Lct (NM_053841) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Lph
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217710 representing NM_053841 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGCCTTGGACAGCACTCTTCCTGAGTACTGTCCTCCTGGGTCTTTCATGCCAGGGCTCTGACT GGGAATCCGACAGAAATTTTATTTCAGCCGCTGGTCCTCTAACAAATGACTTAGTGCTTAATCTGAACTA TCCACCGGGAAAGCAGGGCTCTGATGTGGTATCCGGTAACACCGACATCTACATCTGCCAGCAGCCACTG CCCTCTTTCTTGTCTCAGTATTTCAGCAGTCTTCGTGCCAGCCAGGTTACCCATTACAAAGTTCTTCTGT CATGGGCACAACTTCTCCCAACTGGAAGCTCCAAGAATCCAGACCAGGAAGCAGTGCAGTGCTACCGACA ACTCCTCCAGTCCCTGAAGGATGCCCAGCTTGAGCCCATGGTGGTCCTGTGCCATCAGACGCCACCCACC AGCAGCGCCATCCAGAGAGAAGGGGCCTTTGCTGACCTCTTTGCTGACTATGCCACATTGGCTTTTCAGT CCTTTGGGGACCTTGTTGAGATCTGGTTCACCTTCAGTGACTTGGAGAAAGTGATCATGGATCTTCCCCA CAAAGATCTGAAAGCTTCTGCTCTTCAAACCCTCAGCAATGCCCACAGGAGAGCCTTTGACATTTACCAC AGAAAGTTCTCATCTCAGGGGGGAAAGCTGTCTGTGGTCCTGAAGGCTGAAGATATTCCAGAGCTTCTGC CAGACCCGGCATTGGCTGCTCTCGTCCAGGGCTCGGTGGATTTCCTCTCTCTTGATTTGTCTTACGAATG CCAAAGTGTGGCGACTCTACCACAGAAGCTGAGTGAATTGCAGAACCTTGAGCCAAAAGTGAAAGTTTTC ATCTACACCCTAAAACTTGAGGACTGCCCAGCGACCGGGACGAGCCCCTCCTCACTGCTCATCAGCCTCC TGGAAGCCATAAATAAAGACCAAATCCAAACTGTTGGCTTTGATGTTAATGCATTCCTGAGCTGTACGTC CAATTCTGAGGAGAGCCCATCTTGTTCTTTAACCGACAGCCTGGCCCTTCAGACTGAGCAGCAGCAGGAG ACAGCTGTACCCTCATCCCCTGGATCAGCCTACCAGAGAGTTTGGGCAGCCTTTGCTAACCAGTCTAGGG AGGAAAGGGATGCTTTCCTACAAGATGTGTTTCCAGAAGGGTTCCTCTGGGGCATATCCACGGGAGCCTT TAATGTGGAGGGAGGCTGGGCAGAAGGCGGCAGAGGGCCGAGCATCTGGGATCACTACGGTAACCTGAAT GCTGCCGAGGGACAAGCCACAGCAAAAGTGGCCAGTGACAGTTACCACAAACCAGCTTCTGATGTCGCCC TCCTCCGAGGCATCAGGGCTCAGGTGTACAAGTTCTCCATCTCCTGGTCCCGCCTCTTCCCCACTGGGCA GAAGAGCACCCCCAACCGCCAAGGTGTCGCCTACTACAACAAACTGATCGACCGCCTGCTGGACTCACAC ATCGAGCCCATGGCTACACTGTTCCACTGGGACCTGCCTCAGGCCCTGCAGGAGCAAGGCGGCTGGCAGA ACGAGAGTGTGGTGGAGGCCTTCCTGGACTACGCAGCCTTCTGCTTCTCCACTTTCGGGGACCGTGTGAA ACTGTGGGTGACCTTCCACGAGCCATGGGTGATGAGCTATGCAGGCTATGGTACTGGACAGCACGCACCA GCCATCTCTGACCCAGGAATGGCTTCCTTTAAGGTGGCTCACCTGATCCTCAAGGCACATGCCAGAACTT GGCATCTCTACGACCTTCACCATCGCCTACAGCAGCAGGGGCGCGTGGGCATCGTCCTCAATTCAGACTG GGCGGAGCCCCTGGATAGGAAGAGTCCTCAGGACCTTGCTGCTGCTGAGCGCTTCCTACACTTCATGCTG