Atp1a4 (NM_001271030) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217422 representing NM_001271030 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCTGGAAAAGAGACAGCGGCCACTAGTGAGCAGAAGCCAAGACCCACCCTTAGGGCTTCAAATA CGAATAGACAGCCGAAAGTGAAAAGAAGGAAGAAAGATTTAGAGGAGTTGAAGAAGGAAGTGGTTATGGA CGATCACAAGCTAACGTTGGATGAGCTGAGTGCCAAGTACTCTGTGGACTTGACCAAGGGTCTTAGCGTC ACAGATGCCCAGGAAATTCTGACTCTAAATGGACCGAATGTACTAACCCCTCCTCCAACCACTCCAGAAT GGATCAAATTCTGTAAACAATTATTTGGTGGCTTCTCCCTCCTGCTGTGGACTGGTTCCTTGCTCTGCTT CCTGGCCTATGGCATACATGTATCTTATTACCAAGAAAACGCCAACAAAGATAATCTGTACCTGGGTATT GTACTATCTGCCGTGGTCATCATCACTGGCTGCTTCTCATATTACCAGGAGGCGAAGAGCTCAAAGATCA TGGAGTCTTTTAAGACCATGGTGCCTCAGCAAGCTCTGGTAATTCGAGATGGAGAGAAAATGCAGATCAA TGTAAGAGATGTGGTTTTGGGAGACCTAGTAGAAGTGAAGGGTGGAGACCAAGTCCCTGCTGATATCAGG GTTATCGCTGCGCAAGGATGTAAGGTAGACAATTCCTCCTTGACTGGGGAGTCAGAACCACAGTCTCGTT GCCCTGATTGCACCCATGAGAACCCTCTGGAGACACGGAATATCATCTTCTTCTCCACCAACTGCGTAGA AGGAACAGCCCGGGGTGTTGTGATTGCTACAGGAGACCACACAGTAATGGGCCGGATTGCTTCCCTGACA TCAGGATTGACAATGGGCAAGACCCCGATTGCCACTGAAATCGAACACTTCATCCATATAATCACAGCTG TCGCAGTCTTCCTCGGTGTCACGTTTTTTTTCCTCTCACTTATCTTGGGCTATACCTGGCTGGATGCTGT CATTTTCCTTATTGGCATCATTGTGGCCAATGTGCCAGAAGGGCTGCTGGCCACTGTCACTGTGTGCCTG ACGCTGACAGCCAAGCGCATGGCTCGCAAGAACTGCCTGGTGAAGAACCTGGAGGCGGTGGAGACGCTGG GCTCCACGTCCACCATCTGCTCGGACAAGACTGGCACCCTCACCCAGAACCGCATGACGGTGGCTCATCT GTGGTTTGATAAGACTGTGTATGAGGCTGACACCAGCGAGGAGCAAACCACGGGGAAAACATTTCCCAAG TCCTCTGACACCTGGTTTTACCTGGCTCGAATTGCTGGCCTCTGCAACCGGGCCGACTTTAAGCCTCATC AGGAGTCTCTTCCCATAACTAAGCGGACAACAACAGGGGATGCTTCTGAGTCAGCCCTCCTCAAATTCAT TGAGCAGTCTTACAGCCCTGTGAGTGAAATGAGACAGAAAAACCCCAAGGTGGCAGAGATTCCTTTTAAT TCTACCAACAAGTACCAGATGTCTATTCACCTTCTGGAAGATAACTCAGAAGCCCACGTGCTGCTGATGA AAGGTGCTCCAGAAAGGATCTTGGATTTTTGCTCCAGTTTCCTGCTCAATGGGCAGGAGTACCCTATGGA CGAAGAAATGAAGACAGACTTCCAGAATGCCTACATAGAACTGGGAGGTCTGGGCGAGCGTGTGCTGGGG TTCTGCTTCTTGAATTTGCCTAGCAATTTCTCTAAGGGATTCCAATTTAATACCGAAGAATTAAATTTTC CCATGGAGAATCTGTGTTTCGCCGGCCTCATATCTATGATCGACCCTCCTCGAACGGCTGTGCCTGATGC TGTTAGCAAGTGCCGAAGTGCAGGGATTAAGGTAATCATGGTAACAGGGGATCATCCCATCACAGCCAAA GCTATTGCCAAAAGTGTGGGCATCATTTCAGAAGCTAATGAAACGGCGGAGGACATCGCTGCTCGGCTTA ATATCTCCATCAGTCAAGTCAGTAACAAGTCTATTAAAGCCATTGTAGTGCACGGCTCAGAGCTGAAGGA CATGGACTCAGGTCAGCTGGACAATATCCTCAAGTCCTACAAGGAGATCGTGTTTGCGCGGACCTCTCCT CAGCAGAAGCTCATCATCGTGGAGGGCTGTCAGAGACTGGGAGCCATTGTGGCAGTGACTGGTGACGGGG TGAACGACTCCCCCGCGCTGAAGAAGGCTGACATCGGCATTGCCATGGGCATCACTGGCTCTGATGTCTC TAAGCAGGCGGCTGACATGATCCTTCTCGACGACAACTTTGCCTCCATTGTGACGGGCGTGGAGGAGGGT