CSNK2A2 (AK090752) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CK2A2; CK2alpha'; CSNK2A1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK090752, the custom clone sequence may differ by one or more nucleotides AAAAAAAAAATATATCTTAAACAGGTTAAAAGTGCCTATGATAAGGACTTAATTATTTCCTAAAGAAAAG CAAAAAATGTGGGGAACAGGGTAGTGTTATACTATCATCTCAGGTACTGCCTATGTTCAAGAACATAGAC CTGGTATGGTGAGAACAAAAGGTTCCTATGCCAAATCCAGATGGCTATATCATTTGTTGGCTACAAGTGA GCCTGACTGCAAGGTTTGCTTCTGCTGAGAAATCCCATGGTGAGAGTAGAACTCATGAAGTGCTGAGTCA CATTTGCACCTCTGGTGTGGTGGCAGTTCACTCTCCCCTGGATGCTGTCAGCTGGCCTTGCTTTCATCCT ATCCCTGTAGGCAGGGTAGGTGGCTGGGTAGGAGAAGCTATTGAGTGTATGACACAGACCAAGCTTTCCT GGGCCTTCTCACCAACTAGAGAAGCGCTGATGTCGTTCATTGAGGCACTTAAACACCAGTCACTCAGCCA GGCCTCCTCCCAGACATTCTGTAACATGTCAGCACTCACAAGGTTTAAAGCAAATGTGTTCCCTTGCAGA GAGGAAGGTGCTCTCAGACTGATAGATTAGAGGAGACAGACAAACTCGTAATCCTTGGGGACTTGAAGAG AGTGGCTAGGGGAAGGGCTGTTAAAAGAAAAGGGACCAATAAGAAAATCCATCTAAAGGTAGCTCTTGAG GAGGAAATTAACTTCTTGAGGGGATAAATTAGAGGGTACCAAGTTTATGTTCTGGAATGTACCCTGGGGA GGGCGCTGCCCACTGGATTGGTCCCAGTGGACTGAAAGGTGGTGCTCTCGAGTCAGGCGCTTAGTCCCAG AGAGAGCTGGGTGCCGCGGGGCTCGTGGTCTGTGCCTACTCAGGGAAATGACTTAGGTTCTCCCAGGTGC TCTGTTGATAAGGATGGAAGCAGAACTACTTTGAGACACAAGCAACAATTGGAAGAGTTTTTTTATATGA ACTGAAATAGATCTAAGAGAGCCTGGTTAACAGTTTCCTTTAATACCAGTTCAGATTAAATGGTTACTTT AGGAACCTGTGTTACTGTGTGAGCACCCACAGAAACAAGCAAACTTATTTCCTACAAAAAATGAGGGGAT AATAACCGATCAGAGTCACAGTCGTTTCAATATAAACCCTTCCGAAAGCAAAAGCAGGGACCACATGAGC ACACAAGAAGCCAAAGTTTGTCAGTGATCTTAATAAATTAAGACTGAGTAACATCCTGCCTTTAGAAAAA AATAACCCCGAGGTGGAGACCAGGGCAGGGGAGGTGGCGGAGAAGCATCGGGCCAGATCAACCGTTAATA ACATGCTTCTGAGCTATGTGGATGTATAAATGAAAACACCCCCAACTAGGTATTGTAGAGGCTGGAAAAA GATGTGATGGGATTATACAGGTGATGCATATTTTTGTTTTGAGACTCAACAATGTGCTACAAATGTGATA CTTAACCCTGAACTGCATGTATTGGGGATTGTTTTTTAAAAAGAAAAAAAACAACTTTGAATCAATAAAT TAACAGTTTTTCACTTGCTGGCTCTTCTTGCTGTAAATGGTGACTAAGAGGTTGGGACAGAAATCTTGAC AACTAGTGGAAAACTTGGATAATCTTCAAGGACCATGGAGCCTTCTGTTTCCTTCTGTTTGCAGAAAAGC AACACTGCCTGGGTTGTGATCCTTTACAAGTGACTACTTCAGACACAATTGGCCACCTCTTTGTATCACT GGATGACTGATGTATGATTTATGTGCTGCTGCCCTTAGAGGGAAGAAAAATGCCATGTTAAATCCTGTTT TCTTAAATAAAAATGTTGACAATAACCAGCCTTCCCAAGTGGGAGACAAACCATT 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK090752 unedited NNNAGGGGGTTCACCATTTGTNATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGG TTAGACTAAACCATGGTAAATGTACGCACCCAGTATCCTTTTCAGTTAGCATATTCTTTC TTAAACTCATCTCAGTACTTTTCATTTGTTTGCTTCATTATCTTAAACAGGTTAAAAGTG CCTATGATAAGGACTTAATTATTTCCTAAAGAAAACAAAAATGTGGGGAACAGGGTAGTG TTATACTATCATCTCAGGTACTGCCTATGTTCAAGAACATAGACCTGGTATGGTGAGAAC AAAAGGTTCCTATGCCAAATCCAGATGGCTATATCATTTGTTGGCTACAAGTGAGCCTGA