Myo9b (NM_001271066) Rat Tagged ORF Clone
CAT#: RR217725
- TrueORF®
Myo9b (myc-DDK-tagged) - Rat myosin IXb (Myo9b), transcript variant 1
"NM_001271066" in other vectors (1)
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CNY 19,380.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR217725 representing NM_001271066
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGCTCACGAGGCTGGCAGCTCAGGCCGAAGGCGGCCGGCCACCTTCCACCTGCACATCTACCCTC AGCTGCCCAGTGCTGGGAGCCAGACCTCATGCCGTGTAACCGCCACCAAGGACAGCACAACAAGCGATGT CATCCGAGACGTGGTGGCCAGTCTACACCTGGACGGCTCGAAGCACTATGTGCTGGTGGAGGTGAAGGAG TCGGGTGGGGAGGAGTGGGTGCTGGATGCCAGCGACTCGCCTGTGCACCGAGTGCTGCTGTGGCCTCGGC GAGCGCAGAAGGAGCACCCTCGGGAGGACGGCTACTACTTCCTGCTGCAGGAGCGCAACGCTGACGGCAG CATTCAGTACCTGCACGTGCAGCTGCTGGCTCAGCCCACAGCCGCATGTCGCCTGGTGGAGCGAGGGCTG CTGCCAAGGCCTCAAGCGGACTTCGACGACCTGTGCAACCTGCCGGAGCTGAACGAGGCCAACCTGCTGC AGAGCCTGAAGCTGCGCTTCGTGCAGCAGAAGATCTACACGTACGCGGGCAGCATCCTGGTGGCCATCAA CCCCTTCAAGTTCCTGCCCATTTACAACCCCAAGTATGTGAAGATGTACGAGAACCAGCAGCTGGGAAAG CTGGAGCCGCACGTGTTCGCTCTGGCCGATGTAGCCTACTACACCATGCTGCGCAAGCACGTGAACCAGT GCATCGTCATCTCTGGTGAGAGCGGGTCCGGCAAGACGCAAAGCACCAACTTCCTCATCCACTGCCTCAC AGCGCTCAGCCAGAAGGGCTACGCCAGCGGCGTCGAGAGGACCATCCTGGGTGCAGGGCCTGTGCTGGAG GCTTTTGGGAACGCCAAGACAGCCCACAACAACAACTCCAGCCGCTTCGGGAAGTTCATCCAAGTCAACT ACCTGGAGAGTGGCATCGTGAGGGGAGCTGTTGTGGAAAAATACCTTCTTGAAAAGTCTCGCCTGGTTTC CCAGGAGAAGGATGAGCGGAACTACCATGTGTTTTATTATCTGCTGCTGGGCGTCAGTGAGGAAGAGCGT CAGGAATTTCAGCTGAAGCAGCCTCAAGACTATTTCTACCTCAACCAGCATAACTTGAATATTGAAGATG GAGAAGACCTCAAACATGACTTTGAAAGGCTTCAGCAGGCCATGGAGATGGTGGGCTTCCTACCTGCCAC GAAGAAGCAGATCTTCTCTGTCCTCTCAGCCATCCTGTATCTTGGCAATGTCACCTATAAGAAGAGAGCC ACAGGCCGGGATGAAGGCCTGGAGGTCGGGCCCCCGGAGGTATTGGACACACTATCCCAGCTCCTGAAGG TAAAGCGGGAGACCCTGGTGGAGGTCCTAACCAAGAGAAAAACAATCACGGTCAATGACAAACTCATCCT GCCTTACAGCCTCAGTGAGGCTATCACTGCACGAGACTCCATGGCTAAGTCTCTGTACAGCGCCCTGTTT