Kif11 (NM_001169112) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Knsl1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217443 representing NM_001169112 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCCCAGCCGAGTTCTTCTTCGAAGAAGAAAGAGGAAAAGGGCAAGAACATTCAGGTGGTGGTGA GATGCAGACCATTTAATTTGGCAGAGCGGAAAGCTAACGCCCACTCTGTAGTAGAATGTGATCACGCACG GAAGGAAGTCAGTGTACGAACTGCAGGGCTGACTGACAAGACCTCAAGGAAAACATACACGTTTGACATG GTGTTTGGAGCATCTACAAAACAAATTGATGTTTACCGAAGTGTTGTTTGTCCAATTCTAGATGAAGTTA TTATGGGCTATAATTGTACCATCTTCGCATATGGCCAGACTGGCACTGGAAAAACTTTTACAATGGAAGG TGAAAGGTCACCTAATGAAGTATATACCTGGGAGGAGGATCCTCTGGCTGGTATAATTCCACGTACTCTT CATCAGATTTTTGAGAAACTTACTGATAATGGCACTGAGTTTTCAGTGAAAGTATCCCTATTGGAAATCT ATAATGAGGAGCTTTTTGATCTTCTGAGTCCGTCTTCTGATGTTTCTGAAAGGCTGCAGATGTTTGATGA TCCCCGGAACAAGAGAGGAGTGATAATCAAAGGCTTAGAGGAAATCACAGTACACAACAAAGATGAAGTC TACCAAATCTTAGAGAAGGGGGCAGCCAAAAGGACAACTGCAGCAACCTTGATGAATGCTTACTCCAGTC GTTCACACTCAGTTTTTTCTGTCACGATACACATGAAAGAAACGACAATTGATGGAGAAGAGCTTGTTAA AATTGGCAAGTTGAATTTGGTTGATCTTGCAGGAAGTGAAAATATTGGGCGTTCTGGAGCTGTTGACAAG AGGGCCCGGGAAGCTGGAAATATCAACCAGTCCCTGCTGACTCTGGGGAGAGTTATCACTGCTCTTGTGG AAAGAACACCTCATATTCCTTATCGAGAATCTAAACTAACAAGAATCCTGCAAGATTCTCTTGGGGGGCG TACAAGGACATCTATAATTGCAACCATTTCCCCTGCGTCTCTCAATCTTGAGGAAACTCTGAGTACATTG GAATATGCTCACAGAGCAAAGAACATAATGAATAAGCCTGAAGTTAATCAGAAACTCACCAAAAAGGCAC TTATTAAGGAGTATACAGAAGAGATAGAGCGTTTGAAGCGAGATCTTGCAGCAGCTCGTGAGAAAAATGG AGTGTACATCTCTGAAGAAAGTTTCAGAGCCATGAATGGAAAAGTAACTGTTCAGGAGGAACAAATTGCT GAGTTGGCTGAAAAAATTGGTGTCCTTGAGGAGGAGCTCAGTAAGGCTGCAGAGTTATTTACGGATAGTG AAAAGGAACTTAATCAGTGTAAATCTGACCTGCAAACCAAGACACAGGAACTTGAAACCACTCAGAAACA TTTGCAAGAAACAAAATTACAACTTGTTAAAGAGGAGTATGTCTCTTCAGCTTTGGAAAGAACTGAGAAG AAGCTTCATGAAACGGCCAGCAAGTTGCTTAACACAGTTAAAGAAACCACCAGGGACGTATCTGGTCTGC ATTCTAAACTGGACCGGAAGAGAGCAATCGATGCGCACAACGCTGAGGCTCAGGAGAGCTTTGGCAGGAG CCTCAGCAGTCTGTTTAATAATATGGAAGAGTTGATCAGGGACGGCAGTGAGAAACAGAAGGCCATGCTG GACGTTCACAAAGCACTGTTCGGTAACCTGATGTCTTCTAGTGTCTCTGCATTAGACACTATTTCCACGA CAGCACTTGAATCTCTCATGTCTATTCCAGAAAACGTGTTAGCTCGTGTTTCTCAGATTTCTGATATGAT ATTGGAAGAGCAGTCGTTAGCAGCCCAGAGTAAAACTGTGCTGCAAGGATCGATTGATGAACTTGTGACC AACCTTTTCACGTCCCTGAAGACCATCATAGCTCCTGGTGTGGTGTCCATCTTAAACATAAATAGGCAAC TACAGCATATTTTCAGGGCTTCGGTGACAATGGCTGAAAAGGTAGAAGATCAAAAAAGAGAAATAGACAG TTTTCTCAGCATATTGTGTAACAATTTACATGAACTCCGAGAAAACACTGTTTCTTCCTTGGTTGAATCA CAAAAGCTTTGTGGAAACCTAACTGAAGACCTGAAGACAATAAAGGAAACCCATTCACAGGAACTTTGCC AGTTAAGCAGTAGTTGGGCAGAGAGATTCTGTGCTTTGGAGAAGAAGTATGCAAACATCCAGAAACCACT