CTGCAAGGTTTGCTTCTGCTGAGAAATCCCATGGTGAGAGTAGAACTCATGAAGTGCTGA GTCACATTTGCACCTCTGGTGTGGTGGCAGTTCACTCTCCCCTGGATGCTGTCAGCTGGC CTTGCTTTCATCCTATCCCTGTAGGCAGGGTAGGTGGCTGGGTAGGAGAAGCTATTGAGT GTATGACGCAGACCAAGCTTTCCTGGGCCTTCTCACCAACTAGAGAAGCGCTGATGTCGT TCATTGANGCACTTAAACACCAGTCACTCAGCCAGGCCTCCTCCCAGACATTCTGTAACA TGTCAGCACTCACANAGTTTAAAGCANATGTGTTCTCTTGCAGAGAGGAAGGTGCTCTCA GACTGATAGATTAGAGGAGACAGACAAACTCGTAATCCTTGNGGACTTGAAGAAGTGGCT AGGGGAAGGGCTGTTAAAAGAAAGGGACCAANTAGAAATCCATCTAAAGTAGCTCTTGAN GGAGAAATAAACTTCTTGAGGGATAAATTAGAGGNTACCAAGTTTATGTTCTG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK090752 unedited TTTAAGGTTCACTATGNACGCGGCCGCATACTAAGATCGGTTTTTTTTTTTTTTTTTTGA AAAACTGTTAATTTATTGATTCAAATTGTTTTTTTTCTTTTTAAAAAACAATCCCCAATA CATGCAGTTCAGGGTTAAGTATCACATTTGTAGCACATTGTTGAGTCTCAAAACAAAAAT ATGCATCACCTGTATAATCCCATCACATCTTTTTCCAGCCTCTACAATACCTAGTTGGGG GTGTTTTCATTTATACATCCACATAGCTCAAAAGCATGTTATTAACGGTTGATCTGGCCC GATGCTTCTCCGCCACCTCCCCTGCCCTGGTCTCCACCTCGGGGTTATTTTTTTCTAAAG GCAGGATGTTACTCAGTCTTAATTTATTAAGATCACTGACAAACTTTGGCTTCTTGTGTG CTCATGTGGTCCCTGCTTTTGCTTTCGGAAGGGTTTATATTGAAACGACTGTGACTCTGA TCGGTTATTATCCCCTCATTTTTTGTAGGAAATAAGTTTGCTTGTTTCTGTGGGTGCTCA CACAGTAACACAGGTTCCTAAAGTAACCATTTAATCTGAACTGGTATTAAAGGAAACTGT TAACCGGGCTCTCTTAGATCTATTTCAGTTCATATAAAAAAACTCTTCCAATTGTTGCTT GTGTCTCAAAGTAGTTCTGCTTCCATCCTTATCAACAGAGCACCTGGGAGAACCTAAGTC ATTTCCCTGAGTANGCACAGACCACGAGCCCCGCGGCACCCAGCTCTCTCTGGGACTAAG CGCCTGACTCGAGAGCACCACCTTTCAGTCCACTGGGACCAATCCAGTGGGCAGCGCCCT CCCCAGGTACATTCCAGAACT
Restriction Sites	NotI-NotI
ACCN	AK090752
Insert Size	1750 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK090752.1
RefSeq Size	1875 bp
RefSeq ORF	1875 bp
Locus ID	1459
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Adherens junction, Tight junction, Wnt signaling pathway
Gene Summary	This gene encodes the alpha', or alpha 2, catalytic subunit of the protein kinase enzyme, casein kinase 2 (CK2). Casein kinase 2 is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. It is involved in various cellular processes, including cell cycle control, apoptosis, and circadian rhythms. This heterotetrameric kinase includes two catalytic subunits, either alpha or alpha', and two regulatory beta subunits. The closely related gene paralog encoding the alpha, or alpha 1 subunit (CSNK2A1, Gene ID: 1457) is found on chromosome 20. An intronic variant in this gene (alpha 2) may be associated with leukocyte telomere length in a South Asian population. A related transcribed pseudogene is found on chromosome 11. [provided by RefSeq, Aug 2017]

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