Lrrk1 (NM_001191624) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217724 representing NM_001191624 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCCGTGTCACAGCGACCACCCAGTATGTACTGGTGTGTGGGGACAGAAGGATCAGTTGTGTGTC CAGGGCCTGCCATGGAGACCCATAATGGTGCCGAAGACATGGGCAGCAAACTGTCTTTACCAGGTGGCAA CTCCCCAGTGCAGAGCCCCAACATGGAAGAAATCCACACAGCATACAAGCAGAGAAACCTCTCCCGAGCC CGGGACCTGCTCAGGGAAGTTTGTGAAGAGAGCGAATCCCCACAGGAAAAGGGCCAGTTGCTGAGCATCT CAGCAGCCCACGGGGATCTAGAGACCGTTCGGTTCCTGCTCACTGAGAAGCGCGTGGAGCTGCCGATGGA GCCCACCGATGACAACCCAGCTGTGGTGGCAGCGCATTTTGGACATGCTGAAGTAGTACACGAATTGCTG GAGTCCTTATCAGGTCCCTGTGCCTCGCAGCGGCTGCTGAACTGGATGCTGGCCCTGGCTTGCCAGCGAG GGCACCTGGAGGTTGTGAAGCTGCTGGTCCTGACGCACGGGGCTGACCCGGAGAACTACGCCGTCAGGAA GAATGAGTTCCCGGTCATTGTGAGGTTGCCCCTCTACGCGGCCATCAAGGCAGGGAATGAAGACATTGCC ATATTCCTGCTTCGGCACGGGGCCTATTTCTGCTCCTACATCCTATTGGATAGCCCTGACCCAAGCAAAC ATCTGCTCAGGAAGTATTTCATCGAGGCCAGCGCCCTGCCCAGCAGCTGTCCCGGGAAAACCGCACTTTG TGTGAAATGGTCCCATCTTAAGTTGCCCTGGGTGGACCTCGACTGGCTCTTAGACATCTCCTGTCAGATC ACAGAGCTTGACCTCTCTGCCAACTGCCTGTCTTCTCTCCCCTCCATCATTCCTTGGGGACTGATCAATC TGAAGAAGCTGAACCTCTCAAACAACCACCTGGGGGAGCTGCCCTGTGTGCAATCATCGGACGAAATCAT CTGCTCCAGGTTAGTTGAAATCGACATCTCCAGTAACAAACTGTCCCACCTCCCGCCTGGATTCTTGCAC CTCTCAAAACTTGAAAGGCTGACTGCTTCCAAAAACTATCTGGAGCGGTTGTTTGAAGAAGAAAATGCCA CAAACTGGATCGGCCTGCGGAAGCTGGAGGAACTCGACCTAGCTGACAACAGACTGACGGAGCTCCCTGT CCAATTTATGCACTCCTTCAAGTCTCTCACCAATCTGAATGTCTCCAGGAACAGCCTCAAGAGCTTTCCA GACCCCTGGTCCTGCCCTCTGAAATGTTGTAAAGCCTCCAAAAATGCTCTGGAATCGCTGCCAGACAAAA TGGCCGTCTTCTGGAAAAACCACCTCAGGGACGTGGATTTCTCCGAGAACTCTCTGAAAGCGGTGCCCCT GGGGGTTTTCCAACTTGATGCCCTCATGTTCTTGAGGTTACAGGGAAACCTGCTGTTGTCACTGCCACAT CATGAGAAGTGGACCTGCAGACAACTCAAGACCCTGGACCTCTCCAGAAACCAACTTGGCAAAAATGAAG ATGGGCTTAAAACAAAGCGGATCTCCCTTTTTACCACCAGAGGGCGCCAGCGTTCTGGAACTGAGACAGC GTCCGTGCTAGAATTCCCAGCTTTTCTAAGCGAGTCTTTGGAGGTCCTTTGTCTGAATGACAACCATCTT GATGCAGTTCCTCCGTCAGTCTGCCTGCTGAAGAACCTCTCAGAGCTCTACTTGGGCAATAACCCTGGTC TCCGAGAGCTCCCTCCGGAGCTAGGCCAGCTGGGAAACCTCTGGCAACTGGACATTGAAGATCTGAACAT TGGCAACGTGCCTGCAGAGGTCAGGAAAGAAGGCCCAAAAGCAACGCTGTCTTTCCTGCGTGCTCAGCTG CGCAAAGCAGAGAAGTGTAAACTGATGAAGATGATCCTCGTGGGCCCCCCACGCCAGGGCAAGTCCACAC TCCTGGAGATCTTACAGACAGGGAAGGCCCCGCAGCCAGCACACAGCGAAGCCACTGTCAGGACCACCAA