Prickle (PRICKLE1) (NM_153026) Human Untagged Clone

CAT#: SC101342

PRICKLE1 (untagged)-Human prickle homolog 1 (Drosophila) (PRICKLE1), transcript variant 1



  "NM_153026" in other vectors (6)

CNY 6,096.00

CNY 7,220.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal PRICKLE1 Antibody
    • 100 ug

CNY 5,808.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms EPM1B; RILP
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC101342 sequence for NM_153026 edited (data generated by NextGen Sequencing)
ATGCCTTTGGAGATGGAGCCCAAGATGAGCAAACTGGCCTTTGGCTGTCAGAGAAGTTCC
ACATCAGATGATGACTCTGGCTGTGCATTGGAGGAGTACGCCTGGGTCCCCCCGGGCCTG
AGACCAGAGCAGATCCAGCTCTATTTTGCTTGCTTACCAGAGGAAAAAGTTCCTTACGTT
AACAGCCCCGGAGAGAAGCATCGGATTAAACAGCTTTTGTACCAGTTACCACCACATGAT
AATGAGGTACGGTATTGCCAGTCTTTGAGTGAAGAGGAGAAAAAAGAGTTGCAGGTGTTC
AGTGCTCAGCGGAAGAAAGAAGCACTGGGAAGAGGAACAATTAAGCTTCTGTCCAGAGCA
GTCATGCATGCTGTGTGTGAGCAGTGTGGTTTGAAGATAAATGGAGGTGAAGTTGCAGTG
TTCGCCTCCCGTGCGGGCCCTGGTGTGTGCTGGCACCCATCCTGTTTTGTCTGTTTCACG
TGTAATGAGCTGCTGGTCGACCTCATCTATTTTTATCAGGATGGAAAAATTCACTGTGGC
AGGCACCATGCAGAACTGCTCAAACCACGGTGCTCAGCATGTGACGAGATAATTTTTGCT
GATGAGTGCACAGAAGCTGAGGGTCGCCATTGGCACATGAAACACTTCTGCTGCCTTGAG
TGTGAAACGGTCCTGGGAGGACAGAGGTATATCATGAAGGACGGCCGCCCCTTCTGCTGT
GGCTGTTTTGAGTCTCTCTATGCGGAGTACTGTGAAACCTGTGGGGAACATATTGGTGTG
GACCATGCACAGATGACCTATGACGGGCAGCACTGGCACGCCACGGAAGCCTGCTTTTCT
TGTGCCCAGTGTAAAGCCTCTTTGTTGGGATGTCCCTTCCTTCCCAAACAGGGTCAGATT
TACTGCTCAAAAACGTGCAGTCTTGGTGAAGACGTCCATGCCTCTGATTCTTCCGACTCT
GCATTTCAGTCAGCTCGATCAAGAGACTCCCGAAGAAGTGTCCGAATGGGCAAGAGCAGC
CGGTCAGCAGATCAGTGTAGACAGTCTCTCCTCTTATCGCCTGCTCTGAACTACAAGTTT
CCTGGCCTCTCAGGCAATGCTGATGACACCCTTTCTCGAAAATTGGATGATCTGAGTCTC
TCCAGACAAGGAACAAGTTTTGCCAGTGAAGAATTTTGGAAAGGCAGAGTAGAGCAGGAA
ACTCCAGAAGACCCTGAAGAATGGGCTGATCATGAAGATTATATGACGCAGCTCCTCCTC
AAGTTTGGTGATAAAAGCCTCTTTCAGCCACAGCCCAATGAGATGGATATTCGAGCCAGT
GAGCACTGGATATCTGATAACATGGTTAAAAGTAAGACCGAGTTAAAGCAAAATAACCAG
AGCCTTGCAAGTAAAAAATACCAGTCTGATATGTACTGGGCACAGTCACAAGATGGACTG
GGCGATTCTGCTTATGGCAGCCACCCAGGCCCTGCAAGCAGTAGAAGGCTTCAGGAATTG
GAACTGGACCATGGGGCTTCAGGGTATAATCATGATGAAACACAGTGGTATGAAGATTCC
CTGGAGTGTCTGTCAGACCTGAAACCAGAGCAAAGTGTTCGGGATTCGATGGATTCTTTG
GCATTGTCCAATATCACAGGGGCTTCGGTGGATGGAGAAAACAAGCCAAGGCCATCATTG
TATTCTCTGCAAAATTTTGAGGAGATGGAAACAGAAGATTGTGAGAAGATGAGCAATATG
GGAACTTTGAACTCTTCCATGCTGCACAGGAGTGCAGAGTCCTTAAAGAGTCTAAGTTCA
GAGTTGTGTCCAGAGAAAATCCTGCCTGAAGAGAAGCCAGTACATCTGCCAGTGCTCAGA
AGGTCCAAGTCTCAATCCAGACCCCAGCAGGTCAAGTTTTCTGATGATGTCATTGACAAT
GGGAACTATGACATTGAAATCCGGCAGCCTCCGATGAGTGAAAGGACTCGGAGACGCGTC
TACAATTTTGAAGAGAGGGGATCCAGGTCTCATCACCACCGCCGCCGGAGAAGTAGAAAG
TCCCGCTCCGACAATGCCCTGAATCTTGTTACAGAAAGAAAATACTCTCCCAAGGACAGA
CTGCGGCTGTACACCCCCGATAACTATGAGAAATTTATACAGAATAAAAGTGCCCGGGAG
ATCCAAGCATACATCCAGAATGCTGATCTCTACGGACAGTACGCCCATGCCACTTCCGAT
TATGGCCTGCAGAACCCAGGAATGAATCGGTTTCTGGGACTCTACGGCGAGGATGATGAT
TCCTGGTGTTCTTCCTCCTCCTCCTCTTCCGACTCGGAAGAAGAAGGATATTTTCTTGGA
CAACCAATCCCTCAACCCCGGCCACAGAGATTTGCCTACTATACAGATGACCTTTCTAGT
CCACCATCTGCACTTCCCACCCCTCAGTTTGGTCAGAGGACAACAAAATCCAAGAAGAAA
AAGGGACACAAGGGCAAAAATTGTATTATTTCTTAA

