Dip2a (NM_001191564) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217657 representing NM_001191564 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGACCGCGGATGCCCTCTGGAGGCGGCGCCGCTGCCCGCCGAGGTGCTCGAGAGCCTGGCGGAGC TGGAGCTGGAGCTGTCCGAAGGTGACATCACTCAGAAAGGATATGAGAAGAAGAGGGCGAAGCTGCTGGC TCGTTATATCCCCCTCATTCAAGGAGTGGACCCCTGTCTGCAAACAGAGAGTAGAGTCCCTGGGCCCCTG CTGACTGCACCTACAGCTAAACCACAGAAGCCTCGGGCCAACTCGAGGGATGAGCGGTTCCGGTCAGATG TTCACACGGAAGCCGTGCAGGCAGCTCTGGCCAAGTACAAGGAGAGGAAGATGCCCATGCCTTCAAAGAG ACGGTCTGCCCTTGTGCACTCATCCGTGGAAACCTATACACCTCCAGACACGTCATCTGCCTCAGAAGAT GAGGGCTCTTTACGGCGACCTGGGCGACTCACCTCCACTCTGCTCCAGAGCCATTCCGGCATCGAGCCCT GGCTCGACAGGGTCATTCAGGGCTCGTCCACTTCATCCTCTGCATCCTCCACCTCATCCCACCCGGGAGG GAGACCTGCTGCTGCTCCCAGTGCCTCCACTGCGCTTGCAGGCCTCACGGCCCACGCCCACATAGATCCG CACTCCGCCCCACCCGATGTTACCACTGGGCTTGTGGAACACTCACACTTCGAGCGTCCACAGATGGCCT CTGTACGAGGTGTTCCTCGAGGGCACGGCAGGAACATGCTGGAGACCGCAGATGGTGTGCCTGTGAACAG CAGAGTGTCCTCCAAAATCCAGCAGCTATTGAATACCCTGAAGAGGCCAAAACGCCCTCCTCTGAAGGAG TTTTTTGTGGATGATTTTGAGGAGTTACTGGAAGTTCAGCAACCGGATCCCAATCAGCCCAAGCCGGAGG GGGAGCAGATGGCTGTGCTGAAGGGAGAACCTCTGTCAGTGGGGACTTCGGGGCCACCAACTCTGTTGGC TGCACTGCAGCTGTGGGGCACAACACAGCCTAAGGCCCCCTGCCTGACTGCCTTGGATACATCGGGGAAA GCCATCTGCACCCTCACCTATGGCAAACTCTGGAGTCGGAGCTTAAAATTAGCTTATACTCTGCTCAGTA AGCTGACTAGTAAGAATGAGCCACTGCTTAACCCTGGAGACAGGGTGGCACTCGTGTTTCCAAATAGTGA CCCTGTAATGTTTATGGTTGCGTTTTACGGGTGTCTCCTGGCGGAGCTGGTCCCTGTCCCTATAGAAGTA CCATTAACGAGAAAGGATGCAGGCAGCCAGCATGTTGGCTTTCTCCTGGGCAGCTGCGGGGTGACCCTGG CCCTGACCACAGATGCTTGTCAGAAGGGCCTGCCCAAGGCGCCGACAGGAGAGGTGGCAACTTTCAAAGG TTGGCCCCCCCTGGCCTGGCTGGTGATTGATGGGAAGCATCTGACCAAGCCTCCGAAGGACTGGTACCCG CTGGCCCAGGACACAGGGTCCAGGACCGCCTACATTGAGTATAAAACCAGCAAGGACGGCAGCACAGTAG GGGTCACCGTACCCCACTCATCCCTGCTGGCGCAGTGCCAGGCCCTGACCCAGGTGTGTGGGTACACGGA AGCCGAAACATTAACGAATGTGCTGGACTTCAAAAGGGATGCTGGTCTGTGGCATGGAGTCTTAACAAGT GTCATGAACAGGATGCACGTTATCAGCATCCCCTACGCCCTAATGAAGGTCAACCCCCTCTCCTGGATCC AGAAAGTGTGTTCCTATAAAGCCCGGGCTGCCCTGGTGAAGTCCCGAGATATGCACTGGTCACTCTTGGC ACAGCGAGGTCAGAGGGACGTCTGTCTCAGCTCTCTGCGTATGTTGATTGTGGCGGATGGCGCAAACCCA TGGTCAATATCTTCCTGCGATGCCTTCCTCAACGTCTTCCAGTCTAGAGGTCTGAGGCCAGAGGTCATCT GTCCTTGTGCCAGTTCTCCTGAGGCGCTAACAGTAGCCATCCGCAGGCCCCCAGACCTGGGAGGACCTCC ACCAAGGAAAGCTGTCCTGTCCATGAACGGCCTGAGCTACGGTGTGATCAGAGTTGACACCGAGGAGAAG