Ccdc150 (NM_001191806) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1304775
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217476 representing NM_001191806 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAACCACAGTGTGCAGACCAGTCCTGTCTCCAACCCACATCAATGCGACAGCTTCAGAAACGTTCA CAGTCCTTCAGCAGAAGATGAGAATAGTTGAGGAACAAACCAGCTCTCTGAGAGATGACCTAATAATGCT AGGATATGGTGACAGACGATGTCAATTACAAACTTCAGACTATTTAGAAGACCCTGTCAGCCAGAAAGTC ACTAGTCCTATTCCAAATGAAGTAGTTTGTCCAGAAAGCAGAGGTATTTTGTGGAAGAACTATGAATTCC TGGCAAATCGAATGTGCTATTTGGAGAACCTCATCCAGCCCTTGAAGATGAACATCTTTCACCTACAAAC TGAAAAGGACCTGAATCCACAGAAAGCAGCGTTTCTGAAGGATCAGCTGAACACAATACAGGGGGAGCAC TTCAAGGACCTGAAGCTGTTGCAACTTGAAGTGATGAATTTGCGCCAGCAACTGAAAGTTGTTAAGGAGG AAGAAGACAGGGCACAAGATGAAGTGCAGAGGTTGACTGCTACTCTGGAAATTGCCACAGAGACAAAGAA GAACGCGGCTGTCATTGAGGAGGAGCTAAAGACCACAAAGCGTAAAATGAACCTTAAAATTCAAGAGCTC AGGCGACAACTGGCTCAGGAGGAGCATGTCAGGGCGTCTCTTGAGAAGTCTGCATCATCCATGCTCCTCA AAGTACATGAGATGGAGTCAGCGGTGGAGGCCGAACGGCAGCAGGTGCACACTTTGCAGCAGAAGTGCAT CGATCTACACAGCTCTATGCAAACAACCCAAGAACTGCTGGCCCAGGAGCAGCAGAAGAGTAACGGCTTG GAGATGACGGCCTCGCAGCTCAAGTCTGATCTAATTTCCAGAGATAACCTGATTTACAAGTTGGTTGAAG AAAATAAGAGTACACAAATGTCTCTCAAGAAGGAACATGAAGAAAATACTTATTTGAAATCTGAAGTACT GTCCCTTCAAGATATATCAGGAAAAGCACAGGTTTTGAATGACCAACTTTCCAAAAAGTGTTCAGAGCTT ACCAGCATGCTTAAGGCTGTTAAACTGGAAAATTCCAGAATTATTGCTGAACATCATGCTACTTTGAAGG TAGAGCAGAAAATGATAACAGAGACATTTCAAGAGCAAAATTTACTGTTAGACACAGCCCATGCCAGCAT CACAGGCGAGCTCCAAACTGTGCAGAATGATAAAGCGCAACTCCAGATGCATCTAGACCATTTAATTCTT GAGCACAATCAGTGTCTCCAGAAAGCACAGGAGGCTGAGAAGAGGACAATGGCACAAAAAGAACTTCTAG AATCAACTATTGCAAGACTACGAGGTGAACTGGAAGCATCAATGCAGGAGAAGAAGTCTCTGCTGGAGAA GAATGAATGGTTTCAGAGGGAGGTTAATAAATCAGAAAAGGGAGTAGCCAAAGAAAAATGCAATTTGGAA AAAGAATTAGCTGAAAGCAAGGAAGACACTAATGTGTTGAATCAAAACCTGCAGATTCTAGTTGAAGAAA ATAAGCATCTGACAGATAAGATGGCTTCCCTTGAACATCACAAAGCCACTTCTGATTACCAAGGGTTTGC TCAGCAGAAAGTGGAGAAGGCCTTGGAAAAAATTACTGAGAGTAAAAATAAGCTTGCCTATGAAAAGGGG AAACTCCAGATAAAAGTCAAACAGCTAGAAGAACAGCTGCACACTTTTACTGAAACCAGGCTGCACAACG ACCACCTACAAAGACAGAATAAGGCTTTAGAGGTCAAGCATGCTCAGGCAACCTCGGAGCGGAGTGCCAG CCAGGCATCTCAGCAGCAGACAGAGGCTCACCTGAGAGAGGAGTTAATAAAATCTCTTCTTGGGAAAAAT GAGGAAGAGCTGGCTGAAGCAGTGAAGTGTCGGGATGCTGCCCTGAAAGAAAGCCAGAAGTTGAAAGGGG ACCTGGAGGCATTGGAGGACAGGGAGAACAAGAAGGTGGGTAACTTTCAGAAACAATTAGCAGAGGCTAA AGAAGACAACTGCAAAGTCACAATCATGCTGGAAAATGTGCTGGCTTCCCACAGTAAGATGCAGGGTGCC CTGGAGAAAGTCCAGATAGAGCTCGGGAGGAGAGATTCAGAGATCGCAGGCCTCAAGAAGGAAAGGTCTC TAAATCAGCAGCGAGTACAGAAGCTTGAAGCGGAGGTGGACCAGTGGCAGGCCAGGATGCTGATTGTGGA GGCCCAGCACAGCAATGAGATGGAGCCACTACAGAAGTCTCTAGATGTAACTAGAGAAGATAACAGGAAA