Nfasc (NM_001160315) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	NF
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217514 representing NM_001160315 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGGCAGCAGGCGCCACCCTGGGTCCACGTAGCCCTCATCCTCTTCCTCCTCAGCCTCGGAGGGG CCATTGAGATTCCGATGGATCTGACCCAACCCCCAACGATCACCAAGCAGTCGGTGAAGGACCACATCGT GGACCCCCGAGATAACATCCTGATTGAATGTGAAGCTAAAGGGAACCCCGCCCCCAGTTTCCACTGGACT CGCAACAGCAGGTTCTTCAACATTGCCAAGGACCCACGGGTGTCCATGAGGAGGAGGTCTGGGACCTTGG TGATCGACTTCCGCAGTGGTGGGCGGCCTGAGGAGTACGAAGGGGAGTACCAGTGCTTTGCCCGGAACAA ATTCGGCACAGCTCTTAGCAACCGCATCCGCCTGCAGGTGTCCAAATCTCCCCTGTGGCCCAAGGAAAAC CTAGACCCCGTCGTGGTTCAAGAGGGTGCCCCCTTAACCCTGCAGTGCAACCCCCCACCTGGCCTCCCAT CCCCCGTCATCTTCTGGATGAGCAGCTCCATGGAGCCCATCACCCAGGACAAGCGTGTCTCCCAGGGTCA CAACGGGGACCTGTACTTCTCCAACGTCATGCTGCAGGACATGCAGACCGACTACAGCTGCAATGCACGC TTCCACTTCACCCACACCATTCAGCAGAAGAATCCCTTCACCCTCAAAGTCCTCACCACCCGAGGAGTTG CGGAAAGAACACCTAGCTTCATGTATCCCCAGGGCACGTCAAGCAGTCAGATGGTACTGCGCGGCATGGA CCTGCTGCTGGAGTGCATTGCCTCTGGGGTCCCAACACCAGATATTGCATGGTACAAGAAAGGTGGGGAC CTCCCATCTGACAAGGCCAAGTTCGAGAATTTTAACAAGGCTCTGCGCATCACCAATGTCTCTGAAGAGG ACTCTGGGGAGTATTTCTGCCTGGCCTCCAACAAGATGGGCAGCATCCGGCACACGATCTCGGTGAGAGT AAAGGCTGCCCCATACTGGCTGGATGAGCCCAAGAACTTGATCCTGGCTCCTGGTGAAGATGGGAGGCTG GTGTGTCGAGCCAATGGGAACCCGAAGCCGACCGTCCAGTGGCTGGTGAATGGAGACCCTTTGCAATCGG CACCACCCAACCCCAACCGTGAGGTGGCCGGAGACACTATCATCTTCCGGGACACTCAGATCAGCAGCAG GGCAGTGTACCAGTGTAACACATCCAACGAACATGGCTACCTGCTGGCCAATGCCTTCGTCAGCGTATTA GATGTACCCCCTCGGATGCTGTCTCCCCGGAACCAGCTCATCAGGGTGATCCTTTACAACCGGACGCGAC TGGACTGTCCGTTCTTTGGGTCTCCCATTCCAACACTCCGATGGTTTAAGAATGGGCAAGGAAGCAACCT GGATGGTGGTAACTACCACGTCTACGAAAATGGCAGCCTGGAAATCAAGATGATTCGCAAAGAGGACCAA GGCATCTACACCTGTGTGGCCACCAACATCCTGGGAAAAGCTGAAAATCAAGTCCGCCTGGAGGTCAAAG ACCCCACCAGGATCTACAGGATGCCTGAAGACCAGGTGGCCAAGAGGGGCACCACAGTGCAGCTGGAGTG CCGTGTGAAGCATGACCCCTCCTTGAAACTCACAGTCTCCTGGCTGAAGGATGACGAGCCACTCTACATT GGAAACAGGATGAAAAAGGAAGATGACTCCCTGACCATCTTCGGAGTGGCAGAGCGGGACCAAGGCAGTT ACACGTGCATGGCCAGCACCGAGCTGGACCAGGACCTGGCAAAGGCCTACCTCACTGTTCTAGGGCGGCC AGACCGACCCAGGGACCTGGAGCTCACTGACCTGGCGGAAAGGAGTGTGAGGCTGACCTGGATCCCGGGG GATGATAACAACAGTCCCATCACAGACTACGTTGTCCAGTTTGAAGAGGACCAGTTCCAGCCAGGAGTCT GGCATGACCACTCCAAGTTCCCAGGCAGTGTCAACTCAGCCGTCCTCCATCTGTCCCCGTATGTCAACTA TCAGTTCCGAGTCATCGCTGTCAACGAGGTTGGGAGCAGCCACCCCAGCCTTCCATCCGAGCGGTACCGA ACAAGCGGGGCACCCCCTGAATCCAACCCCAGTGATGTGAAGGGCGAAGGGACACGAAAAAACAATATGG AGATCACATGGACGCCTATGAATGCTACCTCCGCCTTTGGCCCCAACCTGCGCTACATTGTCAAGTGGCG ACGGAGAGAAACCCGAGAGACTTGGAACAATGTCACCGTGTGGGGCTCTCGCTATGTGGTGGGGCAGACC CCTGTCTACGTACCCTATGAGATCCGAGTCCAGGCTGAAAATGACTTTGGGAAAGGCCCGGAGCCTGAAA CCGTCATTGGGTACTCGGGGGAAGATTATCCCAGGGCTGCACCCACTGAAGTTAAAATCCGAGTCCTGAA