Pxdn (NM_001271261) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Perc64; Vpo1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217637 representing NM_001271261 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGTGCGCCCCACGCGCCGCTGCCTGCTGGCACTCGTGCTGTGCTTCGCCTGGTGGGCCATGGCGG TGGTCGCCTCGAAGCAAGGGGCAGGCTGTCCAAGCCGCTGCCTGTGCTTTCGTACCACCGTGCGCTGCAT GCATCTGTTGTTGGAGGCCGTGCCCGCGGTGGCGCCGCAGACCTCCATCCTAGATCTTCGGTTCAACAGA ATCAGAGAGATCCAACCCGGGGCATTCAGGAGGCTGAGGAACTTGAACACACTGCTTCTTAACAACAACC AGATCAAGAAGATTCCCAGTGGTGCCTTTGAGGACCTGGAGAACTTAAAATACCTCTATTTGTACAAGAA TGAGATCCAGTCAATTGACAGGCAAGCATTTAAGGGACTTGCCTCTCTAGAGCAACTGTACCTGCACTTT AATCAGATAGAGACGCTGGACCCAGAATCCTTCCAGCACCTGCCGAAGCTGGAGAGACTGTTTTTACATA ATAATCGCATCACACACTTAGTTCCTGGGACGTTCAGTCAGTTGGAGTCCATGAAACGGCTGCGATTAGA CTCAAATGCACTTCACTGTGACTGTGAAATATTATGGCTAGCGGATCTGCTGAAGACCTATGCCCAATCT GGAAATGCACAAGCAGCAGCCACCTGCGAGTATCCCAGACGCATCCAAGGACGCTCTGTGGCTACCATCA CCCCAGAAGAGCTGAACTGTGAAAGACCCCGGATCACCTCAGAGCCACAGGATGCAGATGTCACCTCAGG GAACACAGTGTACTTCACCTGCAGAGCTGAGGGCAACCCCAAACCTGAGATCATCTGGCTTCGAAACAAT AACGAGCTGAGCATGAAGACAGATTCTCGCTTAAACTTGCTGGATGACGGCACACTGATGATCCAGAACA CACAGGAGGCCGACGAGGGTGTCTACCAGTGCATGGCAAAAAACGTGGCTGGAGAGGCGAAAACGCATGA GGTGACCCTCAGGTACTTGGGATCCCCAGCCCGACCCACTTTTGTAATCCAGCCGCAGAACACAGAGGTA CTGGTGGGTGAGAGTGTCACTCTGGAGTGCAGTGCCACAGGTCACCCTCTGCCTCAGATCACGTGGACTC GAGGTGACCGGACACCCTTGCCGATTGACCCTCGAGTGAATATCACTCCCTCTGGAGGACTGTATATACA GAACGTCGCCCAGAGCGATAGTGGCGAGTACACGTGCTTTGCATCCAACAGCGTGGACAGCATCCATGCC ACGGCCTTCATCATTGTACAGGCCCTTCCTCAGTTCACTGTAACCCCTCAGAGCCGAGTGGTCATTGAAG GACAGACTGTGGACTTCCAGTGTGAGGCTAAGGGCTACCCTCAGCCAGTCATCGCCTGGACCAAGGGAGG GAGCCAGCTCTCAGTGGACAGGCGGCACCTGGTGCTGTCCTCAGGAACACTCAGGATTTCTGGGGTGGCC CTGCATGACCAGGGCCAGTATGAGTGCCAGGCCGTCAACATCATTGGCTCCCAGAAGGTGGTGGCCCACC TGACTGTACAGCCCAGAGTCACCCCAGTATTTGCCAGCATTCCCAGTGACATGACTGTAGAGGTGGGCAC CAACGTGCAGCTGCCTTGTAGCTCCCAGGGGGAACCAGAGCCAGCCATCACCTGGAACAAGGATGGTGTT CAGGTAACAGAAAGTGGAAAATTTCACATCAGCCCGGAAGGATTCTTGACCATCAATGATGTTGGCACTG CTGATGCAGGTCGCTATGAGTGTGTAGCTCGGAACACAATTGGATATGCCTCTGTGAGCATGGTACTCAG CGTGAACGTTCCTGATGTAAGTCGAAATGGGGATCCATACGTTGCTACCTCTATTGTTGAAGCCATTGCA ACTGTTGACAGAGCCATCAACTCTACAAGGACACACTTGTTTGACAGCCGACCTCGTTCTCCAAATGACC