Ddb1 (NM_171995) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217500 representing NM_171995 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGTACAACTACGTCGTAACGGCGCAGAAGCCTACCGCAGTGAACGGCTGTGTGACCGGACACTTTA CTTCAGCGGAAGACTTGAACCTGTTGATTGCTAAAAACACAAGATTAGAGATCTATGTGGTCACCGCAGA AGGCCTACGGCCAGTCAAAGAGGTGGGGATGTATGGGAAGATCGCAGTTATGGAGCTCTTCAGACCCAAG GGGGAGAGTAAAGACCTGCTGTTCATCTTGACTGCTAAGTACAATGCCTGCATCCTGGAGTATAAGCAGA GTGGTGAAAGCATTGATATCATTACAAGGGCCCATGGCAATGTCCAGGACCGTATTGGTCGCCCCTCAGA GACTGGCATCATTGGCATCATTGACCCTGAGTGCCGTATGATTGGCCTCCGACTCTACGATGGCCTTTTC AAGGTCATTCCACTAGACCGAGACAATAAAGAGCTCAAGGCCTTTAACATCCGCCTAGAAGAATTGCATG TCATCGATGTCAAGTTCCTGTATGGTTGTCAAGCGCCTACCATTTGCTTTGTCTACCAGGATCCTCAGGG GCGGCACGTGAAAACCTATGAGGTGTCTCTTCGGGAGAAGGAGTTCAACAAGGGCCCTTGGAAGCAGGAG AATGTAGAAGCCGAAGCTTCAATGGTGATCGCAGTCCCGGAGCCTTTTGGAGGTGCCATCATCATTGGAC AGGAGTCAATTACCTATCACAATGGTGATAAATACCTGGCAATCGCTCCTCCTATCATTAAGCAAAGCAC TATTGTGTGCCATAACCGGGTGGATCCTAATGGCTCACGGTACCTTCTGGGAGATATGGAAGGACGGCTT TTCATGCTGCTCTTAGAGAAGGAGGAGCAGATGGATGGCACCGTCACCCTCAAGGACCTTCGAGTGGAAC TCCTTGGAGAGACCTCCATTGCAGAGTGCCTAACATATCTTGATAACGGTGTTGTGTTTGTCGGGTCTCG CCTGGGTGATTCCCAGCTCGTAAAGCTCAATGTCGACAGCAATGAACAAGGCTCCTATGTAGTGGCTATG GAAACCTTTACCAACTTAGGACCCATTGTGGATATGTGTGTGGTGGACCTGGAGAGGCAGGGACAGGGTC AGCTGGTCACTTGCTCTGGAGCTTTCAAGGAAGGTTCCTTGCGGATCATCCGGAACGGAATTGGAATCCA TGAGCATGCCAGCATTGACTTACCAGGCATCAAAGGTTTATGGCCACTGCGGTCTGACCCAAGTCGGGAG ACTGATGACACTTTGGTACTCTCTTTTGTGGGCCAGACAAGAGTTCTCATGCTAAATGGAGAGGAAGTGG AAGAAACAGAATTGATGGGCTTTGTGGACGATCAGCAGACTTTCTTTTGCGGCAATGTGGCTCATCAGCA GCTTATCCAGATCACCTCAGCGTCTGTAAGGTTGGTCTCTCAAGAGCCCAAAGCTTTGGTCAGCGAGTGG AAGGAGCCCCAGGGCAAGAACATCAGTGTGGCCTCCTGTAACAGCAGCCAGGTGGTAGTTGCTGTGGGAA GGGCACTATACTATCTTCAGATCCATCCTCAGGAGCTCCGGCAAATCAGCCACACAGAGATGGAACATGA AGTGGCTTGCTTGGATATCACCCCACTAGGAGAGAGCAATGGCCTGTCCCCACTTTGTGCCATTGGTCTC TGGACAGATATTTCAGCTCGTATCTTGAAGCTCCCATCCTTTGAACTACTGCACAAGGAGATGCTGGGTG GAGAGATCATCCCTCGATCTATCCTGATGACCACTTTTGAGAGCAGCCACTACCTCCTTTGTGCCTTGGG AGATGGGGCTCTCTTCTACTTTGGGCTCAACATAGAGACAGGCTTACTGAGTGACCGTAAAAAGGTGACT TTGGGTACCCAGCCCACGGTGTTGAGGACATTCCGTTCTCTTTCTACCACCAACGTCTTTGCTTGCTCAG ACCGGCCCACTGTCATCTATAGCAGCAATCATAAGTTGGTCTTCTCCAATGTTAACCTCAAGGAGGTGAA CTATATGTGTCCTCTCAACTCAGATGGCTATCCTGACAGTCTGGCATTGGCCAATAACAGCACTCTCACC ATTGGCACCATCGATGAGATCCAGAAACTCCATATTCGCACGGTTCCTCTCTATGAATCTCCCAGGAAGA TCTGTTATCAGGAAGTGTCTCAGTGCTTTGGGGTCCTTTCCAGCCGCATTGAAGTTCAAGATACCAGTGG AGGCACTACTGCTTTGAGGCCCAGTGCTAGCACCCAGGCCCTGTCCAGCAGCGTCAGCTCCAGCAAGCTC TTCTCTAGTAGTACGGCTCCTCACGAGACCTCCTTTGGAGAAGAGGTGGAAGTGCACAACCTCCTCATCA TTGACCAGCATACTTTTGAAGTGCTTCATGCCCATCAGTTTCTACAAAATGAGTATGCCCTCAGCCTGGT