GAACAGTATTCAAGAAAACACAGAGCGGAAGTCTACTGATATAATCAATAAAACAACAGTTCACAGTAAG AAAATTCTTGCAGACTCTGATGGATTATTACAAGAACTCAGACACTTTAACGAAGAAGGTACACAGTTGG TTGAAGACTCTGTAGGACACTGTGGTTCACTCAGCAGCAACCTGGAGACTGTATCCCAAGAGATCACACA GAGGTGTGGGACCCTGAACACAAGCACAGTTCATTTTTCTGATCAGTGGGCATCCTGCTTAAGCAAGAGG AAGGAAGAGCTTCAGAACTTAATGGAGTTTGTAAATGGATGTTGTGAAGCTTCAAGTTCAGAGATCACTG AAAAAATAAGAGGACACAGAGCAGCTATTGAGAGCCAGCACAGCTCCTTTGTTGTTCAGATAACTTCTGA TGAAGAAAAATTTAAAGCTGGAAGCCTAGAGCTTGATAAAACTATAAAAACTGGTTTAACAAAGCTTAAT TGCTTTCTGCAACAGGATCTGAAACTAGATATCCCAACAGGTATGACACCAGAGAGGAAAAATTATTTAT ATCCATCAACACTTGTGAGAACTGAACCACGGGAGCAACTCCTTGATCAGCTGCAAAAGAAACAGCCCAA GCCACTGATGATGCTAAATTGCTCAGAAAGCAGCAAAGAGACCAGTCAGGACATGGAGGAGGACAGGGAG GTTCTGGAGCAGAGTACTGAAGAACTCGTAAGTCAAGAGCCGTGTGTACATACCAGTGCGGATTGCTCTT CCAGTGGAGGTGTTCCATTTTTTCAGCATAAAAAGCCACATGGAAAAGACAAAGAAAACAGAGGCCTTAA CCCCGTGGAGAAGGGTAAAGTGGAGGAGGCCTCGGAGCACTCCATCTCCAAGAGCAGACTGCCGCTGCGC GCCTCCATCAACCTCTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217443 representing NM_001169112 Red=Cloning site Green=Tags(s) MASQPSSSSKKKEEKGKNIQVVVRCRPFNLAERKANAHSVVECDHARKEVSVRTAGLTDKTSRKTYTFDM VFGASTKQIDVYRSVVCPILDEVIMGYNCTIFAYGQTGTGKTFTMEGERSPNEVYTWEEDPLAGIIPRTL HQIFEKLTDNGTEFSVKVSLLEIYNEELFDLLSPSSDVSERLQMFDDPRNKRGVIIKGLEEITVHNKDEV YQILEKGAAKRTTAATLMNAYSSRSHSVFSVTIHMKETTIDGEELVKIGKLNLVDLAGSENIGRSGAVDK RAREAGNINQSLLTLGRVITALVERTPHIPYRESKLTRILQDSLGGRTRTSIIATISPASLNLEETLSTL EYAHRAKNIMNKPEVNQKLTKKALIKEYTEEIERLKRDLAAAREKNGVYISEESFRAMNGKVTVQEEQIA ELAEKIGVLEEELSKAAELFTDSEKELNQCKSDLQTKTQELETTQKHLQETKLQLVKEEYVSSALERTEK KLHETASKLLNTVKETTRDVSGLHSKLDRKRAIDAHNAEAQESFGRSLSSLFNNMEELIRDGSEKQKAML DVHKALFGNLMSSSVSALDTISTTALESLMSIPENVLARVSQISDMILEEQSLAAQSKTVLQGSIDELVT NLFTSLKTIIAPGVVSILNINRQLQHIFRASVTMAEKVEDQKREIDSFLSILCNNLHELRENTVSSLVES QKLCGNLTEDLKTIKETHSQELCQLSSSWAERFCALEKKYANIQKPLNSIQENTERKSTDIINKTTVHSK KILADSDGLLQELRHFNEEGTQLVEDSVGHCGSLSSNLETVSQEITQRCGTLNTSTVHFSDQWASCLSKR KEELQNLMEFVNGCCEASSSEITEKIRGHRAAIESQHSSFVVQITSDEEKFKAGSLELDKTIKTGLTKLN CFLQQDLKLDIPTGMTPERKNYLYPSTLVRTEPREQLLDQLQKKQPKPLMMLNCSESSKETSQDMEEDRE VLEQSTEELVSQEPCVHTSADCSSSGGVPFFQHKKPHGKDKENRGLNPVEKGKVEEASEHSISKSRLPLR ASINLC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001169112
ORF Size	3168 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001169112.1, NP_001162583.1
RefSeq Size	4731 bp
RefSeq ORF	3171 bp
Locus ID	171304
MW	118.3 kDa
Gene Summary	participates in motile activities within the cell [RGD, Feb 2006]

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