Ptpru (NM_001191575) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217627 representing NM_001191575 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCGAGCTCAGGCTCTGGTCCTGGCGCTCACCTTCCAGTTCTGCGCGCCCGAGACCGAGACTCCCG CAGCTGGCTGCACCTTCGAGGAGGCGAGTGACCCGGTTGTGCCCTGCGAGTTCAGCCAGGCTCAGTATGA TGACTTCCAGTGGGAGCAAGTACGGATCCACCCTGGCACCCGGACCCCTGCAGACCTGCCCCATGGTGCC TACTTGATGGTTAATGCTTCCCAGCATGCTCCAGGCCAGAGGGCCCATATCATCTTCCAGACCCTGAGCG AGAACGACACCCACTGTGTGCAGTTCAGCTACTTCCTGTTTAGCCGGGATGGGCACAGCCCAGGCACCCT GGGCGTCTATGTGCGTGTGAACGGGGGCCCTCTGGGCAGTGCCGTGTGGAATATGACTGGATCCCACGGC CGTCAGTGGCATCAGGCTGAACTGGCTGTCAGCACCTTCTGGCCCAATGAGTATCAGGTGCTGTTTGAGG CTCTCATCTCCCCAGACCATAAGGGCTACATAGGCTTAGATGACATCTTGCTATTCAGCCATCCCTGCGC GAAGGCCCCTCACTTCTCCCGCCTTGGAGACGTGGAGGTCAATGCGGGCCAGAATGCATCCTTCCAATGC ATGGCAGCAGGCAGAGCTGCAGAGGCAGAGCGTTTCTTCCTGCAGCGGCAGAGTGGGGTGCTAGTGCCTG CGGCTGGGGTGCGGCACATCAGTCACCGTCGCTTCCTGGCCACTTTTCCACTGGCCTCTGTAGGCCGCTC GGAGCAGGATCTGTACCGTTGCGTGTCCCAGGCCCCGCGCGGTGCTGGAGTCTCCAACTTCGCAGAGCTC ATCGTCAAAGAGCCTCCCACCCCCATCGCGCCCCCACAGCTGCTGCGTGCAGGCCCCACCTACCTCATCA TCCAGCTCAACACCAACTCCATCATTGGCGACGGGCCGATCGTGCGCAAGGAGATCGAGTACCGAATGGC ACGGGGCCCGTGGGCCGAGGTTCACGCTGTCAACCTGCAGACCTACAAGCTGTGGCATCTAGACCCGGAC ACTGAGTATGAGATCAGCGTGCTGCTTACCCGCCCGGGAGATGGAGGCACAGGCCGCCCAGGGCCACCAC TGCTCAGCCGGACCAAGTGCGCAGAGCCCACGAGGGCCCCCAAGGGCCTGGCTTTTGCTGAGATCCAGGC TCGTCAGCTGACCCTGCAGTGGGAGCCCCTGGGCTACAATGTCACGCGTTGTCATACCTATGCTGTGTCC CTTTGTTATCACTACACCCTGGGCGGCAGCCACAACCAGACCATCCGGGAGTGTGTGAAGATGGAGCGGG GCGCCAGCCGCTACACCATCAAGAATCTGCTGCCGTTCAGGAACGTCCACGTGCGTCTGATTCTCACAAA CCCTGAGGGGCGCAAGGAGGGCAAGGAGGTCACCTTCCAGACAGATGAAGATGTGCCTGGTGGGATCGCC GCTGAGTCTCTCACCTTCACCCCACTAGAGGACATGATCTTTCTCAAGTGGGAGGAGCCCCAGGAACCCA ATGGCCTCATCACTCAGTATGAGATCAGCTACCAAAGCATTGAGTCCTCAGACCCAGCGGTGAACGTGCC CGGCCCGAGACGCACCATCTCCAAACTCCGAAATGAGACTTACCATGTCTTCTCCAACCTGCAACCCGGC ACCACATACCTGTTCTCTGTGCGTGCTCGTACAAGCAAGGGCTTTGGCCAGGCGGCTCTCACCGAGATAA CCACCAACATCTCAGCACCCAGCTTTGATTATGCCGACATGCCGTCACCCCTGGGCGAGTCTGAGAACAC CATCACTGTGCTGTTGAGGCCGGCCCAGGGCCGAGGAGCACCCATCAGCGTCTACCAGGTGGTTGTGGAG GAAGAGCGGCCACGGCGCTTGCGGCGCGAGCCTGGAGGTCAGGACTGCTTCTCGGTACCTCTGACCTTTG AGACGGCCCTGGCTCGCGGCCTGGTGCACTACTTTGGGGCTGAACTGGCTGCCAGCAGCCTGCTTGAGGC