Slc4a7 (NM_001270860) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	NBC3; NBCn1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217560 representing NM_001270860 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCAGACGGGGCCGGCGAGCAGATGAGACCGCTACTCACGCGGGGTCCCGATGAAGAAGCTGTTG TGGATCTTGGCAAAACTAGCTCAACCGTGAACACCAAGTTTGAAAAAGAAGAATTAGAGAGTCATCGAGC TGTATATGTTGGTGTTCATGTACCGTTTAGTAAAGAGAGTCGTCGGCGTCACAGGCATCGAGGGCACAAA CATCACCACCGGAGAAGAAAAGACAAAGACTCAGATAAGGAAGATGGACGGGAGTCTCCTTCTTATGACA CGCCATCGCAGAGGGTGCAGTTCATCCTTGGGACTGAAGACGATGATGAGGAGCACATTCCCCACGACCT CTTCACGGAGATGGACGAGCTCTGCTACCGAGACGGGGAAGAGTACGAGTGGAAAGAGACAGCCAGGTGG CTGAAGTTCGAAGAGGATGTTGAGGATGGTGGTGACCGATGGAGTAAACCGTATGTGGCCACTCTGTCTT TGCACAGTCTCTTTGAGCTGAGGAGTTGTATCCTAAATGGAACAGTCATGCTGGATATGAGAGCAAGCAC TCTGGATGAAATAGCAGATATGGTGTTAGACAACATGATAGCGTCTGGCCAGCTAGATGACTCCATAAGG GAGAATGTTCGAGAAGCTCTTCTGAAGAGACATCATCATCAAAATGAGAAAAGGTTTACAAGTCGGATTC CCCTCGTTCGATCCTTTGCAGATATAGGGGAAGGCCTGTCAGCCTCCCGCCATTCTTTGCGAACAGGTCT GTCTGCCTCAAACCTTTCCTTGAGAGGAGAATCGCCTTTATCCCTTCTTCTCAGTCATCTTCTTCCTTCT TCAAGAGCTGGCACCCCTGCAGGCTCAAGGTGTACCACCCCAGTACCCACCCCCCAGAACAGTCCTCCTT CCAGCCCCAGCTTAAGTCGCCTGACCTCCAGAAGTTCCCAACAGACTCAGCCTCAGGCCCCAGAAGTACT GGTGTCACCTGACAGGGATGATATTCCCAGAGTAGTAATTCATCCGCCTGAGGAAGACATAGAAGCACTG AAAGGCCAAGAGCAGAAGAATGAGGAAAATACTGACTTCACTCCAGGGATTTTGGCTTCTCCACAGTCTG CTCCTGGAAACCTGGACAGTAGTAAAAGTGGTGAAGTGAAAGGTAACGGAAGTGGAGGAAGCAGAGAAAA TAGTACTGTTGACTTTAGCAAGGTTGACATGAATTTCATGAGGAAAATTCCTACAGGAGCTGAGGCATCC AATGTTTTGGTAGGAGAGGTGGATTTCTTGGAGAGACCTATCATTGCATTTGTGAGACTGGCTCCTGCAG TTCTCCTCTCAGGGTTGACTGAGGTCCCTGTGCCCACTAGGTTTTTGTTTCTGTTACTGGGCCCAGCAGG AAAGGCTCCACAGTACCATGAAATTGGCAGATCCATAGCAACTCTAATGACAGATGAGATTTTTCATGAT GTAGCTTATAAAGCAAAAGATCGAAATGACCTCTTATCTGGAATTGATGAATTTTTAGATCAAGTAACTG TTCTTCCTCCAGGAGAGTGGGATCCTTCCATACGCATAGAGCCACCAAAAAGTGTTCCTTCTCAGGAGAA AAGGAAGATTCCTGCGTTTCCCAATGGATCTGCTCCAGTGTCTGCTGACCCTCCTAAGGAGGCTGATCAC