Phospholipase A2 (PLA2G4A) (NM_024420) Human Untagged Clone
CAT#: SC100383
PLA2G4A (untagged)-Human phospholipase A2, group IVA (cytosolic, calcium-dependent) (PLA2G4A)
CNY 5,496.00
CNY 7,220.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | cPLA2; cPLA2-alpha; GURDP; PLA2G4 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC100383 sequence for NM_024420 edited (data generated by NextGen Sequencing)
ATGTCATTTATAGATCCTTACCAGCACATTATAGTGGAGCACCAGTATTCCCACAAGTTT ACGGTAGTGGTGTTACGTGCCACCAAAGTGACAAAGGGGGCCTTTGGTGACATGCTTGAT ACTCCAGATCCCTATGTGGAACTTTTTATCTCTACAACCCCTGACAGCAGGAAGAGAACA AGACATTTCAATAATGACATAAACCCTGTGTGGAATGAGACCTTTGAATTTATTTTGGAT CCTAATCAGGAAAATGTTTTGGAGATTACGTTAATGGATGCCAATTATGTCATGGATGAA ACTCTAGGGACAGCAACATTTACTGTATCTTCTATGAAGGTGGGAGAAAAGAAAGAAGTT CCTTTTATTTTCAACCAAGTCACTGAAATGGTTCTAGAAATGTCTCTTGAAGTTTGCTCA TGCCCAGACCTACGATTTAGTATGGCTCTGTGTGATCAGGAGAAGACTTTCAGACAACAG AGAAAAGAACACATAAGGGAGAGCATGAAGAAACTCTTGGGTCCAAAGAATAGTGAAGGA TTGCATTCTGCACGTGATGTGCCTGTGGTAGCCATATTGGGTTCAGGTGGGGGTTTCCGA GCCATGGTGGGATTCTCTGGTGTGATGAAGGCATTATACGAATCAGGAATTCTGGATTGT GCTACCTACGTTGCTGGTCTTTCTGGCTCCACCTGGTATATGTCAACCTTGTATTCTCAC CCTGATTTTCCAGAGAAAGGGCCAGAGGAGATTAATGAAGAACTAATGAAAAATGTTAGC CACAATCCCCTTTTACTTCTCACACCACAGAAAGTTAAAAGATATGTTGAGTCTTTATGG AAGAAGAAAAGCTCTGGACAACCTGTCACCTTTACTGATATCTTTGGGATGTTAATAGGA GAAACACTAATTCATAATAGAATGAATACTACTCTGAGCAGTTTGAAGGAAAAAGTTAAT ACTGCACAATGCCCTTTACCTCTTTTCACCTGTCTTCATGTCAAACCTGACGTTTCAGAG CTGATGTTTGCAGATTGGGTTGAATTTAGTCCATACGAAATTGGCATGGCTAAATATGGT ACTTTTATGGCTCCCGACTTATTTGGAAGCAAATTTTTTATGGGAACAGTCGTTAAGAAG TATGAAGAAAACCCCTTGCATTTCTTAATGGGTGTCTGGGGCAGTGCCTTTTCCATATTG TTCAACAGAGTTTTGGGCGTTTCTGGTTCACAAAGCAGAGGCTCCACAATGGAGGAAGAA TTAGAAAATATTACCACAAAGCATATTGTGAGTAATGATAGCTCGGACAGTGATGATGAA TCACACGAACCCAAAGGCACTGAAAATGAAGATGCTGGAAGTGACTATCAAAGTGATAAT CAAGCAAGTTGGATTCATCGTATGATAATGGCCTTGGTGAGTGATTCAGCTTTATTCAAT ACCAGAGAAGGACGTGCTGGGAAGGTACACAACTTCATGCTGGGCTTGAATCTCAATACA TCTTATCCACTGTCTCCTTTGAGTGACTTTGCCACACAGGACTCCTTTGATGATGATGAA CTGGATGCAGCTGTAGCAGATCCTGATGAATTTGAGCGAATATATGAGCCTCTGGATGTC AAAAGTAAAAAGATTCATGTAGTGGACAGTGGGCTCACATTTAACCTGCCGTATCCCTTG ATACTGAGACCTCAGAGAGGGGTTGATCTCATAATCTCCTTTGACTTTTCTGCAAGGCCA AGTGACTCTAGTCCTCCGTTCAAGGAACTTCTACTTGCAGAAAAGTGGGCTAAAATGAAC AAGCTCCCCTTTCCAAAGATTGATCCTTATGTGTTTGATCGGGAAGGGCTGAAGGAGTGC TATGTCTTTAAACCCAAGAATCCTGATATGGAGAAAGATTGCCCAACCATCATCCACTTT GTTCTGGCCAACATCAACTTCAGAAAGTACAAGGCTCCAGGTGTTCCAAGGGAAACTGAG GAAGAGAAAGAAATCGCTGACTTTGATATTTTTGATGACCCAGAATCACCATTTTCAACC TTCAATTTTCAATATCCAAATCAAGCATTCAAAAGACTACATGATCTTATGCACTTCAAT ACTCTGAACAACATTGATGTGATAAAAGAAGCCATGGTTGAAAGCATTGAATATAGAAGA CAGAATCCATCTCGTTGCTCTGTTTCCCTTAGTAATGTTGAGGCAAGAAGATTTTTCAAC AAGGAGTTTCTAAGTAAACCCAAAGCATAG Clone variation with respect to NM_024420.2 >OriGene 5' read for NM_024420 unedited
GGGTTCAGAATTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGAGATTCTC AGGTTTTAAAGACGCTAGAGTGCCAAAGAAGACTTTGAAGTGTGAAAACATTTCCTGTAA TTGAAACCAAAATGTCATTTATAGATCCTTACCAGCACATTATAGTGGAGCACCAGTATT CCCACAAGTTTACGGTAGTGGTGTTACGTGCCACCAAAGTGACAAAGGGGGCCTTTGGTG ACATGCTTGATACTCCAGATCCCTATGTGGAACTTTTTATCTCTACAACCCCTGACAGCA GGAAGAGAACAAGACATTTCAATAATGACATAAACCCTGTGTGGAATGAGACCTTTGAAT TTATTTTGGATCCTAATCAGGAAAATGTTTTGGAGATTACGTTAATGGATGCCAATTATG TCATGGATGAAACTCTAGGGACAGCAACATTTACTGTATCTTCTATGAAGGTGGGAGAAA AGAAAGAAGTTCCTTTTATTTTCAACCAAGTCACTGAAATGGTTCTAGAAATGTCTCTTG AAGTTTGCTCATGCCCAGACCTACGATTTAGTATGGCTCTGTGTGATCAGGAGAAGACTT TCAGACAACAGAGAAAAGAACACATAAGGGAGAGCATGAAGAAACTCTTGGGTCCAAAGA ATAGTGAAGGATTGCATTCTGCACGTGATGTGCCTGTGGTAGCCATATTGGGTTCAGGTG GNGGTTTCCGAGCCATGGTGGGATTCTCTGGTGTGATGAAAGCATTATACGAATCANGAA TTCTGGATTGTGCTACCTACGTTGCTGGTCTTTCTGGCTCCACTGTTATATGTCAACCTT GTATCCTCACCCTGATTTCCAGAGAAGGGCCCNGAGAGATTATGAAGAACTATGAAAATG TTGCCCAATCCCCTTTACT >OriGene 3' read for NM_024420 unedited
GTCCGCGGCCGCATCTATAGTGAGTTTTTTTTTTTTTTTTTTTTATTTTTATATAGATGT ATTTATTTCATGTATGTATATATATGCCTATATAAATAAAAATAGTGTTGTCTCATGGTA TGAATAAATCTAGTCAGTGAATAACATTCATACCTAATGCGAGGTCTTATTGAGATTGAA CTGTTAAATATTTTAAAAGAAAAAAAATTTTTAAAAAGAACACACCTAAGAAAGTTGGTG AGAAATGTTTAAAAATACAGTATATATCCCTACATTTGTATCAAGAAGTTCATACTGACT GAAAATGTAGCTAAGTATATCCTTACATAATCAACATCATTTGTCAACCTAACTTATAAT AGTATTATTCTCATGCAGCTAAGTAACTGCAACTTTGAGTATCAGCCAGTCTCTCATGAT CAGTACGACTATCTGTACTGTACTTTTAAATCCAGTTGTCATGGGATTGCAAACTGCCTC AGCATCAGAAACTGCTGCCATTTCCAGTACATGAACTATGCTTTGGGTTTACTTAGAAAC TCCTTGTTGAAAAATCTTCTTGCCTCAACATTACTAAGGGAAACAGAGCAACGAGATGGA TTCTGTCTTCTATATTCAATGCTTTCAACCATGGCTTCTTTTATCACATCAATGTTGTTC AGAGTATTGAAGCGCATAAGATCATGTAGTCTTTTGAATGCCTGATTTGGATATTGAAAA TTGAAAGGTGAAAATGGCGATTCTGGGTCATCAAAAATATCANAGTCAGCGATTTCTTTC TCTTCCTCAGTTTCCCTAGAACACCTGNAGCCNTGTACTTTCTGAGNTGATGNTGCCANA ACAAGTGGATGATGGNTAGGGCATCTTCTCCTATTAGATTCTAGGTTTAAGACTANACTC CTTCACCCTCCGATCAAACCNTAGATCATCTTTGAAGGGGGAGCTGTCATTTACCCACTT NCTGCAGTAAATTCCTTGACGAGNCTANATNCCTNGNCCTGCNAAATCAAGAGATTGAAA TCACCCNTCTGG |
Restriction Sites | NotI-NotI |
ACCN | NM_024420 |
Insert Size | 2900 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_024420.1, NP_077734.1 |
RefSeq Size | 2875 bp |
RefSeq ORF | 2250 bp |
Locus ID | 5321 |
UniProt ID | P47712 |
Domains | C2, PLA2_B |
Protein Pathways | alpha-Linolenic acid metabolism, Arachidonic acid metabolism, Ether lipid metabolism, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Glycerophospholipid metabolism, GnRH signaling pathway, Linoleic acid metabolism, Long-term depression, MAPK signaling pathway, Metabolic pathways, Vascular smooth muscle contraction, VEGF signaling pathway |
Gene Summary | This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC220972 | PLA2G4A (Myc-DDK-tagged)-Human phospholipase A2, group IVA (cytosolic, calcium-dependent) (PLA2G4A) |
CNY 8,232.00 |
|
RC220972L1 | Lenti ORF clone of Human phospholipase A2, group IVA (cytosolic, calcium-dependent) (PLA2G4A), Myc-DDK-tagged |
CNY 10,632.00 |
|
RC220972L2 | Lenti ORF clone of Human phospholipase A2, group IVA (cytosolic, calcium-dependent) (PLA2G4A), mGFP tagged |
CNY 10,632.00 |
|
RC220972L3 | Lenti ORF clone of Human phospholipase A2, group IVA (cytosolic, calcium-dependent) (PLA2G4A), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC220972L4 | Lenti ORF clone of Human phospholipase A2, group IVA (cytosolic, calcium-dependent) (PLA2G4A), mGFP tagged |
CNY 5,890.00 |
|
RG220972 | PLA2G4A (tGFP-tagged) - Human phospholipase A2, group IVA (cytosolic, calcium-dependent) (PLA2G4A) |
CNY 9,832.00 |
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