CATGCCCTTCACTGTGGGTGACAACCAGACCTATCGTGGCTTCTGGAACCCACCGCTTGAGCCCAGGAAG GCCTACCTCATCTATTTCCAGGCAGCGAGCCACCTGAAAGGGGAAACCCGACTGAACTGCATCCGAATTG CCAGGAAAGCTGCGTGCAAGGAGAGCAAGCGACCCCTCGAAGTGTCCCAGAGGTCGGAGGAGATGGGGCT CATCCTGGGTATCTGTGCAGGTGGCCTTGCCGTCCTCATTCTCCTCCTGGGCGCCATCATTATCATCATC CGTAAAGGGAGGGACCGCTATGCCTACTCTTACTACCCGAAGCCAGTGAACATGGCAAAAGCCACGGTCA ACTATCGCCAGGAGAAGACCCACATGATGACTGCTGTGGACCGTAGCTTCACAGATCAGAGCACTCTGCA GGAGGATGAGCGGCTGGGCCTGTCCTTCATGGATGCTCCTGGCTACAGCCCTCGGGGAGACCAGAGAAGC AGTGGGGTCACTGAGGCCAGTAGCCTCCTGGGGGGCTCTCCTCGGCGCCCCTGTGGCCGGAAGGGCTCCC CGTATCACACAGGGCAGCTTCACCCTGCAGTACGAGTGGCTGACCTGCTGCAGCACATCAACCAGATGAA GACAGCCGAGGGCTACGGCTTCAAGCAAGAGTACGAGAGTTTCTTTGAAGGCTGGGATGCCACCAAGAAG AAAGACAAGCTGAAGAGCGGCCGGCAGGAGCCAGTGTCTGCCTATGACCGACACCGAGTGAAGCTGCACC CGATGCTGGGAGACCCTGATGCCGACTACATCTCTGCCAACTACATAGACGGCTACCACAGGTCAAACCA CTTCATAGCCACTCAAGGGCCAAAGCCTGAGATGATCTACGACTTCTGGCGCATGGTGTGGCAGGAGCAG TGTGTGAGCGTCGTCATGATCAGCAAGCTGGTAGAGGTGGGCAGGGTAAAGTGCTCTCGATACTGGCCCG AGGACTCAGACATGTATGGGGACATCAAGATCACGCTGGTAAAGACAGAGACACTGGCTGAGTACGTGGT GCGCACCTTTGCCCTGGAGCGGAGAGGTTACTCAGCCCGGCATGAGGTCCGCCAGTTCCACTTCACAGCG TGGCCAGAGCATGGTGTTCCCTACCATGCCACGGGGCTGCTGGCCTTCATCCGGCGCGTGAAGGCTTCCA CTCCACCTGATGCTGGGCCTGTTGTCATTCACTGCAGTGCAGGCACTGGCCGCACAGGCTGCTACATCGT CTTGGATGTGATGCTGGACATGGCTGAGTGTGAGGGGGTCGTGGACATTTACAACTGCGTGAAGACCCTC TGTTCCCGGCGGGTCAACATGATCCAGACCGAGGAACAATACATCTTCATCCACGATGCGATTCTGGAGG CCTGCCTGTGTGGGGAGACCACCATCCCTGTCAATGAGTTCAAGGCCACCTATAGGGAGATGATCCGCAT TGACCCTCAGAGTAACTCCTCCCAGCTTCGGGAAGAGTTCCAGACACTGAACTCGGTCACACCGCCGCTG GACGTGGAGGAGTGTAGCATTGCCCTGCTGCCCCGGAATCGAGACAAGAACCGTAGCATGGACGTGCTGC CGCCAGACCGCTGCCTGCCCTTCCTCATCTCCAGTGATGGGGACCCCAACAACTACATCAATGCAGCCCT AACTGATAGCTACACACGGAGCGCCGCCTTCATCGTGACCCTGCACCCACTGCAGAGTACCACACCCGAC TTCTGGCGGCTGGTCTACGACTATGGGTGCACCTCCATTGTGATGCTCAACCAGCTTAACCAGTCCAACT CCGCCTGGCCCTGCCTGCAGTACTGGCCAGAACCAGGCAGACAGCAGTATGGCCTCATGGAGGTGGAGTT TGTGTCTGGCACAGCAAACGAGGATCTGGTGTCCCGAGTGTTCCGGGTGCAGAACTCTTCAAGGCTGCAG GAGGGCCACCTGCTGGTTCGTCACTTTCAGTTCCTGCGTTGGTCTGCTTATCGGGACACGCCTGACTCCA GGAAGGCCTTCCTGCACCTGTTGGCTGAGGTGGACAAGTGGCAGGCGGAGAGTGGAGATGGGCGCACCGT