GTGGGAGCTCCAGAGACCAGCAGGCTCCAAAGCCAAGGTTGAGTCTGTGGAGTTCAACGTCTGGGACATC GGGGGCCCTGCCAGCATGGCCACAGTCAATCAGTGCTTCTTCACAGATAAGGCCCTATATGTGGTGGTCT GGAACCTGGCCCTGGGGGAAGAAGCTGTGGCCAACCTCCAGTTCTGGCTTCTCAACATTGAGGCCAAGGC CCCAAACGCCGTTGTGCTGGTGGTTGGGACACATCTGGACCTTATCGAAGCTAAATTCCGAGTAGAGAGG ATCGCAACACTGCGTGCCTACGTGCTGGCCCTCTGCCGCTCACCATCGGGGTCCAGAGCTACAGGATTCC CAGACATCACTTTCAAACACTTGCACGAGATCTCCTGCAAGAATCTGGAAGGGCAGGAGGGGCTGAGGCA ACTGATCTTCCATGTCACGTGCAACATGAAGGACGTGGGCAGCACTATCGGCTGTCAAAAACTCGCTGGC AGGCTGATCCCCAGGAGCTACATAAGCCTACAGGAGGCTGTGCTGGCTGAACAACAGCGCCGCAGCCTGG AAGATCAAGTACAGTACCTGACGGACAGGCAGCTGGAGCAGCTGGTGGAGCAGACGCCTGGCAATGACAT CAAAGACTATGAGGACCTGCAGTCTGCCATCAGCTTCCTCATAGAAACCGGAACCCTGCTGCACTTCCCA GACACGAGCCATGGCCTAAGGAATCTCTACTTCCTGGATCCCATCTGGCTCTCTGAATGCCTACAGAGGA TCTTTAATATCAAGGGCTCGCGGTCGGTGGCGAAGAATGGGGTGATCCGAGCAGAGGACCTCAGGATGCT GCTGGTGGGGACAGGCTTCACGCAGCAGACGGAGGAGCAATACTTCCAGTTCCTTGCCAAGTTCGAGATT GCCCTTCCGGTTGCCAATGACAGTTACCTCCTACCACACCTCCTCCCATCCAAACCTGGGCTGGACACCC ACAGCATGCGGCACCCAATGGCTAACACCATCCAGCGGGTGTTTAAGATGAGCTTCGTGCCTGTTGGCTT CTGGCAAAGGTTCATAGCACGGATGCTGATCAGCTTGGCTGAGATGGACCTGCAGCTTTTTGAAAACAAG AAGAATACGAAAAGCAGGAACCGGAAAGTCACCATTTACAGCTTTACAGGGAACCAGAGAAACCGCTGCA GCACATTCAGAGTCCGAAGGAATCAGACCATCTACTGGCAGGAAGGGCTGCTGGTCACTTTCGATGGGGG CTACCTCAGTGTGGAATCCTCAGACGTGAACTGGAAAAAGAAAAAAAGCGGAGGGATTAAAATCGTCTGC CAGTCAGAAATGAGGGACTTCTCAGCAATGGCTTTTATCACAGACCACGTCAACTCCCTGATCGACCAGT GGTTCCCCGCCCTGACAGCCACAGAAAGTGACGGGACCCCACTTATGGAGCAGTATGTGCCCTGCCCCGT CTGTGAAGCATCCTGGGCCCAGCATGCAGATCCAAACGAGAGGTCGGAGAGCGTGCAGTACTTCGATATG GAAGATTGTGTGCTCACAGCCATTGAAGGGAACTTCATCTCCTGCCCCAGACACCCAGACCTCCCAGTGC CACTTCAGGAGCTTGTACCAGAGCTGTTTATGACTGACTTCCCAGCCAGGCTTTTCCTGGAGAACAGCAA GCTGGAGCACACAGAGGGTGAGAACAGCATCCTGGGCCAAGGTGGCAGTGGCACAGTCATCTACCAGGCC CAGTACCAGGGCCAGCCTGTGGCTGTGAAGAGATTCCACATCAAGAAGTTCAAGAATTCTGCTAATGCCC CGGCAGACACCATGCTGAGGCACCTGAGAGCCATGGATGCCATGAAGAACTTCTCGGATTTCCGTCAGGA GGCCAGCATGCTGCATGCCTTGCAGCACCCCTGCATTGTGTCACTCATCGGCATCAGCATCCACCCCCTC TGCTTCGCCCTGGAGCTCGCCCCGCTGGGCAGCCTCAACACTGTGCTGTCTGAGAATGCCAAAGATTCTT CCTTTATGCCCCTGGGACACATGCTCACCCAAAAAATAGCCTACCAGATTGCCTCGGGCCTGGCCTACCT