Secisbp2l (NM_001168527) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1559930
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217468 representing NM_001168527 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCGAGCGCCGGCCGAGCAGAATGTGAAGCTTTCTGCTGAAGTAGAGCCCTTTATTCCCCAGAAGA AGAATCTGGATGCGTTTGTGCTCCCCATGGCTCTCCCAACTGACAATGGAAGTGTTTCTGGGGTCGAACC AACTCCAATCCCCAGTTATCTGATTACTTGTTACCCATTTGTTCAGGAAAACCAGTCCAATAGACAATTT CCTTTATATAATAATGATATACGATGGCAGCAGCCCAGCCCAAGCCCTACTGGACCCTACCTGGCCTACC CCATTCTATCTGCTCAGCCACCTGTTTCCACAGAGTACACCTATTACCAGCTGATGCCAGCACCCTGTGC CCAAGTTATGGGCTTCTATCATCCTTTCCCTACACCTTACTCCAGCACCTTCCAGGCTGCAAACACTGTC AATGCCATAACCACAGAATGCACCGAGCGTCCAAATCAGCTGGGGCAGCCTTTCCCGTTGTCCAGTCATC GGAGCAGGAATGGTAATAGAGGGCCAGTTGTGCCAAAACCACAGCTTTTACAACAACATATAAAAAACAA AAGACCACAGGTGAAGAACGTGGCTACTCAGAAAGAAACAAGTGCAGCAGGTCCTGATAGCCGATCAAAG ATTGTGCTTCTGGTTGATGCCTCACAGCAAACTGATTTCCCATCAGACATTGCTAATAAGTCTCTCTCGG AGAGCACAGCCACAATGCTCTGGAAAGGCAAAGGCAGGAGGAGAAGAGCGTCCCACCCTGCTGCAGAGTC CTCCAGTGAGCAGGGGGCCAGTGAAGCTGACATTGACAGCGACAGCGGTTACTGCAGCCCCAAGCACAAC AACCAGCCTGCACCAGGGACTCTGCGTGACCCTGCCTCTGGGACCATAAGTCATTCGGAGTCCTCGGGCT GCACAGGTGGTGTAAACTGGCCCAAAGTAACTTGCCAGGCAACTCAGAAAAGACCTTGGATGGAAAAAAA CCAGGCATTTTCTAGAGGTGGAAGGCAAACTGAACAAAGAAATAATTCACAGGTTGGATTCAGATGCCGA GGACACAGTACTTCCTCAGAAAGAAGACAGAATTTGCAAAAAAGACAAGACAATAAGCACTTAAACTCTA CTCAGTCTCACAGAAGTGACCCAAATTCTGAGTCTTTATATTTTGAGGATGAAGATGGGTTTCAAGAACT AAGTGAGAGCGGAAATTCTAAAGATGAGACTATTCAACAGAAACTTTCTTCAAAAGTATTGGATGACCTA CCTGAGAACTCACCCATCAATATAGTTCAGACTCCGATCCCCATTACCACCTCAGTTCCTAAACGAGCTA AGAGTCAGAAGAAAAAGGCCTTAGCTGCAGCCCTTGCCACAGCACAGGAATACTCAGAAATAAGTATGGA GCAAAAAAAGTTGCAGGAAGCTCTATCAAAAGCAGCTGGAAAAAAGAATAAAACCCCAGTGCAGCTGGAT TTAGGGGATATGCTGGCTGCTCTGGAGAAGCAACAGCAGGCAATGAAAGCCCGTCAGATCACCAACACCA GACCGCTGGCACATCCAGTTGTCACCACAGGCACTTTTCACACTAAAGACTCTAACAGAAAAACCCTGAC CAGAAGTCAGCCGTGCTTGACATCCTTTAATTCTCTGGACATTACTTCCTCTAAAGCAAAAAAGGGAAAA GAGAAGGAAATTGCAAAGCTGAAACGACCCACAGCGCTGAAAAAGGTTATTTTGAAAGAAAGAGAGGAGA AGAAAGGACGTCTGACGGTGGAACATAGTGTTTTGGGAGCCGAAGAACTGACGGAGACGAATTTGGACCT AGCCAATGACTTACCTCAGGAGACTATCTCCCAGGAAGATACTGGACTGAGCATGCCCAGTGATGCTTCT CTTTCTCCAGCAAGTCAGAACTCTCCATACTGTATGACACCTGTGTCGCAAGGCTCACCTGCTAGTTCTG GCATAGGTAGTCCAATGGCATCGTCAACGATAACCAAAATCCACAGCAAAAGATTTCGAGAATATTGTAA CCAGGTTCTTTCTAAAGAAATTGATGAATGTGTGACCCTCCTTCTGCAAGAACTTGTCAGTTTCCAGGAA CGCATCTACCAAAAGGACCCTGTGAGAGCCAAAGCCAGACGGCGGCTGGTCATGGGGCTTCGGGAAGTCA CCAAGCACATGAAGCTGAACAAGATCAAGTGCGTCATCATCTCCCCAAACTGTGAGAAGATCCAGTCAAA