FAM119A (METTL21A) (AK094485) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK094485, the custom clone sequence may differ by one or more nucleotides AAGCAACCCAGAGCCCTCCTCCCGCCTGCTCTGTGTAGGAGACAGCCTGCGGAGCCCACTGCCGGCGCGC AGAGAGCCCAGCAGCTCTTGAGTCCAGGAAGCAAGGGTGGGAGGAGGGACGAGCCAGTGGCAATGACCCA GACCCGGACGACCCCCATTGCTGAGCCAGCGGAGCTGCTTGGTGAGTGGCCTCCAGGCGGCATCTCTTGC TCTCAGCCCGGAGGGGGTTTTGGGGACCCAGGCTGTGGGGCTGCCTCTTCCCATGCCTTCAGTTCACCCC GATATGGGGTGAAAAACCCTAGAGACCCTCGGGAGTCGGTCCACCAAGGGAGGTCCAGACTCCCCAGGAA TGTTGCCTGGGGAGGGGTGTCGCGGTGGGGGGGGTGACCCAGGCCCACCCTCTGCAGGGCACTTAAAATG CTCCACATCCCCAGTGGATGCCTCCTGTCACGCATCACACCGTGGTGTATTTGAGATCCCATCTGCCACT TGGGCTGGAGGGAAATGGATGTTTTTGGGTTTCTTGTCATTGCTTCCTGCGGATTCCATTTTACTCCTGT TCGTTTATGGCCTCAAACATGAATTTGAAGAATTTTGCCCTCCCTGGCAGTATTGGGAAGAAGATTCTTT TGTTCAAGTCCGTTTGGACAATTAGATGGGAGTATTAACAAAAGGTCGGGCTGGGCACAGTGGCTCACAC CTGTAATCCCAACACTTTGGGAGGCCAAAGAGCAAGGATCGCTTGAGCCCAGGAGTTCGAAACCAGCTGG GGCAACAGAGGGAGAACTTGTCTCTACAAAAAATTTAAAAATCAGCTGGGCATGGTGGTGCGCGCCTGTA GCCCAAGCTATATGGGAAGCTGAGGTGGGAGGATTGCTTGAGCCCGAGAGTTTGAGGCTGCTGTGAGCTG AGATCACGCCACTGCATTCCAGCCTGGGCAACAAAGCAAGACCCTGTCTGAGGTCAACTCTGCCCCCAGG AAGGTATGGGACCTTGCAATCTTATCTAGGTTGCGTCTTTTTCATTGAAATCACGAAATCATTAAGGTAA GTAGGGAAGGGTCAAGATAGCTAAGATGAGTGAGGGGACACAGACACCAATAAAAGTTCTAACTTTGAGG ACTCCTCCAGTATTCTTTTCATCCTAAAGCTTCTGGCCTATCAGCTAGTTCAGACACAGCCTAGGAGTCT CAAGCCAGTACAGGAACAAGGAGGCAGGTTGCCCTCTTGGGGCAAAGCTGTGGCCCCACCCTTGATGAAG AACGCCTGTAGGAACCCAGGACCTGTGAGCAGGTAGAGTCCTGAAGGTTTAAGATTCTGTGGAGCAGGTA TGTGCAGTGCCAGTGCGCAACCAGCAACTGATGGACAGGAATAGGTAGAGCTGCTCTGAGTGCATTCCTT TGGCAAGCCTGGGCCAGAGGCACACAGGAAGGAATTAGTCATGTGGTATCATAGGCCAGGTGTGGAAGCA AATGAGCCAGAGTGGAACCAGGACCAGCCAGGGCCAAGGCAGGGAGAAGAGTTCCTCTAGCTGACATTGA CAGGAAAGCAGAGTCAAAAACCTGGAATGGTTTGCTTCAAAGACTGGATGCTGCCTAATGACCTCGAAGG CCAGTGATACCTGGACTCTTGAGTGAGCAAGGCAGATTTTGGAATGAGCACACAACTTCAAGTAAGCCCG GAGATGATTACCTGTAGTTTCTGCTGCTCTTAGGGGCAGAATCATCTGAGAACTGATTGAGGAGTGCATG TATACCCCAGAGACTAGTGCACTCTAGGATAAAAGGAGTCATTCAGCCCTTACTTTTGGTTGGATGCAGG GTGTAGTTTCAATAAAATGTCACAGAACAAATTTCTAAGACCTTTAGGTTGAGTGAGTGTTAGAGTTTGT CTAGTAGTATATTTCTCATCAGTTACCTATTTAGCTGTTCTGCATAGGCCTTTGAGATAAGGAAATAAGT AAGATAAATTCTCCCCAGAAACCGCAAAACAATTATAATGTTGAGAGAAGAATGAAGTAGTAACTCTGTC TGTACTGAAGAAGGAATTCGGTGTACAGAAAAGAGGTCACTGCCTGAGGTCGGGAGTTCAAGATCAGCCT GGCCAACATGGTGAAACCCCATCTCTACAAAAATACAGAAATTAGTCGGGCATGATGGTGGGTGCCTGTA ATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATTGCTGGAACCTGGGAGGTGGAAGTTGCAGTGAGCCG AGACTGTGCCATTGCACTCTGGCCTGGGCAACTGAGCGAGACTCCGTCTC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK094485 