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DIRAS1 (NM_145173) Human Untagged Clone
CAT＃: SC100097
DIRAS1 (untagged)-Human DIRAS family, GTP-binding RAS-like 1 (DIRAS1)
CNY 2,400.00
CNY 2,950.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | Di-Ras1; GBTS1; RIG |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC100097 sequence for NM_145173 edited (data generated by NextGen Sequencing)
ATGCCGGAACAGAGTAACGATTACCGCGTGGTGGTGTTCGGGGCGGGCGGCGTGGGCAAG AGCTCGCTGGTGCTGCGCTTCGTGAAGGGCACGTTCCGCGACACCTACATCCCCACCATC GAGGACACCTACCGGCAGGTGATCAGCTGCGACAAGAGCGTGTGCACGCTGCAGATCACA GACACCACCGGCAGCCACCAGTTCCCGGCCATGCAGCGCCTGTCCATCTCCAAGGGCCAC GCCTTCATCCTGGTGTTCTCCGTCACCAGCAAGCAGTCGCTGGAGGAGCTGGGGCCCATC TACAAGCTCATCGTGCAGATCAAGGGCAGCGTGGAGGACATCCCCGTGATGCTCGTGGGC AACAAGTGCGATGAGACGCAGCGGGAGGTGGACACGCGCGAGGCGCAGGCGGTGGCCCAG GAGTGGAAGTGCGCTTTCATGGAGACCTCGGCCAAGATGAACTACAACGTCAAGGAGCTC TTCCAGGAGCTGCTGACGCTGGAGACGCGCCGGAACATGAGCCTCAACATCGACGGCAAG CGCTCCGGCAAGCAGAAGAGGACAGACCGCGTCAAGGGCAAATGCACCCTCATGTGA Clone variation with respect to NM_145173.3 549 g=>c >OriGene 5' read for NM_145173 unedited
CGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGGGGACGGCGCGGGCGCGACTGCGG CGGCTCGGGCGGGCGGGGGGCGGCGGCGCCAGCGGACACCGGAGCGGGCAGGAGCAGCGG CCGCGGCGCCGCAGGGACCAGCGGGCCCAGGCTCGGTCTGGGGTTCTTGCAGCTGGCACC AGCCCTCCCGGCCCCGGCCGCCCACCCGCCGCGGGGAAACATGCCGGAACAGAGTAACGA TTACCGCGTGGTGGTGTTCGGGGCGGGCGGCGTGGGCAAGAGCTCGCTGGTGCTGCGCTT CGTGAAGGGCACGTTCCGCGACACCTACATCCCCACCATCGAGGACACCTACCGGCAGGT GATCAGCTGCGACAAGAGCGTGTGCACGCTGCAGATCACAGACACCACCGGCAGCCACCA GTTCCCGGCCATGCAGCGCCTGTCCATCTCCAAGGGCCACGCCTTCATCCTGGTGTTCTC CGTCACCAGCAAGCAGTCGCTGGAGGAGCTGGGGCCCATCTACAAGCTCATCGTGCAGAT CAAGGGCAGCGTGGAGGACATCCCCGTGATGCTCGTGGGCAACAAGTGCGATGAGACGCA GCGGGAGGTGGACACGCGCGAGGCGCACGCGGTGGCCCAGGAGTGGAAGTGCGCTTTCAT GGAGACCTCGGCCAAGATGAACTACAACGTCAAGGAGCTCTTCCAGNAGCTGCTGACGCT GGAGACGCGCCGGAACATGAGCCTCACATTGACGGCAAGCGCTNCGGCAAGCAGAAGAGA CAGACCGCGTCAGGGGCAAATGCACCCTCATGTGAGCCCGGACGCCCGGCTGGGCCGGTG GCACACCCAACTGACCCCGGTGGTTCCCCGAAACGACACACTTGCTGGACCCTCTGTTTT TTTATCCCCCTGGCCACCTTGGCGGTGAAACCGGGCCACA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_145173 |
| Insert Size | 3500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_145173.1, NP_660156.1 |
| RefSeq Size | 3391 bp |
| RefSeq ORF | 597 bp |
| Locus ID | 148252 |
| UniProt ID | O95057 |
| Domains | ras, RAS, RHO, RAB |
| Gene Summary | DIRAS1 belongs to a distinct branch of the functionally diverse Ras (see HRAS; MIM 190020) superfamily of monomeric GTPases.[supplied by OMIM, Apr 2004] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
RAS-related GTPases DIRAS1 and DIRAS2 induce autophagic cancer cell death and are required for autophagy in murine ovarian cancer cells
,Sutton, MN;Yang, H;Huang, GY;Fu, C;Pontikos, M;Wang, Y;Mao, W;Pang, L;Yang, M;Liu, J;Parker-Thornburg, J;Lu, Z;Bast, RC;,
Autophagy
,PubMed ID 29368982
[DIRAS1]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206160 | DIRAS1 (Myc-DDK-tagged)-Human DIRAS family, GTP-binding RAS-like 1 (DIRAS1) |
CNY 2,400.00 |
|
| RC206160L3 | Lenti ORF clone of Human DIRAS family, GTP-binding RAS-like 1 (DIRAS1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206160L4 | Lenti ORF clone of Human DIRAS family, GTP-binding RAS-like 1 (DIRAS1), mGFP tagged |
CNY 5,890.00 |
|
| RG206160 | DIRAS1 (tGFP-tagged) - Human DIRAS family, GTP-binding RAS-like 1 (DIRAS1) |
CNY 4,000.00 |
