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Lass5 (CERS5) (NM_147190) Human Untagged Clone
CAT＃: SC100282
CERS5 (untagged)-Human LAG1 homolog, ceramide synthase 5 (LASS5)
CNY 3,656.00
| Cited in 3 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | LASS5; Trh4 |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC100282 sequence for NM_147190 edited (data generated by NextGen Sequencing)
ATGGCGACAGCAGCGCAGGGACCCCTAAGCTTGCTGTGGGGCTGGCTGTGGAGCGAGCGC TTCTGGCTACCCGAGAACGTGAGCTGGGCTGATCTGGAGGGGCCGGCCGACGGCTACGGT TACCCCCGCGGCCGGCACATCCTCTCGGTGTTCCCGCTGGCGGCGGGCATCTTCTTCGTG AGGCTGCTCTTCGAGCGATTTATTGCCAAACCCTGTGCACTCTGTATTGGCATCGAGGAC AGTGGTCCTTATCAGGCCCAACCCAATGCCATCCTTGAAAAGGTGTTCATATCTATTACC AAGTATCCTGATAAGAAAAGGCTGGAGGGCCTGTCAAAGCAGCTGGATTGGAATGTCCGA AAAATCCAATGCTGGTTTCGCCATCGGAGGAATCAGGACAAGCCCCCAACGCTTACTAAA TTCTGTGAAAGCATGTGGAGATTCACATTTTATTTATGTATATTCTGCTATGGAATTAGA TTTCTCTGGTCGTCACCTTGGTTCTGGGACATCCGACAGTGCTGGCATAACTATCCATTT CAGCCTCTTTCAAGTGGGCTTTATCACTATTATATCATGGAATTGGCCTTCTATTGGTCC CTTATGTTTTCTCAGTTTACAGACATTAAAAGAAAGGACTTCCTGATCATGTTTGTGCAT CACTTGGTCACCATTGGGCTTATCTCCTTCTCCTACATCAACAATATGGTTCGAGTGGGA ACTCTGATCATGTGTCTACATGATGTCTCAGACTTCTTGCTGGAGGCAGCCAAACTGGCC AATTATGCCAAGTATCAGCGGCTCTGTGACACCCTTTTTGTGATCTTCAGTGCTGTTTTT ATGGTTACACGACTAGGAATCTATCCATTCTGGATTCTGAACACGACCCTCTTTGAGAGT TGGGAGATAATCGGGCCTTATGCTTCATGGTGGCTCCTCAATGGCCTGCTGCTGACCCTA CAGCTTCTGCATGTCATCTGGTCCTACCTAATTGCACGGATTGCTTTGAAAGCCTTGATC AGGGGAAAGGTATCGAAGGATGATCGCAGTGATGTGGAGAGCAGCTCAGAGGAAGAAGAT GTGACCACCTGCACAAAAAGTCCCTGTGACAGTAGCTCCAGCAATGGTGCCAATCGGGTG AATGGTCACATGGGAGGCAGCTACTGGGCTGAAGAGTAA Clone variation with respect to NM_147190.2 >OriGene 5' read for NM_147190 unedited
TTATAACCCCCGCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATA AGCAGAGCTCATTTACGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCC GCGAATTCGGCACGAGGATGGCGACAGCAGCGCAGGGACCCCTAAGCTTGCTGTGGGGCT GGCTGTGGAGCGAGCGCTTCTGGCTACCCGAGAACGTGAGCTGGGCTGATCTGGAGGGGC CGGCCGACGGCTACGGTTACCCCCGCGGCCGGCACATCCTCTCGGTGTTCCCGCTGGCGG CGGGCATCTTCTTCGTGAGGCTGCTCTTCGAGCGATTTATTGCCAAACCCTGTGCACTCT GTATTGGCATCGAGGACAGTGGTCCTTATCAGGCCCAACCCAATGCCATCCTTGAAAAGG TGTTCATATCTATTACCAAGTATCCTGATAAGAAAAGGCTGGAGGGCCTGTCAAAGCAGC TGGATTGGAATGTCCGAAAAATCCAATGCTGGTTTCGCCATCGGAGGAATCAGGACAAGC CCCCAACGCTTACTAAATTCTGTGAAAGCATGTGGAGATTCACATTTTATTTATGTATAT TCTGCTATGGAATTAGATTTCTCTGGTCGTCACCTTGGTTCTGGGACATCCGACAGTGCT GGCATAACTATCCATTTCAGCCTCTTTCAAGTGGGCTNTATCACTATTATATCATGGAAT TGGCCTTCTATTGGTCCCTTATGTTTTCTCAGTTTACAGACATTAAAAGAAAAGACTTCC TGATCATGTTTGTGCATCACTTGGTCACCATTGGGCCTTATCTCCCTCTCCTACATCAAC CATATGGTTCCGAGTGGGGAACTCTGATCATGCGTCTACATGCAGGCTTAGACCTCCTGC CTGGAGCCAGCCAACTGGTCAATTATGCCCAGTATC >OriGene 3' read for NM_147190 unedited
