Bai1 (NM_001170597) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217658 representing NM_001170597 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGGGCCAGGCTGCTGCCCCGGGCCCCATCTGGATCCTTGCTCCACTCCTGCTGTTACTATTGCTAC TGGGGCGCTGGGCACGTGCTGCCTCTGGGGCAGACATAGGGCCTGGGACAGAGCAGTGCACCACATTGGT GCAGGGCAAGTTCTTCGGATACTTCTCCGCGGCTGCTGTGTTTCCAGCCAACGCCTCACGCTGCTCTTGG ACTCTTCGCAACCCGGACCCTCGTCGTTACACGCTCTACATGAAGGTGGCCAAGGCACCTGCACCCTGCA GCGGCCCTGGCCGAGTTCGCACCTACCAGTTCGACTCTTTCTTGGAATCCACGCGCACCTACCTGGGTGT GGAGAGCTTTGACGAAGTTCTGCGGCTCTGCGATCCCTCTGCACCCCTAGCCTTCCTGCAGGCCAGCAAA CAGTTCCTACAGATGCAGCGCCAACAGCCACCCCAAGATGGTGACCTTGGTCCCCAGGGCTCTGGTGACG ACTTCTCTGTGGAGTACCTGGTGGTGGGCAATCGCAACCCCAGCCACGCTGCCTGTCAGATGCTGTGCCG TTGGCTGGATGCCTGTCTAGCTGGCAGCCGCAGCTCACACCCCTGTGGCATCATGCAGACTCCCTGTGCC TGCCTGGGTGGAGAAGCTGGAGACACTGCTTCCAGCCCCTTGGTCCCCCGTGGGGACGTCTGCTTGAGAG ACGGCGTAGCTGGTGGCCCTGAGAACTGCCTCACCAGCCTGACCCAGGACCGAGGTGGGCACGGCTCAGC AGGTGGCTGGAAACTGTGGTCCTTGTGGGGCGAATGTACGCGGGACTGCGGGGGAGGCCTACAAACTCGA ACCCGCACTTGCTCGCCCACCCTTGGCGTGGAGGGCAGAGGCTGTGAGGGCGTTCTGGAAGAAGGCCGCC TGTGCAATCGCAAGGCCTGCGGTCCCACTGGGCGCACCAGCTCACGGAGCCAGTCCCTGAGGTCCACGGA TGCCCGGCGTCGTGAGGAGTTTGGGGATGAGCTGCAACAGTTTGGGTTCCCATCCCCCCAGACTGGTGAC CCAGCAGCTGAGGAGTGGTCCCCATGGAGCGTATGCTCCAGCACCTGTGGCGAGGGATGGCAGACCCGTA CGCGCTTCTGCGTGTCATCCTCGTACAGTACTCAGTGCAGTGGACCTCTGAGGGAGCAGCGGCTCTGCAA CAACTCAGCAGTGTGTCCAGTGCATGGAGCCTGGGATGAGTGGTCACCCTGGAGCCTCTGCTCCAGTACC TGTGGCCGTGGCTTCCGTGACCGTACTCGCACTTGCAGGCCCCCTCAGTTTGGAGGCAACCCCTGTGAGG GTCCGGAGAAGCAAACCAAGTTCTGCAACATTGCCCTGTGCCCTGGCCGGGCAGTGGACGGAAACTGGAA TGAATGGTCCAGCTGGAGCACCTGCTCTGCCAGTTGCTCCCAAGGCCGCCAGCAACGGACCCGGGAGTGC AATGGTCCTTCCTATGGGGGTGCCGAGTGCCAGGGCCACTGGGTGGAGACTCGAGACTGCTTCCTGCAGC AATGCCCAGTGGACGGCAAATGGCAGGCCTGGGCATCATGGGGCAGCTGCAGCGTCACGTGTGGAGGTGG AAGCCAGCGACGGGAGCGTGTCTGCTCTGGACCTTTCTTTGGGGGAGCAGCCTGCCAGGGCCCCCAAGAT GAGTACCGACAGTGTGGCGCTCAGCGATGTCCCGAGCCCCATGAGATTTGTGATGAGGACAACTTTGGGG CCGTGGTCTGGAAGGAGACCCCAGCTGGAGAGGTGGCTGCAGTCCGGTGTCCCCGCAATGCCACAGGCCT