCGGTCTTTGCTTGTCTCTGTTCTCATCCGGCATTTTTATATTTGCTAGGC GCAAATAAAGTACGATTCCAATGAATAAATAGAAGTGTTAACACTTCCTTACACTTTTAC CTAAACCAGCTTGGATGAGGAAAGGAAGTGTCTTTAGAAATCTGTTCTGAGAAGTACTTT CACCTGAGGAAGCTGATAATTGAGATGAGAGTTGTTTTCATAAGCAGTTGAGCGTGCATG GTGGTGAGGTATGAGGAGTGGCGGTGAGAGTGTGGGTATCGTGCGCGTCAGTGTGAGCAG CAGTGCCCGTTGGTGGGGTGTGAGTGAGCGTGAGGATGGTGACACGCTCTTGCCTGGCAT TGTGCCTCTGTGTCTGGTCAAGGCCACATTTGCCAAGGGAACTGGAGCAGACTTTTTCTA CATTCTTGCCGGCAGCCCTGGGGGTTTTAAGGCCCACAGGAAAGTACTGTATGAGAAATT AGGATCTGTGCTTTTCATTTTTTGTTCTGAGTCTTTGTTTTTATGATATCTTTCAGGTAA TCATGTGTGCAAGTCAGAATTTCCATTTTCTATCATGGGAAGTTACTTGAAGAATGNNTG CTGTNCTTATTGTATACTTTAGTGTGCACAGTCTAAAAAAAAGTGAGCTACTACAGNAAC TGGTGGAGACTGAATTACTAGGNNAGAC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK093579 unedited TCTATTCAGNACGCGGCCGCTTTCTANGATCGGTTTTTTTTTTTTTTTTTTAATTCTAAT AAAACCCCTCGTGTCATTTGGGTGCGGGTAGTCCTGTGATGAGAGGTGTCACCAGGAGGC AGGCTGGCGCTGGTGTGCACAGACATCCTGCACGCTCCTGAGGGAGGAGGAGAGGAGGGG GCAGCTGCTCCCTAGGACAGACCTATCCCCTGGCACAGCTAACTGGAAAGAGAGGAGGAA ACTGAGGGTGTCATTAAATGACAGATAAGTAAAAAATAATTGCTTTAAGAAAATATTTGA AGTTTCAGGGAGAAGAAAAGGGAGCGAGTGCTCAGCTACCTGACTCTGAGTGCCAGGAGC ACAGCCAAGCTCGGCCAGGGCTGCTGTGCAGGGGCCACATGCACACCAGCTCAGAGTGTT CACAACGGAATGGCCCACCCCTTACCTCTTCAAACAGCTTTTCAAACAAGCACAGAAGAC GACGGCAAAAAACAAAGTGAGCAAAGCAAGTCCCAACAGAGCAGGTAAGGTGTCAACTGT CAAGCACGTGTTAAATACCGATATCAAAAAAAGGCTGGTTTCTGCATCACCCCAACGACA TAAACCATCACGAATGCTAAACGCGAGACCAAGACTCAAAAGATGAGATGTGCTATGGAA TGAACCAGGCTGGANACAGAGGAAACAAAGCCTGGGTTTTTAAATTATCTTTAAAAGTCT TTTCTTGGGAGCTAAACATGGGTACACTGGACATGACCATGGAATGACAGACCCTGGGGA CTCAAAGGCANAGGTGATGGGGGTGAGTTTTGAAAAGCTCCCATTGGGTACATGGTTCCT ATTTGGGTATAAGTACCCCAGAAGCCCAAACCCAGCATCACCAATACAGCCCCGTGACAA CT
Restriction Sites	NotI-NotI
ACCN	AK093579
Insert Size	2500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK093579.1
RefSeq Size	2141 bp
RefSeq ORF	2141 bp
Locus ID	22832
Gene Summary	Required to promote assembly of the transition zone in primary cilia. Acts by specifically recognizing and binding the axonemal microtubule. Localizes to the distal ends of centrioles before ciliogenesis and directly binds to axonemal microtubule, thereby promoting and restricting transition zone formation specifically at the cilia base. Required to mediate CEP290 association with microtubules.[UniProtKB/Swiss-Prot Function]

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