MYO1F (NM_012335) Human Untagged Clone
CAT#: SC101088
MYO1F (untagged)-Human myosin IF (MYO1F)
CNY 11,480.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene sequence for NM_012335 edited
GTGAACGGGCAGAAGCAGGGCCATGCCCAAGCCACCCCCAAGATCCCCCTGAACCTGCAC CTCCATCACGACCCATTCAGGAGCCTCCAGGAGCCCAGACACCAGCCCCCCACCATGGGC AGCAAGGAGCGCTTCCACTGGCAGAGCCACAACGTGAAGCAGAGCGGCGTGGATGACATG GTGCTTCTTCCCCAGATCACCGAAGACGCCATTGCCGCCAACCTCCGGAAGCGCTTCATG GACGACTACATCTTCACCTACATCGGCTCTGTGCTCATCTCTGTAAACCCCTTCAAGCAG ATGCCCTACTTCACCGACCGTGAGATCGACCTCTATCAGGGCGCGGCCCAGTATGAGAAT CCCCCGCACATCTACGCCCTCACGGACAACATGTACCGGAACATGCTTATCGACTGTGAG AACCAGTGTGTCATCATTAGTGGAGAGAGTGGAGCTGGGAAGACAGTGGCAGCCAAATAT ATCATGGGCTACATCTCCAAGGTGTCTGGCGGAGGCGAGAAGGTCCAGCACGTCAAAGAT ATCATCCTGCAGTCCAACCCGCTGCTCGAGGCCTTCGGCAACGCCAAGACTGTGCGCAAC AACAATTCCAGCCGCTTTGGCAAGTACTTTGAGATCCAGTTCAGCCGAGGTGGGGAGCCA GATGGGGGCAAGATCTCCAACTTCTTGCTGGAGAAGTCCCGCGTGGTCATGCAAAATGAA AATGAGAGGAACTTCCACATCTACTACCAGCTGCTGGAAGGGGCCTCCCAGGAGCAAAGG CAGAACCTGGGCCTCATGACACCGGACTACTATTACTACCTCAACCAATCGGACACCTAC CAGGTGGACGGCACGGACGACAGAAGCGACTTTGGTGAGACTCTGAGTGCTATGCAGGTT ATTGGGATCCCGCCCAGCATCCAGCAGCTGGTCCTGCAGCTCGTGGCGGGGATCTTGCAC CTGGGGAACATCAGTTTCTGTGAAGACGGGAATTACGCCCGAGTGGAGAGTGTGGACCTC CTGGCCTTTCCCGCCTACCTGCTGGGCATTGACAGCGGGCGACTGCAGGAGAAGCTGACC AGCCGCAAGATGGACAGCCGCTGGGGCGGGCGCAGCGAGTCCATCAATGTGACCCTCAAC GTGGAGCAGGCAGCCTACACCCGTGATGCCCTGGCCAAGGGGCTCTATGCCCGCCTCTTC GACTTCCTCGTGGAGGCCATCAACCGTGCTATGCAGAAACCCCAGGAAGAGTACAGCATC GGTGTGCTGGACATTTACGGCTTCGAGATCTTCCAGAAAAATGGCTTCGAGCAGTTTTGC ATCAACTTCGTCAATGAGAAGCTGCAGCAAATCTTTATCGAACTTACCCTGAAGGCCGAG CAGGAGGAGTATGTGCAGGAAGGCATCCGCTGGACTCCAATCCAGTACTTCAACAACAAG GTCGTCTGTGACCTCATCGAAAACAAGCTGAGCCCCCCAGGCATCATGAGCGTCTTGGAC GACGTGTGCGCCACCATGCACGCCACGGGCGGGGGAGCAGACCAGACACTGCTGCAGAAG CTGCAGGCGGCTGTGGGGACCCACGAGCATTTCAACAGCTGGAGCGCCGGCTTCGTCATC CACCACTACGCTGGCAAGGTCTCCTACGACGTCAGCGGCTTCTGCGAGAGGAACCGAGAC GTTCTCTTCTCCGACCTCATAGAGCTGATGCAGACCAGTGAGCAGGCCTTCCTCCGGATG CTCTTCCCCGAGAAGCTGGATGGAGACAAGAAGGGGCGCCCCAGCACCGCCGGCTCCAAG ATCAAGAAACAAGCCAACGACCTGGTGGCCACACTGATGAGGTGCACACCCCACTACATC CGCTGCATCAAACCCAACGAGACCAAGAGGCCCCGAGACTGGGAGGAGAACAGAGTCAAG CACCAGGTGGAATACCTGGGCCTGAAGGAGAACATCAGGGTGCGCAGAGCCGGCTTCGCC TACCGCCGCCAGTTCGCCAAATTCCTGCAGAGGTATGCCATTCTGACCCCCGAGACGTGG CCGCGGTGGCGTGGGGACGAACGCCAGGGCGTCCAGCACCTGCTTCGGGCGGTCAACATG GAGCCCGACCAGTACCAGATGGGGAGCACCAAGGTCTTTGTCAAGAACCCAGAGTCGCTT TTCCTCCTGGAGGAGGTGCGAGAGCGAAAGTTCGATGGCTTTGCCCGAACCATCCAGAAG GCCTGGCGGCGCCACGTGGCTGTCCGGAAGTACGAGGAGATGCGGGAGGAAGCTTCCAAC ATCCTGCTGAACAAGAAGGAGCGGAGGCGCAACAGCATCAATCGGAACTTCGTCGGGGAC TACCTGGGGCTGGAGGAGCGGCCCGAGCTGCGTCAGTTCCTGGGCAAGAGGGAGCGGGTG GACTTCGCCGATTCGGTCACCAAGTACGACCGCCGCTTCAAGCCCATCAAGCGGGACTTG ATCCTGACGCCCAAGTGTGTGTATGTGATTGGGCGAGAGAAAGTGAAGAAGGGACCTGAG