TATCCTGCGCCGCTGTGAGCTGGACGAGGAGGGCATCGCCTTCTGGGAGCCACCCACCTACATCCGCTGT GTCTCCATTGACTACCGGAACATCCAGATGATGACCCGAGAGCACCTGGCGAAGGCGCAGCGGGGGCTGC CAGGGGAGGGGGTCTCAGAGGTCATCCAGACACTGCTGGAGATCTCACAGGATGGCACCAGCTACAGCGG CGACCTGCTCTCCACCATCGATGTCCTGAGGAACATGACAGAGATCTTCCGCAGGGCTTACTATAGCCCC ACACCCGGGGATGTGCAGAACTTTGTCCAGATCATCAGCAACCTGCTGGCGGAGGAGAACCGGGACAAGT GGGAGGAGGCACAGCTGATGGGCCCCAACGCCAAGGAGCTCTTTCGACTGGTGGAAGACTTTGTGGATGT CATCGGCTTCCGCATGAAGGATTTGAGGGATGCCTACCAGGTGACAGACAATCTGGTGCTCAGCATCCAC AAGCTTCCAGCCAGTGGGGCCACCGACATCAGTTTCCCTATGAAGGGCTGGCGTGCCACTGGAGACTGGG CCAAGGTGCCAGAGGACAGGGTCACCGTGTCCAAGAGTGTGTTTTCCACAGGGCTGGCAGAAGCTGACGA TTCATCTGTGTTCGTGGTTGGTACTGTGCTCTACCGGAACTTGGGCAGCTTCCTAGCCCTGCAGAGGAAC ACAACTGTCCTCAACTCTAAGGTCATCTCCGTGACTGTGAAGCCCCCACCTCGATCGCTCCTCACACCCT TGGAGATCGAGTTCGCCCACATGTACAATGGCACCACCAACCAGACTTGTATCCTGTGGGATGAGACAGA TGGACCCTCCTCCTCCGCCCCCCCGCAGCTCGGGCCCTGGTCGTGGCGCGGCTGCCGCACGGTGCCCCTC GATGCCCTCCGGACGCGCTGCCTGTGTGACCGGCTCTCCACCTTCGCCATCTTAGCCCAGCTCAGCGCCG ACGCGACCATGGATAAGGTGACCGTGCCATCTGTGACGCTCATCGTGGGCTGTGGCGTGTCTTCCCTCAC CCTGCTCATGCTGGTCATCATCTACGTGTCTGTGTGGAGGTACATTCGCTCAGAGCGGTCCGTCATCCTC ATCAACTTCTGCCTGTCCATCATCTCCTCTAATGCCCTCATTCTCATTGGGCAGACCCAGACCCGCAACA AGGTTGTGTGCACACTGGTGGCTGCCTTTTTGCACTTCTTCTTCCTGTCCTCATTCTGCTGGGTGCTCAC CGAGGCCTGGCAATCCTACATGGCTGTCACCGGCCGCCTCCGGAGCCGGCTCGTCCGCAAGCGCTTTCTC TGCCTGGGCTGGGGGCTCCCTGCGCTGGTGGTGGCCATTTCTGTGGGATTCACCAAGGCCAAGGGGTACA GCACCATGAACTATTGCTGGCTCTCCCTGGAGGGGGGACTTCTGTATGCCTTCGTGGGACCGGCTGCTGC AGTTGTGCTGGTGAACATGGTGATCGGGATCCTGGTGTTCAACAAGCTCGTGTCCAAGGACGGCATCACG GACAAGAAGCTGAAGGAGCGGGCAGGGGCCTCCCTGTGGAGCTCCTGCGTGGTGCTGCCGCTGCTGGCGC TGACCTGGATGTCTGCTGTGCTTGCTGTCACCGACCGCCGCTCCGCCCTCTTCCAGATCCTCTTCGCTGT CTTCGACTCACTGGAGGGCTTCGTCATCGTGATGGTGCACTGCATTCTGCGAAGAGAGGTCCAGGATGCT GTGAAGTGTCGTGTGGTAGATCGACAAGAGGAGGGCAACGGGGACTCGGGGGGCTCCTTCCAGAACGGCC ACGCTCAGCTCATGACCGACTTTGAGAAGGATGTGGATCTGGCCTGTAGATCAGTGCTGAACAAAGACAT TGCAGCCTGCCGCACGGCAACTATCACAGGTACCTTCAAGCGACCATCACTGCCTGAAGAGGAGAAGATG