Map3k9 (NM_001100872) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1562149
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217459 representing NM_001100872 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCCTCCAGATCGCTTCTCGGCTGCCTGGCGAGCGCCGCTGCGGCCCCGCCGGGGGAGGATGCCA CCGGTGCTGGGGCCGAAGAGGAGGAAGACGAAGAGGAGGCGGCGGCCGAGCTGGGATCTCATGCCACGCT GCCCTACTGGACGGCTGTGTTCGAGTACGAGGCGGCAGGAGAGGACGAGCTGACCCTGCGGCTGGGCGAT GTGGTAGAGGTGCTGTCCAAGGACTCTCAGGTGTCCGGCGATGAGGGCTGGTGGACCGGACAGCTGAACC AGCGGGTGGGCATCTTCCCCAGCAACTACGTGACCCCGCGCAGCGCCTTCTCCAGTCGCTGCCAGCCGGG CACGGAGGACCCCAGCTGCTACCCGCCCATTCAGCTGTTGGAGATTGATTTTGCGGAGCTAACCCTGGAG GAGATCATCGGCATTGGAGGCTTTGGGAAAGTCTATCGTGCTTTCTGGGTAGGTGACGAGGTGGCCGTGA AAGCAGCTCGCCACGACCCTGATGAGGACATCAGCCAGACCATAGAGAATGTTCGCCAAGAGGCCAAGCT CTTTGCCATGCTGAAACACCCGAACATCATTGCCCTCAGAGGGGTGTGCCTGAAGGAACCCAACCTCTGC TTGGTCATGGAGTTCGCTCGTGGAGGGCCTCTGAACAGAGTGTTGTCTGGGAAGAGGGTTCCCCCGGACA TCCTGGTGAACTGGGCCGTGCAGATCGCCAGAGGGATGAACTACCTACATGATGAGGCGATCGTGCCCGT CATCCACCGAGACCTTAAGTCCAGCAACATACTGATCCTGCAGAAAGTGGAGAATGGAGACCTGAGTAAC AAGACTCTGAAGATCACTGACTTCGGGCTGGCACGGGAATGGCACCGGACCACCAAGATGAGTGCGGCGG GGACATACGCTTGGATGGCGCCTGAAGTCATCCGCGCTTCCATGTTTTCCAAGGGCAGCGATGTGTGGAG CTATGGAGTACTGCTCTGGGAACTGTTGACTGGCGAGGTGCCCTTCCGGGGCATTGATGGCTTAGCAGTG GCTTACGGTGTGGCAATGAACAAGCTCGCCCTTCCTATCCCTTCTACCTGCCCAGAGCCTTTTGCCAAAC TCATGGAAGACTGCTGGAATCCTGACCCCCACTCACGTCCATCTTTCTCTAGTATCCTGGACCAGCTAAC GACTATAGAAGAGTCTGGTTTCTTTGAGATGCCCAAGGACTCCTTCCACTGCCTGCAGGATGACTGGAAA CATGAGATTCAGGAGATGTTTGACCAACTCAGGGCCAAAGAAAAGGAGCTTCGAACCTGGGAAGAAGAGC TGACCCGGGCTGCGCTCCAGCAGAAGAATCAGGAGGAGCTGCTTCGGCGTAGGGAGCAGGAGCTGGCTGA GCGGGAGATCGACATCCTGGAACGAGAGCTCAACATCATCATCCACCAGCTGTGCCAGGAAAAGCCCCGG GTGAAGAAACGCAAGGGCAAGTTCAGGAAGAGCCGACTGAAGCTCAAGGACGGCAACCGCATCAGCCTTC CCTCGGATTTCCAGCACAAGTTCACGGTGCAGGCCTCCCCGACCATGGATAAAAGGAAGAGTTTGATCAG CAACCGATCGAGTCCTCCTGCAAGCCCCACCATCATCCCTCGCCTTCGAGCCATTCAGTTGACACCAGGT GAAAGTAGTAAAACCTGGGGCCGGAGCTCAGTTGTTCCAAAAGAGGAAGGGGAAGAAGAGGAGAAGAGGG CCCCAAAGAAGAAAGGCCGGACGTGGGGACCAGGGACGCTTGGACAGAAGGAGCTCACGTCAGGAGATGA AGGCCTCAAGTCCCTGGTAGACGGATACAAGCAGTGGTCATCCAGTGCCCCCAACCTGGGGAAGGGCCCG AGGAGTAGCCCTGCCCTGCCCGGGTTCACCAGCCTTATGGAGATAGAGGATGAGGATAGTGAAGGCCCAG GAAGCGGAGAGAATCATCTACAGCATTCACCCAACCAGTCCTACCTCTGTATCCCATTTCCTCGTGGGGA GGATGGGGATGGCCCCTCCAGTGATGGAATTCATGAGGAACCCACCCCAGTCAACTCAGCTACCAGTACC CCTCAGCTGACGCCAACTAACAGCCTCAAGCGCAGCGGGACCCACCACCGGCGCTGCGAGGTCGCTCTGC TCGGATGTGGAGCTGTTCTGGCAGCCACAGGCCTGGGGTTTGACTTGCTGGAAGCTGGCAAGTGCCAGTT