GGCTGGTTCGCACACCCCATCTTTGTAGATGGGGACTACCCCACTACCCTTCGGGCCCAGATCCAGCACA TAAACCAGCAGTGTGGCCATCCTCTGGCCCAACTCCCAGAGTTCACTGAAGCGGAGAAGCGGCTCCTTAA GGGCTCTGCGGATTTCCTGGGTCTGTCTCATTACACCTCCCGCCTCATCAGCAAGGCTGGGCGGCAGACC TGCACCTCCAGCTACGATAACATCGGAGGCTTCTCCCAGCATGTGGACCCCGAGTGGCCCCAGACTGCAT CTCCCTGGATCCGAGTGGTGCCCTGGGGCATAAGGAGGCTGCTTCGGTTCGCGTCTATGGAGTACACGAA AGGGAAACTTCCAATCTTCTTGGCTGGGAATGGCATGCCAGTAGGGGAAGAAGCGGACCTCTTTGACGAC TCAGTGAGAGTAAACTACTTCAACTTGTACATCAACGAGGTGCTCAAGGCTGTCAAGGAGGATTTGGTGG ATGTCCGTTCATACATTGTTCGTTCCCTCATTGACGGCTATGAAGGACCCCTGGGCTTCAGTCAGAGGTT TGGGCTATACCATGTCAACTTCAATGACAGCAGCAGGCCACGAACTCCCAGGAAATCTGCATACTTCTTC ACCAGCATCATTGAAAAGAATGGTTTTTCAGCCAAGAAAGTCAAAAGAAACCCACTGCCCGTGAGAGCAG ACTTCACCTCCAGAGCCAGGGTCACCGACAGCCTTCCATCGGAGGTGCCCTCCAAGGCTAAAATAGTGTG GGAGAAGTTCTCCAAGCAGCCCAGGTTTGAAAGAGACTTGTTCTATCACGGCACATTCCGGGATGACTTC CTGTGGGGTGTATCCTCTTCAGCCTATCAGATCGAAGGAGGATGGAATGCTGATGGCAAAGGCCCCAGCA TCTGGGATAACTTCACCCACACCCCAGGGAATGGTGTGAAAGACAATGCCACCGGAGACGTAGCGTGCGA CAGCTATCACCAACTGGACGCCGACCTAAACATCCTTCGAACCCTGAAGGTCAAGTCCTATCGATTTTCC ATCTCCTGGTCTCGAATTTTCCCAACAGGAAGAAACAGTACCATCAACAAACAAGGCGTTGATTATTACA ACAGACTGATTGACAGCTTGGTGGACAACAACATCTTCCCCATGGTGACACTGTTTCACTGGGACCTGCC CCAGGCCCTCCAGGATATTGGAGGTTGGGAGAACCCTTCTTTGATTGAGTTGTTTGACAGCTATGCAGAC TTCTGCTTTAAGACCTTTGGTGACAGAGTCAAGTTCTGGATGACCTTTAACGAGCCCTGGTGTCACGTGG TGTTAGGCTACAGTTCAGGGATATTTCCCCCAAGTGTTCAGGAGCCAGGCTGGCTACCTTATAAGGTTAG CCATATCGTCATTAAGGCACATGCTAGGGTCTACCATACTTACGATGAGAAGTACCGCTCGGAGCAGAAA GGTGTCATCTCTCTCAGCCTCAACACTCACTGGGCAGAACCCAAGGACCCAGGGCTCCAAAGGGATGTGG AAGCCGCTGACCGGATGCTGCAGTTTACCATGGGCTGGTTTGCCCACCCGATTTTTAAAAACGGGGACTA TCCAGACGTCATGAAATGGACTGTGGGGAACAGGAGTGAGCTGCAGCACCTGGCCAGCTCCCGCCTGCCT ACCTTCACAGAGGAAGAGAAGAATTACGTCAGAGGCACCGCTGATGTCTTTTGCATCAACACCTACACGT CTGTGTTTGTGCAGCACAGCACTCCCAGGCTGAACCCACCCAGCTATGATGATGACATGGAGTTGAAGCT AATCGAAATGAACTCGTCGACGGGTGTGATGCACCCAGACGTGCCCTGGGGGACGAGGAGGCTGCTGAAC TGGATCAAGGAGGAGTATGGCAACATTCCTATTTATATCACTGAAAATGGACAGGGACTGGAAAACCCCA CACTGGATGACACAGAAAGGATCTTCTACCACAAAACCTATATCAACGAGGCTCTGAAAGCCTACAAGCT GGATGGTGTGGACCTTCGAGGGTACTCTGCCTGGACGCTGATGGACGACTTCGAGTGGCTGCTTGGCTAC ACCATGAGATTTGGATTGTATCACGTTGACTTTAATCATGTGAGCAGACCTCGCACAGCAAGAGCCTCAG