HIBADH (NM_152740) Human Untagged Clone
CAT#: SC100318
HIBADH (untagged)-Human 3-hydroxyisobutyrate dehydrogenase (HIBADH)
CNY 3,656.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | NS5ATP1 |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC100318 sequence for NM_152740 edited (data generated by NextGen Sequencing)
ATGGCAGCCTCCTTACGGCTCCTCGGAGCTGCCTCCGGTCTCCGGTACTGGAGCCGGCGG CTGCGGCCGGCAGCCGGCAGCTTTGCAGCGGTGTGTTCTAGGTCAGTGGCTTCAAAGACT CCAGTTGGATTCATTGGACTGGGCAACATGGGGAATCCAATGGCAAAAAATCTCATGAAA CATGGCTATCCACTTATTATTTATGATGTGTTCCCTGATGCCTGCAAAGAGTTTCAAGAT GCAGGTGAACAGGTAGTATCTTCCCCAGCAGATGTTGCTGAAAAAGCTGACAGAATTATT ACAATGCTGCCCACCAGTATCAATGCAATAGAAGCTTATTCCGGAGCAAATGGGATTCTA AAAAAAGTGAAGAAGGGCTCATTATTAATAGATTCCAGCACTATTGATCCTGCAGTTTCA AAAGAATTGGCCAAAGAAGTTGAGAAAATGGGAGCAGTTTTCATGGATGCCCCTGTTTCT GGTGGTGTAGGAGCTGCACGATCTGGGAACCTCACGTTTATGGTGGGAGGAGTTGAAGAT GAATTTGCTGCTGCCCAAGAGTTGCTGGGGTGCATGGGCTCCAACGTGGTGTACTGTGGA GCTGTTGGGACTGGGCAGGCGGCAAAGATCTGCAACAACATGCTGTTAGCTATTAGTATG ATTGGAACTGCTGAAGCTATGAATCTTGGAATCAGGTTAGGGCTTGACCCAAAACTACTG GCTAAAATCCTAAATATGAGCTCAGGACGGTGTTGGTCAAGTGACACTTATAATCCTGTA CCTGGAGTGATGGATGGCGTTCCCTCGGCTAATAACTATCAGGGTGGATTTGGAACAACA CTCATGGCTAAGGATCTGGGATTGGCACAAGACTCTGCTACCAGCACAAAGAGCCCAATC CTTCTTGGCAGTCTGGCCCATCAGATCTACAGGATGATGTGTGCAAAGGGCTACTCAAAG AAAGACTTCTCATCCGTGTTCCAGTTCCTACGAGAGGAGGAGACCTTCTGA Clone variation with respect to NM_152740.3 >OriGene 5' read for NM_152740 unedited
ACAATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCGCGCGCCCTTC TCCGTGGGCGAGCCAGCCAGTCCCGCTGCACACGCTCGCAGTCTGTGGGCCCTCCGGGAG GCGGCGGAGGTCACCGCGGGGAGAGGGGCGGGCGCAGCATGGCAGCCTCCTTACGGCTCC TCGGAGCTGCCTCCGGTCTCCGGTACTGGAGCCGGCGGCTGCGGCCGGCAGCCGGCAGCT TTGCAGCGGTGTGTTCTAGGTCAGTGGCTTCAAAGACTCCAGTTGGATTCATTGGACTGG GCAACATGGGGAATCCAATGGCAAAAAATCTCATGAAACATGGCTATCCACTTATTATTT ATGATGTGTTCCCTGATGCCTGCAAAGAGTTTCAAGATGCAGGTGAACAGGTAGTATCTT CCCCAGCAGATGTTGCTGAAAAAGCTGACAGAATTATTACAATGCTGCCCACCAGTATCA ATGCAATAGAAGCTTATTCCGGAGCAAATGGGATTCTAAAAAAAGTGAAGAAGGGCTCAT TATTAATAGATTCCAGCACTATTGATCCTGCAGTTTCAAAAGAATTGGCCAAAGAAGTTG AGAAAATGGGAGCAGTTTTCATGGATGCCCCTGTTTCTGGTGGTGTAGGAGCTGCACGAT CTGGGAACCTCACGTTTATGGTGGGAGGAGTTGAAGATGAATTTGCTGCTGCCCAAGAGT TGCTGGGGTGCATGGGCTCCAACGTGGTGTACTGTGGAGCTGTTGGGACTGGGCANGCGG CANAGATCTGCAACACATGCTGNTAGCTATTAGTATGATTGGAACTGCTGAAGCTATGAT CTGGAATCANGTTAGGCTGACCCAAACTACTGGCTAAATCCTAATATGAGCTCNNGACGT GTGGTCAGTGACACTATATNCTGTACTGNATGATGGATGGCGTCCTCGCTATACTATAGG TGGGATTGACACCTCAGNCTAGACTGGATN >OriGene 3' read for NM_152740 unedited
NAATTTAGCTATGNACCGCGGCCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTTT TTTTTACCAGTATAGAAATTTATTTGAATTCAAAATAATATGGCTATATTTATAATAATA TAATAATTATCTAAAGCAAATATCATCATTGGCTCTGAAATGCGTCTAAAGATGTCATTC TTAAGTCAAAAAATACGTAGTAAGAATGTACAAGAAAGAAAAAAATATAAAAACAAGTCT GCTGAGTGTCGGGAGTTGGTGAGGGATATCCTACCATATTGTGACGGAGTCCAAATAGAA AACATGCAGCAACAGTTCTCCTGCTTTATCAGCTCCCTGGAAAATAAACCAGTAACCCTG GTAGTGCAGTAACCATTTGGTTAACAGGACAAACTTCCTGATGGACACAGATAGTAATTC ACTGCATTTCCCTTCTCTAACTTCTCTCTTCACACCAATTCCTTTTCTTTCCTTTAAGAT GGGTTTCATCCTGTTGACAAAAGATTTGGTTTTATTTGTAAAGTAAAGCAGATAATATCC TGATTGAAGTATTCAATGATTTAGTTGAGGATGCTTGGGGATCAAACTTTGTAAAAAGGT CAATTAAGCTAGTTAGCAGAGACTATCAGTGGCTTGCAGAAAAAAAATTCGGATAATATG TTTGTTAAAAAGACAAAAAGAATAAGCGGTGAAAAATCCTAGGGATTACTGTGACCTAGA CAATCAAAAGCAGATAGGTGACCTTTGATTAAATCCATTTNACTTGTGGAGTGAGCTAAA AGGAGGCTCCAAGACAGAGTTTGGTTCCCAACAGTGTCCGTGGCCAAAGGGCACACTCAG AAAGGTCTCTCCTCTNCGTAGACTGGNACACGATGAGAAGTCTTTCCTTGAGTAGCCTTT GCACACTCATCCTGTAGATCTGATGGGCCNAC |
Restriction Sites | NotI-NotI |
ACCN | NM_152740 |
Insert Size | 2000 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_152740.2, NP_689953.1 |
RefSeq Size | 2006 bp |
RefSeq ORF | 1011 bp |
Locus ID | 11112 |
UniProt ID | P31937 |
Domains | NAD_binding_2 |
Protein Pathways | Metabolic pathways, Valine, leucine and isoleucine degradation |
Gene Summary | This gene encodes a mitochondrial 3-hydroxyisobutyrate dehydrogenase enzyme. The encoded protein plays a critical role in the catabolism of L-valine by catalyzing the oxidation of 3-hydroxyisobutyrate to methylmalonate semialdehyde. [provided by RefSeq, Nov 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC207125 | HIBADH (Myc-DDK-tagged)-Human 3-hydroxyisobutyrate dehydrogenase (HIBADH) |
CNY 5,488.00 |
|
RC207125L1 | Lenti ORF clone of Human 3-hydroxyisobutyrate dehydrogenase (HIBADH), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC207125L2 | Lenti ORF clone of Human 3-hydroxyisobutyrate dehydrogenase (HIBADH), mGFP tagged |
CNY 7,888.00 |
|
RC207125L3 | Lenti ORF clone of Human 3-hydroxyisobutyrate dehydrogenase (HIBADH), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC207125L4 | Lenti ORF clone of Human 3-hydroxyisobutyrate dehydrogenase (HIBADH), mGFP tagged |
CNY 5,890.00 |
|
RG207125 | HIBADH (tGFP-tagged) - Human 3-hydroxyisobutyrate dehydrogenase (HIBADH) |
CNY 7,088.00 |