Iqsec2 (NM_001277425) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1562109
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217644 representing NM_001277425 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCGGGGTCCGGTCCTCCGGGTGGCCCGGGATCTGAGAGCCCCAACCGGGCGGTGGAGTACCTGC TGGAGCTGAACAACATCATCGAGAGTCAGCAGCAGCTGTTGGAGACCCAGCGGCGGCGCATAGAAGAGCT GGAGGGCCAGTTGGACCAGCTCACCCAGGAGAACCGCGACCTGCGGGAGGAGAGCCAGCTGCACCGTGGG GAACTGCACCGGGACCCCCTTGGCGCGCGGGATAGTCCTGGCCGCGAGAGCCAGTACCAGAACCTGCGTG AGACCCAGTTCCACCACCGCGAGCTGCGGGAGAGCCAGTTCCACCAGGCGTCCCGGGACGTGGGCTACCC GAACCGGGATGGCGCTTACCAGAACCGGGAAGCTATCTATCGGGATAAGGAGCGAGAAGCTTCCTATCAG CTCCAGGACACTACCGGTTACACAGCCCGCGAGCGCGACGTGGCCCAGTGCCACCTGCACCATGAGAACC CAGCCCTGGGTCGCGAGCGTGGCGGGAGGGAGGCTGGGCCAGCGCACCCGGGCCGCGAGAAGGAAGCCGG CTATTCCGCGGCGGTGGGCGTGGGGCAGCGGCCACCGCGGGAGCGGGGCCAGTTGAGCCGTGGCGCATCC AGAAGCTCCAGCCCTGGCGCGGGCGGAGGTCACAGCACCAGTACCAGCACCAGCCCTGCTACAACCCTCC AGAGAAAATCTGATGGTGAGAATTCCAGAACAGTCAGTGTGGAGGGTGATGCCCCAGGCAGTGACCTGAG CACAGCGGTTGATAGTCCTGGGAGCCAACCCCCCTACCGGCTGAGCCAACTGCCCCCAACCAGCAGCCAC ATGGGGGGCCCCCCTGCTGGCGTGGGCCTACCTTGGTCTCAGAGGGCTCGCCTCCAGCCAGCCAGTGTCG CCCTGAGGAAGCAGGAGGAGGAGGAGATAAAGCGCTCCAAGGCCCTGTCAGACAGCTATGAACTCTCCAC AGACCTACAGGACAAGAAGGTGGAAATGCTGGAGAGGAAGTATGGGGGCTCCTTCCTGAGCCGCAGGGCT GCCAGGACCATCCAGACAGCCTTTCGCCAGTATCGTATGAATAAGAACTTTGAGCGACTGCGCAGCTCGG CTTCAGAGAGCCGCATGTCCCGCCGCATCATCTTGTCCAACATGCGGATGCAGTTCTCCTTTGAGGAGTA CGAGAAAGCACAGAACCCTGCATACTTCGAAGGCAAGCCTGCCTCATTGGATGAGGGTGCAATGGCTGGA GCCCGGAGCCACCGGCTTGAACGGGGCCTCCCCTATGGAGGCTCCTGTGGTGGGGGCATCGATGGTGGTG GAAGTTCAGTCACCACATCTGGAGAGTTTTCTAATGACATCACAGAGCTCGAGGACTCCTTCTCCAAACA GGTAAAGTCTCTGGCTGAATCCATTGATGAGGCCTTGAACTGCCACCCATCAGGGCCTATGTCTGAGGAG CCAGGGTCAGCCCAACTGGAGAAGCGGGAATCAAAGGAACAGCAAGAAGACAGCTCAGCCACATCCTTCA GTGACCTCCCCCTCTACTTGGATGACCCAGTTCCTCCTCCATCACCTGAGCGACTGCCCAGCACAGAGCC CCCACCCCAAGGCCGCCCTGAGTTCTGGGCACCAGCTCCCCTCCCACCAGTTCCTCCACCAATGCCACCA GGGACCCGGGAAGATGGTAGCCGTGAGGAAGGCACTCGAAGGGGTCCTGGGTGCTTGGAGTGCCGGGATT TCCGGCTCCGGGCTGCACACCTCCCCCTCCTCACCATTGAGCCTCCCAGTGACAGTTCTGTAGACCTGAG TGACCGCTCAGATCGCGGCTCTGTCCACCGCCAGCTGGTGTATGAGGCTGATGGCTGCAGCCCCCATGGG ACCCTGAAGCACAAGGGGCCACCAGGCAGGGCCCCAATCCCACACCGCCACTACCCTGCCCCTGAGGGTC