Ttc21b (NM_001191737) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1565122
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217588 representing NM_001191737 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTCCCAGGGGCTGAAGACACTGATCAATTACTATTGTCAAGAGAGATACTATCACCATGTGCTCC TTGTGGCCAGTGAAGGGATGAAGAAGTATAGCAGTGACCCAGTCTTCCGATTTTACCATGCCTACGGCAC ACTAATGGAGGGTAAAGCTCAAGAAGCCCTTCGGGAGTTTGAGGCCATTAAAAATAAACAAGACGTGTCA CTGTGTTCCCTGATGGCGCTGATGTATGTCCATAAAATGAGCCCCAATCCAGATAGAGAAGCTATCCTGG AATTAGATACCAAAATGAAGGAGCAACGTAAGGAAGCCGGACGCAAAGCCTTGTACCATGCAGGCCTGTT TCTGTGGCACATTGGTCGCCATGATAAGGCGAGAGAATATATTGACAGAATGTCAAAAATGCCACATGAT AGCAATGAGGGACCGATTTTGAAAGCATGGCTTGATATTACAAGAGGGAAGGAACCTTATGCTAAAAAAG CCCTACGGTATTTTGAGGAGGGATTACAAGATGGAAACGATATTTTTGCTCTGCTGGGTAAGGTACTGTG CCTTGAGATGCGACAGAATTATTCTGGAGCTCTGGAGACTGTGAGTCAGATAATTGTGAACTTCCCGAGC TTCCTTCCTGCTTTTGAGAAGAAAATGAAATTACAACTGGCTTTGCAGGATTGGGATCAGACGGTGGAGA CGGCGCAAAGGTTGCTGCTTCAGGACGGCCACAATGTGGAGGCGCTGAGAATGCTGGCTTTATATTATCT GTGTAGGGAAGGGGACATAGAGAAGGCTGCTGCCAAGCTGGAAAATTTAGGAAATGCATTGGATGTCATG GAACCACAGAATGCTCAACTTTTTTATAAGATTACAATAGCCTTCAGCAGAACTTGTGGACGTAATCAAC TCATTCTTCAAAAAGTTCAAAGTTTCCTAGAAAAAGCATTTAGTTTAACCCCCCAGCAAGCAGAAATTGC TACAGAGCTTGGCTACCAAATGATTCTCCAAGGCAAAGTCAAGGAGGCCTGGAAGTGGTACAGGACCGCC ATGACACTGAACGAAAGCAACATCTCTGCCGTCACCGGACTTATCCGATGTCAGTTAATAGAAGGACAAT TGCAAGATGCAGACCAGCAGTTGGAGTTCTTCAGTGAATTCCAGCAGTCAATGGGAAGATCTGCGGAATT AATGTATTTGCATGCGGTTCTTGCTACCAAAAAAAATAATCGTCAGGAGGAAGTTATAAATTTGTTGAAT GATGTTGTGAACACTCACTTTTCACACTTGGAAGACCTCCCTCTCGGCATACAGTATTTTGAGAAGCTCA ACCCTGACTTCTTACTAGAAGTTGTTAACGAGTATCTGAATCTCTGTCCAATTCAGCCTGCAGGTCCTGG GCAGCCTCTCTCCCCAGTCCTCAGACGTTGTTCTTCAGTTCTGGAGACGATTATAAGAAGTGTACCAGGT CTTCCGCAAGCTGTCTTCCTAATGGCCAAAGTGAAGTATCTGTCAGGTGACACCGAGGCAGCCTACAACA ACCTGCAGCACTGCCTGGAGCACAGCCCCTCTTACGCAGAGGCTCACCTCCTGATGGCGCAGGTGTACCT ATCCCAAGACAAAGTCCAGCTGTGTTCTCAGTCGCTTGAACTTTGTCTGAGCTACAATTTCAATGTGAGA GACTACCCACTGTACCACCTAATCAAAGCACAGTCACAGAAGAAAATGGGCGAGGTAGCAGAAGCAATTA