TACTTGCTCTGTTCCGGTACCCACGGGATCCGTACACCGTGGGACAAGCCAGGGCAGGAGAGATATTCGA GCGGACCCTGCAGCTGATCCAGGAACATGTTCAGCATGGCTTGATGGTGGACTTGAATGGAACAAGTTAC CACTACAATGATCTGGTGTCCCCGCAGTACCTGAGCCTCATCGCCAACCTGTCAGGCTGCACTGCACACC GTCGTGTGAACAACTGCTCAGACATGTGCTTCCACCAGAAGTATAGGACGCACGATGGCACGTGCAACAA TCTGCAGCACCCGATGTGGGGAGCCTCACTGACCGCCTTCGAGCGCCTGCTGAAGGCTGTGTATGAGAAT GGATTCAACACGCCCCGTGGCATTAATTCCCAGCGTCAGTACAATGGGCATGTACTACCCATGCCCCGCC TGGTGTCCACCACACTGATCGGGACAGAGGTGATCACCCCAGATGAGCAGTTTACACACATGCTGATGCA ATGGGGCCAGTTCCTTGACCATGACCTAGACTCTACAGTGGTGGCCCTGAGCCAGGCCCGCTTCTCTGAT GGGCAGCATTGCAGCTCAGTGTGCAGCAATGACCCTCCCTGTTTCTCGGTCATGATCCCCCCCAACGATC CCAGAGTGCGGAGTGGGGCCCGATGCATGTTCTTCGTGCGGTCAAGCCCTGTGTGCGGCAGTGGCATGAC ATCCCTGCTCATGAACTCTGTGTACCCTCGAGAGCAGATCAACCAGCTCACTTCCTACATTGACGCATCC AATGTGTATGGCAGCACAGACCACGAAGCCCGGAGCATCCGGGACCTGGCCAGTCATCGTGGCCTGCTAC GTCAGGGTATCGTGCAGAGGTCTGGCAAGCCCCTGCTTCCCTTTGCCACGGGGCCACCCACTGAGTGTAT GCGTGATGAGAATGAGAGTCCAATACCGTGCTTTCTGGCCGGTGACCATCGTGCCAACGAGCAGCTGGGC CTGACCAGCATGCATACGCTGTGGTTCCGGGAGCACAACCGCATCGCAGCAGAGCTGTTGAAGCTGAACC CGCACTGGGATGGGGACACTGTCTACCATGAGACCCGCAAGATAGTGGGGGCAGAGATCCAGCACATTAC CTACCGGCACTGGCTACCCAAGATCCTGGGGGAGGTGGGCATGAAGATGCTAGGTGAGTACCGGGGCTAC GACCCCAGCGTCAATGCTGGCATCTTTAATGCCTTTGCCACCGCAGCCTTCAGGTTCGGTCACACTCTGA TCAACCCTCTGCTCTACCGGCTGGATGAGAACTTTGAGCCCATCCCACAGGGCCACGTGCCCCTCCACAA AGCCTTCTTCTCACCCTTCCGGATTGTCAATGAGGGGGGCATCGACCCACTTCTCCGAGGGCTGTTTGGA GTGGCAGGGAAGATGCGCATTCCCTCTCAACTGCTGAACACTGAGCTCACAGAGAGGCTTTTCTCCATGG CACACACAGTGGCCCTTGACCTGGCTGCCATCAATATCCAGCGAGGCCGGGACCATGGCATCCCACCCTA CCATGACTACAGGGTCTACTGCAACCTGTCAGCCGCTTATACCTTTGAGGACCTGAAAAATGAGATCAAG AGTCCCGTGATCCGGGAGAAACTGCAGAGGCTATATGGCTCGACACTCAACATCGATCTGTTCCCGGCCC TCATGGTGGAAGACCTGGTCCCCGGCAGCCGCTTGGGGCCCACACTCATGTGCCTGCTCAGCACACAGTT CCGACGCCTGCGGGATGGAGACAGGTTGTGGTATGAGAACCCAGGCGTGTTCTCCCCTGCCCAGCTGACT CAGCTCAAGCAGACATCCCTGGCGAGGATCCTTTGTGACAACTCAGACAACATCACCCGCGTGCAGCACG ATGTGTTCAGGGTGGCAGAGTTCCCACACGGTTACAGCAGCTGCGAGGACATCCCCAGAATGGACCTGCG AGTGTGGCAGGACTGTTGTGAAGACTGTAGGACCAGGGGACAATTCAATGCCTTCTCCTACCATTTCCGG GGAAGAAGGTCTCTAGAATTCAGCTATGAGGAGGATAAGCCCACAAAGAGAGCCAGGTGGCGGAAAGCAC TAAGTGTAAAACATGGGAAACATCATAGCAATGCCACATCAGTCACCCATGAGCACTTGGAAGGGCCAGC