MBOAT1 (AK056323) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HCCS1; hVps53L; PCH2E; pp13624
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK056323, the custom clone sequence may differ by one or more nucleotides GGGATTTCGCCACTGCACTCCAGCCTGGGTGACGGAGCGAGACCCCTTCTCAAAAAGTTTTCAGGATAGA GGAAATTTCCTTAAAATCCATAAAACTTGTAACAGCCGTACAGTAGGAATAAGGTTTGGAAATCTGCCTG GAAACCTGATTTCCTCATCCCCACCTTGTTTCCCAACATAACCACTGCTCTGTGCCCCTGCCCTCTCTGC CAGGCTTTCTCCCCGATGCCGTCCAGCTCTGATCCTGAGTGTCATTTCTTCATCGGCTTCCAGGCTGTCC TTGCTAGCTGGTTTGACTCTCACGCATCAAGCGTCTCAAATTTGAGAATGCGGAGGAGAAAAGAAGTGTT GCGAGAAGGAGGTGCGGCCTTCAGTTGAGGTCTATTAGGAAAAGATTCCAAATAAGACAAGGGCGGGGGG CGGCAGTCGGGAGAGCCCCCAGGAAGCCCTGTAGATGCCCCCACCCCAGCCCATGGAGTTGCTATGGTTA AGCAGCCTGAGCCGTACAGTTGAAAAGGGGCGGTGGGGCCCATCTCCAAATAGCACAGCCAGTTCAGCCG TCTGTCCTCAACCCAGGAGAGCTGGGAAAGCCAGTGGACTCCTGCACCTGGTTCTTGTCACTCCTGGCGT TTGTGTACAGATCACCACTTCTCTTTTTGGTTCATTGTTGAGGAGTCGTTTTCCGGACGGCTCCATCACA GCATTGAGCTATCAAGCTGCTGTTTTCCACAGTTGGTGGTCACAGCCAGGGAAGTTGCTGTCTTGATTTG TTACATTGTCAGTAATCCCCAAGGAACCAATGGATGTCAGTAGGAGTTTCTGTTAAATGTCTCCTTGATG GGGACTCAGTACTGTGTAGAGACGCTGTGTTTCTCTTCTGGGGGTGTGCATCAGAACCACTGGGGCCTTT TAAAATCTACAGATGCCGGCCGGGCGCCGTGGCTCACGCCTGGAATCCCAGCACTTGGGGAGGCTGAGGC GGGCGGATCACAAGCGCAGGAAATTGAGACCATCCTTGCCAATATGGTGAAACCCCATCTCTACAAAAAA TACAAAAATTACCGGGGTGTGGTGGCGTGCACACCTCCCAGCTACTTGGGAGGCTGAGGCGGGAGAATCG CTTGAACCCGGGAGGCGAAGATTGCAGTGAGCCGAGATCGCGCCACTGCACTCCAGCCTGGCGACAGAGT GAGACTCTGTCTCAAAACAAACAAAAAAACAGATGCCCGGAGATTTTGAGTCAGTAAGTTTGGATGGGGC CTGGCATACAGGTTTAAAAGTTCCATAGGTAGTTATAACGTTTACACCTTGTTAAGAACCACTGCAACAG AGAGATTTGCTTTATGGAAGGGCTCCTGCCACGCATTTTCATACATGTCTATTTTTCTGACTCTAAGCTA AAAGTGCGCCGACACTACACTTGAGCTGGTGCCAACGTGGTGAAGATTCTCTACTGATAACAGCTTTCAT TTTAAACTTGTGTTGGGTCAGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGTGG GCGGATCACATGAGGTCACGAGTTTGAGACCAGCCTGGCCAACATGGCAAAATCCCATCTCTACTAAAAA TACAAAAAAAAAAAAAAAAAAAGCAGGCAGGTGGCGGGTGCCTGTGGTCCCAGCTACTTGGGAGGCTGAG GCAGGGGAATCAGTTGAATCCGGGAGGCGGAGGTTGCAGTGGGCCGGGGTCCCGCCACTGCACTCCAGCC TGGGAGACAGAGTGAGACTCAGTT 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK056323 unedited GCATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGACAGAGCGAGA CCCCTTCTCAAAAAGTTTTCAGGATAGAGGAAATTTCCTTAAAATCCATAAAACTTGTAA CAGCCGTACAGTAGGAATAAGGTTTGGAAATCTGCCTGGAAACCTGATTTCCTCATCCCC ACCTTGTTTCCCAACATAACCACTGCTCTGTGCCCCTGCCCTCTCTGCCAGGCTTTCTCC CCGATGCCGTCCAGCTCTGATCCTGAGTGTCATTTCTTCATCGGCTTCCAGGCTGTCCTT