GACTGGATTGTGCTGAGGATCAACCACGCCCTCCTCAACAAGAAGGACATGGAAGAGGCTGTTTCTTGCT TGTCCATTGGCGTCCTGGACATATTCGGATTTGAGGACTTTGAACGGAACAGCTTCGAGCAGTTTTGCAT CAACTACGCCAACGAGCAGTTGCAGTACTACTTCACCCAGCACATCTTCAAGCTGGAGCAGGAGGAGTAC CAGGGTGAGGGTATCTCGTGGCACAACATTGACTACACCGACAACGTGGGCTGTATCCACCTCATCAGCA AGAAGCCCACTGGCCTCTTCTACCTGCTGGACGAGGAAAGCAACTTTCCCCATGCCACAAGCCACACTCT GCTGGCCAAATTCAAGCAGCAGCATGAGGACAACAAGTACTTCCTGGGCACACCAGTCCTGGAGCCCGCC TTCATCATCCAGCACTTCGCAGGCAGAGTAAAGTACCAGATCAAGGACTTCCGGGAGAAGAACATGGACT ACATGCGGCCTGACATCGTGGCACTGCTAAGGGGCAGTGACAGCTCCTATGTGCGCCAACTCATCGGCAT GGACCCGGTAGCTGTGTTCCGCTGGGCTGTATTACGGGCAGCCATCAGGGCCATGGCTGTGCTGCGGGAG GCTGGGCGCCTGCGTGCAGAGAGAGCAGAGAAAGCAGAAGCAGGTGTAAGTAGTCCTGTCACTCGAAGTC ACGTGGAAGAGCTGCCAAGAGGAGCCAACACCCCTTCAGAGAAACTGTACCGCTGCGCAGGGCTAGACTT CTCCTTTGAGCGCTCTGAGGAGCTGGATGTTAACGCTTTTGAGGACATCATGGCTTTCTATGAGAGCAGG AAGGTGAGGGAGCGGAGCGATTTGCATAACCAAATCATCAAGAGCCTCAAAGGACTGCCATGGCAGGGCG AGGACCCGCGGAGGCTTCTCCAGTCCCTCAGTCGGCTCCAGAAGCCCCGCACCTTCTTCCTGAAGAGTAA AGGTATCAAACAAAAGCAGATCATTCCCAAGAACCTGCTGGACTCTAAGTCCCTGAGGCTCATCATCAGC ATGACGCTGCATGACCGAACTACCAAGTCACTGCTGCACCTGCACAAGAAGAAGAAGCCGCCCAGCATCA GTGCACAGTTCCAGACATCTCTTAACAAGCTGTTGGAGGCGCTGGGCAAGGCTGAGCCCTTCTTCATCCG CTGCATTCGCTCCAATGCCGAGAAGAAGGAGCTCTGCTTTGATGATGAGCTGGTGCTTCAGCAACTGCGC TACACAGGCATGCTGGAGACCGTGCGCATCCGGCGCTCTGGCTACAGCGCCAAGTACACCTTCCAGGACT TCACGGAGCAGTTCCAGGTGCTGCTGCCCAAGGATGTCCAGCCCTGTAGGGAGGCCATTGCTGCCCTGTT GGAGAAGCTGCAGGTGGACAGGCAGAACTACCAGATCGGGAAGACGAAGGTCTTCCTGAAGGAGACAGAG CGGCAGGCCCTGCAGGAGAGGCTGCATGGTGAGGTCTTACGCAGGATCCTGCTGCTGCAGAGTTGGTTCC GGATGGTGCTGGAACGCAGGCACTTTGTGCAGATGAAGCATGCTGCCCTGACCATCCAGGCCTGCTGGCG GTCTTATCGTGTGCGCCGTACACTGGAGAGGACGCGGGCAGCTGTGTATCTTCAGGCTGCCTGGAGAGGC TACCTGCAGAGACAGGCCTACCACCACCAGAGGCATAGCATCATCCGCCTGCAGAGCCTCTGCCGTGGCC ACCTACAGCGCAGGAGCTTCAGCCAGATGATGTTAGAGAAGCAGAAGGCAGAGCAAGCCAGGGAGACTGC