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UBA1 (NM_153280) Human Untagged Clone
CAT＃: SC100353
UBA1 (untagged)-Human ubiquitin-like modifier activating enzyme 1 (UBA1), transcript variant 2
CNY 7,376.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1; POC20; SMAX2; UBA1A; UBE1; UBE1X; VEXAS |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_153280, the custom clone sequence may differ by one or more nucleotides
ATGTCCAGCTCGCCGCTGTCCAAGAAACGTCGCGTGTCCGGGCCTGATCCAAAGCCGGGTTCTAACTGCT CCCCTGCCCAGTCCGTGTTGTCCGAAGTGCCCTCGGTGCCAACCAACGGAATGGCCAAGAACGGCAGTGA AGCAGACATAGACGAGGGCCTTTACTCCCGGCAGCTGTATGTGTTGGGCCATGAGGCAATGAAGCGGCTC CAGACATCCAGTGTCCTGGTATCAGGCCTGCGGGGCCTGGGCGTGGAGATCGCTAAGAACATCATCCTTG GTGGGGTCAAGGCTGTTACCCTACATGACCAGGGCACTGCCCAGTGGGCTGATCTTTCCTCCCAGTTCTA CCTGCGGGAGGAGGACATCGGTAAAAACCGGGCCGAGGTATCACAGCCCCGCCTCGCTGAGCTCAACAGC TATGTGCCTGTCACTGCCTACACTGGACCCCTCGTTGAGGACTTCCTTAGTGGTTTCCAGGTGGTGGTGC TCACCAACACCCCCCTGGAGGACCAGCTGCGAGTGGGTGAGTTCTGTCACAACCGTGGCATCAAGCTGGT GGTGGCAGACACGCGGGGCCTGTTTGGGCAGCTCTTCTGTGACTTTGGAGAGGAAATGATCCTCACAGAT TCCAATGGGGAGCAGCCACTCAGTGCTATGGTTTCTATGGTTACCAAGGACAACCCCGGTGTGGTTACCT GCCTGGATGAGGCCCGACACGGGTTTGAGAGCGGGGACTTTGTCTCCTTTTCAGAAGTACAGGGCATGGT TGAACTCAACGGAAATCAGCCCATGGAGATCAAAGTCCTGGGTCCTTATACCTTTAGCATCTGTGACACC TCCAACTTCTCCGACTACATCCGTGGAGGCATCGTCAGTCAGGTCAAAGTACCTAAGAAGATTAGCTTTA AATCCTTGGTGGCCTCACTGGCAGAACCTGACTTTGTGGTGACGGACTTCGCCAAGTTTTCTCGCCCTGC CCAGCTGCACATTGGCTTCCAGGCCCTGCACCAGTTCTGTGCTCAGCATGGCCGGCCACCTCGGCCCCGC AATGAGGAGGATGCAGCAGAACTGGTAGCCTTAGCACAGGCTGTGAATGCTCGAGCCCTGCCAGCAGTGC AGCAAAATAACCTGGACGAGGACCTCATCCGGAAGCTGGCATATGTGGCTGCTGGGGATCTGGCACCCAT AAACGCCTTCATTGGGGGCCTGGCTGCCCAGGAAGTCATGAAGGCCTGCTCCGGGAAGTTCATGCCCATC ATGCAGTGGCTATACTTTGATGCCCTTGAGTGTCTCCCTGAGGACAAAGAGGTCCTCACAGAGGACAAGT GCCTCCAGCGCCAGAACCGTTATGACGGGCAAGTGGCTGTGTTTGGCTCAGACCTGCAAGAGAAGCTGGG CAAGCAGAAGTATTTCCTGGTGGGTGCGGGGGCCATTGGCTGTGAGCTGCTCAAGAACTTTGCCATGATT GGGCTGGGCTGCGGGGAGGGTGGAGAAATCATCGTTACAGACATGGACACCATTGAGAAGTCAAATCTGA ATCGACAGTTTCTTTTCCGGCCCTGGGATGTCACGAAGTTAAAGTCTGACACGGCTGCTGCAGCTGTGCG CCAAATGAATCCACATATCCGGGTGACAAGCCACCAGAACCGTGTGGGTCCTGACACGGAGCGCATCTAT GATGACGATTTTTTCCAAAACCTAGATGGCGTGGCCAATGCCCTGGACAACGTGGATGCCCGCATGTACA TGGACCGCCGCTGTGTCTACTACCGGAAGCCACTGCTGGAGTCAGGCACACTGGGCACCAAAGGCAATGT GCAGGTGGTGATCCCCTTCCTGACAGAGTCGTACAGTTCCAGCCAGGACCCACCTGAGAAGTCCATCCCC ATCTGTACCCTGAAGAACTTCCCTAATGCCATCGAGCACACCCTGCAGTGGGCTCGGGATGAGTTTGAAG