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PDE8A (NM_002605) Human Untagged Clone
CAT＃: SC101098
PDE8A (untagged)-Human phosphodiesterase 8A (PDE8A), transcript variant 1
CNY 6,080.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HsT19550 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_002605 edited
ATGGGCTGTGCCCCGAGCATCCACATTTCCGAGCGCCTGGTGGCCGAGGACGCGCCTAGC CCCGCGGCACCGCCGCTGTCGTCCGGCGGGCCGCGCCTCCCGCAGGGCCAGAAGACGGCC GCCTTGCCCCGGACCCGCGGCGCCGGCCTCTTGGAGTCGGAGCTTCGCGACGGCAGCGGC AAGAAGGTAGCAGTAGCTGATGTGCAGTTTGGCCCCATGAGATTTCATCAAGATCAACTT CAGGTACTTTTAGTGTTTACCAAAGAAGATAACCAATGTAATGGATTCTGCAGGGCATGT GAAAAAGCAGGGTTTAAGTGTACAGTTACCAAGGAGGCTCAGGCTGTCCTTGCCTGTTTC CTGGACAAACATCATGACATTATCATCATAGACCACAGAAATCCTCGACAGCTGGATGCA GAGGCACTGTGCAGGTCTATCAGATCATCAAAACTCTCAGAAAACACAGTTATTGTTGGT GTAGTACGCAGGGTGGATAGAGAAGAGTTGTCCGTAATGCCTTTCATTTCTGCTGGATTT ACAAGGAGGTATGTAGAAAACCCCAACATCATGGCCTGCTACAATGAACTGCTCCAGCTG GAGTTTGGAGAGGTGCGATCACAACTGAAACTCAGGGCTTGTAACTCAGTATTCACTGCA TTAGAAAACAGTGAAGATGCAATTGAAATTACAAGCGAAGACCGTTTTATACAGTATGCA AATCCTGCATTTGAAACAACAATGGGCTATCAGTCAGGTGAATTAATAGGGAAGGAGTTA GGAGAAGTGCCTATAAATGAAAAAAAGGCTGACTTGCTCGATACTATAAATTCATGCATC AGGATAGGCAAGGAGTGGCAAGGAATTTACTATGCCAAAAAGAAAAACGGAGATAATATA CAACAAAATGTGAAGATAATACCTGTCATTGGACAGGGAGGAAAAATTAGACACTATGTG TCCATTATCAGAGTGTGCAATGGCAACAATAAGGCTGAGAAAATATCCGAATGTGTTCAG TCTGACACTCATACAGATAATCAGACAGGCAAACATAAAGACAGGAGAAAAGGCTCACTA GACGTCAAAGCTGTTGCCTCCCGTGCAACTGAAGTTTCCAGCCAGAGACGACACTCTTCC ATGGCCCGGATACATTCCATGACAATTGAGGCGCCCATCACCAAGGTAATCAATATTATC AATGCTGCCCAGGAAAGTAGTCCCATGCCTGTGACAGAAGCCCTAGACCGTGTGCTGGAA ATTCTAAGAACCACTGAGTTATATTCACCACAGTTTGGTGCTAAAGATGATGATCCCCAT GCCAATGACCTTGTTGGGGGCTTAATGTCTGATGGTTTGCGAAGACTATCAGGGAATGAA TATGTTCTTTCAACAAAAAACACTCAAATGGTTTCAAGCAATATAATCACTCCCATCTCC CTTGATGATGTCCCACCACGGATAGCTCGGGCCATGGAAAATGAGGAATACTGGGACTTT GATATTTTTGAACTGGAGGCTGCCACCCACAATAGGCCTTTGATTTATCTTGGTCTCAAA ATGTTTGCTCGCTTTGGAATCTGTGAATTCTTACACTGCTCCGAGTCAACGCTAAGATCA TGGTTACAAATTATCGAAGCCAATTATCATTCCTCCAATCCCTACCACAATTCTACACAT TCTGCTGATGTGCTTCATGCCACTGCCTATTTTCTCTCCAAGGAGAGGATAAAGGAAACT TTAGATCCAATTGATGAGGTCGCTGCACTCATCGCAGCCACCATTCATGATGTGGATCAC CCTGGGAGAACCAACTCCTTCCTGTGTAATGCTGGAAGTGAGCTGGCCATTTTGTACAAT GACACTGCTGTGCTGGAGAGCCACCATGCGGCCTTGGCCTTCCAGCTGACCACTGGAGAT GATAAATGCAATATATTTAAAAACATGGAGAGGAATGATTATCGGACACTGCGCCAGGGG ATTATCGACATGGTCTTAGCCACAGAAATGACAAAGCACTTTGAGCATGTCAACAAATTT GTCAACAGCATCAACAAACCCTTGGCAACACTAGAAGAAAATGGGGAAACTGATAAAAAC CAGGAAGTGATAAACACTATGCTTAGGACTCCAGAGAACCGGACCCTAATCAAACGAATG CTGATTAAATGTGCTGATGTGTCCAATCCCTGCCGACCCCTGCAGTACTGCATCGAGTGG GCTGCACGCATTTCGGAAGAATATTTTTCTCAGACTGATGAAGAGAAGCAGCAGGGCTTA CCTGTGGTGATGCCAGTGTTTGACAGAAATACCTGCAGCATCCCCAAATCCCAAATCTCT TTCATTGATTACTTCATCACAGACATGTTTGATGCTTGGGATGCCTTTGTAGACCTGCCT GATTTAATGCAGCATCTTGACAACAACTTTAAATACTGGAAAGGACTGGACGAAATGAAG CTGCGGAACCTCCGACCACCTCCTGAATAG >OriGene 5' read for NM_002605 unedited