GGTGCATTGTCTCAATGGCGGTGGCCGCAGTGGCACCTTCTGCGCCTGTGCAACGGTCTTGGAAATGATC CGCTGTCACAGCCTGGTGGATGTTTTCTTTGCTGCCAAAACGCTTCGGAACTACAAGCCCAATATGGTGG AGACCATGGATCAATACCATTTCTGCTACGATGTGGCCCTGGAGTACCTGGAGGCTCTGGAGTTGAGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217627 representing NM_001191575 Red=Cloning site Green=Tags(s) MARAQALVLALTFQFCAPETETPAAGCTFEEASDPVVPCEFSQAQYDDFQWEQVRIHPGTRTPADLPHGA YLMVNASQHAPGQRAHIIFQTLSENDTHCVQFSYFLFSRDGHSPGTLGVYVRVNGGPLGSAVWNMTGSHG RQWHQAELAVSTFWPNEYQVLFEALISPDHKGYIGLDDILLFSHPCAKAPHFSRLGDVEVNAGQNASFQC MAAGRAAEAERFFLQRQSGVLVPAAGVRHISHRRFLATFPLASVGRSEQDLYRCVSQAPRGAGVSNFAEL IVKEPPTPIAPPQLLRAGPTYLIIQLNTNSIIGDGPIVRKEIEYRMARGPWAEVHAVNLQTYKLWHLDPD TEYEISVLLTRPGDGGTGRPGPPLLSRTKCAEPTRAPKGLAFAEIQARQLTLQWEPLGYNVTRCHTYAVS LCYHYTLGGSHNQTIRECVKMERGASRYTIKNLLPFRNVHVRLILTNPEGRKEGKEVTFQTDEDVPGGIA AESLTFTPLEDMIFLKWEEPQEPNGLITQYEISYQSIESSDPAVNVPGPRRTISKLRNETYHVFSNLQPG TTYLFSVRARTSKGFGQAALTEITTNISAPSFDYADMPSPLGESENTITVLLRPAQGRGAPISVYQVVVE EERPRRLRREPGGQDCFSVPLTFETALARGLVHYFGAELAASSLLEAMPFTVGDNQTYRGFWNPPLEPRK AYLIYFQAASHLKGETRLNCIRIARKAACKESKRPLEVSQRSEEMGLILGICAGGLAVLILLLGAIIIII RKGRDRYAYSYYPKPVNMAKATVNYRQEKTHMMTAVDRSFTDQSTLQEDERLGLSFMDAPGYSPRGDQRS SGVTEASSLLGGSPRRPCGRKGSPYHTGQLHPAVRVADLLQHINQMKTAEGYGFKQEYESFFEGWDATKK KDKLKSGRQEPVSAYDRHRVKLHPMLGDPDADYISANYIDGYHRSNHFIATQGPKPEMIYDFWRMVWQEQ CVSVVMISKLVEVGRVKCSRYWPEDSDMYGDIKITLVKTETLAEYVVRTFALERRGYSARHEVRQFHFTA WPEHGVPYHATGLLAFIRRVKASTPPDAGPVVIHCSAGTGRTGCYIVLDVMLDMAECEGVVDIYNCVKTL CSRRVNMIQTEEQYIFIHDAILEACLCGETTIPVNEFKATYREMIRIDPQSNSSQLREEFQTLNSVTPPL DVEECSIALLPRNRDKNRSMDVLPPDRCLPFLISSDGDPNNYINAALTDSYTRSAAFIVTLHPLQSTTPD FWRLVYDYGCTSIVMLNQLNQSNSAWPCLQYWPEPGRQQYGLMEVEFVSGTANEDLVSRVFRVQNSSRLQ EGHLLVRHFQFLRWSAYRDTPDSRKAFLHLLAEVDKWQAESGDGRTVVHCLNGGGRSGTFCACATVLEMI RCHSLVDVFFAAKTLRNYKPNMVETMDQYHFCYDVALEYLEALELR SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191575
ORF Size	4338 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191575.1, NP_001178504.1
RefSeq Size	5129 bp
RefSeq ORF	4341 bp
Locus ID	116680
MW	162.4 kDa
Gene Summary	transmembrane receptor type protein tyrosine phosphatase that is expressed in neuroepithelium; may play a role in neurogenesis [RGD, Feb 2006]

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