CAGCTCCAGCCCCACCAGGGCCCCTGCCACCAGCCCCCAATAGTGGTACTGGGCCCAGTGGTGTGGCTGG AGGTCGGAGGTTGGGAAAGTGTGAGGCAGCAGGTGAGAACTCTGATGGTGGAGACAATGAGAGCCTTGAG AGCTCCAGCAACTCTAATGAGACCATCAACTGCAGCTCTGGCTCCTCCTCTCGGGATAGCCTGAGGGAAC CTCCAGCCACTGGCCTGTGCAAGCAGACTTACCAGCGGGAGACAAGGCACAGCTGGGACTCGCCAGCCTT CAACAATGACGTGGTACAAAGAAGGCATTACAGAATTGGCCTCAACCTCTTCAATAAGAAGCCAGAGAAG GGTATCCAGTATTTGATCGAGCGAGGCTTCCTGTCAGACACTCCTGTGGGGGTGGCTCACTTCATCCTGG AGCGGAAAGGCCTGAGTCGGCAGATGATAGGGGAATTCCTGGGGAATCGACAGAAGCAGTTCAACAGAGA TGTGTTGGACTGTGTGGTGGATGAGATGGACTTCTCCTCTATGGATTTGGACGATGCACTCAGGAAGTTC CAATCCCATATCCGAGTTCAGGGTGAGGCCCAGAAAGTGGAGCGACTCATTGAAGCTTTCAGCCAGCGGT ACTGTGTCTGTAACCCTGCCCTCGTACGACAGTTCCGGAACCCAGACACTATCTTCATCCTTGCTTTTGC CATCATCCTCCTCAATACCGATATGTACAGCCCCAGTGTCAAGGCTGAACGCAAGATGAAGCTGGATGAC TTCATTAAGAACCTGAGAGGAGTTGACAATGGTGAAGACATCCCCCGAGACCTCCTAGTAGGCATCTACC AGCGCATCCAAGGTCGTGAACTAAGGACCAACGATGACCATGTGTCCCAGGTGCAGGCTGTGGAGCGCAT GATTGTTGGCAAGAAACCGGTCCTGTCGCTCCCTCACCGTCGACTGGTTTGCTGCTGCCAGCTCTATGAG GTGCCAGATCCAAATCGCCCCCAAAGGCTGGGGCTGCATCAGCGGGAGGTCTTCCTCTTCAATGACCTCC TTGTGGTCACCAAAATTTTCCAGAAGAAGAAGATCTTGGTGACATACAGCTTCCGTCAGTCCTTTCCCCT GGTGGAAATGCATATGCAGCTCTTCCAGAATTCATATTATCAGTTTGGAATCAAGTTACTATCTGCGGTA CCTGGAGGGGAACGAAAAGTTCTCATTATCTTTAATGCTCCTAGCCTCCAAGACAGGCTACGCTTTACCT CTGACCTGCGGGAGTCCATTGCTGAGGTGCAGGAGATGGAGAAGTACCGTGTGGAATCGGAGCTGGAGAA GCAGAAAGGTATGATGCGGCCTAATGCCTCACAACCTGGAGGAGCCAAGGACTCAGTGAATGGGACTCTG GCCCGCAGTAGTCTGGAGGACACTTATGGGGCAGGCGATGGGCTCAAGCGGGGTGCACTCAGTAGTTCCC TGCGAGACCTCTCTGATGCAGGGAAGCGGGGGCGGCGTAACAGCGTGGGATCGCTGGACAGCACCATCGA AGGGTCTGTTATTAGCAGTCCACGCCCTCACCAGAGGATGCCACCTCCGCCCCCACCCCCGCCGCCAGAG GAGTACAAGAGCCAGAGGCCAGTCTCCAACTCCTCATCTTTCCTGGGCTCCCTATTTGGAAGCAAGAGAG GCAAAGGGCCCTTCCAGATGCCACCACCGCCAACAGGCCAGGCCTCTGCCTCGTCTTCATCTGCTTCCTC CACCCACCACCACCATCACCACCACCACCATGGTCACAGCCATGGTGGCCTGGGGGTGCTACCTGATGGG CAGTCCAAGCTCCAGGCCCTGCATGCCCAGTATTGCCAAGGACCGGGCCCTGCCCCACCACCCTCTACAA CCCCCCAGCAACCCCCTCTGCCTCCACCCCCTCAGCAGCCTCCACCCCTGCCCCAGCTGGGCTCCATTCC ACCACCACCTGCCTCAGCCCCACCTGTGGGGCCACATCGACACTTCCATGCCCATGGCCCAGTTCCAGGA CCCCAGCATTATACCTTGGGCCGGCCAGGCAGAGCCCCAAGACGAGGGGCTGGAGGACACCCTCAGTTTG CTCCACATGGCCGCCACCCCTTGCACCAGCCCACATCCCCGTTACCCCTGTACAGTCCTGCTCCACAGCA