CAGCACAGCCATCAGCCTTCAGTGGAACCGCGTCTACTCTGACACGGTCCAGGGCCAGCTCAGAGAGTAT CGAGCTTACTACTGGAGGGAAAGCAGTTTGCTGAAGAACCTGTGGGTGTCTCAGAAGAGACAGCAGGCCA GCTTCCCTGGCGACCGCCCCCGGGGCGTGGTGGGTCGCCTGTTCCCCTACAGTAACTACAAGCTGGAGAT GGTTGTGGTCAATGGGAGAGGTGATGGGCCTCGAAGTGAAACCAAGGAATTCACCACCCCGGAAGGAGTA CCCAGTGCCCCCAGGCGTTTCAGAGTCCGACAGCCCAACCTGGAGACCATCAACCTGGAATGGGATCACC CAGAGCACCCCAACGGGATCCTGATTGGATACACGCTCAGATACGTGCCCTTTAATGGAACCAAACTGGG AAAGCAGATGGTGGAAAACTTCTCTCCCAATCAGACCAAGTTCTCGGTGCAGAGAGCAGACCCCGTGTCC CGTTACCGCTTCTCCCTCAGTGCCAGGACGCAGGTGGGCTCTGGAGAAGCAGCCACGGAGGAGTCCCCAA CACCTCCAAATGAAGCTACTCCAACTGCAGCTTACACCAACAACCAGACTGACATCGCCACCCAGGGCTG GTTCATCGGGCTCATGTGTGCCATTGCCCTTCTGGTGCTGATCCTGCTTATCGTCTGCTTCATCAAGAGG AGTCGTGGCGGCAAGTACCCAGTGCGAGAAAAGAAGGATGTCCCCTTGGGCCCTGAAGACCCCAAAGAAG AAGATGGTTCATTTGACTACAGTGACGAGGACAACAAGCCCCTGCAGGGCAGCCAGACATCTCTGGACGG CACCATCAAGCAGCAGGAGAGTGACGACAGCCTAGTGGACTACGGCGAGGGTGGCGAGGGCCAGTTCAAC GAAGATGGCTCCTTTATTGGCCAGTACACTGTCAGAAAGGACAAGGAGGAGACCGAGGGCAATGAGAGCT CAGAGGCCACATCTCCAGTCAATGCCATCTATTCCCTGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217514 representing NM_001160315 Red=Cloning site Green=Tags(s) MARQQAPPWVHVALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWT RNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKEN LDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNAR FHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGD LPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRL VCRANGNPKPTVQWLVNGDPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVL DVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQ GIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYI GNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPG DDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYR TSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQT PVYVPYEIRVQAENDFGKGPEPETVIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREY RAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVGRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGV PSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVS RYRFSLSARTQVGSGEAATEESPTPPNEATPTAAYTNNQTDIATQGWFIGLMCAIALLVLILLIVCFIKR SRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFN EDGSFIGQYTVRKDKEETEGNESSEATSPVNAIYSLA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001160315
ORF Size	3471 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001160315.1, NP_001153787.1
RefSeq Size	9785 bp
RefSeq ORF	3474 bp
Locus ID	116690
MW	130.3 kDa
Gene Summary	This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined.[provided by RefSeq, May 2009]

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