unedited GTTCAAATTTTGTAATACGACTCACTATAGGGCGGCCCGCGAATTCGCACGAGGCCCTCG GGTGTCGGTCCACCAAGGAGGTCCAGACTCCCCAGGAATGTTGCCTGGGGAGGGGTGTCG CGGTGGGGGGGGGGGTGACCCAGGCCCACCCTCTGCAGGGCACTTAAAATGCTCCACATC CCCAGTGGATGCCTCCTGTCACGCATCACACCGTGGTGTATTTGAGATCCCATCTGCCAC TTGGGCTGGAGGGAAATGAATGTTTTTGGGTTTCTTGTCATTGCTTCCTGCGGATTCCAT TTTACTCCTGTTCGTTTATGGCCTCAAACATGAATTTGAAGAATTTTGCCCTCCCTGGCA GTATTGGGAAGAAGATTCTTTTGTTCAAGTCCGTTTGGACAATTAGATGGGAGTATTAAC AAAAGGTCGGGCTGGGCACAGTGGCTCACACCTGTAATCCCAACACTTTGGGAGGCCAAA GAGCAAGGATCGCTTGAGCCCAGGAGTTTGAAACCAGCTGGGGCAACAGAGGGAGAACTT GTCTCTACAAAAAATTTAAAAATCAGCTGGGCATGGTGGTGCACGCCTGTAGCCCAAGCT ATATGGGAGGCTGAGGTGGGAGGATTGCTTGAGCCCGAGAGTTTGAGGCTGCTGTGAGCT GAGATCACGTCACTGCATTCCAGCCTGGGCAACAAAGCAAGACCCTGTCTGANGTCAACT CTGCCCCCAGGAAGGTATGGGACCTTGCAATCTTATCTANGTTGCGTCTTTTTCATTGAA ATCACGAAATCATTAANGTAAGTANGGGAAAGGTCAAGATAGCTAAGATGAGTGAGGGGA CACAGACACCAATAAAAGGTCTAANTNTGAAGACTNCTNCCATATTCTTTTTATTCTAAA ACTTCTGGCCTATCANCTTATTCAAACACAGCCTATGAGTCTCAAGCCAGTCCAGGACCA AGGAGGC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK094485 unedited TTTTAAATCACTATGNAACGCGGCCCGCTANCTAGAGTCGAGTTTTTCTTTTTTTTTTTT TTTTTTTTTTTTTTGAGACGGAGTCTCGCTCAATTGCCCAGGCCAGAGATGCAATGGCAC AGTCCCGGCTCACTGCAACCTCCACCTCCCAGGTTCCAGCAACTCTCCTGCCTCAACCTC CCGAGCAGCTGGGATCACAGGCACCCACCATCATGCCCGATCTATTTCTGTATTTTTGTA GAGAAAGGGGGTTCACCATGTTGGCCANGCTGATCTTGAACTCCTGACCTCAGGCAGTGA CCTCTTTTCTGTACACCGAATTCCTTCTTCAGTACAGACAGAGTTACTACTTCATTCTTC TCTCAACATTATAATTGTTTTGCGGTTTCTGGGGAGAATTTATCTTACTTATTTCCTTAT CTCAAAGGCCTATGCAGAACAGCTAAATAGGTAACTGATGAGAAATATACTACTAGACAA ACTCTAACACTCACTCAACCTAAAGGTCTTAAAAATTTGTTCTGTGACATTTTATTGAAA CTACACCCTGCATCCCACCAAAAGTAAGGGCTGAATGACTCCTTTTATCCTAGAGTGCAC TAGTCTCTGGGGTATACATGCACTCCTCAATCAGTTCTCAAATGATTCTGCCTCTAAGAA CAGCAAAAACTACAGGTAATCATCTCCGGGCTTACTTGAAGTTGTGTGCTCATTCCAAAA TCTGCCTTGGTCACTTCAGAGTCCAAGTATCACTGGGCTTCGAGGGCATTAGGCAGCATC CATTCTTTGAAACAAACCATTCAGGTTTTTTACTCTGACTTCCCGTGCAGGGCAGCTAGA AGAACTCTTCTCCCTGCCTTGGCCCTGGCTGGGCCCGGGTCCCCTCCGGTCA
Restriction Sites	NotI-NotI
ACCN	AK094485
Insert Size	2250 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK094485.1
RefSeq Size	2290 bp
RefSeq ORF	2290 bp
Locus ID	151194
Gene Summary	Protein-lysine methyltransferase that selectively trimethylates residues in heat shock protein 70 (HSP70) family members. Contributes to the in vivo trimethylation of Lys residues in HSPA1 and HSPA8. In vitro methylates 'Lys-561' in HSPA1, 'Lys-564' in HSPA2, 'Lys-585' in HSPA5, 'Lys-563' in HSPA6 and 'Lys-561' in HSPA8.[UniProtKB/Swiss-Prot Function]

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