GCCTCTTCAAGCAGCCAGCAGAAAATGTCAACCAGTACCTCACAGACCCCAAGTTTGTGGAGCGAACACT GCGGCTGGCAGGCACTCAGCCCTTGGAGGTGCTGGAGGCTGTGCAGCGCAGCCTGGTGCTGCAGCGACCA CAGACCTGGGCTGACTGCGTGACCTGGGCCTGCCACCACTGGCACACCCAGTACTCGAACAACATCCGGC AGCTGCTGCACAACTTCCCTCCTGACCAGCTCACAAGCTCAGGAGCGCCGTTCTGGTCTGGGCCCAAACG CTGTCCACACCCGCTCACCTTTGATGTCAACAATCCCCTGCATCTGGACTATGTGATGGCTGCTGCCAAC CTGTTTGCCCAGACCTACGGGCTGACAGGCTCTCAGGACCGAGCTGCTGTGGCCACATTCCTGCAGTCTG TGCAGGTCCCCGAATTCACCCCCAAGTCTGGCGTCAAGATCCATGTTTCTGACCAGGAGCTGCAGAGCGC CAATGCCTCTGTTGATGACAGTCGTCTAGAGGAGCTCAAAGCCACTCTGCCCAGCCCAGACAAGCTCCCT GGATTCAAGATGTACCCCATTGACTTTGAGAAGGATGATGACAGCAACTTTCATATGGATTTCATCGTGG CTGCATCCAACCTCCGGGCAGAAAACTATGACATTCCTTCTGCAGACCGGCACAAGAGCAAGCTGATTGC AGGGAAGATCATCCCAGCCATTGCCACGACCACAGCAGCCGTGGTTGGCCTTGTGTGTCTGGAGCTGTAC AAGGTTGTGCAGGGGCACCGACAGCTTGACTCCTACAAGAATGGTTTCCTCAACTTGGCCCTGCCTTTCT TTGGTTTCTCTGAACCCCTTGCCGCACCACGTCACCAGTACTATAACCAAGAGTGGACATTGTGGGATCG CTTTGAGGTACAAGGGCTGCAGCCTAATGGTGAGGAGATGACCCTCAAACAGTTCCTCGACTATTTTAAG ACAGAGCACAAATTAGAGATCACCATGCTGTCCCAGGGCGTGTCCATGCTCTATTCCTTCTTCATGCCAG CTGCCAAGCTCAAGGAACGGTTGGATCAGCCGATGACAGAGATTGTGAGCCGTGTGTCGAAGCGAAAGCT GGGCCGCCACGTGCGGGCGCTGGTGCTTGAGCTGTGCTGTAACGACGAGAGCGGCGAGGATGTCGAGGTT CCCTATGTCCGATACACCATCCGCTGA >OriGene 5' read for NM_153280 unedited
GGTGGCACCATTTTGTAATACGACTCACTATTAGGGCGGCCGCGATTCGCCACGAGGCGG CATTGGTTCTATGCTCTGTGTTATTTATCATCTCGATAACCCCGTGGGGAAGATGTCCAG CTCGCCGCTGTCCAAGAAACGTCGCGTGTCCGGGCCTGATCCAAAGCCGGGTTCTAACTG CTCCCCTGCCCAGTCCGTGTTGTCCGAAGTGCCCTCGGTGCCAACCAACGGAATGGCCAA GAACGGCAGTGAAGCAGACATAGACGAGGGCCTTTACTCCCGGCAGCTGTATGTGTTGGG CCATGAGGCAATGAAGCGGCTCCAGACATCCAGTGTCCTGGTATCAGGCCTGCGGGGCCT GGGCGTGGAGATCGCTAAGAACATCATCCTTGGTGGGGTCAAGGCTGTTACCCTACATGA CCAGGGCACTGCCCAGTGGGCTGATCTTTCCTCCCAGTTCTACCTGCGGGAGGAGGACAT CGGTAAAAACCGGGCCGAGGTATCACAGCCCCGCCTCGCTGAGCTCAACAGCTATGTGCC TGTCACTGCCTACACTGGACCCCTCGTTGAGGACTTCCTTAGTGGTTTCCAGGTGGTGGT GCTCACCAACACCCCCCTGGAGGACCAGCTGCGAGTGGGTGAGTTCTGTCACAACCGTGG CATCAAGCTGGTGGTGGCAGACACGCGGGGCCTGTTTGGGCAGCTCTTCTGTGACTTTGG AGAGGAAATGATCCTCACAGATTCCAATGGGGAGCAGCCACTCAGTGCTATGGTTCTATG GTTACACAGACACCCCCGTGTTGGTACCCTGCCTGATGANGCCCGACACGGGTTTGAGAG CGGGGACTTTGTCTTCTTTTANAAGTCAGGGCATGTTTGTACTCAACGGAATTAGCCCAT GAGGAACAAGTCTGGGNCCTATACCTTAACAT >OriGene 3' read for NM_153280 unedited
AACGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTGGAGTTATTAATTCTT TATTAGGATTCAGGTTTCAAACAAGGTAGAAGGCAGTGGCAAGAGGGGTTCAGGTAGGGG GATGATGAGGGAACACCAGACTTGGCTAGTTGGGCCACTGCCAGAAGCCAAATGGGAACC CTGGGCTGGAAGGGGTGTGGAGAGGGGTGGACAAGGGGCCAGCCTAGAGGAGCAGACGGG GTCAGCGGATGGTGTATCGGACATAGGGAACCTCGACATCCTCGCCGCTCTCGTCGTTAC AGCACAGCTCAAGCACCAGCGCCCGCACGTGGCGGCCCAGCTTTCGCTTCGACACACGGC TCACAATCTCTGTCATCGGCTGATCCAACCGTTCCTTGAGCTTGGCAGCTGGCATGAAGA AGGAATAGAGCATGGACACGCCCTGGGACAGCATGGTGATCTCTAATTTGTGCTCTGTCT TAAAATAGTCGAGGAACTGTTTGAGGGTCATCTCCTCACCATTAGGCTGCAGCCCTTGTA CCTCAAAGCGATCCCACAATGTCCACTCTTGGTTATAGTACTGGTGACGTGGTGCGGCAA GGGGTTCAGAGAAACCAAAGAAAGGCAGGGCCAAGTTGAGGAAACCATTCTTGTANGAGT CAAGCTGTCGGTGCCCCTGCACAACCTTGTACAGCTNCAGACACACAAGGCCAACCACGG CTGCTGTGGTCGTGGGCATGGCTGGGATGATCTTCCCTGCAATCAGCTTGCTCTTGTGCC NGTCTGCANAAGGAATGTCATANTTTTCTGCCCCGAGGTTGGATGCAGCCCCGATGAAAT CCTATGAAAAGTGCTGGCATCATCCTTCTCAAGTCAATGGGTACATCCTGAATCCAGGNA GCTTGCTTGGGCTGGCAAAATGGCCTTGAACTCTCTTAACAATTGCATTAACAGAGCTTT GGGCTTGCAGCTCCTTTCAAAAATGGGTCTTGTAGCCGACTGGGGGTGAATCGGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_153280 |
| Insert Size | 3500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_153280.1, NP_695012.1 |
| RefSeq Size | 3483 bp |
| RefSeq ORF | 3177 bp |
| Locus ID | 7317 |
| UniProt ID | P22314 |
| Domains | UBACT, ThiF |
| Protein Pathways | Parkinson's disease, Ubiquitin mediated proteolysis |
| Gene Summary | The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
UBA1/GARS-dependent pathways drive sensory-motor connectivity defects in spinal muscular atrophy
,Shorrock, HK;van der Hoorn, D;Boyd, PJ;Llavero Hurtado, M;Lamont, DJ;Wirth, B;Sleigh, JN;Schiavo, G;Wishart, TM;Groen, EJN;Gillingwater, TH;,
Brain
,PubMed ID 30239612
[UBA1]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219513 | UBA1 (Myc-DDK-tagged)-Human ubiquitin-like modifier activating enzyme 1 (UBA1), transcript variant 2 |
CNY 7,368.00 |
|
| RC219513L1 | Lenti ORF clone of Human ubiquitin-like modifier activating enzyme 1 (UBA1), transcript variant 2, Myc-DDK-tagged |
CNY 9,768.00 |
|
| RC219513L2 | Lenti ORF clone of Human ubiquitin-like modifier activating enzyme 1 (UBA1), transcript variant 2, mGFP tagged |
CNY 7,600.00 |
|
| RC219513L3 | Lenti ORF clone of Human ubiquitin-like modifier activating enzyme 1 (UBA1), transcript variant 2, Myc-DDK-tagged |
CNY 7,600.00 |
|
| RC219513L4 | Lenti ORF clone of Human ubiquitin-like modifier activating enzyme 1 (UBA1), transcript variant 2, mGFP tagged |
CNY 7,600.00 |
|
| RG219513 | UBA1 (tGFP-tagged) - Human ubiquitin-like modifier activating enzyme 1 (UBA1), transcript variant 2 |
CNY 8,968.00 |