CGCCTCATCTTTGACAACCTGAAGAAGTCCATCGCCTATACCCTGACCAGCAACATCCCTGAGATCACCC CCTTCCTGCTGTTCATCGTCTTAAGTATACCCTTGCCTCTGGGTACCATTACCATTCTCTGCATTGACCT GGGTACTGACATGGTCCCTGCTATCTCCTTGGCTTATGAGACACCTGAAAGTGACATCATGAAAAGGCTT CCACGGAACCCCAAAACTGACAATCTCGTGAACGACCGTCTTATCGGCATGGCCTATGGGCAGATCGGGA TGATACAGGCGCTGGCTGGATTTTTCACCTATTTCGTGATCCTGGCGGAGAATGGTTTTAAGCCTCTTGA TCTTCTGGGTATCCGTCTATACTGGGATGATACAAACCTAAATGACCTAGAGGACACCTATGGGCAGCAG TGGACCTATGAGCAGCGGAAGGTGGTGGAGTTCACATGCCAAACAGCCTTCTTCATCAGCATCGTGATCG TGCAGTGGGCTGACCTCATCATCTGCAAGACGCGCAGAAATTCACTCTTCAAGCAGGGCATGAAAAACAA AGTCCTAATATTTGGACTCCTTGAGGAGACGATCCTGGCAGCTTGCCTGTCCTACATTCCAGGCATGGAT GTAGCCCTGAGAATGTACCCACTCAAGATAAACTGGTGGTTCTGCGCACTTCCCTACAGTGTTCTCATCT TCATCTATGATGAAGTTCGAAAACTCATCATCCGCAGACGGCCCGGTGGATGGCTGGAGAAGGAGACGTA CTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217422 representing NM_001271030 Red=Cloning site Green=Tags(s) MEPGKETAATSEQKPRPTLRASNTNRQPKVKRRKKDLEELKKEVVMDDHKLTLDELSAKYSVDLTKGLSV TDAQEILTLNGPNVLTPPPTTPEWIKFCKQLFGGFSLLLWTGSLLCFLAYGIHVSYYQENANKDNLYLGI VLSAVVIITGCFSYYQEAKSSKIMESFKTMVPQQALVIRDGEKMQINVRDVVLGDLVEVKGGDQVPADIR VIAAQGCKVDNSSLTGESEPQSRCPDCTHENPLETRNIIFFSTNCVEGTARGVVIATGDHTVMGRIASLT SGLTMGKTPIATEIEHFIHIITAVAVFLGVTFFFLSLILGYTWLDAVIFLIGIIVANVPEGLLATVTVCL TLTAKRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHLWFDKTVYEADTSEEQTTGKTFPK SSDTWFYLARIAGLCNRADFKPHQESLPITKRTTTGDASESALLKFIEQSYSPVSEMRQKNPKVAEIPFN STNKYQMSIHLLEDNSEAHVLLMKGAPERILDFCSSFLLNGQEYPMDEEMKTDFQNAYIELGGLGERVLG FCFLNLPSNFSKGFQFNTEELNFPMENLCFAGLISMIDPPRTAVPDAVSKCRSAGIKVIMVTGDHPITAK AIAKSVGIISEANETAEDIAARLNISISQVSNKSIKAIVVHGSELKDMDSGQLDNILKSYKEIVFARTSP QQKLIIVEGCQRLGAIVAVTGDGVNDSPALKKADIGIAMGITGSDVSKQAADMILLDDNFASIVTGVEEG RLIFDNLKKSIAYTLTSNIPEITPFLLFIVLSIPLPLGTITILCIDLGTDMVPAISLAYETPESDIMKRL PRNPKTDNLVNDRLIGMAYGQIGMIQALAGFFTYFVILAENGFKPLDLLGIRLYWDDTNLNDLEDTYGQQ WTYEQRKVVEFTCQTAFFISIVIVQWADLIICKTRRNSLFKQGMKNKVLIFGLLEETILAACLSYIPGMD VALRMYPLKINWWFCALPYSVLIFIYDEVRKLIIRRRPGGWLEKETYY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271030
ORF Size	3084 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271030.1, NP_001257959.1
RefSeq Size	3491 bp
RefSeq ORF	3087 bp
Locus ID	29132
UniProt ID	Q64541
MW	114 kDa
Gene Summary	alpha subunit isoform of Na+, K(+)-ATPase, which has sodium/potassium ion exchange activity; may be involved in regulation of intracellular hydrogen ion homeostasis and sperm motility [RGD, Feb 2006]

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