CTGGCCATGAGCCTAGAGCAGGCTCTCCAGACAAATGGCCATCTGCAGTCTAAGCTTGATCACCTCCAAG AAAAACTGGAGAGCAAAGAGCGTGAGCGGCAGAATCTGGAAGCCTTCAAAGAGCGGGTTGCTGAGGAATC CAAAGTAGAAGCAGAATTACATGCAGAACGCATTGAAGCTCTGAGGAAGCAGTTTCAAACCGAGAGAGAG ACTGCAAAGAAAGTATCACATAGGGAAGTTTCTGAGCTGAAGAAAGCTCTTGACGAAGCCAACTTCAGAT CTGTAGAAGTGTCCCGGACAAACCGAGAGCTACGACACAAAGCTACAGAGCTAGAAAAAGTGGTGAACAG TAACAAGGAAAAGCTCAAGAACCAACGGGCCCAGATAAGGATGCACTTGTCAGCCAAAGCAAACAGTGCA CAGAACATGGAGAGGATGAAGCAAATAGAAATGGAATTGAGGCAAATGGAGATAATTAAGGATCAATATC AGAAAAAGAACTATGAACAGTCATTGAGTATCCAGAGATTTGTATCTGAAATGAATGCTCTGCAGAAGGA GATGGAGATGTTAGCTAAGAGCCAGTATGAGACCTCAGCGAGAAATAAACAGCAAGAGTTGAGACTAGTG GCTGAGCGAAAAATAAGGCAGGAGCTAGAAAGCCGATGCAAGGACCTGGAAGACACCATCAGACACCTGA GGAAATGTAAAGAAGCAACAGAGAATAAACTGAAAGAAGCCAGTGTGGAATCTGAACAGATAACAGCTAA TCTGGAAGAAGCTCACCGCTGGTTTAAATGCAGGTTCGATGGATTACAACTTGAGCTGACAAAAAACCGG TTGCAGAGGCTTCCCCGGGAAGACAGGTGGCTGGAAGAGAATCAAGACATGAGGCATAATATTGCAACCA GCCAATCTGTTCTACATCGATGGGAAACTAAACAGAACCTTGGGCTTATGCCCAAGAAGTACCACTCTGA CACAGAGAGGAAGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217476 representing NM_001191806 Red=Cloning site Green=Tags(s) METTVCRPVLSPTHINATASETFTVLQQKMRIVEEQTSSLRDDLIMLGYGDRRCQLQTSDYLEDPVSQKV TSPIPNEVVCPESRGILWKNYEFLANRMCYLENLIQPLKMNIFHLQTEKDLNPQKAAFLKDQLNTIQGEH FKDLKLLQLEVMNLRQQLKVVKEEEDRAQDEVQRLTATLEIATETKKNAAVIEEELKTTKRKMNLKIQEL RRQLAQEEHVRASLEKSASSMLLKVHEMESAVEAERQQVHTLQQKCIDLHSSMQTTQELLAQEQQKSNGL EMTASQLKSDLISRDNLIYKLVEENKSTQMSLKKEHEENTYLKSEVLSLQDISGKAQVLNDQLSKKCSEL TSMLKAVKLENSRIIAEHHATLKVEQKMITETFQEQNLLLDTAHASITGELQTVQNDKAQLQMHLDHLIL EHNQCLQKAQEAEKRTMAQKELLESTIARLRGELEASMQEKKSLLEKNEWFQREVNKSEKGVAKEKCNLE KELAESKEDTNVLNQNLQILVEENKHLTDKMASLEHHKATSDYQGFAQQKVEKALEKITESKNKLAYEKG KLQIKVKQLEEQLHTFTETRLHNDHLQRQNKALEVKHAQATSERSASQASQQQTEAHLREELIKSLLGKN EEELAEAVKCRDAALKESQKLKGDLEALEDRENKKVGNFQKQLAEAKEDNCKVTIMLENVLASHSKMQGA LEKVQIELGRRDSEIAGLKKERSLNQQRVQKLEAEVDQWQARMLIVEAQHSNEMEPLQKSLDVTREDNRK LAMSLEQALQTNGHLQSKLDHLQEKLESKERERQNLEAFKERVAEESKVEAELHAERIEALRKQFQTERE TAKKVSHREVSELKKALDEANFRSVEVSRTNRELRHKATELEKVVNSNKEKLKNQRAQIRMHLSAKANSA QNMERMKQIEMELRQMEIIKDQYQKKNYEQSLSIQRFVSEMNALQKEMEMLAKSQYETSARNKQQELRLV AERKIRQELESRCKDLEDTIRHLRKCKEATENKLKEASVESEQITANLEEAHRWFKCRFDGLQLELTKNR LQRLPREDRWLEENQDMRHNIATSQSVLHRWETKQNLGLMPKKYHSDTERKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191806
ORF Size	3306 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191806.1, NP_001178735.1
RefSeq Size	3309 bp
RefSeq ORF	3309 bp
Locus ID	316399
MW	128.3 kDa

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