Ctr9 (NM_001100661) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Sh2bp1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217525 representing NM_001100661 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGCGGGGTTCCATCGAGATTCCCCTCCGGGATACTGACGAGGTCATTGAACTTGACTTCGATCAGT TACCGGAGGGGGATGAAGTTATCAGTATTCTGAAGCAGGAGCACACACAACTGCACATATGGATTGCTTT GGCGCTGGAGTACTACAAGCAAGGGAAAACAGAAGAGTTTGTGAAGCTGCTGGAGGCTGCACGGATAGAT GGCAATTTGGACTATCGAGACCACGAGAAAGACCAGATGACTTGCTTGGATACATTGGCAGCCTACTATG TACAACAGGCTCGGAAAGAAAAGAACAAAGACAATAAGAAAGACCTTATTACGCAGGCAACTTTGTTGTA TACAATGGCTGATAAAATTATTATGTATGATCAGAACCATTTGTTGGGAAGAGCCTGCTTCTGCCTGCTC GAGGGTGACAAAATGGACCAAGCTGACGCACAGTTTCATTTTGTACTTAACCAGTCTCCAAACAATATTC CAGCCCTTCTTGGGAAAGCTTGTATCTCATTCAACAAGAAGGACTACAGAGGAGCCCTTGCTTACTATAA AAAAGCATTGCGCACTAACCCAGGATGTCCAGCTGAAGTTCGTCTAGGAATGGGCCATTGCTTTGTCAAA CTGAACAAACTGGAAAAAGCCCGACTAGCATTCAGCAGAGCCCTGGAACTCAACTCTAAGTGTGTGGGAG CACTGGTCGGACTGGCTGTTCTAGAACTCAACAATAAAGAGGCTGATTCCATCAAAAATGGTGTGCAGCT TCTTTCCAGAGCCTACACTATTGATCCTAGCAACCCTATGGTACTGAACCATTTGGCAAACCACTTTTTC TTCAAGAAGGATTATAGTAAAGTGCAGCACCTGGCTCTCCACGCGTTCCACAACACAGAGGTGGAGGCCA TGCAAGCAGAGAGCTGTTACCAGCTGGCTCGGTCCTTCCATGTTCAGGAAGATTATGACCAGGCCTTTCA GTACTACTATCAAGCCACACAGTTTGCTTCATCTTCTTTTGTCCTACCATTTTTTGGTTTGGGACAAATG TATATTTATCGAGGTGACAAAGAAAATGCATCTCAGTGTTTCGAGAAAGTTTTGAAAGCTTATCCGAACA ATTATGAAACTATGAAAATTCTTGGCTCTCTCTATGCTGCTTCAGAAGATCAGGAGAAAAGAGACATAGC CAAGGGCCATTTGAAGAAGGTTACAGAACAGTATCCTGATGACGTGGAAGCTTGGATTGAGTTGGCCCAG ATCTTAGAACAGACTGACATACAGGGTGCCCTTTCAGCCTATGGAACGGCAACACGAATCCTGCAGGAGA AGGTGCAGGCCGATGTCCCGCCTGAGATTTTGAATAATGTTGGGGCCCTCCATTTTAGACTTGGAAACCT AGGCGAAGCAAAGAAGTACTTCCTGGCCTCACTGGACCGAGCAAAGGCAGAGGCGGAGCATGATGAACAC TATTACAATGCCATCTCTGTCACCACATCCTACAATTTAGCCAGGCTCTACGAGGCCATGTGTGAATTTC ATGAAGCAGAGAAACTGTACAAAAACATTTTACGAGAGCATCCTAACTATGTTGATTGCTATCTGCGCCT AGGAGCCATGGCCAGAGATAAAGGAAACTTCTATGAAGCTTCAGATTGGTTTAAGGAAGCCCTTCAGATC AATCAGGACCATCCCGATGCCTGGTCTTTGATTGGTAACCTCCATCTGGCAAAACAAGAGTGGGGCCCAG GTCAGAAGAAGTTTGAGAGGATCTTAAAACAGCCAGCCACACAGAGTGACACCTACTCTATGCTGGCCCT TGGCAATGTGTGGCTCCAGACTTTGCATCAGCCAACCCGAGACCGAGAAAAGGAAAAGCGCCATCAAGAC CGTGCGCTGGCCATCTACAAACAAGTCCTGAGAAATGATGCCAAGAACCTGTATGCTGCCAATGGCATAG GAGCTGTCTTGGCCCACAAAGGATATTTCCGTGAAGCTCGTGATGTGTTCGCCCAAGTGAGAGAAGCAAC AGCAGATATCAGTGACGTGTGGCTGAACTTAGCACACATCTACGTCGAGCAGAAGCAGTATATCAGCGCC GTTCAGATGTATGAGAACTGCCTCCGAAAGTTCTATAAGCACCAAAACACTGAAGTTGTGCTCTACCTGG CCCGTGCCCTCTTCAAGTGTGGCAAGTTGCAGGAGTGCAAGCAGACTCTGCTGAAGGCCCGACACGTGGC ACCCAGCGACACAGTTCTGATGTTTAATGTGGCCTTGGTCTTGCAAAGATTAGCTACCTCTGTCCTGAAA GATGAAAAAAGTAATTTGAAGGAAGTCCTTAATGCTGTGAAAGAGCTGGAGCTTGCACACAGATACTTCA GTTACTTGAGTAAAGTTGGAGATAAGATGAGATTTGATTTGGCCCTTGCTGCCTCTGAAGCCAGGCAGTG