ATACGACTCACTATAGGGCGGCCGCGATTTGGCACGAGGCATGTTCGGAGGGGCGGCCTC GGCACGCCACCCGCCTAAGCGCCCCCTTCCCACCGCAGCCGCCGCCGCCGCAGCGCCCGC ACCGCGATAAAAGGGGCGGCCGCGTTTCCTGACGCGAGATCCGCGCTCGCCGCCGCCCGC ACAGGCGGCGATGACACGGCGCCCGCGGCGGCCCGGAGGCGCCGGGTGGGCCGTTTGCTG ACCGGATCGCGGCTACCCGCCAGCGTGTCCGCGGCGCCGCCGCCAGCATGGGCTGTGCCC CGAGCATCCACATTTCCGAGCGCCTGGTGGCCGAGGACGCGCCTAGCCCCGCGGCACCGC CGCTGTCGTCCGGCGGGCCGCGCCTCCCGCAGGGCCAGAAGACGGCCGCCTTGCCCCGGA CCCGCGGCGCCGGCCTCTTGGAGTCGGAGCTTCGCGACGGCAGCGGCAAGAAGGTAGCAG TAGCTGATGTGCAGTTTGGCCCCATGAGATTTCATCAAGATCAACTTCAGGTACTTTTAG TGTTTACCAAAGAAGATAACCAATGTAATGGATTCTGCAGGGCATGTGAAAAAGCAGGGT TTAAGTGTACAGTTACCAAGGAGGCTCAGGCTGTCCTTGCCTGTTTCCTGGACANACATC ATGACATTATCATCATAGACCACAGAAATCCTCGACAGCTGGATGCAGAGGCACTGTGCA GGTCTATCAGATCATCAAAACTCTCAGAAACACAGTTATTGTTGGTGTACTACGCAGGGT GGATAGAAAGAAGTGTCCCGTATGCCTTTCATTCTGCTGGATTACAGGNAGGATGTAGAA AACCCCACATCATGGCCTGCTACACTGACTGCTCCACTTGAGTTTGGAGANTGCCATCAC ACTGAACTCAGGCTTGTACTCATATTCCTGCT >OriGene 3' read for NM_002605 unedited
GGGGAGAGCAGGGGGNNNNCNGCNANCCTCCCATCCCTGTTTTTGACTCTGCACNCGCGG CCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTGATATTAATAATCAAGTT CAAATTTTAATTTTTCATCTGCTACTCAATTTTAAAAGAGAAGTCACTCTCAGTAGTGTT CCCAAGTGAGCGATTGCACCTTCTATGATGTGAAAAAGTTGCTAAAGATCCTATTGCCAT TAGCCGTTCTTAAGTGCTGAACGTAACTGAGAGAATTGTTTGCAATAGACATGCTCTGTC ACTGGCAGGAAGTCCATCAATACCCTTTGCAGGGGCACTCCAGACCTTGTGTTTTACCAG AAACTGAGGCAAACCGTGGTCCTCATGTGCCTTTCTGAAGAGCTCCTGCAGCAGGCCCAG GCTACAGTGGCCAAGTCTCACTTGACAGTAATGGCATTGGATGGTGGCTGCAGAAGGTTA CTGATGTCCCCGTGGGCTTTCACAGGCTATGCAGATCGGGGGCTGTTCTGCCTAGTACTG AAAGGACCAAGGAGCTCTGGCTGCCCTCAGGAATTCCAAATGACCGAAGGAACAAAGCTT CAGGGCTCTGGGTGGTGCCTCCCACTATTCAGCAGGTGGTCGGAGGTTCCGCAGCTTCAT TTCGTCCAGTCCTTTCCAGTATTTAAAGTTGTTGTCAAGATGCTGCATTACACCACGCAG GTCTACAAAGGCATTCCCAGCATCAAACATGTCTCGTGATGACGTAATCAATGAAAGAGA ATTGGGATTTGGGGATGCTGCAGGTATTTCTGTCAAACACTGCATCACCCCAGGAAAGCC CTGCTGCTTCTTTTCTCCACCTGAGAAAAAATTCTTCCGAAAGCGTGCACCCCACTCGTG CCGAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002605 |
| Insert Size | 3000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002605.2, NP_002596.1 |
| RefSeq Size | 3931 bp |
| RefSeq ORF | 2490 bp |
| Locus ID | 5151 |
| UniProt ID | O60658 |
| Domains | PDEase |
| Protein Families | Druggable Genome |
| Protein Pathways | Progesterone-mediated oocyte maturation, Purine metabolism |
| Gene Summary | The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE8 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011] Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longest isoform (1). |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209169 | PDE8A (Myc-DDK-tagged)-Human phosphodiesterase 8A (PDE8A), transcript variant 1 |
CNY 6,072.00 |
|
| RC209169L1 | Lenti ORF clone of Human phosphodiesterase 8A (PDE8A), transcript variant 1, Myc-DDK-tagged |
CNY 8,472.00 |
|
| RC209169L2 | Lenti ORF clone of Human phosphodiesterase 8A (PDE8A), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC209169L3 | Lenti ORF clone of Human phosphodiesterase 8A (PDE8A), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC209169L4 | Lenti ORF clone of Human phosphodiesterase 8A (PDE8A), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG209169 | PDE8A (tGFP-tagged) - Human phosphodiesterase 8A (PDE8A), transcript variant 1 |
CNY 7,672.00 |