Clone variation with respect to NM_153026.2
>OriGene 5' read for NM_153026 unedited
TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTGCGCTGCTCACCC
TCGCCCACCCCTGACGCCGCGGACCGCAGCTCCTCAGCCCCGCAGCCCCGCAGCCCAGCA
GCCCCGCAGCGGCGCCGAGGACAGTGCAGCCGAGTTGCGTCTCCCTCGCGCGAGCAGCCA
TTGTTTGATGTCCTGTGACTGAGAAACCAGGCTTTAAACTTTATGCCTTTGGAGATGGAG
CCCAAGATGAGCAAACTGGCCTTTGGCTGTCAGAGAAGTTCCACATCAGATGATGACTCT
GGCTGTGCATTGGAGGAGTACGCCTGGGTCCCCCCGGGCCTGAGACCAGAGCAGATCCAG
CTCTATTTTGCTTGCTTACCAGAGGAAAAAGTTCCTTACGTTAACAGCCCCGGAGAGAAG
CATCGGATTAAACAGCTTTTGTACCAGTTACCACCACATGATAATGAGGTACGGTATTGC
CAGTCTTTGAGTGAAGAGGAGAAAAAAGAGTTGCAGGTGTTCAGTGCTCAGCGGAAGAAA
GAAGCACTGGGAAGAGGAACAATTAAGCTTCTGTCCAGAGCAGTCATGCATGCTGTGTGT
GAGCAGTGTGGTTTGAAGATAAATGGAGGTGAAGTTGCAGTGTTCGCCTCCCGTGCGGGC
CCTGGTGTGTGCTGGCACCCATCCTGTTTTGTCTGTTTCACGTGTAATGAGCTGCTGGTC
GACCTCATCTATTTTTATCAGGATGGAAAAATTCACTGTGGCAGGCACCATTGCAGACTG
CTCAAACCACGGTGCTCAGCATGTGACGAGATNNATTTTGCTGATGAGTGACAGAAGCTG
AGGGTCGCCATTGCACATGAAAACTTCTGTGCCTTGAGNNGGAACGTCCTGGAGGNAAGA
GTTTCATGNAAGACCGCCGCCTCTTTC
>OriGene 3' read for NM_153026 unedited
GTACTCTATGNACCGCGCCGCAATCTANGATCAGTTTTTTTTTTTTTTTTTTACTTGTTT
TTACCATATATTCCAGGGGTTATGTGTGTGGAGGGGTTGTTTTTACATTTATACAGTTTA
CATTGCTTGACCCATAAGTAACACTTGTAAGATTAATAGGGCCATAGTTTTAAAGAGTAC
TTACATAGCCCCACCATGCATACAGAGCCTTTTTTAATATTGGAAAAAAAAATCAAAAAA
AGTTGAATTTTCCATCACTAGGCAATGTAAATTTGACCATCTACATTTATAAACCCTGAC
TAAAATATAAGCAATCAGTTCATCCATAAATTCTGGTTCCCTGGCAAATCTAGCACTGCA
GCGTAACAAACGGCTTACAATTCAGGGATATAAAAATATCAGCTTTAAAATCTGAACTGT
ACAGTATATACATTAATATTTGCACCGTTAAATTAGGAATACACTTAAGTCTATGAAATA
CTAAGTTATAAATAGCAATGTTTTTTGTTTTTTTTTTCAATCTGTAGCTGGCGCTGATAC
AATACAATGTTTACCTGGCCAAAGAGGGTTCGAGGGGACAAGCTGGCCTCACAATAAGAT
GCACAGTGTTAGCTAGGTCATCGTGACAGGCATGCCTCACACCAAGACGGACTATCAAGA
CCTGAGCCGACCTGACTTACATAAATGACAAACACTAGTGCTTTACAAAGGTGGCTGGAG
TTCTCCATCTTCTAAAATCACATCCAATCCCCTTTAGTCAGCATCTTCAGTATTCCCTTG
GAGACTGGAAAACCAAGGCGCTACGTCCATCTGTAACGCACCCGACCGGAAAGGCACAAG
ATTGTCCGTCCCCCTGGCACCCTTCAAGAGGGGAAATTGGGGAAAACCAACCTTTAAAGG
TTAATCCGACCCGGTA
Restriction Sites NotI-NotI     
ACCN NM_153026
Insert Size 4000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_153026.1, NP_694571.1
RefSeq Size 3293 bp
RefSeq ORF 2496 bp
Locus ID 144165
UniProt ID Q96MT3
Domains LIM
Protein Families Druggable Genome
Protein Pathways Wnt signaling pathway
Gene Summary This gene encodes a nuclear receptor that may be a negative regulator of the Wnt/beta-catenin signaling pathway. The encoded protein localizes to the nuclear membrane and has been implicated in the nuclear trafficking of the transcription repressors REST/NRSF and REST4. Mutations in this gene have been linked to progressive myoclonus epilepsy. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 3. [provided by RefSeq, Sep 2009]
Transcript Variant: This variant (1) represents the longest transcript. Variants 1-4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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