GGGTGGCCTTGATGAGGCCCTCTACAATGTCATAGCCATGGCCCGGGAGCAAGAGATTCCTTTTGTGTTT GCCCTTGGAAGGAAAGCTCTGGGACGCTGCGTGAACAAGCTGGTTCCTGTTAGTGTTGTAGGAATCTTCA ATTATTTTGGTGCTGAGAGCCTGTTTAATAGATTAGTGGAGCTCACGGAGGAAGCCAGGAAGGCATATAA GGACATGGTTGCCGCAACAGAGCAGGAGCAAGCAGAGGAGGCATTGAGGAGTGTGAAGGCCGTGCCTCAC CACATGGGCCACTCGCGCAACCCCTCGGCAGCAAGCGCCATTTCTTTCTGCAGTGTTATTTCTGAACCCA TTTCTGAAGTAAATGAAAAGGAATATGAAACTAATTGGAGAAGCATGGTGGAGACTTCAGACGGCTTGGA AACATCAGAAAGCGAGAAGGCGGTTCCCTGTAAGCACGACCCTGCTGAGAAGCCCAGCAAACTCACCCCT GACACCACCCAGGTGGGTAAGCAGCCACTGCCGCCCACAGGCAGCATCACCTCAGCGCCAAGCCAGGGGA AACCCACGGTTGACAAGGATGAGCTGAAGCCAGACGACCTGGAGTGGGCCTCGCAGCAGAGCACAGAGAC GGGCTCCTTGGATGGCAGCTGCCGGGATCTTTTGAATTCCTCCATCACCAGCACCACCAGCACTCTTGTT CCTGGCATGCTTGAAGAAGAGGATGATGAGGAGGAGGAGGAGGAAGATTATATTCATGAACCCACGTCAG AAGAAGTACAGCTCAATAGTAGGATTGAGTCCTGGGTCTCCGAGACCCAGAGGACTATGGAGACGCTGCA GCTTGGCAAAGCCCTTCCTGGCTCCGAGGAAGACAGTGCAGAGCAAAGTGGAGAGGAAGCAGTGGAAATG GCCGAGGGGCTGGAGTCGGTGGTCGACAGTGAGACCTGGACTCCTGACCAGCAGCCGAAGCCCAGCAGGA ACGTGAGCAAAGAGCACCCTGATTTCAGTTCCCCTCCCCCAAGTACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217468 representing NM_001168527 Red=Cloning site Green=Tags(s) MDRAPAEQNVKLSAEVEPFIPQKKNLDAFVLPMALPTDNGSVSGVEPTPIPSYLITCYPFVQENQSNRQF PLYNNDIRWQQPSPSPTGPYLAYPILSAQPPVSTEYTYYQLMPAPCAQVMGFYHPFPTPYSSTFQAANTV NAITTECTERPNQLGQPFPLSSHRSRNGNRGPVVPKPQLLQQHIKNKRPQVKNVATQKETSAAGPDSRSK IVLLVDASQQTDFPSDIANKSLSESTATMLWKGKGRRRRASHPAAESSSEQGASEADIDSDSGYCSPKHN NQPAPGTLRDPASGTISHSESSGCTGGVNWPKVTCQATQKRPWMEKNQAFSRGGRQTEQRNNSQVGFRCR GHSTSSERRQNLQKRQDNKHLNSTQSHRSDPNSESLYFEDEDGFQELSESGNSKDETIQQKLSSKVLDDL PENSPINIVQTPIPITTSVPKRAKSQKKKALAAALATAQEYSEISMEQKKLQEALSKAAGKKNKTPVQLD LGDMLAALEKQQQAMKARQITNTRPLAHPVVTTGTFHTKDSNRKTLTRSQPCLTSFNSLDITSSKAKKGK EKEIAKLKRPTALKKVILKEREEKKGRLTVEHSVLGAEELTETNLDLANDLPQETISQEDTGLSMPSDAS LSPASQNSPYCMTPVSQGSPASSGIGSPMASSTITKIHSKRFREYCNQVLSKEIDECVTLLLQELVSFQE RIYQKDPVRAKARRRLVMGLREVTKHMKLNKIKCVIISPNCEKIQSKGGLDEALYNVIAMAREQEIPFVF ALGRKALGRCVNKLVPVSVVGIFNYFGAESLFNRLVELTEEARKAYKDMVAATEQEQAEEALRSVKAVPH HMGHSRNPSAASAISFCSVISEPISEVNEKEYETNWRSMVETSDGLETSESEKAVPCKHDPAEKPSKLTP DTTQVGKQPLPPTGSITSAPSQGKPTVDKDELKPDDLEWASQQSTETGSLDGSCRDLLNSSITSTTSTLV PGMLEEEDDEEEEEEDYIHEPTSEEVQLNSRIESWVSETQRTMETLQLGKALPGSEEDSAEQSGEEAVEM AEGLESVVDSETWTPDQQPKPSRNVSKEHPDFSSPPPST TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001168527
ORF Size	3267 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001168527.1, NP_001161999.1
RefSeq Size	4522 bp
RefSeq ORF	3270 bp
Locus ID	296115
MW	120.1 kDa

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