AAGCTGGCCAAGGGCCCACCCCCCACCTTCAACAGCCTACCAGCTAATGTGTCCAAGCTGCACCTGCACG GTTCACCCCGCTACCCCGGTGGGCCACTGCCTGACTTCCCCAACCATTCACTCACCCTTAAAAAGGACAA GGCCCCCAAGTCCTCTTTTATTGGTGATGGGGACATCTTTAAGAAACTGGACTCTGAGCTGAGCCGGGCC CAGGAGAAGGCTCTGGACACGAGCTACGTGATCCTGCCCACGGCCACGGCCACACTCCGGCCCAAACCCA AGGAGGAACCCAAATACAGCATCAACATTGACCAGATGCCCCAAACCCGACTCATCCACCTTAGCATGGC GCCCGACGCCAGCTTCCCTACCCGAAGCCCACCTGCCCGTGAGCCCCCAGGTGGTGCACCCCCTGAGGTG CCCCCTGTCCAGCCACCACCACCACCTCCACCACCCCCTCCACCCCAACAGCCCATACCCCCACCACCCT CCCTGGAGCCTGCACCTCCCAGCCTGGGGGACACAGGGGAGCCTTCGGCCCACCCAGGACCCAGTTCAGG TGCTGGGACCAAGAATGAGAACGTGGCCACCTTGTCAGTGAGCTCCTTGGAGCGGCGGAAATCACGGTAT GCAGAACTGGACTTCGAGAAGATCATGCACACACGGAAGCGACACCAGGACATGTTCCAGGACCTGAACC GGAAGCTGCAGCATGCGGCTGAGAAGGAGAAGGAGGTGCCAGGCGTAGACAACAAGCCAGAGAAGCAGCA AACTCCCAACAAGAGGGCCTGGGAGAGCCTCCGCAAGCCCCATGGGACACCCGCTTGGGTAAAGAAGGAG CTGGAGCCACTGCCCCCGTCTCCATTGGAGCTGCGGAGTGTGGAGTGGGAGAAGGCGGGTGCCACCATCC CACTGGTTGGCCAGGACATCATCGACCTCCAGACCGAGGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217658 representing NM_001170597 Red=Cloning site Green=Tags(s) MRGQAAAPGPIWILAPLLLLLLLLGRWARAASGADIGPGTEQCTTLVQGKFFGYFSAAAVFPANASRCSW TLRNPDPRRYTLYMKVAKAPAPCSGPGRVRTYQFDSFLESTRTYLGVESFDEVLRLCDPSAPLAFLQASK QFLQMQRQQPPQDGDLGPQGSGDDFSVEYLVVGNRNPSHAACQMLCRWLDACLAGSRSSHPCGIMQTPCA CLGGEAGDTASSPLVPRGDVCLRDGVAGGPENCLTSLTQDRGGHGSAGGWKLWSLWGECTRDCGGGLQTR TRTCSPTLGVEGRGCEGVLEEGRLCNRKACGPTGRTSSRSQSLRSTDARRREEFGDELQQFGFPSPQTGD PAAEEWSPWSVCSSTCGEGWQTRTRFCVSSSYSTQCSGPLREQRLCNNSAVCPVHGAWDEWSPWSLCSST CGRGFRDRTRTCRPPQFGGNPCEGPEKQTKFCNIALCPGRAVDGNWNEWSSWSTCSASCSQGRQQRTREC NGPSYGGAECQGHWVETRDCFLQQCPVDGKWQAWASWGSCSVTCGGGSQRRERVCSGPFFGGAACQGPQD EYRQCGAQRCPEPHEICDEDNFGAVVWKETPAGEVAAVRCPRNATGLILRRCELDEEGIAFWEPPTYIRC VSIDYRNIQMMTREHLAKAQRGLPGEGVSEVIQTLLEISQDGTSYSGDLLSTIDVLRNMTEIFRRAYYSP TPGDVQNFVQIISNLLAEENRDKWEEAQLMGPNAKELFRLVEDFVDVIGFRMKDLRDAYQVTDNLVLSIH KLPASGATDISFPMKGWRATGDWAKVPEDRVTVSKSVFSTGLAEADDSSVFVVGTVLYRNLGSFLALQRN TTVLNSKVISVTVKPPPRSLLTPLEIEFAHMYNGTTNQTCILWDETDGPSSSAPPQLGPWSWRGCRTVPL