Wdr62 (NM_001191623) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1306714
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217654 representing NM_001191623 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGCTGCCTTAGCGGCCGGAGGTTACGCGCGGAGTGACACGATAGAAAAGCTGTCCTCTGTCATGG CGGGAGTTCCGGCGCGGAGAAACCAGTCCTCCCCGCCTCCTGCCCCACCGCTCTGCCTCCGGCGGCGGAC GCGACTCGCGGCGGCTCCCGAGGACACTGTGCAGAACCGGGTGACACTTGAGAAGGTGCTTGGCATCACA GCCCAGAACAGCAGCGGGCTCACCTGTGACCCTGGCACAGGCCATGTGGCCTACTTAGCAGGCTGTGTGG TGGTGGTCTTGAACCCCAAGGAGAACAAGCAGCAACATATATTTAACACTACCAGGAAGTCCCTGAGTGC TCTGGCCTTCTCCCCAGATGGGAAGTACATAGTGACAGGAGAGAATGGGCACCGGCCAGCTGTGCGCATC TGGGATGTGGAAGAGAAGACTCAGGTGGCAGAGATGCTGGGCCATAAGTATGGTGTGGCCTGTGTGGCCT TCTCACCCAATATGAAGCACATCGTGTCCATGGGCTACCAACATGACATGGTCCTCAATGTTTGGGACTG GAAGAAAGACATTGTGGTGGCTTCCAACAAGGTGTCGTGTCGAGTCATCGCCCTCTCCTTCTCTGAGGAC AGCAGCTATTTTGTCACTGTTGGCAATCGGCATGTTAGGTTTTGGTTCTTAGAAGCCTCTACAGAGGCCA AGGTAACCAGCACAATGCCCCTGGTAGGGCGCTCAGGCATCCTGGGTGAGCTGCACAACAATATCTTTTG TGGCGTGGCCTGCGGCCGGGGCCGGATGGCAGGCAATACCTTCTGTGTGTCCTACTCTGGCCTCCTCTGC CAGTTCAATGAGAAGAGGGTACTGGACAAGTGGATCAACCTAAAGGTCTCCTTGTCTTCCTGCCTCTGTG TCAGTGACGAGTTGATCTTCTGTGGATGCACAGACGGGATAGTCCGCATCTTCCAGGCCCACAGCCTGCT CTACCTCACGAACCTGCCCAAACCGCACTACCTGGGAATAGACGTGGCCCAGGGTCTGGACTCCAGCTTC CTTTTCCACAGAAAAGCAGAAGCAGTCTACCCAGATACAGTGGCCCTGACCTTTGACCCCATCCACCAGT GGCTGTCCTGTGTTTATAAAGACCACAGCGTCTACATCTGGGATGTCAAGGACATTAATGAAGTCAGCAA GATATGGTCAGAACTCTTCCACAGCTCCTTCGTCTGGAATGTAGAGGTGTACCCCGAATTCGAAGACCAG AGAGCTTGCCTGCCATCCGGGACTTTTCTGACTTGTTCCTCAGACAACACCATCCGCTTCTGGAATATGG ATAGCGGCTCTGACACTCAATGGCGGAAAAACATCTTCAGCGATTCCCTGCTGAAGGTGGTCTATGTGGA GAACGACATCCAGCACCTGCAGGACATGTCTCACTTCCCAGACCGGGGTAGTGAGAACGGGACGCCCATG GATATGAAAGCTGGGGTTCGAGTCATGCAGGTCAGTCCTGATGGCCAGCACTTGGCCTCAGGCGACCGCA GTGGAAATCTGAGGATCCACGAGCTGCACTTCATGGATGAGCTGATCAAGGTGGAGGCCCATGATGCTGA GGTGCTGTGCCTGGAGTACTCCAAGCCTGAGACAGGAGTGACTTTGCTGGCTTCAGCCAGTCGGGACCGG CTCATCCATGTGTTAAATGTAGAGAAGAATTACAACCTGGAGCAGACACTGGATGACCACTCCTCCTCCA TCACTGCCATTAAGTTTGCTGGCACCAGAGATGTCCAGATGATCAGCTGCGGAGCCGACAAGAGCATCTA CTTTCGCAGCGCCCAGCAGACCTCGGATGGGCTGCACTTTGTCCGTACCCACCACGTAGCAGAGAAGACC ACCTTGTATGACATGGATATTGACATCACACAAAAGTATGTGGCTGTGGCCTGCCAGGACCGCAATGTAA GGGTCTACAACACAGTGAGCGGGAAACAGAAGAAGTGCTACAAGGGCTCTCAGGGTGACGAAGGGTCCCT