GCACAAGAAGAACATCATCTTCTGTGACCTCAAGTCAGACAACATCCTGGTGTGGTCACTGAGCGTCAAG GAGCTCATCAACATCAAACTGTCAGACTACGGCATCTCGAGGCAGTCCTTTCACGAGGGGGCCCTGGGTG TGGAGGGCACCCCAGGCTACCAGGCTCCAGAGATCAGGCCTCGCATTGTGTATGACGAGAAGGTAGACAT GTTCTCCTATGGCATGGTGCTGTATGAGCTGCTCTCAGGACAGCGTCCTGCGCTGGGCCACCACCAACTT CAGATTGTCAAGAAGTTGTCCAAGGGCATCCGCCCAGTGCTGGGGCAGCCAGAGGAAGTGCAATTCTATC GACTGCAGGCGCTCATGATGGAGTGCTGGGACACGAAGCCGGAGAAGCGTCCCCTGGCCCTGTCTGTGGT GAGCCAGATGAAAGACCCAACCTTCGCCACCTTCATGTACATGCTGCCCTGTGGGAAGCAGTCGTCCTTC TTCTCATCCCAGAGCCAGGAGTACACTGTGGTGTTCTGGGATGGGAAGGAGGAGTCAAGGAATTACACGG TGGTCAACACCGAGAAGGGCCTTCTGGAAGTACAGAGGATGACCTGTCCTGGGATGAAGCTGAGCTGTCA GCTCAAGGTCCAGAGCTCAGTGTGGCTAGCCACCGAGGACCAGAAAATCTATATCTACAGCCTTAAGGGT ATGTGCCCATTAAGCATGCCCCAGCAGGCCCTGGACACGCCGGCTGTTGTCACCTGTTTCTTGGCAGTAC CTGTTATTAAAAAGAACTCCTTTCTGGTGCTGGCTGGCCTGGCTGATGGACTCGTGGCTGTGTTTTCTAT GGCACGGGGCACCCCAAAAGAAAGCTGCTCCTACCTGTGCTCGCACACAGCCAACAGGTCCAAGTTCTGC ATCCCAGACGAAGACTCACGGCAGAACCCCTACCCAGTGAGGGCAATGGAAGTGGTCAACAGCGGGTCTG AGGTCTGGTACAGCAATGGGCCAGGCCTCCTCATCATCGACTGTGCCATCCTGGACATCAGCAGGCGGCT GGAGCCCTATGCAGCCCCATCTGTGGTCACATCACTTGTGTGCAGCTCAGACTGCAGAGGAGAGGAGACA GTCTGGTGCCTGGACGACAAGGGCAATTCCTTAGTGATGTACCATTCAGCCACCTACCAGCTGTGTGCCC GGTACTTCTGCGGGGACCCCAGTCCTCTCAGGGACACGTTTTCTGTACAGCCCTCAGTCCCAGAAAGCCC AATCCGTCACATAACCACCTCAAAGGAGCCTGAGGAAGAGTGCATCGCCGACGTGAGCATCATGTACAGC GAGGAGCTGGGCATGCAGATCCTCACCCACCAGGACTCGCTCACTGACTACTGCTCCGTATCCTCGTACT CATCCACACCCCACCCAGACCCCAAGTCCCCCACCAGCCTGCCCAGCTCCCTCACCAGCTATTCCAGTGT GCCTTTCTCCGCCAATTATGAAGACCCAGACAGGCTGCAGGAGCCCAGCGTCACCCCTGACAGAACTGAG CATGATCTGAGCCCCATGGATGGGGAGACGTTCAGCCAACACCTGCAGGCTGTGAAGGTGCTTGCTGTCC AAGACCTCATTTGGGTCCCTAGACATGGTGGAGATATCATTGTCATCGGCCTGGAGAAGGATTCGGGTGC CCAGCGGGGCAGGGTCATCGCTGTTTTAAAAGCCAGAGAGCTCAATCCGCATGGGGTCCTGGTGGATGCG GCAGTGGTGGCAAAGGACACCGTAGTGTGCGGCTTTGCAAACGAAAACACAGAGTGGTGCCTGGCTGTCT GGAGGGGCTGGGGCGCCAGGGAGTTTGACATCTTCTACCAGTCCTATGAGGAGCTGGGCCGGCTGGAGGC GTGTCCTCGCAAGAGAAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217724 representing NM_001191624 Red=Cloning site Green=Tags(s) MAAVSQRPPSMYWCVGTEGSVVCPGPAMETHNGAEDMGSKLSLPGGNSPVQSPNMEEIHTAYKQRNLSRA RDLLREVCEESESPQEKGQLLSISAAHGDLETVRFLLTEKRVELPMEPTDDNPAVVAAHFGHAEVVHELL