Adcy7 (NM_053396) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217474 representing NM_053396 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGCCAAGGGGCGCTACTTCCTAAATGAGGGTGACGAAGGCCCCAAGCAGGCAGCGCTCTATGAGA AGTACAGGCTCACCAGCCAGCACGGGCCACTGCTGCTCCTGCTCCTCCTGGTGGCTGTGGCCACCTGCAT CGCCCTCGTCAGCATCGTCTTCAGCCATGAGGATCTCCGCAGACACCAGGCTGTCCTGGGCACTGCGTTC TTCATGCTGACGCTGTTTGTGGCTCTCTATGTGCTGGTGTATGTTGAGTGCCTAGCACAGCGATGGCTGC GCGTCTTGGCTCTACTCACCTGGGCTTGCCTCATGATACTAGGCTCCGTGCTGATGTGGGACTCCTTGGA GAATGAAGCCTATGCGTGGGAGCAGGTGCCTTTCTTCCTGTTCATCGTCTTTGTGGTGTACGTACTGTTG CCTCTCAGCACGAGGGCAGCTATCGCGGTGGGCGTAGCCTCCACCATCTCCCATCTCCTGGTGTTTGGAG CTGTGACAAGAGCCTTCAAGACATCCATGTCCAGCACACAGCTGGGGCTGCAGCTCCTGGCCAATGCCGT TATCCTTCTGGGTGGGAACTTCACCGGTGCCTTCCACAAGCACCAGCTTCAGGACGCTTCGAGGGATCTC TTCATCTACACCGTCAAGTGTATCCAGATCCGCCGGAAGCTTCGCGTGGAGAAGCGCCAGCAGGAGAACC TGCTGCTTTCAGTGCTCCCAGCTCACATCTCCATGGGTATGAAGCTGGCCATCATTGAACGCCTCAAAGA GGGTGGTGACCGCCACTACACGCCTGACAACAACTTTCACAGCCTCTATGTCAAGCGGCACCAGAACGTC AGCATCTTGTATGCAGACATCGTGGGTTTCACGAGGCTGGCCAGCGACTGCTCTCCCAAGGAGCTAGTGG TGGTGCTCAACGAGCTGTTTGGGAAGTTTGACCAGATTGCCAAGGCCAACGAGTGCATGCGGATCAAGAT CCTGGGTGACTGCTACTACTGCGTGTCAGGCCTGCCTGTGTCGCTGCCCACGCACGCCCGCAACTGTGTG AAGATGGGTCTGGACATATGTGAGGCCATTAAGCAGGTGCGTGAGGCCACCGGAGTGGACATCAGCATGC GTGTGGGCATTCACTCTGGGAATGTCCTGTGTGGGGTCATCGGGCTCCGCAAGTGGCAGTATGACGTGTG GTCCCATGACGTGTCCCTGGCCAACAGGATGGAGGCAGCTGGAGTCCCTGGCCGGGTGCACATCACAGAG GCGACACTGAATCACCTGGACAAGGCATACGAGGTGGAGGACGGGCACGGGGAGCAGCGCGACCCCTATC TGAAAGAGATGAACATCCGTACCTACTTGGTGATTGACCCCCGGAGTCAGCAGCCGCCCCTACCCAGCCA CCACCTGTCCAAGCCCAAGGGGGATGCAACTCTGAAGATGCGGGCTTCCGTGCGTGTAACCCGCTATCTG GAGTCCTGGGGGGCAGCGCGGCCCTTTGCACACCTCAACCACCGGGAGAGCGTGAGCAGCAGTGAGATCC CCATCCCCAACGGACGGAGGCAGAAGGCCATTCCTCTGCGTCGACACCGTGCCCCTGATAGGAGTGCATC CCCCAAGGGACGCCTGGAAGATGACTGTGATGACGAGATGCTGTCAGCCATTGAGGGGCTCAGCTCCACC AGGCCCTGCTGCTCCAAGTCTGATGACTTCCACACCTTTGGTCCCATCTTCCTGGAGAAGGGCTTTGAGC GTGAGTACCGCCTGGTGCCCATCCCCCGGGCTCGCTATGACTTCGCCTGTGCCAGCCTTGTCTTCGTCTG CATCCTGCTTGTCCACCTCCTAGTGATGCCCAGGATGGCAACTCTGGGCTTGTCCTTTGGGCTGGTGGCC TGCCTGCTGGGGCTGGTTCTAAGCTTCTGCTTTGCTACTGAGTTCTCGAGGTGCCTTCCAGCCCGAAGTA CACTCCAGGCCATCTCGGAGAGTGTGGAGACACAGCCCCTGCTCAGACTTTCCCTGGTTGTGGTGACTGT TGGCAGCTTACTGACTGTTGCCATCATCAACATGCCACTGACATTTAACCCAGGCCCAGAGCAGCCTGGA GACAATGAGACAAGCCCACTGGCTGCAAGGAACAGAGTTGGGACCCCATGTGAACTCCTCCCGTACTACA CCTGCAGCTGCGTCCTGGGCTTCATTGCATGCTCTGTTTTCCTGCGGATGAGCCTGGAACTGAAGGCCAT GTTGCTGACGGTGGCCTTGGTGGCCTACCTGCTGCTCTTCAACCTCTCCCCATGCTGGCAGGTCTCAGGC