AAACTCTGCACATGGCAATGAATCTACCAGGAATGAGGAGAAGTAGAGCTTCCTCGAAATCCAAGCACAG AAGTGAAGTGGATGGGAGCCATCGCTTATCCATCTTTCTTGAGTTGGTGGAGGTTCACCGCTTAAATGGA GAACAGCACGAGGCAGCAAAGGTTTTACAAGATGCCATCCATGAGTTTTCTGGAACTTGCGAAGAATTAC GTGTCACTATTGCTAATGCAGACCTGGCTCTGGCCCAAGGAGATACAGAACGTGCATTAAGCATGCTCCG AAATGTCACGACCGAACAGCCTTATTTCATAGAAGCCAAGGAAAAAATGGCAGACATTTACCTGAAGCAT AGGAAAGAGAAAATGTTGTACATCACTTGTTACAGAGAAATTGCTGAAAGGATGCCCAGCCCCCGGTCTT TCCTGCTCCTTGGTGATGCATACATGAACATTCAAGAGCCCGAGGAAGCCATAGTGGCCTATGAGCAAGC ATTAAATCAGAACCCAAAAGATGGAACACTGGCGAGAAAAATTGGGAAAGCACTTGTCAAAACTCACAAC TACTCAAAGGCAATCACCTATTATGAGGCTGCGCTGAAAAGTGGACAGCAGAATTGCCTGTGCTATGACC TGGCTGAGCTCCTCCTGAGACTAAAGTTGTATGAGAAAGCAGAGAAAGTTCTTCAGCATTCTCTAGCTCA TGATCCTGTAAATGAATTGTCGGCTCTCATGGTGGATGGGCGTTCCCAAGTCCTTCTAGCAAAAGTTTAC TCTAAAATGGAGAGACCTGGCGACGCGATTGCAGCATTGCAGCAGGCTCGAGAGCTGCAGGCTCGGATAC TGAAGCGAGTTCAGATGGAGCAGCCAGATGCGGTTCCCTCACAGAGACACTTCGCAGCTGAAATTTGTGC AGAGATCGCAAAACACTCCGCTGCTCAGCGAGACTACGAAAAGGCAATTACATTTTATAGAGAGGCCCTG GTTCATTGTGAAACGGATAGTAAGATAATGTTGGAACTGGCCCAGTTATACCTGGCCCAAGAAGACCTTG ATGCCTCCCTCCGGCACTGTGCACTGCTTCTCCAGAGGGACCAGGACAACGAACCGGCCACCATGCTGAT GGCCGACCTCATGTTCAGAAAACAGGACTATGAGCAAGCAGTGTTCCACTTGCAGCAGCTTTTAGAACGA AAACCAGATAATTTTATGACTTTGTCCCGTTTGATTGATCTCCTGAGAAGATGTGGGAAACTTGAGGAGG TTCCGAGATTTTTCTTGATGGCTGAGAAACACAACTCCAGAACAAAATTGGAGCCAGGATTTCAGTACTG TAAAGGATTACATTTTTGGTATACTGGAGAACCAAATGATGCCCTTCGACATTTTAATAAAGCTCGGAAA GATAGTGACTGGGGTCAAAATGCACTTTATAATATGATAGAAATCTGCTTGAATCCAGACAATGAAACTA TTGGAGGTGAAGTGTTTGAAAACCTGAATGGAGACCTGGGCACTTCCCCAGAGAAGCAGGAATCTGTGCA GTTAGCAGTGAGGACGGCAGAAAAGCTCCTTAAAGAACTAAAACCTCAGACTGTCCAGGGCCGCCTGCAA CTCCGCATCATGGAAAACTGCTGCTTAATGGCCACCAAGCAGAAGTCCAGTGTGGAGCAGGCGCTGAACA CCTTCACCGAAATAGCAGCCTCTGAGAAGGATCATATCCCTGCTCTCCTGGGAATGGCCACCGCTTATAT GATCTTGAAACAGACCCCGAAAGCCAGAAACCAGCTGAAGCGGATTGCAAAAATGACCTGGAATCCTATT GAAGCCGAGGAGCTGGAGAAGAGCTGGCTGCTGCTTGCGGATATTTATATCCAGTCAGCAAAATATGACA TGGCGGAAGAGCTGCTGAAACGGTGTCTGTGCCATAACCGGTCCTGCTGCAAAGCTTATGAATATATGGG