Nsd2 (NM_001191552) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1565590; Whsc1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217596 representing NM_001191552 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCAGGCACCAGAAATCCTTGGCAGTGCAAATGGAAAGACTCAGAACTGTGAAGTGAATCACGAAT GTTCTGTATTCCTCAGCAAAGCTCAGCTTTCTAACAGCCTACAGGAGGGGGTCATGCAGAAATTTAACGG GCATGATGCACTCCCCTTTCTTCCAGCAGAGAAGTTGAAAGATCTTACTTCTTGTGTGTTTAATGGAGAA CCTGGTGCTCATGATACTAAGTTGTGTTTTGAGACCCAGGAAGTAAAAGGAATTGGGACACCACCCAATA CTACTCCTATCAAAAATGGCTCTCCAGAAATTAAACTGAAAATCACCAAAACATACATGAATGGGAAACC TCTCTTTGAATCTTCAATTTGTGGTGATGGCGCTGCTGATATGTCTCAGTCAGAAGAAAATGGACAAAAA TCGGATAATAAGACTAGGAGGAACAGGAAGAGGAGCATAAAATATGACTCTTTGCTGGAGCAGGGCCTTG TGGAAGCAGCTTTAGTGTCCAAGATCTCAAGTCCTGAAGATAAAAAGATTCCAGTTAAGAAGGAGTCCTG TCCAAATAGTGGCAGAGACAGAGACCTCTTGTTAAAATACAACGTTGGTGATTTGGTGTGGTCCAAAGTG TCAGGTTACCCTTGGTGGCCTTGCATGGTTTCTGCTGATCCACTCCTTCACAATCATACCAAACTTAAAG GTCAGAAAAAAAGTGCACGCCAGTATCATGTACAATTCTTTGGTGATGCCCCAGAAAGAGCTTGGATATT TGAGAAGAGCCTTGTTGCTTTTGAAGGAGAAGAACAATTTGAAAAATTATGCCAAGAAAGTGCCAAGCAG GCACCCACGAAAGCTGAGAAAATCAAGTTGTTGAAACCTATTTCAGGGAGATTGAGAGCCCAGTGGGAAA TGGGCATTGTCCAGGCTGAGGAAGCTGCTAGCATGTCAGTAGAGGAGCGGAAAGCCAAGTTTACTTTCCT TTATGTGGGGGACCAACTGCGCCTCAACCCTCAAGTGGCTAAGGAGGCTGGCATTGCTACAGAACCTTTG GGAGAAATGGTGGACTCTTCAGTGGCCAATGAAGAAGCTGCTGTAGACCCTGGGACTATGAGAGAAGAGG ACATTCCCGTAAAGAGAAGGCGAAGAGCCAAAAGGTCTAGTTCTGCTGAGAACCAGGAAGGAGACCCCGG CACAGAAAAGAGTACACCTCCAAAGATGGCGGATGCTGAACCTAAGAGAGGCGTAGGTTCTCCTGCCGGG AGGAAAAGGTCCACAGGATCTGCCTCACGGAGCAGAAAGGGAGACTCAGCAGCCCAGTTCTTAGTCTTTT GTCAAAAACACAGGGATGAGGTCGTAGCTGAACACCCAGATGCCTCAGAGGAGGAGATTGAAGAATTGCT TGGGTCCCAATGGAGCATGCTCAATGAAAAGCAGAAAGCACGATATAATACAAAGTTTTCCCTAATGATC TCTGCCCAGTCTGAAGAAGACTCTGGAAATACCAGTGGGAAAAAAAGGACCCACACAAAAAGGACAGATG ACCCTCCTGAGGATGTGGATGTAGAAGACGCACCCAGGAAAAGACTTAGGACAGATAAGCACAGTCTTCG GAAGCAAAGAGAGACAATCACCGACAAAACGGCCAGAACGAGCTCGTACAAGGCTATAGAGGCAGCCTCC TCACTCAAGAGCCAGGCAGCAACGAAAAATTTGTCTGATGCTTGCAAACCACTGAAGAAGCGAAATCGGG