CCAGATACTATGCAGAGGTCATTGCCAACAATGGCATGCCCCTGGCCAGGGAAGATGAGTTCCTGTATGG AGAGTTTCCCAAGGGCTTCATCTGGAGTGCGGCTTCTGCTTCATACCAGGTTGAAGGCGCGTGGAGAGCA GATGGCAAAGGACTCAGTATCTGGGACACATTTTCCCACACACCACTGAGGATCGGGAATGATGACAACG GAGACGTGGCATGTGACAGTTACCACAAGATTGCCGAGGATGTGGTGGCCCTTCAGAACCTGGGCGTGTC CCACTATCGCTTCTCCATTGCCTGGTCTAGAATCCTCCCAGACGGAACCACCAAGTTCATCAATGAAGCG GGCCTCAGTTACTATGTTCGATTCATTGATGCCCTGCTGGCTGCTGGCATCACACCACAGGTGACCATTT ATCACTGGGACCTGCCCCAGGCACTGCAAGACGTGGGGGGCTGGGAGAACGAGACAATTGTGCAGCGGTT TAAGGAGTACGCGGATGTGCTCTTCCAGAGGCTGGGGGACAGAGTCAAGTTCTGGATCACTCTGAACGAG CCCTTCGTCATTGCAGCCCAAGGCTACGGCACTGGGGTGTCTGCTCCAGGAATCTCCTTCAGGCCTGGCA CAGCTCCGTACATTGCTGGACACAACCTAATAAAGGCCCACGCTGAGGCCTGGCATCTCTACAACGATGT ATACCGCGCCCGCCAGGGAGGCACCATCTCCATCACGATCAGCAGTGACTGGGCTGAACCCAGGGATCCC ACCAACCAGGGAGACGTGGAGGCAGCCAGGAGATACGTACAGTTTATGGGGGGCTGGTTTGCTCACCCCA TCTTCAAAAACGGAGATTACCCGGAGGTGATGAAGACACGGATCCGCGACAGAAGCCTGGCGGCAGGCCT CAACAAGTCCAGGCTCCCTGAGTTTACGGAGAGTGAGAAGAGTAGGATCAAGGGCACCTTTGACTTTTTC GGGTTCAACCACTACACCACTGTCCTAGCTTACAACCTCGACTACCCGGCTGCCTTCTCCTCCTTTGATG CAGACAGGGGCGTTGCTTCCATTGCAGACAGCTCTTGGCCAGTCTCTGGCTCCTTCTGGCTGAAGGTGAC CCCTTTTGGCTTCCGGAGGATCTTGAACTGGCTGAAGGAGGAGTACAATAATCCTCCGATTTATGTCACC GAGAATGGTGTCTCCAGACGAGGAGAGCCGGAACTCAACGACACCGACAGGATCTACTACCTGCGCAGCT ACATTAACGAAGCCCTCAAGGCTGTACAGGACAAGGTGGACCTTCGAGGCTACACGGTATGGAGCATCAT GGACAACTTTGAATGGGCCACAGGCTTCGCAGAGAGGTTCGGTGTGCACTTTGTGAACCGCTCTGACCCA TCTTTGCCAAGGATCCCCAAGGCATCAGCCAAGTTCTATGCCACCATCGTCCGCTGCAATGGCTTTCCCG ACCCTGCACAAGGACCTCATCCTTGTCTCCAGCAACCAGAAGACGCTGCACCCACTGCCAGTCCCGTGCA GTCGGAAGTGCCCTTCCTGGGGCTGATGCTGGGCATCGCAGAAGCACAGACAGCGCTGTACGTGCTCTTC GCTCTGCTGCTGCTTGGAGCCTGCAGCCTGGCATTTCTGACCTACAAATACTGCCGGCGCTCCAAGCAAG GGAACGCGCAGCCAAGCCAGCACCAGCTGAGTCCAATTTCCTCCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217710 representing NM_053841 Red=Cloning site Green=Tags(s) MELPWTALFLSTVLLGLSCQGSDWESDRNFISAAGPLTNDLVLNLNYPPGKQGSDVVSGNTDIYICQQPL PSFLSQYFSSLRASQVTHYKVLLSWAQLLPTGSSKNPDQEAVQCYRQLLQSLKDAQLEPMVVLCHQTPPT SSAIQREGAFADLFADYATLAFQSFGDLVEIWFTFSDLEKVIMDLPHKDLKASALQTLSNAHRRAFDIYH RKFSSQGGKLSVVLKAEDIPELLPDPALAALVQGSVDFLSLDLSYECQSVATLPQKLSELQNLEPKVKVF