GCTAGCTGGTTTGACTCTCACGCATCAAGCGTCTCAAATTTGAGAATGCGGAGGAGAAAA GAAGTGTTGCGAGAAGGAGGTGCGGCCTTCAGTTGAGGTCTATTAGGAAAAGATTCCAAA TAAGACAAGGGCGGGGGGCGGCAGTCGGGAGAGCCCCCAGGAAGCCCTGTAGATGCCCCC ACCCCAGCCCATGGAGTTGCTATGGTTAAGCAGCCTGAGCCGTACAGTTGAAAAGGGGCG GTGGGGCCCATCTCCAAATAGCACAGCCAGTTCAGCCGTCTGTCCTCAACCCAGGAGAGC TGGGAAAGCCAGTGGACTCCTGCACCTGGTTCTTGTCACTCCTGGCGTTTGTGTACAGAT CACCACTTCTCTTTTTGGTTCATTGTTGAGGAGTCGTTTTCCGGACGGCTCCATCACAGC ATTGAGCTATCAAGCTGCTGTTTTCCACAGTTGGTGGTCACAGCCAGGGAAAGTGCTGTC TTGATTTGTTACATTGTCAGTAATCCCCAGGAACCAATGGATGTCAGTANNGAGTTCTTG TAATGTCTNCTTGATGGNGACTCANTACTGTGTAGAGACGCTGTGTTNCTCTCTGGNGGT GTGCATCAGAACCACTGGGCCTTTTAAATCTACGATGCNGNCGGGGCGCGTGGCTACGCC TGAATCCAGACTTGGNGAGCTGNAGCGGCGGATACAGCGCAGAATGAAA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK056323 unedited GGTACACTATGNACCGCGCCGCAATCTATATCGAGTTTTTTTTTTTTTTTTTTGTATTTT TAGTAGAGATGGGATTTTGCCATGTTGGCCAGGCTGGTCTCAAACTCGTGACCTCATGTG ATCCGCCCACCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCGCGCCTGAC CCAACACAAGTTTAAAATGAAAGCTGTTATCAGTAGAGAATCTTCACCACGTTGGCACCA GCTCAAGTGTAGTGTCGGCGCACTTTTAGCTTATAGTCAGAAAAATAGACATGTATGAAA ATGCGTGGCAGGAGCCCTTCCATAAAGCAAATCTCTCTGTTGCAGTGGTTCTTAACAAGG TGTAAACGTTATAACTACCTATGGAACTTTTAAACCTGTATGCCAGGCCCCATCCAAACT TACTGACTCAAAATCTCCGGGCATCTGTTTTTTTGTTTGTTTTGAGATAGAGTCTCACTC TGTCGCCAGCTGGGAGTGCANTGGCGTGATCTCGGCTCACTGCAATCTTTGCCTCCCGGG TTCAAGCGATTCTCCTGCCCCAGCCTCCCAAGTAGCTGGGAGGTGTGCACGCCACCACAC CCCGGTAATTTTTGTATTTTTTGTAAAGATGGGGTTTCACCATATTGGCAAGGATGGTCT CAATTTCCTGCGCTTGTGATCCGGCCGACTCAGCCTCCCCAGTGCTGGGATTCCAGGCCT GAGCCAAGGGCCCGGGCCGCATCTGTAGATTTTAAAAGGCCCCGTGGGTCTTATGCACAC CCCCAGAGAGAAACACAACGTCTCTCCCAGTACTGGGTCCCCTTCAGGGGACCTTTACAC AAACTCCACTTGCCTCTTTGTTTCCTGGGGGATTCCGGAAATGGAACAA
Restriction Sites	NotI-NotI
ACCN	AK056323
Insert Size	1750 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK056323.1
RefSeq Size	1774 bp
RefSeq ORF	1774 bp
Locus ID	154141
Protein Families	Transmembrane
Gene Summary	This gene belongs to the membrane-bound O-acetyltransferase superfamily. The encoded transmembrane protein is an enzyme that transfers organic compounds, preferably from oleoyl-CoA, to hydroxyl groups of protein targets in membranes. A translocation disrupting this gene may be associated with brachydactyly syndactyly syndrome. Alternately spliced transcript variants have been described for this gene. [provided by RefSeq, Nov 2012]

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