AGGAGCAGAGATGTCAGAGGGAGAGCCCAGCCCTGTGGCCGCTGGGGAGCAGCCGTCTGAGCACCCTGTG GAGGACCCTGAGAGCCTGGGTGTGGAGACTGAGACCTGGATGAACAGCAAGTCCCCAAATGGCTTGTCAC CTAAGAAGGAGATCCCCAGCCCGGAGATGGAGACCCCAGCCCAAAAAACAGTGCCAGCTGAAAGTCATGA GAAAGTCCCCAGTAGCCGGGAGAAGCGAGAGTCACGGCGGCAGCGAGGGTTGGAGCATGTTGAACGACAG AACAAACACATCCAATCCTGCAGGGAGGAGAATAGCACCCTCCGAGAACCTTCCAGAAAGGCAAGCCTGG AAACAGGGGAGAGCTTCCCTGAGGACACAAAGGAGCCCAGAGAAGATGGACTTGAGACATGGACTGAGAC CGCAGCCCCCTCTTGTCCAAAGCAGGTGCCGATTGTGGGAGATCCGCCTAGGAGTCCCAGTCCCTTGCAG AGGCCCGCCAGCCTGGACCTAGACAGCAGGGTTAGCCCAGTGCTCCCCAGCAGCTCCCTGGAATCCCCCC AGGATGAGGACAAGGGTGAGAACAGCACCAAGGTTCAGGACAAGCCCGAGAGTCCCAGTGGCTCCACCCA GATCCAACGGTACCAACACCCGGACACAGAGCGGCTAGCCACTGCTGTGGAGATATGGCGAGGCAAGAAG CTCGCCAGTGCCATGCTGAGCCAATCCCTGGACCTGAGTGAGAAGCCCCGGACTGCAGGGGCAGCCCTGA CTCCCACAGAGGAGAGGCGCATCTCCTTCTCCACCAGTGATGTCTCCAAGCTGTCCCCGGTCAAGACTTC AACTGAAGTCGATGGGGATCTGAGCGCCAAGAAGCCAGCCGGCCATAAGAAGAAGTCAGAAGACCCATCT GCTGGTCCCGATGCAGGCCTGCCCACAGGCTCCCAGGGTGACTCTAAATCTGCCTTTAAGCGACTCTTCC TGCACAAAGCCAAGGATAAGAAGCCCAGCCTGGAGGGTGTGGAGGAGACAGAGGGCAGTGGAGGGCAGGC TGCACAGGAGGCCCCGGCCAGGAAGACTCTAGATGTACCTTCTAGCCAGCAGCACCGCCACACCACAGGC GAGAAGCCTCTAAAAGGGAAGAAGAACCGAAATCGTAAGGTCGGACAGATCACAGTGTCCGAGAAGTGGC GAGAGTCGGTGTTCCGTAAGATCACTAATGCCAACGAGCTCAAGTTTCTGGATGAGTTCCTGCTCAACAA GGTGAATGACCTTCGCTCACAGAAGACACCCATCGAGAGCTTGTTCATTGAGGCCACTGAGCGTTTCAGG AGCAATATCAAGACCATGTATTCTGTGCCTAACGGGAAGATCCATGTAGGCTACAAGGACTTGATGGAGA ACTACCAGATCGTTGTCAGTAACCTGGCTGCCGAGCGTGGGGAGAAGGACACCAACCTGGTCCTCAATGT CTTCCAGTCACTGCTGGATGAATTCACTCGCAGCTACAACAAGACTGACTTTGAGCCGGTCAAGGGCAAA GCCCAGAAGAAGAAGAGGAAGCAGGAGCGTGCTGTCCAGGAACACAATGGACATGTGTTTGCCAGCTACC AAGTGAACATCCCGCAGTCGTGTGAGCAGTGTCTGTCCTACATCTGGCTCATGGACAAGGCCCTGCTATG CAGTGTGTGCAAGATGACCTGCCACAAGAAATGTGTGCACAAGATTCAGAGCTATTGCTCCTACACTGGA CGGAGGAAGAGTGAGCTGGGTGCCGAACCAGGCCACTTCGGCGTGTGTGTAGACAGCCTGACCAGTGACA