CTTCTGCAACAGCGTCTTCAGCACTTGGGTTTAACAAAAGTTCATCTCCTTCTGCATCCTTAACTGAGAA TGAGGTCTCAGACAACCCAGGAGATGAACCGTCAGAGTCTCCGTATGAAAGTGCAGATGAAACACAGACT GAAGCGTCCGTCTCATCTAAGAAATCTGAAAGAGGAATGGCTGCCAAAAAGGAGTACGTGTGTCAGCTGT GTGAGAAGACAGGCAGTCTCTTACTGTGTGAAGGCCCCTGTTGTGGAGCATTCCACTTAGCCTGCCTTGG ACTTTCCCAGAGACCAGAAGGAAGATTCACCTGCACTGAATGTGCCTCAGGGATTCACTCATGCTTCGTA TGTAAAGAAAGCAAGATGGAGGTGAAACGCTGTATGGTAAACCAATGTGGAAAGTTTTACCATGAAGCCT GTGTGAAAAAATACCCTCTCACTGTGTTTGAGAGCCGAGGCTTTCGCTGTCCTCTCCACAGCTGTATGAG CTGCCATGCCTCAAACCCTTCAAATCCGCGGCCATCAAAAGGTAAAATGATGCGGTGCGTCCGATGCCCT GTCGCCTATCATGGCGGGGATGCCTGCCTGGCAGCGGGATGTTCCGTGATTGCTTCTAACAGCATAATCT GCACAGGCCACTTCACTGCCCGCAAGGGGAAGCGGCACCACACTCATGTCAATGTGAGCTGGTGCTTCGT GTGCTCCAAAGGGGGAAGCCTTCTGTGCTGCGAGGCCTGTCCGGCAGCCTTCCACCCTGATTGTTTGAGC ATCGAGATGCCTGATGGCAGCTGGTTCTGCAATGACTGCCGAGCTGGGAAGAAGCTCCACTTTCAAGACA TCATTTGGGTCAAACTTGGGAACTACAGATGGTGGCCAGCAGAAGTTTGCCATCCCAAAAATGTTCCCCC AAATATTCAGAAAATGAAGCACGAGATTGGAGAATTCCCTGTGTTTTTCTTCGGGTCTAAAGATTATTAC TGGACGCATCAGGCACGAGTGTTCCCATACATGGAGGGGGACCGGGGCAGCCGCTACCAGGGGGTCAGAG GGATTGGAAGAGTCTTCAAGAACGCATTGCAAGAAGCCGAAGCTCGTTTTAATGAAATCAAACTTCAGCG GGAAGCCCGAGAAACACAGGAGAGTGAGAGAAAGCCTCCACCATACAAGCATATCAAGGTGAATAAACCT TATGGTAAAGTCCAGATCTATACAGCTGACATTTCTGAAATCCCAAAATGCAACTGTAAGCCCACGGATG AGAACCCCTGTGGCTCTGATTCAGAGTGTCTAAACAGGATGCTAATGTTTGAGTGCCACCCGCAGGTGTG CCCTGCAGGGGAATATTGCCAGAACCAGTGTTTCACTAAGCGCCAGTACCCGGAGACCAAGATCATCAAG ACCGATGGCAAAGGGTGGGGCCTGGTTGCCAAAAGGGACATCAGAAAGGGAGAATTTGTTAATGAGTATG TTGGCGAACTGATTGATGAAGAGGAGTGTATGGCAAGAATCAAATATGCACATGAGAATGACATCACTCA CTTTTACATGCTCACCATAGACAAGGACCGCATAATTGATGCTGGCCCCAAAGGGAATTACTCGCGGTTC ATGAACCATAGCTGCCAGCCCAACTGTGAGACCCTGAAGTGGACAGTGAATGGGGACACACGGGTTGGCC TGTTTGCTGTGTGCGACATCCCTGCAGGTACAGAGCTGACTTTCAACTATAACCTCGATTGTTTGGGCAA TGAAAAGACAGTCTGTCGGTGTGGAGCCTCCAACTGTAGTGGGTTCCTTGGAGACAGACCAAAGACATCA ACATCCCTTTCTTCAGAGGAAAAGAGTAAAAAGGCCAAGAAGAAAACCAGGAGGCGCAGAGCCAAGGGTG AAGGAAAGCGGCAGTCAGAGGACGAATGCTTCCGCTGTGGTGATGGCGGACAACTGGTGCTGTGTGACCG CAAGTTCTGCACCAAGGCCTACCACCTATCCTGCTTGGGCTTGGGCAAGCGGCCCTTTGGGAAGTGGGAA