GTACATTATGGAGAAGGAGCAAGCATACACAGATGCAGCCTTCAACTATGAGATGGCCTGGAAACATAGC AACCAGACAAATCCTGCTGTGGGATATAAATTGGCATTTAATTACTTAAAAGCAAAACGATATGTGGATG CAATTGACGTATGTCACCAGGTTCTTGAAGCACATCCAACTTACCCCAAAATCCGAAAGGATATACTGGA TAAGGCCCGTGCATCGTTAAGACCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217588 representing NM_001191737 Red=Cloning site Green=Tags(s) MDSQGLKTLINYYCQERYYHHVLLVASEGMKKYSSDPVFRFYHAYGTLMEGKAQEALREFEAIKNKQDVS LCSLMALMYVHKMSPNPDREAILELDTKMKEQRKEAGRKALYHAGLFLWHIGRHDKAREYIDRMSKMPHD SNEGPILKAWLDITRGKEPYAKKALRYFEEGLQDGNDIFALLGKVLCLEMRQNYSGALETVSQIIVNFPS FLPAFEKKMKLQLALQDWDQTVETAQRLLLQDGHNVEALRMLALYYLCREGDIEKAAAKLENLGNALDVM EPQNAQLFYKITIAFSRTCGRNQLILQKVQSFLEKAFSLTPQQAEIATELGYQMILQGKVKEAWKWYRTA MTLNESNISAVTGLIRCQLIEGQLQDADQQLEFFSEFQQSMGRSAELMYLHAVLATKKNNRQEEVINLLN DVVNTHFSHLEDLPLGIQYFEKLNPDFLLEVVNEYLNLCPIQPAGPGQPLSPVLRRCSSVLETIIRSVPG LPQAVFLMAKVKYLSGDTEAAYNNLQHCLEHSPSYAEAHLLMAQVYLSQDKVQLCSQSLELCLSYNFNVR DYPLYHLIKAQSQKKMGEVAEAIKTLHMAMNLPGMRRSRASSKSKHRSEVDGSHRLSIFLELVEVHRLNG EQHEAAKVLQDAIHEFSGTCEELRVTIANADLALAQGDTERALSMLRNVTTEQPYFIEAKEKMADIYLKH RKEKMLYITCYREIAERMPSPRSFLLLGDAYMNIQEPEEAIVAYEQALNQNPKDGTLARKIGKALVKTHN YSKAITYYEAALKSGQQNCLCYDLAELLLRLKLYEKAEKVLQHSLAHDPVNELSALMVDGRSQVLLAKVY SKMERPGDAIAALQQARELQARILKRVQMEQPDAVPSQRHFAAEICAEIAKHSAAQRDYEKAITFYREAL VHCETDSKIMLELAQLYLAQEDLDASLRHCALLLQRDQDNEPATMLMADLMFRKQDYEQAVFHLQQLLER KPDNFMTLSRLIDLLRRCGKLEEVPRFFLMAEKHNSRTKLEPGFQYCKGLHFWYTGEPNDALRHFNKARK DSDWGQNALYNMIEICLNPDNETIGGEVFENLNGDLGTSPEKQESVQLAVRTAEKLLKELKPQTVQGRLQ LRIMENCCLMATKQKSSVEQALNTFTEIAASEKDHIPALLGMATAYMILKQTPKARNQLKRIAKMTWNPI EAEELEKSWLLLADIYIQSAKYDMAEELLKRCLCHNRSCCKAYEYMGYIMEKEQAYTDAAFNYEMAWKHS NQTNPAVGYKLAFNYLKAKRYVDAIDVCHQVLEAHPTYPKIRKDILDKARASLRP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191737
ORF Size	3945 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191737.1, NP_001178666.1
RefSeq Size	3948 bp
RefSeq ORF	3948 bp
Locus ID	295654
MW	150.8 kDa

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