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TTL (NM_153712) Human Untagged Clone
CAT＃: SC100834
TTL (untagged)-Human tubulin tyrosine ligase (TTL)
CNY 17,416.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites | NotI-NotI |
| ACCN | NM_153712 |
| Insert Size | 4700 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_153712.3, NP_714923.1 |
| RefSeq Size | 5181 bp |
| RefSeq ORF | 5181 bp |
| Locus ID | 150465 |
| UniProt ID | Q8NG68 |
| Domains | TTL |
| Gene Summary | TTL is a cytosolic enzyme involved in the posttranslational modification of alpha-tubulin (see MIM 602529). Alpha-tubulin within assembled microtubules is detyrosinated over time at the C terminus. After microtubule disassembly, TTL restores the tyrosine residues and consequently participates in a cycle of tubulin detyrosination and tyrosination (Erck et al., 2003 [PubMed 14571137]).[supplied by OMIM, Mar 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
AMPK activation promotes lipid droplet dispersion on detyrosinated microtubules to increase mitochondrial fatty acid oxidation
,null,
Nature Communications
,PubMed ID 26013497
[TTL]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207805 | TTL (Myc-DDK-tagged)-Human tubulin tyrosine ligase (TTL) |
CNY 5,488.00 |
|
| RC207805L1 | Lenti ORF clone of Human tubulin tyrosine ligase (TTL), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC207805L2 | Lenti ORF clone of Human tubulin tyrosine ligase (TTL), mGFP tagged |
CNY 7,888.00 |
|
| RC207805L3 | Lenti ORF clone of Human tubulin tyrosine ligase (TTL), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207805L4 | Lenti ORF clone of Human tubulin tyrosine ligase (TTL), mGFP tagged |
CNY 5,890.00 |
|
| RG207805 | TTL (tGFP-tagged) - Human tubulin tyrosine ligase (TTL) |
CNY 7,088.00 |
