Nsd2 (NM_001191552) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1565590; Whsc1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217596 representing NM_001191552 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCAGGCACCAGAAATCCTTGGCAGTGCAAATGGAAAGACTCAGAACTGTGAAGTGAATCACGAAT GTTCTGTATTCCTCAGCAAAGCTCAGCTTTCTAACAGCCTACAGGAGGGGGTCATGCAGAAATTTAACGG GCATGATGCACTCCCCTTTCTTCCAGCAGAGAAGTTGAAAGATCTTACTTCTTGTGTGTTTAATGGAGAA CCTGGTGCTCATGATACTAAGTTGTGTTTTGAGACCCAGGAAGTAAAAGGAATTGGGACACCACCCAATA CTACTCCTATCAAAAATGGCTCTCCAGAAATTAAACTGAAAATCACCAAAACATACATGAATGGGAAACC TCTCTTTGAATCTTCAATTTGTGGTGATGGCGCTGCTGATATGTCTCAGTCAGAAGAAAATGGACAAAAA TCGGATAATAAGACTAGGAGGAACAGGAAGAGGAGCATAAAATATGACTCTTTGCTGGAGCAGGGCCTTG TGGAAGCAGCTTTAGTGTCCAAGATCTCAAGTCCTGAAGATAAAAAGATTCCAGTTAAGAAGGAGTCCTG TCCAAATAGTGGCAGAGACAGAGACCTCTTGTTAAAATACAACGTTGGTGATTTGGTGTGGTCCAAAGTG TCAGGTTACCCTTGGTGGCCTTGCATGGTTTCTGCTGATCCACTCCTTCACAATCATACCAAACTTAAAG GTCAGAAAAAAAGTGCACGCCAGTATCATGTACAATTCTTTGGTGATGCCCCAGAAAGAGCTTGGATATT TGAGAAGAGCCTTGTTGCTTTTGAAGGAGAAGAACAATTTGAAAAATTATGCCAAGAAAGTGCCAAGCAG GCACCCACGAAAGCTGAGAAAATCAAGTTGTTGAAACCTATTTCAGGGAGATTGAGAGCCCAGTGGGAAA TGGGCATTGTCCAGGCTGAGGAAGCTGCTAGCATGTCAGTAGAGGAGCGGAAAGCCAAGTTTACTTTCCT TTATGTGGGGGACCAACTGCGCCTCAACCCTCAAGTGGCTAAGGAGGCTGGCATTGCTACAGAACCTTTG GGAGAAATGGTGGACTCTTCAGTGGCCAATGAAGAAGCTGCTGTAGACCCTGGGACTATGAGAGAAGAGG ACATTCCCGTAAAGAGAAGGCGAAGAGCCAAAAGGTCTAGTTCTGCTGAGAACCAGGAAGGAGACCCCGG CACAGAAAAGAGTACACCTCCAAAGATGGCGGATGCTGAACCTAAGAGAGGCGTAGGTTCTCCTGCCGGG AGGAAAAGGTCCACAGGATCTGCCTCACGGAGCAGAAAGGGAGACTCAGCAGCCCAGTTCTTAGTCTTTT GTCAAAAACACAGGGATGAGGTCGTAGCTGAACACCCAGATGCCTCAGAGGAGGAGATTGAAGAATTGCT TGGGTCCCAATGGAGCATGCTCAATGAAAAGCAGAAAGCACGATATAATACAAAGTTTTCCCTAATGATC TCTGCCCAGTCTGAAGAAGACTCTGGAAATACCAGTGGGAAAAAAAGGACCCACACAAAAAGGACAGATG ACCCTCCTGAGGATGTGGATGTAGAAGACGCACCCAGGAAAAGACTTAGGACAGATAAGCACAGTCTTCG GAAGCAAAGAGAGACAATCACCGACAAAACGGCCAGAACGAGCTCGTACAAGGCTATAGAGGCAGCCTCC TCACTCAAGAGCCAGGCAGCAACGAAAAATTTGTCTGATGCTTGCAAACCACTGAAGAAGCGAAATCGGG CTTCTGCAACAGCGTCTTCAGCACTTGGGTTTAACAAAAGTTCATCTCCTTCTGCATCCTTAACTGAGAA TGAGGTCTCAGACAACCCAGGAGATGAACCGTCAGAGTCTCCGTATGAAAGTGCAGATGAAACACAGACT GAAGCGTCCGTCTCATCTAAGAAATCTGAAAGAGGAATGGCTGCCAAAAAGGAGTACGTGTGTCAGCTGT GTGAGAAGACAGGCAGTCTCTTACTGTGTGAAGGCCCCTGTTGTGGAGCATTCCACTTAGCCTGCCTTGG