AACTAATGATCTCAAGGAGTTTGTTCTAGAAATGCAAAAGATCATCACAGACCTCAGAAAACAGATAAAC AGCCTGGAGTCTCGGCTCAGCACCACAGAGTGTGTGGATGACAGTGGTGAATCCCACGGCAGCAACACGA AGTGGGAAAAAGACACATGCACAGTTTGCGAGTGCAAAAATGGCCAGGTCACCTGCTTTGTGGAAGCTTG CCAGCCTGCAGCCTGCCCGCAGCCTGTGAAAGTCGAAGGCGCTTGCTGTCCTGTCTGCTTAAGGAACACT GAAGAGGAAAAGCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217637 representing NM_001271261 Red=Cloning site Green=Tags(s) MAVRPTRRCLLALVLCFAWWAMAVVASKQGAGCPSRCLCFRTTVRCMHLLLEAVPAVAPQTSILDLRFNR IREIQPGAFRRLRNLNTLLLNNNQIKKIPSGAFEDLENLKYLYLYKNEIQSIDRQAFKGLASLEQLYLHF NQIETLDPESFQHLPKLERLFLHNNRITHLVPGTFSQLESMKRLRLDSNALHCDCEILWLADLLKTYAQS GNAQAAATCEYPRRIQGRSVATITPEELNCERPRITSEPQDADVTSGNTVYFTCRAEGNPKPEIIWLRNN NELSMKTDSRLNLLDDGTLMIQNTQEADEGVYQCMAKNVAGEAKTHEVTLRYLGSPARPTFVIQPQNTEV LVGESVTLECSATGHPLPQITWTRGDRTPLPIDPRVNITPSGGLYIQNVAQSDSGEYTCFASNSVDSIHA TAFIIVQALPQFTVTPQSRVVIEGQTVDFQCEAKGYPQPVIAWTKGGSQLSVDRRHLVLSSGTLRISGVA LHDQGQYECQAVNIIGSQKVVAHLTVQPRVTPVFASIPSDMTVEVGTNVQLPCSSQGEPEPAITWNKDGV QVTESGKFHISPEGFLTINDVGTADAGRYECVARNTIGYASVSMVLSVNVPDVSRNGDPYVATSIVEAIA TVDRAINSTRTHLFDSRPRSPNDLLALFRYPRDPYTVGQARAGEIFERTLQLIQEHVQHGLMVDLNGTSY HYNDLVSPQYLSLIANLSGCTAHRRVNNCSDMCFHQKYRTHDGTCNNLQHPMWGASLTAFERLLKAVYEN GFNTPRGINSQRQYNGHVLPMPRLVSTTLIGTEVITPDEQFTHMLMQWGQFLDHDLDSTVVALSQARFSD GQHCSSVCSNDPPCFSVMIPPNDPRVRSGARCMFFVRSSPVCGSGMTSLLMNSVYPREQINQLTSYIDAS NVYGSTDHEARSIRDLASHRGLLRQGIVQRSGKPLLPFATGPPTECMRDENESPIPCFLAGDHRANEQLG LTSMHTLWFREHNRIAAELLKLNPHWDGDTVYHETRKIVGAEIQHITYRHWLPKILGEVGMKMLGEYRGY DPSVNAGIFNAFATAAFRFGHTLINPLLYRLDENFEPIPQGHVPLHKAFFSPFRIVNEGGIDPLLRGLFG VAGKMRIPSQLLNTELTERLFSMAHTVALDLAAINIQRGRDHGIPPYHDYRVYCNLSAAYTFEDLKNEIK SPVIREKLQRLYGSTLNIDLFPALMVEDLVPGSRLGPTLMCLLSTQFRRLRDGDRLWYENPGVFSPAQLT QLKQTSLARILCDNSDNITRVQHDVFRVAEFPHGYSSCEDIPRMDLRVWQDCCEDCRTRGQFNAFSYHFR GRRSLEFSYEEDKPTKRARWRKALSVKHGKHHSNATSVTHEHLEGPATNDLKEFVLEMQKIITDLRKQIN SLESRLSTTECVDDSGESHGSNTKWEKDTCTVCECKNGQVTCFVEACQPAACPQPVKVEGACCPVCLRNT EEEKP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271261
ORF Size	4425 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271261.1, NP_001258190.1
RefSeq Size	6563 bp
RefSeq ORF	4428 bp
Locus ID	554172
MW	165.4 kDa

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