GCTTCCCCCAGAGGAACCTGAGCCACCAGCCCGGGAGGAAAAGAAGAGGCGAGAGGGTCTTTTCCAAAGG GCCAGTCGCCCTCGTCGGAGTACCAGCCCGCCCTCCCGAAAGCTCTTTAAGAAGGAAGAGCCGATGACGT TGCTAGGAGACCCCTCTGCCTCCTTGACACTGCTGTCTCTCTCCTCCATCTCTGAATGCAACTCTACTCG CTCCCTACTGCGCTCTGACAGTGATGAGATCGTGGTGTATGAAATGCCAGTCAGCCCAGTTGAAGCTCCA CCCCTAACCCAGTGCACCCAGAACCCCCTGGTTAATGTCCGAGTGGAGCGCTTCAAGAGAGACCCCAACC AGTCCCTGACTCCCACCCACGTCACCCTCACAGCCCCCACACAGCCTAGTGGTCACAGGCGGACTCCTTC TGATGGGGCACTTAAACCAGCATCAGTGCCCCCAGCCCTGGGCAGCAGAAGCCCCTCCAGCAATGGAGTG AGCCCTGGTCCTGGAGCAGGCATGTTGAAAACCCCCAGTCCCAGCCGAGACCCAGGCGAATTTCCCCGTC TCCCTGACCCCAACGTGGTCTTTCCCCCAACTCCGAGGCGCTGGAACACACAGCGAGACTCTACCTTAGA GAGACCCAAGACGCTGGAGTTTCTGCCTCGGCCACGTCCTTCTGCCAACCGGCAGCGGCTGGACCCTTGG TGGTTTGTGTCCCCCAGCCATGCCCGCAGCGCCTCCCCAGCTAATAGCTCCAGCACAGAGACACCCAGCA ACCTGGACTCCTGCTTTGCCAGCAGCAGCAGCACTGTGGAAGAGCGGCCTGGACTCCCAGCCCTGCTCCC CTTACAGGCAGGGCCACTGCCCCCTGCTGAGCGGACACTTCTGGACCTGGACGCTGAGGGGCAGAGCCAG GATAGCACTGTGCCCCTGTGCAGAGCTGAACTGAATGCACATGGGCCTTCTCCATATGAGATCCAACAGG AGTTCTGGTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217459 representing NM_001100872 Red=Cloning site Green=Tags(s) MESSRSLLGCLASAAAAPPGEDATGAGAEEEEDEEEAAAELGSHATLPYWTAVFEYEAAGEDELTLRLGD VVEVLSKDSQVSGDEGWWTGQLNQRVGIFPSNYVTPRSAFSSRCQPGTEDPSCYPPIQLLEIDFAELTLE EIIGIGGFGKVYRAFWVGDEVAVKAARHDPDEDISQTIENVRQEAKLFAMLKHPNIIALRGVCLKEPNLC LVMEFARGGPLNRVLSGKRVPPDILVNWAVQIARGMNYLHDEAIVPVIHRDLKSSNILILQKVENGDLSN KTLKITDFGLAREWHRTTKMSAAGTYAWMAPEVIRASMFSKGSDVWSYGVLLWELLTGEVPFRGIDGLAV AYGVAMNKLALPIPSTCPEPFAKLMEDCWNPDPHSRPSFSSILDQLTTIEESGFFEMPKDSFHCLQDDWK HEIQEMFDQLRAKEKELRTWEEELTRAALQQKNQEELLRRREQELAEREIDILERELNIIIHQLCQEKPR VKKRKGKFRKSRLKLKDGNRISLPSDFQHKFTVQASPTMDKRKSLISNRSSPPASPTIIPRLRAIQLTPG ESSKTWGRSSVVPKEEGEEEEKRAPKKKGRTWGPGTLGQKELTSGDEGLKSLVDGYKQWSSSAPNLGKGP RSSPALPGFTSLMEIEDEDSEGPGSGENHLQHSPNQSYLCIPFPRGEDGDGPSSDGIHEEPTPVNSATST PQLTPTNSLKRSGTHHRRCEVALLGCGAVLAATGLGFDLLEAGKCQLLPPEEPEPPAREEKKRREGLFQR ASRPRRSTSPPSRKLFKKEEPMTLLGDPSASLTLLSLSSISECNSTRSLLRSDSDEIVVYEMPVSPVEAP PLTQCTQNPLVNVRVERFKRDPNQSLTPTHVTLTAPTQPSGHRRTPSDGALKPASVPPALGSRSPSSNGV SPGPGAGMLKTPSPSRDPGEFPRLPDPNVVFPPTPRRWNTQRDSTLERPKTLEFLPRPRPSANRQRLDPW WFVSPSHARSASPANSSSTETPSNLDSCFASSSSTVEERPGLPALLPLQAGPLPPAERTLLDLDAEGQSQ DSTVPLCRAELNAHGPSPYEIQQEFWS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001100872
ORF Size	3231 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001100872.1, NP_001094342.1
RefSeq Size	3638 bp
RefSeq ORF	3234 bp
Locus ID	500690
MW	118.7 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.