ACTTTCCCAGAGACCAGAAGGAAGATTCACCTGCACTGAATGTGCCTCAGGGATTCACTCATGCTTCGTA TGTAAAGAAAGCAAGATGGAGGTGAAACGCTGTATGGTAAACCAATGTGGAAAGTTTTACCATGAAGCCT GTGTGAAAAAATACCCTCTCACTGTGTTTGAGAGCCGAGGCTTTCGCTGTCCTCTCCACAGCTGTATGAG CTGCCATGCCTCAAACCCTTCAAATCCGCGGCCATCAAAAGGTAAAATGATGCGGTGCGTCCGATGCCCT GTCGCCTATCATGGCGGGGATGCCTGCCTGGCAGCGGGATGTTCCGTGATTGCTTCTAACAGCATAATCT GCACAGGCCACTTCACTGCCCGCAAGGGGAAGCGGCACCACACTCATGTCAATGTGAGCTGGTGCTTCGT GTGCTCCAAAGGGGGAAGCCTTCTGTGCTGCGAGGCCTGTCCGGCAGCCTTCCACCCTGATTGTTTGAGC ATCGAGATGCCTGATGGCAGCTGGTTCTGCAATGACTGCCGAGCTGGGAAGAAGCTCCACTTTCAAGACA TCATTTGGGTCAAACTTGGGAACTACAGATGGTGGCCAGCAGAAGTTTGCCATCCCAAAAATGTTCCCCC AAATATTCAGAAAATGAAGCACGAGATTGGAGAATTCCCTGTGTTTTTCTTCGGGTCTAAAGATTATTAC TGGACGCATCAGGCACGAGTGTTCCCATACATGGAGGGGGACCGGGGCAGCCGCTACCAGGGGGTCAGAG GGATTGGAAGAGTCTTCAAGAACGCATTGCAAGAAGCCGAAGCTCGTTTTAATGAAATCAAACTTCAGCG GGAAGCCCGAGAAACACAGGAGAGTGAGAGAAAGCCTCCACCATACAAGCATATCAAGGTGAATAAACCT TATGGTAAAGTCCAGATCTATACAGCTGACATTTCTGAAATCCCAAAATGCAACTGTAAGCCCACGGATG AGAACCCCTGTGGCTCTGATTCAGAGTGTCTAAACAGGATGCTAATGTTTGAGTGCCACCCGCAGGTGTG CCCTGCAGGGGAATATTGCCAGAACCAGTGTTTCACTAAGCGCCAGTACCCGGAGACCAAGATCATCAAG ACCGATGGCAAAGGGTGGGGCCTGGTTGCCAAAAGGGACATCAGAAAGGGAGAATTTGTTAATGAGTATG TTGGCGAACTGATTGATGAAGAGGAGTGTATGGCAAGAATCAAATATGCACATGAGAATGACATCACTCA CTTTTACATGCTCACCATAGACAAGGACCGCATAATTGATGCTGGCCCCAAAGGGAATTACTCGCGGTTC ATGAACCATAGCTGCCAGCCCAACTGTGAGACCCTGAAGTGGACAGTGAATGGGGACACACGGGTTGGCC TGTTTGCTGTGTGCGACATCCCTGCAGGTACAGAGCTGACTTTCAACTATAACCTCGATTGTTTGGGCAA TGAAAAGACAGTCTGTCGGTGTGGAGCCTCCAACTGTAGTGGGTTCCTTGGAGACAGACCAAAGACATCA ACATCCCTTTCTTCAGAGGAAAAGAGTAAAAAGGCCAAGAAGAAAACCAGGAGGCGCAGAGCCAAGGGTG AAGGAAAGCGGCAGTCAGAGGACGAATGCTTCCGCTGTGGTGATGGCGGACAACTGGTGCTGTGTGACCG CAAGTTCTGCACCAAGGCCTACCACCTATCCTGCTTGGGCTTGGGCAAGCGGCCCTTTGGGAAGTGGGAA TGTCCTTGGCATCACTGTGATGTGTGTGGCAAACCTTCTACCTCATTTTGCCACCTCTGCCCCAACTCAT TCTGTAAGGAACACCAAGATGGGACTGCTTTCCGCTCCACCCAGGATGGGCAATCCTACTGCTGTGAGCA TGACCTGAGGGCAGACTCTGCAAATAATACCAAGACTGAGAAACCCTTTCTAGACTCACTCAAGGCGAAG GGGAAGAGGAAGAAAAGGCGGTGCTGGCGAAGGGTCACAGACGGCAAATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191552
Insert Size	4041 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191552.1, NP_001178481.1
RefSeq Size	5698 bp
RefSeq ORF	4041 bp
Locus ID	680537

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