CTGTCGGTCCTCACTGTTCAGGATGTCGGCCAGGTGATGCCTGGAGCTAGTGTCTGTGTCGTGAAGGTAG ACGGTGTCCCTTACCTCTGTAAAACTGATGAAATCGGAGAGATCTGTGTCAGTTCTGTGGCCACTGGGAC AGCATATTACGGGCTGCTTGGCATCACAAAGAATGTCTTTGAGACCGTCCCGGTCACTGCGGATGGAGTC CCTGTCTCTGACCGGCCTTTCACCCGGACAGGTCTTCTGGGGTTTATTGGGCCTGAGAACCTGGTCTTCG TCGTGGGCAAGCTGGACGGGCTGACGGTCGTAGGAGCACGCAGACATAATGCAGATGACATTGTGGCTAC TGCCCTGGCTGTGGAACCCATGAAGTTTGTCTACAGAGGCAGGATCGCCGTGTTCTCTGTGACTGTTCTG CACGATGACCGGATTGTCTTGGTGGCCGAACAGCGGCCCGACTCTTCGGAGGAAGACAGCTTCCAGTGGA TGAGCCGTGTGCTACAGGCCATTGACAGCATCCACCAGGTGGGCGTGTACTGTTTGGCCCTGGTTCCCGC CAACACCTTGCCCAAGGCTCCTCTTGGAGGAATTCACATTTCTGAAACGAAGCAACGCTTCCTGGAAGGG ACGCTACACCCATGTAACGTGTTGATGTGCCCTCACACATGCGTTACCAACCTTCCCAAGCCTCGCCAGA AACAGCCAGAAGTCGGACCAGCCTCCATGATTGTTGGGAACCTGGTTGCCGGAAAGAGGATCGCACAGGC CTCTGGGAGAGAGCTGGCCCACCTGGAGGACAGTGACCAGGCTCGCAAGGTACGTGCGAGGCTTCCTCTC TCTCTGCAGTTCCTGTTCCTGGCGGACGTGCTGCAGTGGAGGGCTCACACCACTCCCGACCACCCGCTGT TCTTGCTCCTGAATGCTAAGGGCACAGTCACCAGCACGGCAACCTGCATCCAGCTGCACAAAAGGGCTGA GAGAGTGGCTGCCGCGCTGATGGAGAAGGGCCGACTAGATGCTGGGGACCATGTGGCTTTGGTGTATCCT CCAGGGGTGGATCTTATTGCTGCGTTCTATGGCTGCCTGTACTGTGGCTGTGTGCCCGTCACTGTGCGAC CCCCACACCCCCAGAACCTAGGCACCACACTGCCCACTGTGAAGATGATTGTCGAGGTCAGCAAATCTGC ATGTGTCCTTAGCACACAGGCCATTACACGGCTGCTCAAGTCCAAAGAAGCAGCTGCGGCTGTAGATGTC AGGACCTGGCCAACCATCCTAGATACAGATGACATTCCAAAAAAGAAGGTAGCTAGCATTTTCAGACCAC CCTCCCCAGATGTGCTTGCATACTTGGACTTCAGTGTGTCGACGACAGGGATCTTAGCGGGAGTTAAGAT GTCACACGCAGCCACAAGTGCCTTGTGCCGCTCTATAAAGCTTCAGTGTGAGCTGTACCCCTCACGGCAG ATTGCCATCTGCCTGGACCCCTACTGTGGCCTTGGCTTCGCCCTGTGGTGTCTGTGCAGCGTGTACTCTG GACACCAGTCGGTACTCGTCCCCCCACTGGAGCTGGAGAGCAATGTGTCTCTCTGGCTGTCCGCCGTCAG CCAGTACAAGGCCCGAGTCACCTTCTGTTCCTACTCAGTGATGGAAATGTGCACCAAGGGCCTTGGTGCT CAGACAGGCGCCCTCAGGATGAAGGGGGTGAACCTGTCGTGTGTGCGCACGTGCATGGTGGTGGCTGAGG AGCGGCCCCGGATCTCGTTGACACAGTCCTTCTCCAAGCTATTCAAAGACCTGGGCCTCCCTGCTCGCGC TGTGAGCACCACCTTCGGGTGCAGGGTCAACGTGGCCATCTGCCTCCAGGGCACAACAGGCCCAGATCCC ACGACGGTCTATGTGGACATGCGGGCACTGCGCCATGACAGAGTTCGTCTGGTGGAACGGGGTTCTCCGC ACAGTCTGCCGCTGATGGAGTCTGGGAAGATTCTTCCAGGAGTGAAGGTCATCATCGCACACACTGAGAC CAAAGGGCCCCTCGGAGACTCACACCTGGGTGAGATCTGGGTGAGCAGCCCCCACAATGCCACTGGGTAC TACACGGTCTATGGGGAGGAGGCACTTCATGCTGACCACTTCAGTGCCCGGCTGAGCTTTGGAGACACCC AGACCATTTGGGCAAGGACTGGCTACCTTGGCTTCCTTCGCAGGACAGAGCTGACTGATGCCAGTGGAGA GCGACACGATGCGCTGTATGTTGTCGGCTCTCTGGACGAGACGCTGGAGCTGAGGGGCATGCGCTATCAC