AGGCCTCCGTGCCCATTGTGCTGGAGAAGCTTCTGGAACATGTAGAGATGCATGGCCTGTATACCGAGGG TCTTTACCGCAAGTCAGGCGCTGCCAACCGGACACGGGAATTACGCCAGGCTCTGCAGACAGACCCTGCT ACAGTTAAGCTGGAGGACTTCCCCATCCACGCCATCACCGGGGTCCTGAAGCAATGGCTGCGGGAGCTGC CTGAGCCACTCATGACTTTTGCCCAGTATGGAGACTTCCTCAGGGCTGTTGAGCTTCCAGAGAAGCAGGA GCAGCTGGCTGCCATCTATGCAGTCCTGGACCACCTGCCTGAAGCCAACCACACCTCCCTGGAGAGGCTC ATCTTCCACCTTGTCAAAGTGGCCCTGCTTGAAGATGTGAACCGCATGTCTCCGGGAGCTCTAGCTATCA TCTTCGCGCCCTGCCTGCTTCGCTGCCCTGACAACTCCGACCCCCTGACCAGCATGAAGGACGTACTAAA GATTACCACGTGTGTGGAGATGCTCATCAAGGAACAGATGAGGAAATACAAGGTGAAGATGGAGGAGATC AACCACCTGGAGGCAGCTGAGAGCATTGCGTTCCGCAGGCTCTCCCTGCTGAGGCAGAATGCCCCGTGGC CTCTCAAACTGGGGTTTTCGTCTCCCTATGAGGGGGTCCGGACCAAAAGCCCCAGGACCCCAGTGGTCCA AGACTTGGAGGAGCTGGGGGCTCTCCCCGAGGAGGCGGCAGGTGGTGACGAGGACCGGGAAAAGGAGATT CTCATGGAGAGGATTCAGTCCATCAAAGAAGAGAAGGAGGACATCACATATCGACTGCCGGAGCTGGACC CACGGGGTTCTGACGAGGAGAACCTTGACTCAGAGACATCAGCCAGCACTGAGAGCCTGCTGGAGGAGAG GGCCGTGCGGGGGGCCGCAGAAGGTCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR217725 representing NM_001271066
Red=Cloning site Green=Tags(s) MSAHEAGSSGRRRPATFHLHIYPQLPSAGSQTSCRVTATKDSTTSDVIRDVVASLHLDGSKHYVLVEVKE SGGEEWVLDASDSPVHRVLLWPRRAQKEHPREDGYYFLLQERNADGSIQYLHVQLLAQPTAACRLVERGL LPRPQADFDDLCNLPELNEANLLQSLKLRFVQQKIYTYAGSILVAINPFKFLPIYNPKYVKMYENQQLGK LEPHVFALADVAYYTMLRKHVNQCIVISGESGSGKTQSTNFLIHCLTALSQKGYASGVERTILGAGPVLE AFGNAKTAHNNNSSRFGKFIQVNYLESGIVRGAVVEKYLLEKSRLVSQEKDERNYHVFYYLLLGVSEEER QEFQLKQPQDYFYLNQHNLNIEDGEDLKHDFERLQQAMEMVGFLPATKKQIFSVLSAILYLGNVTYKKRA TGRDEGLEVGPPEVLDTLSQLLKVKRETLVEVLTKRKTITVNDKLILPYSLSEAITARDSMAKSLYSALF DWIVLRINHALLNKKDMEEAVSCLSIGVLDIFGFEDFERNSFEQFCINYANEQLQYYFTQHIFKLEQEEY QGEGISWHNIDYTDNVGCIHLISKKPTGLFYLLDEESNFPHATSHTLLAKFKQQHEDNKYFLGTPVLEPA FIIQHFAGRVKYQIKDFREKNMDYMRPDIVALLRGSDSSYVRQLIGMDPVAVFRWAVLRAAIRAMAVLRE AGRLRAERAEKAEAGVSSPVTRSHVEELPRGANTPSEKLYRCAGLDFSFERSEELDVNAFEDIMAFYESR