CTCTGACTTACTGAGCCAGGCCCAATACCATGTGGCTCGGGCACGCAAGCAGGATGAGGAGGAACGGGAG CTGCGGGCCAAGCAGGAGCAGGAGAAGGAGCTCCTAAGACAGAAGCTTCTCAAAGAGCAGGAAGAAAAAC GTCTCAGAGAAAAGGAAGAGCAAAAGAAACTTTTGGAACAGCGGGCCCAGTATGTGGAGAAGACCAAAAA TATCCTCATGTTCACTGGCGAGACTGAAGCAACAAAAGAGAAGAAAAGAGGTGGAGGTGGAGGGCGGCGT TCCAAGAAAGGAGGCGAGTTTGATGAGTTTGTCAACGATGACACTGATGACGACCTCCCTATATCCAAGA AGAAGAAGAAAAGGAAGGGCAGTGGCAGTGAACAGGAAGGCGAAGAGGAGGAAGGTGGAGAGAGGAAGAA GAAGAGGAGGAGAAGACCTCCAAAGGGAGAAGAAGGATCTGATGATGATGAAACAGAAAATGGCCCCAAA CCAAAGAAGCGCCGTCCACCGAGAGCAGAGAAAAAGAAGGCTCCCAAGCCAGAACGCCTGCCTCCTTCAA TGAAAGGAAAAATAAAATCCAAAGCCATTATATCATCAAGCGATGATTCTTCAGATGAGGATAAACTGAA AATTGCTGATGAAGGACATCCCAGGAACAGCAACAGCGACTCTGATGATGACGAGAGGCCCAACAGACGC GCCTCATCGGAGAGCGACTCAGATGACAACCAGAACAAGTCTGGCAGCGAGGCGGGTAGCCCCCGGAGGT CTGGTAGGCAGGAGTCCGATGAGGATTCCGACAGTGACCAGCCGTCCAGAAAGAGAAGGCGCTCCGGCTC TGAGCAGTCGGACAATGAGTCTGCGCAGTCCGGGAGGAGCCCCTCCGGAGGCTCAGAGAATGAGAACGAT TCTCGCCCGGTTTCTCCAAGTGCAGAGTCGGACCACGAATCAGAGCAGGGCTCTGACAACGAAGGTTCTG GCCAAGGCTCTGGAAATGAATCGGAACCAGAGGGCTCAAACAATGAGGCCTCAGACCGAGGCTCAGAACA TGGATCAGATGATAGCGAC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217525 representing NM_001100661 Red=Cloning site Green=Tags(s) MSRGSIEIPLRDTDEVIELDFDQLPEGDEVISILKQEHTQLHIWIALALEYYKQGKTEEFVKLLEAARID GNLDYRDHEKDQMTCLDTLAAYYVQQARKEKNKDNKKDLITQATLLYTMADKIIMYDQNHLLGRACFCLL EGDKMDQADAQFHFVLNQSPNNIPALLGKACISFNKKDYRGALAYYKKALRTNPGCPAEVRLGMGHCFVK LNKLEKARLAFSRALELNSKCVGALVGLAVLELNNKEADSIKNGVQLLSRAYTIDPSNPMVLNHLANHFF FKKDYSKVQHLALHAFHNTEVEAMQAESCYQLARSFHVQEDYDQAFQYYYQATQFASSSFVLPFFGLGQM YIYRGDKENASQCFEKVLKAYPNNYETMKILGSLYAASEDQEKRDIAKGHLKKVTEQYPDDVEAWIELAQ ILEQTDIQGALSAYGTATRILQEKVQADVPPEILNNVGALHFRLGNLGEAKKYFLASLDRAKAEAEHDEH YYNAISVTTSYNLARLYEAMCEFHEAEKLYKNILREHPNYVDCYLRLGAMARDKGNFYEASDWFKEALQI NQDHPDAWSLIGNLHLAKQEWGPGQKKFERILKQPATQSDTYSMLALGNVWLQTLHQPTRDREKEKRHQD RALAIYKQVLRNDAKNLYAANGIGAVLAHKGYFREARDVFAQVREATADISDVWLNLAHIYVEQKQYISA VQMYENCLRKFYKHQNTEVVLYLARALFKCGKLQECKQTLLKARHVAPSDTVLMFNVALVLQRLATSVLK DEKSNLKEVLNAVKELELAHRYFSYLSKVGDKMRFDLALAASEARQCSDLLSQAQYHVARARKQDEEERE LRAKQEQEKELLRQKLLKEQEEKRLREKEEQKKLLEQRAQYVEKTKNILMFTGETEATKEKKRGGGGGRR SKKGGEFDEFVNDDTDDDLPISKKKKKRKGSGSEQEGEEEEGGERKKKRRRRPPKGEEGSDDDETENGPK PKKRRPPRAEKKKAPKPERLPPSMKGKIKSKAIISSSDDSSDEDKLKIADEGHPRNSNSDSDDDERPNRR ASSESDSDDNQNKSGSEAGSPRRSGRQESDEDSDSDQPSRKRRRSGSEQSDNESAQSGRSPSGGSENEND SRPVSPSAESDHESEQGSDNEGSGQGSGNESEPEGSNNEASDRGSEHGSDDSD SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001100661
ORF Size	3519 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001100661.1, NP_001094131.1
RefSeq Size	4234 bp
RefSeq ORF	3522 bp
Locus ID	293184
MW	133.3 kDa

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