GCTGAAGGTCCATGTGGACCCCTCAGGCACCTTCCTGGCCACAAGCTGCTCTGACAAAAGCATCTCCGTA ATTGACTTTTACTCAGGCGAGTGTGTTGCCAAGATGTTTGGCCATTCAGAAATTGTCACTGGCATGAAGT TCACCTATGACTGCCGCCACTTGATCACAGTATCCGGAGACAGCTGTGTGTTCATCTGGCACCTGGGCCC CGAGATCACCACCTGCATGAAGCAGCACTTGATGGAGATCAACCACCAGGAGCAGCAGCATCTGCCCAAG GACCAGAAGTGGAGTGGCCCTCCCAGCCAGGAGACCTCTGCATCCACCCCAAGTGAGATCCGTTCCCTGA GCCCTGGGGAGCAGACAGAGGATGAGATGGAGGAGGAGTGTGAACCAGAAGAGACGCTGAAAACACCGTC CAAAGAGAGCTTGGACCCAGATCCTCGGTGCCTGCTGACCAATGGCAAGCTGCCACTGTGGGCAAAGCGG CTGCTAGGGGATGATGATGTGACAGACAGCTCAGCCTTCCACGCCAAGCGCAGCTACCAGCCACATGGCC GTTGGGCAGAGCGGGCTGAGCAGGAACCCCTCAAGACCATCCTGGATGCCTGGGCCCTGGATTCCTACTT TACACCCATGAAGCCTGGAAATCTTGAAGACTCTGTTCTGGACTCAGTAGAGCCACAAAACCTGGCAGGC CTGCTAAGTGAGTGCTCACCAGAGAATGGACACACGGCCCCGGGAGAAGGCCTGGTGAGCTACCTTTGTC ACTCAGAGTCAGGGAGCCCCAAAGAAGACAGCCGAGGACACCCCTCCTACCTGCCTCTACAGAGGGAAGG CACTGAGACAAGAGAACTCATCCTCTGCTCCCCAGAGGTGGAAGTGACAGTTACAGGGATGCACAGGGAG TATTATGAGGAGGCAGAGGCAGGACCTGAGGACCAGCAAGGCGATTCCTATCTCAGGGTCTCCTCCGTCA GTTCAAAGGATCAGAGCCCTCCTGAGGACTCAGGGGAATCAGAGGCTGAACTGGAGTGCAGCTTTGCTGC CGCCCATGTCTCAGCTCCTCGAACGGACCCTGGCCCTCACCTCACCATGACAGGTAAGCCAGAGCACTCA AGTACAGACGAGCTTGCCCAGCCTGGGGTGCCGGGCTTGGGCAATGGCTCCTTACCCCAGACGCCTGAGC AGGAGAAGTTCCTCCGCCACCATTTTGAGACTCTTACTGATGCCCCTGCTGAAGAGCTCTTTCCTGGATC ACTGGGAGACATAAAGATCTCAGAGACTGATGACTATTTCTTCAATCCCCGGCTGAGCATCTCCACACAG TTCCTCTCCCGCCTCCAGAAGACCTCCAGGTTCCCTCCCCGGCTGCCCCTGCACCTTATGAAGTCTCCAG AGGTTCAGCCTGTGGGCCAAGGGGGCAACCAGCCCAAAGCAGGGCCCCTGAGAGCAGGTACTGGCTACAT GTCCTCAGATGGGACCAATGTCCTCTCTGGGCAGATGGCTGAAGAGACTCCAGAGGCCTCGTGTCTACCA ACCCCTCCCATCACAGGCCTGGCATCCTGTGTCCCTCCTTCCTCAGTGATACCCACAGACAGGAAGCCTC CAACACCCACGTCTGGACTGACCACAGGCCGGGAGCAGAGTATTCCCGCCCCATCTCCGTGTTCTTACCT GGAGTCCACAACAAGCTCACATGCCAAGATGACTCGCAGCACCTCTCTCGGGGACAGTGAGGGCCCTGTG ACAGGCGAGCTACCCAGGCCACTCCACAAGCCCGTATCCCCCGGCCAGGAACCCCAGGCTGTTCCCACTA CAGTAGCACTCACTTCTAGTGTCAAAGGCCATGAGCCTGCACTGCCTGCTTGGGGCAACCATGAGGCCCG AGCCAGCCTGAGACTGACCTTATCCAGTGTCTGTGAGCAGCTCCTCTCTCCACCGCAACTGGAGCCACCC ACCACACCTGTGTGGTCTCAGGAAGCTGTGGATGTCCCACCTAGTATGGCAGTCACAGTAGCCAGCTTCT CAGCACCCAGCCCTGAAGACATGAGCACCCTGGGACTCCACAGTTCTGTGTTTCTTCCAAAGACCTCAGC CTCTGGGCCCCTCACCCCTCCTGCCCACCCCAACAGCCTCCAACTTCTAGAGACCAGGTCTAGGATGCCT GGCAGCACCCCTGCTCTCCTGGAGCCCACTCCTGATGCATCGAGTGGGAGCCCAGACAGCCCTGGACACT GTGGGGAGCCTGGGCGCCGAGCTGAAGTCCCACCCCCAGGACCCCTTGAGCTAGACAGTGTGGAATCAGT