ACCTACTCACATCTGGCTGATGGCAACTTNCCAGGNCCAGNANAGCACTGGGGNAGGGTC ACAGGGATGCCACCCGGGATCTGTTCAGGAAACACTATGACCGCGGCCGCAATCTAGAGT CGAGTTTTTTTTTTTTTTTTTTTGGGTTGAAGCACTTTTAATCACAAACAAACAATAGTA TAAGACCGTGAACAGCTCACCCAGTGGCTGTTGCCCTCCTCCAGCCCTCAGCTCACCCTC TTCCCATGCTCCCTCCCTGGGTACCATTGAGTAAAGAGCAGGGTATTGGCAGCTGTCCCT GTTGCTATCAGGTGATTGATAGGAAGGGGATCTAGCTAGGGACCACGGCAATCCTGGCCA TCCATCCATGGTTCTGTTTCTATACAACTTCATCATGTGACATATTCCAGCTGTGAAAGA GGAACAGTACAGAAATGTGGGACTGAACTTGGCTGCTTCATTCAGATGGTTCATGGGTTT AGAGTGTCAAGAGCCAAGGGCAAAAAAGAAGGAGAAGTCTGGAGTTGGCATAATTCAAGA TGCATCCCAATGTTGTACTAATGTGGGTTCAGTGGTGACCAGGGAAGGCCTCATGGTCCC ATTCCCACAATTGGGGCCCAACCCCGGCAATGAAACTTATGCTTATGCTCTATCGCCCCT CAGCAGATTCACTAAAACTTATAGTGGAAATAACATTACTGAAAGTACCTTGAGGTTTTT ATATACCAATGGCAGTTCTCCTCCAAAGTGAAAATTTTTGGTTTTTTTGATACTCCCTCA TGCGCCTGCAAGGCTTCATATCAGTTGGAGAGCTTTACTGGGCTAGGGCCAGCATCAGTA TTTTTCCACTCTGCCCCACCAAATCCAGCGGCAGCTGAAATCCCCTTAAACTACCGACTT TAATGTTCGTACCC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_147190 |
| Insert Size | 2000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_147190.1, NP_671723.1 |
| RefSeq Size | 2022 bp |
| RefSeq ORF | 1179 bp |
| Locus ID | 91012 |
| UniProt ID | Q8N5B7 |
| Domains | homeobox, LAG1, TLC |
| Protein Families | Transcription Factors, Transmembrane |
| Gene Summary | This gene encodes a protein that belongs to the TLC (TRAM, LAG1 and CLN8 homology domains) family of proteins. The encoded protein functions in the synthesis of ceramide, a lipid molecule that is involved in a several cellular signaling pathways. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013] Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Chemosensitivity of human colon cancer cells is influenced by a p53-dependent enhancement of ceramide synthase 5 and induction of autophagy
,Brachtendorf, S;Wanger, RA;Birod, K;Thomas, D;Trautmann, S;Wegner, MS;Fuhrmann, DC;Brüne, B;Geisslinger, G;Grösch, S;,
Biochim. Biophys. Acta
,PubMed ID 30059758
[CERS5]
|
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A fluorescent assay for ceramide synthase activity
,Hyun Joon Kim, Qiao Qiao, Hamish D. Toop, Jonathan C. Morris, and Anthony S. Don,
J. Lipid Res., Aug 2012; 53: 1701 - 1707.
[CERS5]
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Necessary Role for the Lag1p Motif in (Dihydro)ceramide Synthase Activity
,Stefka Spassieva, Jae-Gu Seo, James C. Jiang, Jacek Bielawski, Fernando Alvarez-Vasquez, S. Michal Jazwinski, Yusuf A. Hannun, and Lina M. Obeid,
J Biol Chem. 2006 Nov 10;281(45):33931-8.
[CERS5]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207649 | CERS5 (Myc-DDK-tagged)-Human LAG1 homolog, ceramide synthase 5 (LASS5) |
CNY 5,488.00 |
|
| RC207649L3 | Lenti-ORF clone of CERS5 (Myc-DDK-tagged)-Human LAG1 homolog, ceramide synthase 5 (LASS5) |
CNY 5,890.00 |
|
| RC207649L4 | Lenti-ORF clone of CERS5 (mGFP-tagged)-Human LAG1 homolog, ceramide synthase 5 (LASS5) |
CNY 5,890.00 |
|
| RG207649 | CERS5 (tGFP-tagged) - Human LAG1 homolog, ceramide synthase 5 (LASS5) |
CNY 7,088.00 |