TGTCCTTGGCATCACTGTGATGTGTGTGGCAAACCTTCTACCTCATTTTGCCACCTCTGCCCCAACTCAT TCTGTAAGGAACACCAAGATGGGACTGCTTTCCGCTCCACCCAGGATGGGCAATCCTACTGCTGTGAGCA TGACCTGAGGGCAGACTCTGCAAATAATACCAAGACTGAGAAACCCTTTCTAGACTCACTCAAGGCGAAG GGGAAGAGGAAGAAAAGGCGGTGCTGGCGAAGGGTCACAGACGGCAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217596 representing NM_001191552 Red=Cloning site Green=Tags(s) MKQAPEILGSANGKTQNCEVNHECSVFLSKAQLSNSLQEGVMQKFNGHDALPFLPAEKLKDLTSCVFNGE PGAHDTKLCFETQEVKGIGTPPNTTPIKNGSPEIKLKITKTYMNGKPLFESSICGDGAADMSQSEENGQK SDNKTRRNRKRSIKYDSLLEQGLVEAALVSKISSPEDKKIPVKKESCPNSGRDRDLLLKYNVGDLVWSKV SGYPWWPCMVSADPLLHNHTKLKGQKKSARQYHVQFFGDAPERAWIFEKSLVAFEGEEQFEKLCQESAKQ APTKAEKIKLLKPISGRLRAQWEMGIVQAEEAASMSVEERKAKFTFLYVGDQLRLNPQVAKEAGIATEPL GEMVDSSVANEEAAVDPGTMREEDIPVKRRRRAKRSSSAENQEGDPGTEKSTPPKMADAEPKRGVGSPAG RKRSTGSASRSRKGDSAAQFLVFCQKHRDEVVAEHPDASEEEIEELLGSQWSMLNEKQKARYNTKFSLMI SAQSEEDSGNTSGKKRTHTKRTDDPPEDVDVEDAPRKRLRTDKHSLRKQRETITDKTARTSSYKAIEAAS SLKSQAATKNLSDACKPLKKRNRASATASSALGFNKSSSPSASLTENEVSDNPGDEPSESPYESADETQT EASVSSKKSERGMAAKKEYVCQLCEKTGSLLLCEGPCCGAFHLACLGLSQRPEGRFTCTECASGIHSCFV CKESKMEVKRCMVNQCGKFYHEACVKKYPLTVFESRGFRCPLHSCMSCHASNPSNPRPSKGKMMRCVRCP VAYHGGDACLAAGCSVIASNSIICTGHFTARKGKRHHTHVNVSWCFVCSKGGSLLCCEACPAAFHPDCLS IEMPDGSWFCNDCRAGKKLHFQDIIWVKLGNYRWWPAEVCHPKNVPPNIQKMKHEIGEFPVFFFGSKDYY WTHQARVFPYMEGDRGSRYQGVRGIGRVFKNALQEAEARFNEIKLQREARETQESERKPPPYKHIKVNKP YGKVQIYTADISEIPKCNCKPTDENPCGSDSECLNRMLMFECHPQVCPAGEYCQNQCFTKRQYPETKIIK TDGKGWGLVAKRDIRKGEFVNEYVGELIDEEECMARIKYAHENDITHFYMLTIDKDRIIDAGPKGNYSRF MNHSCQPNCETLKWTVNGDTRVGLFAVCDIPAGTELTFNYNLDCLGNEKTVCRCGASNCSGFLGDRPKTS TSLSSEEKSKKAKKKTRRRRAKGEGKRQSEDECFRCGDGGQLVLCDRKFCTKAYHLSCLGLGKRPFGKWE CPWHHCDVCGKPSTSFCHLCPNSFCKEHQDGTAFRSTQDGQSYCCEHDLRADSANNTKTEKPFLDSLKAK GKRKKRRCWRRVTDGK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191552
ORF Size	4038 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191552.1, NP_001178481.1
RefSeq Size	5698 bp
RefSeq ORF	4041 bp
Locus ID	680537
MW	150.4 kDa

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