AACAGCACAGAGACCAACAGCACACACAGGACACGTCGGCTCCTGTCTGCAGAACAAAGCATGAACAGCC ACACCCTTGCTCTGGGGGCTCAGATGACTGCCCCCTCCCCCAGCAGTTGTTTAGAGAGAGACCTGAAGAC CATGATCAACTTCTACCTGGTCCTGTTCTATGCCACCCTCATCTTGCTGTCTAGACAGATTGACTATTAC TGCCGCTTGGACTGTCTGTGGAAGAAGAAGTTTAAGAAGGAGCATGAGGAGTTTGAAACAATGGAGAATG TGAACCGCCTCCTCCTAGAGAATGTTCTGCCAGCACATGTGGCTGCCCACTTCATCGGTGACAAGGCAGC TGAGGACTGGTACCATCAATCTTATGACTGTGTCTGTGTCATGTTTGCATCCGTTCCGGACTTCAAAGTG TTCTATACTGAGTGTGATGTCAACAAAGAAGGATTGGAGTGCCTTCGCCTGCTGAATGAGATCATTGCTG ATTTTGACGAGCTGCTGCTGAAGCCCAAGTTCAGTGGTGTGGAGAAGATCAAGACCATTGGCAGCACCTA CATGGCTGCTGCAGGGCTCAGTGTCCCCTCAGGACATGAGAACCAGGACCTGGAGCGGAAGCATGTGCAC ATTGGTGTCTTGGTGGAGTTTAGCATGGCCCTGATGAGCAAGCTGGATGGAATCAACAGGCACTCCTTCA ACTCCTTCCGCCTCCGAGTTGGCATAAACCACGGCCCTGTGATTGCTGGAGTGATTGGCGCACGCAAGCC TCAGTATGACATCTGGGGAAACACAGTCAATGTTGCCAGCCGCATGGAGAGCACCGGAGAGCTTGGGAAG ATCCAGGTTACCGAGGAGACGTGCACCATCCTCCAGGGACTGGGATATTCTTGTGAGTGCCGTGGGCTCA TCAACGTCAAAGGCAAAGGGGAGCTGCGGACTTACTTTGTTTGTACAGACACTGCCAAGTTTCAAGGGCT GGGGCTAAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217474 representing NM_053396 Red=Cloning site Green=Tags(s) MPAKGRYFLNEGDEGPKQAALYEKYRLTSQHGPLLLLLLLVAVATCIALVSIVFSHEDLRRHQAVLGTAF FMLTLFVALYVLVYVECLAQRWLRVLALLTWACLMILGSVLMWDSLENEAYAWEQVPFFLFIVFVVYVLL PLSTRAAIAVGVASTISHLLVFGAVTRAFKTSMSSTQLGLQLLANAVILLGGNFTGAFHKHQLQDASRDL FIYTVKCIQIRRKLRVEKRQQENLLLSVLPAHISMGMKLAIIERLKEGGDRHYTPDNNFHSLYVKRHQNV SILYADIVGFTRLASDCSPKELVVVLNELFGKFDQIAKANECMRIKILGDCYYCVSGLPVSLPTHARNCV KMGLDICEAIKQVREATGVDISMRVGIHSGNVLCGVIGLRKWQYDVWSHDVSLANRMEAAGVPGRVHITE ATLNHLDKAYEVEDGHGEQRDPYLKEMNIRTYLVIDPRSQQPPLPSHHLSKPKGDATLKMRASVRVTRYL ESWGAARPFAHLNHRESVSSSEIPIPNGRRQKAIPLRRHRAPDRSASPKGRLEDDCDDEMLSAIEGLSST RPCCSKSDDFHTFGPIFLEKGFEREYRLVPIPRARYDFACASLVFVCILLVHLLVMPRMATLGLSFGLVA CLLGLVLSFCFATEFSRCLPARSTLQAISESVETQPLLRLSLVVVTVGSLLTVAIINMPLTFNPGPEQPG DNETSPLAARNRVGTPCELLPYYTCSCVLGFIACSVFLRMSLELKAMLLTVALVAYLLLFNLSPCWQVSG NSTETNSTHRTRRLLSAEQSMNSHTLALGAQMTAPSPSSCLERDLKTMINFYLVLFYATLILLSRQIDYY CRLDCLWKKKFKKEHEEFETMENVNRLLLENVLPAHVAAHFIGDKAAEDWYHQSYDCVCVMFASVPDFKV FYTECDVNKEGLECLRLLNEIIADFDELLLKPKFSGVEKIKTIGSTYMAAAGLSVPSGHENQDLERKHVH IGVLVEFSMALMSKLDGINRHSFNSFRLRVGINHGPVIAGVIGARKPQYDIWGNTVNVASRMESTGELGK IQVTEETCTILQGLGYSCECRGLINVKGKGELRTYFVCTDTAKFQGLGLN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_053396
ORF Size	3300 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053396.1, NP_445848.1
RefSeq Size	4164 bp
RefSeq ORF	3303 bp
Locus ID	84420
MW	122.9 kDa

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