CCCTCCTGCCCATAAACAGGGCCCCAAGCACTTCATCTTCAGTCACCACCCACAGATGATGCCAGCAGCA GGTGCAGCTGGGGGCCCTGGGTCCCGGCCACCAGGGGGATCCTACTCCCACCCTCACCACCCCCAATCAC CACTGTCCCCACACTCACCCATCCCACCTCACCCCTCCTACCCGCCCCTGCCCCCACCCTCACCTCACAC TCCACATTCACCCCTACCACCCACCTCCCCCCATGGCCCACTGCACGCCTCTGGGCCCCCTGGCACGGCT AATCCACCCAGTGCAAACCCCAAGGCCAAGCCAAGCCGGATCAGCACTGTGGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217644 representing NM_001277425 Red=Cloning site Green=Tags(s) MEAGSGPPGGPGSESPNRAVEYLLELNNIIESQQQLLETQRRRIEELEGQLDQLTQENRDLREESQLHRG ELHRDPLGARDSPGRESQYQNLRETQFHHRELRESQFHQASRDVGYPNRDGAYQNREAIYRDKEREASYQ LQDTTGYTARERDVAQCHLHHENPALGRERGGREAGPAHPGREKEAGYSAAVGVGQRPPRERGQLSRGAS RSSSPGAGGGHSTSTSTSPATTLQRKSDGENSRTVSVEGDAPGSDLSTAVDSPGSQPPYRLSQLPPTSSH MGGPPAGVGLPWSQRARLQPASVALRKQEEEEIKRSKALSDSYELSTDLQDKKVEMLERKYGGSFLSRRA ARTIQTAFRQYRMNKNFERLRSSASESRMSRRIILSNMRMQFSFEEYEKAQNPAYFEGKPASLDEGAMAG ARSHRLERGLPYGGSCGGGIDGGGSSVTTSGEFSNDITELEDSFSKQVKSLAESIDEALNCHPSGPMSEE PGSAQLEKRESKEQQEDSSATSFSDLPLYLDDPVPPPSPERLPSTEPPPQGRPEFWAPAPLPPVPPPMPP GTREDGSREEGTRRGPGCLECRDFRLRAAHLPLLTIEPPSDSSVDLSDRSDRGSVHRQLVYEADGCSPHG TLKHKGPPGRAPIPHRHYPAPEGPAPAPPGPLPPAPNSGTGPSGVAGGRRLGKCEAAGENSDGGDNESLE SSSNSNETINCSSGSSSRDSLREPPATGLCKQTYQRETRHSWDSPAFNNDVVQRRHYRIGLNLFNKKPEK GIQYLIERGFLSDTPVGVAHFILERKGLSRQMIGEFLGNRQKQFNRDVLDCVVDEMDFSSMDLDDALRKF QSHIRVQGEAQKVERLIEAFSQRYCVCNPALVRQFRNPDTIFILAFAIILLNTDMYSPSVKAERKMKLDD FIKNLRGVDNGEDIPRDLLVGIYQRIQGRELRTNDDHVSQVQAVERMIVGKKPVLSLPHRRLVCCCQLYE VPDPNRPQRLGLHQREVFLFNDLLVVTKIFQKKKILVTYSFRQSFPLVEMHMQLFQNSYYQFGIKLLSAV PGGERKVLIIFNAPSLQDRLRFTSDLRESIAEVQEMEKYRVESELEKQKGMMRPNASQPGGAKDSVNGTL ARSSLEDTYGAGDGLKRGALSSSLRDLSDAGKRGRRNSVGSLDSTIEGSVISSPRPHQRMPPPPPPPPPE EYKSQRPVSNSSSFLGSLFGSKRGKGPFQMPPPPTGQASASSSSASSTHHHHHHHHHGHSHGGLGVLPDG QSKLQALHAQYCQGPGPAPPPSTTPQQPPLPPPPQQPPPLPQLGSIPPPPASAPPVGPHRHFHAHGPVPG PQHYTLGRPGRAPRRGAGGHPQFAPHGRHPLHQPTSPLPLYSPAPQHPPAHKQGPKHFIFSHHPQMMPAA GAAGGPGSRPPGGSYSHPHHPQSPLSPHSPIPPHPSYPPLPPPSPHTPHSPLPPTSPHGPLHASGPPGTA NPPSANPKAKPSRISTVV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001277425
ORF Size	4464 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001277425.1, NP_001264354.1
RefSeq Size	5362 bp
RefSeq ORF	4467 bp
Locus ID	685244
MW	162.8 kDa

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