KVRERSDLHNQIIKSLKGLPWQGEDPRRLLQSLSRLQKPRTFFLKSKGIKQKQIIPKNLLDSKSLRLIIS MTLHDRTTKSLLHLHKKKKPPSISAQFQTSLNKLLEALGKAEPFFIRCIRSNAEKKELCFDDELVLQQLR YTGMLETVRIRRSGYSAKYTFQDFTEQFQVLLPKDVQPCREAIAALLEKLQVDRQNYQIGKTKVFLKETE RQALQERLHGEVLRRILLLQSWFRMVLERRHFVQMKHAALTIQACWRSYRVRRTLERTRAAVYLQAAWRG YLQRQAYHHQRHSIIRLQSLCRGHLQRRSFSQMMLEKQKAEQARETAGAEMSEGEPSPVAAGEQPSEHPV EDPESLGVETETWMNSKSPNGLSPKKEIPSPEMETPAQKTVPAESHEKVPSSREKRESRRQRGLEHVERQ NKHIQSCREENSTLREPSRKASLETGESFPEDTKEPREDGLETWTETAAPSCPKQVPIVGDPPRSPSPLQ RPASLDLDSRVSPVLPSSSLESPQDEDKGENSTKVQDKPESPSGSTQIQRYQHPDTERLATAVEIWRGKK LASAMLSQSLDLSEKPRTAGAALTPTEERRISFSTSDVSKLSPVKTSTEVDGDLSAKKPAGHKKKSEDPS AGPDAGLPTGSQGDSKSAFKRLFLHKAKDKKPSLEGVEETEGSGGQAAQEAPARKTLDVPSSQQHRHTTG EKPLKGKKNRNRKVGQITVSEKWRESVFRKITNANELKFLDEFLLNKVNDLRSQKTPIESLFIEATERFR SNIKTMYSVPNGKIHVGYKDLMENYQIVVSNLAAERGEKDTNLVLNVFQSLLDEFTRSYNKTDFEPVKGK AQKKKRKQERAVQEHNGHVFASYQVNIPQSCEQCLSYIWLMDKALLCSVCKMTCHKKCVHKIQSYCSYTG RRKSELGAEPGHFGVCVDSLTSDKASVPIVLEKLLEHVEMHGLYTEGLYRKSGAANRTRELRQALQTDPA TVKLEDFPIHAITGVLKQWLRELPEPLMTFAQYGDFLRAVELPEKQEQLAAIYAVLDHLPEANHTSLERL IFHLVKVALLEDVNRMSPGALAIIFAPCLLRCPDNSDPLTSMKDVLKITTCVEMLIKEQMRKYKVKMEEI NHLEAAESIAFRRLSLLRQNAPWPLKLGFSSPYEGVRTKSPRTPVVQDLEELGALPEEAAGGDEDREKEI LMERIQSIKEEKEDITYRLPELDPRGSDEENLDSETSASTESLLEERAVRGAAEGH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001271066 |
ORF Size | 6048 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001271066.1, NP_001257995.1 |
RefSeq Size | 7092 bp |
RefSeq ORF | 6051 bp |
Locus ID | 25486 |
MW | 229.2 kDa |
Gene Summary | displays ATP-dependent binding to actin: binds members of the rho family of GTPases; may play a role in formation of the actin cytoskeleton [RGD, Feb 2006] |
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FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN217725 | Myo9b (untagged) - Rat myosin IXb (Myo9b), transcript variant 1 |
CNY 19,380.00 |