CCCATCGACATCGAGACCTCTGTGATCCGGGCACACAGGAGCATCGCGGAGTGTGCCGTGTTCACCTGGA CCAACCTGCTGGTGGTGGTGGTGGAGCTCGATGGTCTGGAGCAGGATGCCCTGGACCTGGTGGCCCTGGT GACCAATGTCGTGCTGGAGGAGCACTACCTTGTGGTGGGCGTGGTGGTCATTGTGGACCCTGGAGTGATC CCCATCAACTCCCGGGGCGAGAAGCAGCGCATGCACCTGCGCGACGGCTTCCTGGCCGACCAGCTGGACC CCATCTATGTGGCCTACAATATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217657 representing NM_001191564 Red=Cloning site Green=Tags(s) MADRGCPLEAAPLPAEVLESLAELELELSEGDITQKGYEKKRAKLLARYIPLIQGVDPCLQTESRVPGPL LTAPTAKPQKPRANSRDERFRSDVHTEAVQAALAKYKERKMPMPSKRRSALVHSSVETYTPPDTSSASED EGSLRRPGRLTSTLLQSHSGIEPWLDRVIQGSSTSSSASSTSSHPGGRPAAAPSASTALAGLTAHAHIDP HSAPPDVTTGLVEHSHFERPQMASVRGVPRGHGRNMLETADGVPVNSRVSSKIQQLLNTLKRPKRPPLKE FFVDDFEELLEVQQPDPNQPKPEGEQMAVLKGEPLSVGTSGPPTLLAALQLWGTTQPKAPCLTALDTSGK AICTLTYGKLWSRSLKLAYTLLSKLTSKNEPLLNPGDRVALVFPNSDPVMFMVAFYGCLLAELVPVPIEV PLTRKDAGSQHVGFLLGSCGVTLALTTDACQKGLPKAPTGEVATFKGWPPLAWLVIDGKHLTKPPKDWYP LAQDTGSRTAYIEYKTSKDGSTVGVTVPHSSLLAQCQALTQVCGYTEAETLTNVLDFKRDAGLWHGVLTS VMNRMHVISIPYALMKVNPLSWIQKVCSYKARAALVKSRDMHWSLLAQRGQRDVCLSSLRMLIVADGANP WSISSCDAFLNVFQSRGLRPEVICPCASSPEALTVAIRRPPDLGGPPPRKAVLSMNGLSYGVIRVDTEEK LSVLTVQDVGQVMPGASVCVVKVDGVPYLCKTDEIGEICVSSVATGTAYYGLLGITKNVFETVPVTADGV PVSDRPFTRTGLLGFIGPENLVFVVGKLDGLTVVGARRHNADDIVATALAVEPMKFVYRGRIAVFSVTVL HDDRIVLVAEQRPDSSEEDSFQWMSRVLQAIDSIHQVGVYCLALVPANTLPKAPLGGIHISETKQRFLEG TLHPCNVLMCPHTCVTNLPKPRQKQPEVGPASMIVGNLVAGKRIAQASGRELAHLEDSDQARKVRARLPL SLQFLFLADVLQWRAHTTPDHPLFLLLNAKGTVTSTATCIQLHKRAERVAAALMEKGRLDAGDHVALVYP PGVDLIAAFYGCLYCGCVPVTVRPPHPQNLGTTLPTVKMIVEVSKSACVLSTQAITRLLKSKEAAAAVDV RTWPTILDTDDIPKKKVASIFRPPSPDVLAYLDFSVSTTGILAGVKMSHAATSALCRSIKLQCELYPSRQ IAICLDPYCGLGFALWCLCSVYSGHQSVLVPPLELESNVSLWLSAVSQYKARVTFCSYSVMEMCTKGLGA QTGALRMKGVNLSCVRTCMVVAEERPRISLTQSFSKLFKDLGLPARAVSTTFGCRVNVAICLQGTTGPDP TTVYVDMRALRHDRVRLVERGSPHSLPLMESGKILPGVKVIIAHTETKGPLGDSHLGEIWVSSPHNATGY YTVYGEEALHADHFSARLSFGDTQTIWARTGYLGFLRRTELTDASGERHDALYVVGSLDETLELRGMRYH PIDIETSVIRAHRSIAECAVFTWTNLLVVVVELDGLEQDALDLVALVTNVVLEEHYLVVGVVVIVDPGVI PINSRGEKQRMHLRDGFLADQLDPIYVAYNM TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191564
ORF Size	4713 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191564.1, NP_001178493.1
RefSeq Size	5445 bp
RefSeq ORF	4716 bp
Locus ID	690211
MW	170.5 kDa

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