CTCCTGCAAATTGGGTAAAGACCCCAACACATACTTCATTGTGGGCACAGCCATGGTATATCCTGAAGAG GCGGAGCCCAAACAGGGTCGTATTGTGGTCTTCCAGTATTCGGATGGAAAACTACAGACTGTGGCCGAAA AGGAAGTAAAGGGGGCTGTGTACTCTATGGTGGAGTTTAATGGGAAGCTGCTTGCTAGCATCAACAGCAC GGTGCGGCTCTATGAATGGACCACAGAAAAGGAACTTCGCACTGAGTGCAATCACTACAACAACATCATG GCCCTGTACCTGAAGACCAAGGGCGACTTCATCCTGGTGGGCGACCTCATGCGATCAGTGCTACTGCTTG CCTACAAGCCTATGGAAGGAAACTTTGAAGAGATTGCCCGAGACTTTAATCCCAACTGGATGAGTGCAGT GGAGATCTTGGATGATGACAATTTTCTGGGAGCTGAGAATGCCTTTAACTTGTTTGTGTGTCAGAAGGAC AGTGCTGCCACCACTGATGAGGAGCGGCAGCACCTACAGGAGGTTGGTCTCTTCCACCTGGGCGAGTTTG TCAATGTGTTCTGCCATGGCTCCCTAGTTATGCAGAATCTGGGCGAGACTTCTACCCCTACCCAGGGCTC GGTGCTCTTTGGCACAGTCAACGGCATGATAGGGCTGGTGACCTCACTGTCGGAGAGCTGGTACAACCTC CTGTTGGACATGCAGAACCGACTCAATAAGGTCATCAAAAGTGTGGGCAAGATTGAGCACTCCTTCTGGA GATCCTTTCACACTGAGCGAAAGACAGAACCAGCCACAGGCTTCATCGATGGTGACCTGATTGAAAGTTT CCTAGATATCAGCCGCCCCAAGATGCAGGAGGTTGTGGCAAACTTGCAGTATGATGATGGCAGTGGTATG AAGCGGGAGGCAACTGCAGATGACCTCATCAAAGTCGTGGAGGAGCTAACTCGGATCCAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217500 representing NM_171995 Red=Cloning site Green=Tags(s) MSYNYVVTAQKPTAVNGCVTGHFTSAEDLNLLIAKNTRLEIYVVTAEGLRPVKEVGMYGKIAVMELFRPK GESKDLLFILTAKYNACILEYKQSGESIDIITRAHGNVQDRIGRPSETGIIGIIDPECRMIGLRLYDGLF KVIPLDRDNKELKAFNIRLEELHVIDVKFLYGCQAPTICFVYQDPQGRHVKTYEVSLREKEFNKGPWKQE NVEAEASMVIAVPEPFGGAIIIGQESITYHNGDKYLAIAPPIIKQSTIVCHNRVDPNGSRYLLGDMEGRL FMLLLEKEEQMDGTVTLKDLRVELLGETSIAECLTYLDNGVVFVGSRLGDSQLVKLNVDSNEQGSYVVAM ETFTNLGPIVDMCVVDLERQGQGQLVTCSGAFKEGSLRIIRNGIGIHEHASIDLPGIKGLWPLRSDPSRE TDDTLVLSFVGQTRVLMLNGEEVEETELMGFVDDQQTFFCGNVAHQQLIQITSASVRLVSQEPKALVSEW KEPQGKNISVASCNSSQVVVAVGRALYYLQIHPQELRQISHTEMEHEVACLDITPLGESNGLSPLCAIGL WTDISARILKLPSFELLHKEMLGGEIIPRSILMTTFESSHYLLCALGDGALFYFGLNIETGLLSDRKKVT LGTQPTVLRTFRSLSTTNVFACSDRPTVIYSSNHKLVFSNVNLKEVNYMCPLNSDGYPDSLALANNSTLT IGTIDEIQKLHIRTVPLYESPRKICYQEVSQCFGVLSSRIEVQDTSGGTTALRPSASTQALSSSVSSSKL FSSSTAPHETSFGEEVEVHNLLIIDQHTFEVLHAHQFLQNEYALSLVSCKLGKDPNTYFIVGTAMVYPEE AEPKQGRIVVFQYSDGKLQTVAEKEVKGAVYSMVEFNGKLLASINSTVRLYEWTTEKELRTECNHYNNIM ALYLKTKGDFILVGDLMRSVLLLAYKPMEGNFEEIARDFNPNWMSAVEILDDDNFLGAENAFNLFVCQKD SAATTDEERQHLQEVGLFHLGEFVNVFCHGSLVMQNLGETSTPTQGSVLFGTVNGMIGLVTSLSESWYNL LLDMQNRLNKVIKSVGKIEHSFWRSFHTERKTEPATGFIDGDLIESFLDISRPKMQEVVANLQYDDGSGM KREATADDLIKVVEELTRIH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_171995
ORF Size	3420 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_171995.2, NP_741992.2
RefSeq Size	4260 bp
RefSeq ORF	3423 bp
Locus ID	64470
MW	126.9 kDa
Gene Summary	may be a DNA damage repair protein [RGD, Feb 2006]

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