CTTGTACAGGCTGCAGACTGCCTTCCAAGAAGCCCTTGACCTTTACCGAATGTTGGCCTCCAGCAACCAG CTGGGTGCGGAGCAGCAGCAGGCACAGACTGAGCTGGCCTCCGCCTTCCACTGGATCCACCACCAGCTGG AAGCCAGCAACTGTGTGGGTGCAGCTAGCGTGGCCCCACCCCTCACACTGCCTAGCCCAGGCCCTCCCTC CCCACCTACACTTTGTCCCCTGGCCAGCCCAAACCTGCAGGCCCTGCTGGAACACTACTCGGAGCTGCTA GTGCAGGCAGTGAGGAGGAAGGTGAGGGGCGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217654 representing NM_001191623 Red=Cloning site Green=Tags(s) MMAALAAGGYARSDTIEKLSSVMAGVPARRNQSSPPPAPPLCLRRRTRLAAAPEDTVQNRVTLEKVLGIT AQNSSGLTCDPGTGHVAYLAGCVVVVLNPKENKQQHIFNTTRKSLSALAFSPDGKYIVTGENGHRPAVRI WDVEEKTQVAEMLGHKYGVACVAFSPNMKHIVSMGYQHDMVLNVWDWKKDIVVASNKVSCRVIALSFSED SSYFVTVGNRHVRFWFLEASTEAKVTSTMPLVGRSGILGELHNNIFCGVACGRGRMAGNTFCVSYSGLLC QFNEKRVLDKWINLKVSLSSCLCVSDELIFCGCTDGIVRIFQAHSLLYLTNLPKPHYLGIDVAQGLDSSF LFHRKAEAVYPDTVALTFDPIHQWLSCVYKDHSVYIWDVKDINEVSKIWSELFHSSFVWNVEVYPEFEDQ RACLPSGTFLTCSSDNTIRFWNMDSGSDTQWRKNIFSDSLLKVVYVENDIQHLQDMSHFPDRGSENGTPM DMKAGVRVMQVSPDGQHLASGDRSGNLRIHELHFMDELIKVEAHDAEVLCLEYSKPETGVTLLASASRDR LIHVLNVEKNYNLEQTLDDHSSSITAIKFAGTRDVQMISCGADKSIYFRSAQQTSDGLHFVRTHHVAEKT TLYDMDIDITQKYVAVACQDRNVRVYNTVSGKQKKCYKGSQGDEGSLLKVHVDPSGTFLATSCSDKSISV IDFYSGECVAKMFGHSEIVTGMKFTYDCRHLITVSGDSCVFIWHLGPEITTCMKQHLMEINHQEQQHLPK DQKWSGPPSQETSASTPSEIRSLSPGEQTEDEMEEECEPEETLKTPSKESLDPDPRCLLTNGKLPLWAKR LLGDDDVTDSSAFHAKRSYQPHGRWAERAEQEPLKTILDAWALDSYFTPMKPGNLEDSVLDSVEPQNLAG LLSECSPENGHTAPGEGLVSYLCHSESGSPKEDSRGHPSYLPLQREGTETRELILCSPEVEVTVTGMHRE YYEEAEAGPEDQQGDSYLRVSSVSSKDQSPPEDSGESEAELECSFAAAHVSAPRTDPGPHLTMTGKPEHS STDELAQPGVPGLGNGSLPQTPEQEKFLRHHFETLTDAPAEELFPGSLGDIKISETDDYFFNPRLSISTQ FLSRLQKTSRFPPRLPLHLMKSPEVQPVGQGGNQPKAGPLRAGTGYMSSDGTNVLSGQMAEETPEASCLP TPPITGLASCVPPSSVIPTDRKPPTPTSGLTTGREQSIPAPSPCSYLESTTSSHAKMTRSTSLGDSEGPV TGELPRPLHKPVSPGQEPQAVPTTVALTSSVKGHEPALPAWGNHEARASLRLTLSSVCEQLLSPPQLEPP TTPVWSQEAVDVPPSMAVTVASFSAPSPEDMSTLGLHSSVFLPKTSASGPLTPPAHPNSLQLLETRSRMP GSTPALLEPTPDASSGSPDSPGHCGEPGRRAEVPPPGPLELDSVESVLYRLQTAFQEALDLYRMLASSNQ LGAEQQQAQTELASAFHWIHHQLEASNCVGAASVAPPLTLPSPGPPSPPTLCPLASPNLQALLEHYSELL VQAVRRKVRGD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191623
ORF Size	4653 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191623.2, NP_001178552.2
RefSeq Size	4740 bp
RefSeq ORF	4656 bp
Locus ID	308492
MW	169.5 kDa

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