ESLSGPCASQRLLNWMLALACQRGHLEVVKLLVLTHGADPENYAVRKNEFPVIVRLPLYAAIKAGNEDIA IFLLRHGAYFCSYILLDSPDPSKHLLRKYFIEASALPSSCPGKTALCVKWSHLKLPWVDLDWLLDISCQI TELDLSANCLSSLPSIIPWGLINLKKLNLSNNHLGELPCVQSSDEIICSRLVEIDISSNKLSHLPPGFLH LSKLERLTASKNYLERLFEEENATNWIGLRKLEELDLADNRLTELPVQFMHSFKSLTNLNVSRNSLKSFP DPWSCPLKCCKASKNALESLPDKMAVFWKNHLRDVDFSENSLKAVPLGVFQLDALMFLRLQGNLLLSLPH HEKWTCRQLKTLDLSRNQLGKNEDGLKTKRISLFTTRGRQRSGTETASVLEFPAFLSESLEVLCLNDNHL DAVPPSVCLLKNLSELYLGNNPGLRELPPELGQLGNLWQLDIEDLNIGNVPAEVRKEGPKATLSFLRAQL RKAEKCKLMKMILVGPPRQGKSTLLEILQTGKAPQPAHSEATVRTTKWELQRPAGSKAKVESVEFNVWDI GGPASMATVNQCFFTDKALYVVVWNLALGEEAVANLQFWLLNIEAKAPNAVVLVVGTHLDLIEAKFRVER IATLRAYVLALCRSPSGSRATGFPDITFKHLHEISCKNLEGQEGLRQLIFHVTCNMKDVGSTIGCQKLAG RLIPRSYISLQEAVLAEQQRRSLEDQVQYLTDRQLEQLVEQTPGNDIKDYEDLQSAISFLIETGTLLHFP DTSHGLRNLYFLDPIWLSECLQRIFNIKGSRSVAKNGVIRAEDLRMLLVGTGFTQQTEEQYFQFLAKFEI ALPVANDSYLLPHLLPSKPGLDTHSMRHPMANTIQRVFKMSFVPVGFWQRFIARMLISLAEMDLQLFENK KNTKSRNRKVTIYSFTGNQRNRCSTFRVRRNQTIYWQEGLLVTFDGGYLSVESSDVNWKKKKSGGIKIVC QSEMRDFSAMAFITDHVNSLIDQWFPALTATESDGTPLMEQYVPCPVCEASWAQHADPNERSESVQYFDM EDCVLTAIEGNFISCPRHPDLPVPLQELVPELFMTDFPARLFLENSKLEHTEGENSILGQGGSGTVIYQA QYQGQPVAVKRFHIKKFKNSANAPADTMLRHLRAMDAMKNFSDFRQEASMLHALQHPCIVSLIGISIHPL CFALELAPLGSLNTVLSENAKDSSFMPLGHMLTQKIAYQIASGLAYLHKKNIIFCDLKSDNILVWSLSVK ELINIKLSDYGISRQSFHEGALGVEGTPGYQAPEIRPRIVYDEKVDMFSYGMVLYELLSGQRPALGHHQL QIVKKLSKGIRPVLGQPEEVQFYRLQALMMECWDTKPEKRPLALSVVSQMKDPTFATFMYMLPCGKQSSF FSSQSQEYTVVFWDGKEESRNYTVVNTEKGLLEVQRMTCPGMKLSCQLKVQSSVWLATEDQKIYIYSLKG MCPLSMPQQALDTPAVVTCFLAVPVIKKNSFLVLAGLADGLVAVFSMARGTPKESCSYLCSHTANRSKFC IPDEDSRQNPYPVRAMEVVNSGSEVWYSNGPGLLIIDCAILDISRRLEPYAAPSVVTSLVCSSDCRGEET VWCLDDKGNSLVMYHSATYQLCARYFCGDPSPLRDTFSVQPSVPESPIRHITTSKEPEEECIADVSIMYS EELGMQILTHQDSLTDYCSVSSYSSTPHPDPKSPTSLPSSLTSYSSVPFSANYEDPDRLQEPSVTPDRTE HDLSPMDGETFSQHLQAVKVLAVQDLIWVPRHGGDIIVIGLEKDSGAQRGRVIAVLKARELNPHGVLVDA AVVAKDTVVCGFANENTEWCLAVWRGWGAREFDIFYQSYEELGRLEACPRKRR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191624
ORF Size	6039 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191624.1, NP_001178553.1
RefSeq Size	6445 bp
RefSeq ORF	6042 bp
Locus ID	308703
MW	225.4 kDa

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