AAGGGCCAGGTGTGTGAAGTCTTGAAGAAGAAAGTGGACATCCAGGCTCTGCGGGGAGTC TCCCTCAGCACGCGACAGGACGACTTCTTCATCCTCCAAGAGGATGCCGCCGACAGCTTC CTGGAGAGCGTCTTCAAGACCGAGTTTGTCAGCCTTCTGTGCAAGCGCTTCGAGGAGGCG ACGCGGAGGCCCCTGCCCCTCACCTTCAGCGACACACTACAGTTTCGGGTGAAGAAGGAG GGCTGGGGCGGTGGCGGCACCCGCAGCGTCACCTTCTCCCGCGGCTTCGGCGACTTGGCA GTGCTCAAGGTTGGCGGTCGGACCCTCACGGTCAGCGTGGGCGATGGGCTGCCCAAGAGC TCCAAGCCTACGCGGAAGGGAATGGCCAAGGGAAAACCTCGGAGGTCGTCCCAAGCCCCT ACCCGGGCGGCCCCTGCGCCCCCCAGAGGCATGGATCGCAATGGGGTGCCCCCCTCTGCC AGAGGGGGCCCCCTGCCCCTGGAGATCATGTCTGGAGGGGGCACCCACAGGCCTCCCCGG GGCCCTCCGTCCACATCCCTGGGAGCCAGCAGACGACCCCGGGCACGTCCGCCCTCAGAG CACAACACAGAATTCCTCAACGTGCCTGACCAGGGCATGGCCGGCATGCAGAGGAAGCGC AGCGTGGGGCAACGGCCAGTGCCTGGTGTGGGCCGACCCAAGCCCCAGCCTCGGACACAT GGTCCCAGGTGCCGGGCCCTATACCAGTACGTGGGCCAAGATGTGGACGAGCTGAGCTTC AACGTGAACGAGGTCATTGAGATCCTCATGGAAGATCCCTCGGGCTGGTGGAAGGGCCGG CTTCACGGCCAGGAGGGCCTTTTCCCAGGAAACTACGTGGAGAAGATCTGAGCTGGGCCC TGGGATACTGCCTTCTCTTTCGCCCGCCTATCTGCCTGCCGGCCTGGTGGGGAGCCAGGC CCTGCCAATGAGAGCCTCGTTTACCTGGGCTGCAATAGCCTAAAAGTCCAGTCCTTTGGC CTCCAGTCCTGCCCAGGCCCTGGGTCACCAGGTCACTGCTGCAGCCCCCGCCCCTGGGCC CTGGTCTTCCTCCAACATCACACCTGCTGCCCATTCTCCATTTCTGTGTGTGTCAAAGGG GACTAACAGCAGAATCTACCTCCCAACTGCCATGTGATTAAGAAATGGGTCTTGAGTCCT GTGCTGTTGGCAAAGTGCCAGGCACAGTTGGGGAGGGGGGGGTCCTTAACAAGCGTGACT TTGCTCATTCTGTCATCACTAAGGCAATAAACCTTTGCCAGGTGAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAA |
Restriction Sites | NotI-NotI |
ACCN | NM_012335 |
Insert Size | 3800 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | The ORF of this clone has been fully sequenced and found to be a perfectmatch to NM_012335.2. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_012335.2, NP_036467.2 |
RefSeq Size | 3860 bp |
RefSeq ORF | 3297 bp |
Locus ID | 4542 |
UniProt ID | O00160 |
Domains | IQ, SH3, myosin_head |
Gene Summary | Myosins are molecular motors that use the energy from ATP hydrolysis to generate force on actin filaments. The protein encoded by this gene is an unconventional myosin that may be involved in the intracellular movement of membrane-enclosed compartments. There is evidence to suggest that mutations in this gene can result in hearing loss. [provided by RefSeq, Jan 2017] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC207069 | MYO1F (Myc-DDK-tagged)-Human myosin IF (MYO1F) |
CNY 11,464.00 |
|
RC207069L1 | Lenti ORF clone of Human myosin IF (MYO1F), Myc-DDK-tagged |
CNY 13,864.00 |
|
RC207069L2 | Lenti ORF clone of Human myosin IF (MYO1F), mGFP tagged |
CNY 13,864.00 |
|
RC207069L3 | Lenti ORF clone of Human myosin IF (MYO1F), Myc-DDK-tagged |
CNY 10,260.00 |
|
RC207069L4 | Lenti ORF clone of Human myosin IF (MYO1F), mGFP tagged |
CNY 10,260.00 |
|
RG207069 | MYO1F (tGFP-tagged) - Human myosin IF (MYO1F) |
CNY 13,064.00 |