IYTLKLEDCPATGTSPSSLLISLLEAINKDQIQTVGFDVNAFLSCTSNSEESPSCSLTDSLALQTEQQQE TAVPSSPGSAYQRVWAAFANQSREERDAFLQDVFPEGFLWGISTGAFNVEGGWAEGGRGPSIWDHYGNLN AAEGQATAKVASDSYHKPASDVALLRGIRAQVYKFSISWSRLFPTGQKSTPNRQGVAYYNKLIDRLLDSH IEPMATLFHWDLPQALQEQGGWQNESVVEAFLDYAAFCFSTFGDRVKLWVTFHEPWVMSYAGYGTGQHAP AISDPGMASFKVAHLILKAHARTWHLYDLHHRLQQQGRVGIVLNSDWAEPLDRKSPQDLAAAERFLHFML GWFAHPIFVDGDYPTTLRAQIQHINQQCGHPLAQLPEFTEAEKRLLKGSADFLGLSHYTSRLISKAGRQT CTSSYDNIGGFSQHVDPEWPQTASPWIRVVPWGIRRLLRFASMEYTKGKLPIFLAGNGMPVGEEADLFDD SVRVNYFNLYINEVLKAVKEDLVDVRSYIVRSLIDGYEGPLGFSQRFGLYHVNFNDSSRPRTPRKSAYFF TSIIEKNGFSAKKVKRNPLPVRADFTSRARVTDSLPSEVPSKAKIVWEKFSKQPRFERDLFYHGTFRDDF LWGVSSSAYQIEGGWNADGKGPSIWDNFTHTPGNGVKDNATGDVACDSYHQLDADLNILRTLKVKSYRFS ISWSRIFPTGRNSTINKQGVDYYNRLIDSLVDNNIFPMVTLFHWDLPQALQDIGGWENPSLIELFDSYAD FCFKTFGDRVKFWMTFNEPWCHVVLGYSSGIFPPSVQEPGWLPYKVSHIVIKAHARVYHTYDEKYRSEQK GVISLSLNTHWAEPKDPGLQRDVEAADRMLQFTMGWFAHPIFKNGDYPDVMKWTVGNRSELQHLASSRLP TFTEEEKNYVRGTADVFCINTYTSVFVQHSTPRLNPPSYDDDMELKLIEMNSSTGVMHPDVPWGTRRLLN WIKEEYGNIPIYITENGQGLENPTLDDTERIFYHKTYINEALKAYKLDGVDLRGYSAWTLMDDFEWLLGY TMRFGLYHVDFNHVSRPRTARASARYYAEVIANNGMPLAREDEFLYGEFPKGFIWSAASASYQVEGAWRA DGKGLSIWDTFSHTPLRIGNDDNGDVACDSYHKIAEDVVALQNLGVSHYRFSIAWSRILPDGTTKFINEA GLSYYVRFIDALLAAGITPQVTIYHWDLPQALQDVGGWENETIVQRFKEYADVLFQRLGDRVKFWITLNE PFVIAAQGYGTGVSAPGISFRPGTAPYIAGHNLIKAHAEAWHLYNDVYRARQGGTISITISSDWAEPRDP TNQGDVEAARRYVQFMGGWFAHPIFKNGDYPEVMKTRIRDRSLAAGLNKSRLPEFTESEKSRIKGTFDFF GFNHYTTVLAYNLDYPAAFSSFDADRGVASIADSSWPVSGSFWLKVTPFGFRRILNWLKEEYNNPPIYVT ENGVSRRGEPELNDTDRIYYLRSYINEALKAVQDKVDLRGYTVWSIMDNFEWATGFAERFGVHFVNRSDP SLPRIPKASAKFYATIVRCNGFPDPAQGPHPCLQQPEDAAPTASPVQSEVPFLGLMLGIAEAQTALYVLF ALLLLGACSLAFLTYKYCRRSKQGNAQPSQHQLSPISSF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_053841
ORF Size	5787 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053841.1, NP_446293.1
RefSeq Size	6117 bp
RefSeq ORF	5790 bp
Locus ID	116569
MW	217.6 kDa
Gene Summary	catalyzes the hydrolysis of pyridoxine-5'-beta-D-glucoside to release pyridoxine; also catalyses the hydrolysis of lactose to D-glucose and D-galactose [RGD, Feb 2006]

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