DALRTRCLCDRLSTFAILAQLSADATMDKVTVPSVTLIVGCGVSSLTLLMLVIIYVSVWRYIRSERSVIL INFCLSIISSNALILIGQTQTRNKVVCTLVAAFLHFFFLSSFCWVLTEAWQSYMAVTGRLRSRLVRKRFL CLGWGLPALVVAISVGFTKAKGYSTMNYCWLSLEGGLLYAFVGPAAAVVLVNMVIGILVFNKLVSKDGIT DKKLKERAGASLWSSCVVLPLLALTWMSAVLAVTDRRSALFQILFAVFDSLEGFVIVMVHCILRREVQDA VKCRVVDRQEEGNGDSGGSFQNGHAQLMTDFEKDVDLACRSVLNKDIAACRTATITGTFKRPSLPEEEKM KLAKGPPPTFNSLPANVSKLHLHGSPRYPGGPLPDFPNHSLTLKKDKAPKSSFIGDGDIFKKLDSELSRA QEKALDTSYVILPTATATLRPKPKEEPKYSINIDQMPQTRLIHLSMAPDASFPTRSPPAREPPGGAPPEV PPVQPPPPPPPPPPPQQPIPPPPSLEPAPPSLGDTGEPSAHPGPSSGAGTKNENVATLSVSSLERRKSRY AELDFEKIMHTRKRHQDMFQDLNRKLQHAAEKEKEVPGVDNKPEKQQTPNKRAWESLRKPHGTPAWVKKE LEPLPPSPLELRSVEWEKAGATIPLVGQDIIDLQTEV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001170597
ORF Size	4731 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001170597.2, NP_001164068.2
RefSeq Size	5543 bp
RefSeq ORF	4734 bp
Locus ID	362931
UniProt ID	C0HL12
MW	172.9 kDa
Gene Summary	Phosphatidylserine receptor which enhances the engulfment of apoptotic cells (By similarity). Also mediates the binding and engulfment of Gram-negative bacteria (By similarity). Stimulates production of reactive oxygen species by macrophages in response to Gram-negative bacteria, resulting in enhanced microbicidal macrophage activity (By similarity). In the gastric mucosa, required for recognition and engulfment of apoptotic gastric epithelial cells (By similarity). Promotes myoblast fusion (By similarity). Activates the Rho pathway in a G-protein-dependent manner (By similarity). Inhibits MDM2-mediated ubiquitination and degradation of DLG4/PSD95, promoting DLG4 stability and regulating synaptic plasticity (By similarity). Required for the formation of dendritic spines by ensuring the correct dendritic localization of PARD3 and TIAM1 (PubMed:23595754). Potent inhibitor of angiogenesis in brain and may play a significant role as a mediator of the p53/TP53 signal in suppression of glioblastoma (By similarity).[UniProtKB/Swiss-Prot Function]

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