CACGCTGGGCCTGAGCTGCAGAGGACTGGACGGCTTTTTGGTGGTTTGATACTTGACATCAAAAGGAAAG CACCTTTTTTCTTGAGTGACTTCAAGGATGCATTAAGTCTGCAGTGCCTGGCCTCGATTCTTTTCCTATA CTGTGCCTGTATGTCTCCTGTAATCACTTTTGGAGGGCTGCTTGGAGAAGCTACAGAAGGCAGAATAAGT GCAATAGAGTCTCTTTTTGGAGCATCATTAACTGGGATTGCCTATTCATTGTTTGCTGGGCAACCTCTAA CAATACTGGGGAGCACGGGTCCAGTCCTAGTGTTTGAAAAAATTTTATTTAAATTCTGTAGAGATTATCA CCTATCCTATCTATCATTAAGAACCAGTATTGGTCTGTGGACTTCTTTCTTGTGCATTGTGTTGGTCGCA ACAGATGCCAGCAGCCTTGTTTGTTACATTACTCGGTTCACAGAAGAAGCTTTCGCCGCACTCATTTGTA TCATCTTCATCTATGAAGCCTTGGAGAAACTCTTTCACTTAGGAGAAATATATGCATTTAACATGCACAA CAACTTGGATGCATTGACCAGTTACACATGTGTATGTGCTGAGCCATCTAATCCTAGCAACGAAACTGTA GAGCTGTGGGAGAGGAAGAACGTGACAGCAGCCAGTATTTCCTGGGCAAACCTTACCGTGTCTGAGTGTA AGACCTTCCACGGTATGTTTGTGGGATCAGCTTGTGGGCCTCACGGGCCTTATGTTCCCGATGTGCTCTT CTGGTGTGTCGTCTTGTTTTTCACAACGTTCTTTCTGTCTTCATTCCTCAAGCAGTTTAAGACCAAGAGA TATTTTCCTACTAAGGTGCGATCAACAATCAGTGACTTTGCTGTATTTCTCACAATAGTAATAATGGTTG CAATTGACTACCTTGTAGGAATTCCATCTCCTAAACTTCATGTTCCTGAAAAGTTTGAGCCTACTGATCC AAGCAGGGGCTGGATCATAAGCCCTTTGGGAGATAACCCTTGGTGGACCTTACTAATTGCAGCTGTTCCA GCTCTCCTTTGTACCATTCTCATCTTCATGGACCAGCAGATCACAGCTGTGATCATCAACAGGAAAGAAC ACAAACTGAAGAAAGGAGCTGGCTATCACCTGGACTTGCTCATGGTTGCTGTCATGTTGGGAGTCTGCTC CATCATGGGCCTGCCGTGGTTTGTGGCTGCAACAGTGTTGTCTATAAGTCATGTCAACAGCTTAAAAGTA GAGTCTGAATGTTCTGCTCCAGGGGAACAGCCCAAGTTCCTCGGAATTCGTGAACAGCGGGTTACAGGGC TGATGATTTTTATCCTGATGGGCCTCTCTGTGTTCATGACTTCAGTATTAAAGTTTATTCCGATGCCAGT TCTATATGGTGTTTTCCTTTATATGGGAGTTTCTTCTCTGAAAGGAATTCAGTTTTTTGACCGTATCAAA TTATTTGGAATGCCTGCCAAGCACCAGCCGGACCTGATCTACCTCCGCTATGTCCCTCTCTGGAAGGTGC ACGTGTTCACGGTCGTCCAGCTGACCTGCCTGGTTCTGCTCTGGGTGATCAAAGCCTCTGCTGCTGCAGT AGTTTTTCCCATGATGGTTCTTGCATTAGTCTTTGTGCGCAAGCTCATGGATCTGTGCTTCACAAAGAGA GAACTCAGTTGGCTTGATGACCTCATGCCAGAAAGTAAGAAAAAGAAAGAAGATGACAAGAAGAAGAAGG AGAAGGAGGAAGCTGAGCGGATGCTTCAGGGTGACGGGGATACTGTGCACCTCCCATTCGAAAGGGGGAG TCTCCTACAAATTCCAGTTAAGACCCTAAAATATAGTATTGACCCTTCAGTTGTTAACATATCAGATGAA ATGGCCAAAACTGCCCAGTGGAAGGCACTTTCCATGAATACTGAGAATGCCAAAGTAACCAGACCTAACA TGAGCCCTGAAAAGCCTGTGAGTGTGACAATAAATTTCGAAGATGAACCATCAAAAAAATACATGGATGC TGAAACTTCATTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217560 representing NM_001270860 Red=Cloning site Green=Tags(s) MEADGAGEQMRPLLTRGPDEEAVVDLGKTSSTVNTKFEKEELESHRAVYVGVHVPFSKESRRRHRHRGHK HHHRRRKDKDSDKEDGRESPSYDTPSQRVQFILGTEDDDEEHIPHDLFTEMDELCYRDGEEYEWKETARW LKFEEDVEDGGDRWSKPYVATLSLHSLFELRSCILNGTVMLDMRASTLDEIADMVLDNMIASGQLDDSIR ENVREALLKRHHHQNEKRFTSRIPLVRSFADIGEGLSASRHSLRTGLSASNLSLRGESPLSLLLSHLLPS SRAGTPAGSRCTTPVPTPQNSPPSSPSLSRLTSRSSQQTQPQAPEVLVSPDRDDIPRVVIHPPEEDIEAL KGQEQKNEENTDFTPGILASPQSAPGNLDSSKSGEVKGNGSGGSRENSTVDFSKVDMNFMRKIPTGAEAS NVLVGEVDFLERPIIAFVRLAPAVLLSGLTEVPVPTRFLFLLLGPAGKAPQYHEIGRSIATLMTDEIFHD VAYKAKDRNDLLSGIDEFLDQVTVLPPGEWDPSIRIEPPKSVPSQEKRKIPAFPNGSAPVSADPPKEADH HAGPELQRTGRLFGGLILDIKRKAPFFLSDFKDALSLQCLASILFLYCACMSPVITFGGLLGEATEGRIS AIESLFGASLTGIAYSLFAGQPLTILGSTGPVLVFEKILFKFCRDYHLSYLSLRTSIGLWTSFLCIVLVA TDASSLVCYITRFTEEAFAALICIIFIYEALEKLFHLGEIYAFNMHNNLDALTSYTCVCAEPSNPSNETV ELWERKNVTAASISWANLTVSECKTFHGMFVGSACGPHGPYVPDVLFWCVVLFFTTFFLSSFLKQFKTKR YFPTKVRSTISDFAVFLTIVIMVAIDYLVGIPSPKLHVPEKFEPTDPSRGWIISPLGDNPWWTLLIAAVP ALLCTILIFMDQQITAVIINRKEHKLKKGAGYHLDLLMVAVMLGVCSIMGLPWFVAATVLSISHVNSLKV ESECSAPGEQPKFLGIREQRVTGLMIFILMGLSVFMTSVLKFIPMPVLYGVFLYMGVSSLKGIQFFDRIK LFGMPAKHQPDLIYLRYVPLWKVHVFTVVQLTCLVLLWVIKASAAAVVFPMMVLALVFVRKLMDLCFTKR ELSWLDDLMPESKKKKEDDKKKKEKEEAERMLQGDGDTVHLPFERGSLLQIPVKTLKYSIDPSVVNISDE MAKTAQWKALSMNTENAKVTRPNMSPEKPVSVTINFEDEPSKKYMDAETSL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001270860
ORF Size	3723 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001270860.1, NP_001257789.1
RefSeq Size	4002 bp
RefSeq ORF	3726 bp
Locus ID	117955
MW	138.4 kDa
Gene Summary	regulates intracellular pH in different cells along with Na+-driven HCO3- transporters, the Na+-driven Cl-/HCO3- exchanger [RGD, Feb 2006]

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